This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.65 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 196,090 (6.9%) Reads that were too short: 119,460 (4.2%) Reads written (passing filters): 76,630 (2.7%) Total basepairs processed: 858,556,748 bp Total written (filtered): 17,493,206 bp (2.0%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 196090 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1075 696.4 0 1075 7 754 696.4 0 754 8 20465 696.4 0 20465 9 18252 696.4 0 18252 10 146 696.4 0 146 11 118 696.4 0 118 12 48 696.4 0 48 13 73 696.4 0 73 14 67 696.4 0 67 15 44 696.4 0 44 16 47 696.4 0 47 17 81 696.4 0 81 18 56 696.4 0 56 19 41 696.4 0 41 20 52 696.4 0 52 21 73 696.4 0 73 22 27 696.4 0 27 23 30 696.4 0 30 24 46 696.4 0 46 25 29 696.4 0 29 26 61 696.4 0 61 27 44 696.4 0 44 28 42 696.4 0 42 29 38 696.4 0 38 30 54 696.4 0 54 31 54 696.4 0 54 32 43 696.4 0 43 33 40 696.4 0 40 34 44 696.4 0 44 35 36 696.4 0 36 36 27 696.4 0 27 37 47 696.4 0 47 38 87 696.4 0 87 39 28 696.4 0 28 40 40 696.4 0 40 41 48 696.4 0 48 42 43 696.4 0 43 43 59 696.4 0 59 44 66 696.4 0 66 45 56 696.4 0 56 46 52 696.4 0 52 47 149 696.4 0 149 48 103 696.4 0 103 49 52 696.4 0 52 50 52 696.4 0 52 51 42 696.4 0 42 52 54 696.4 0 54 53 66 696.4 0 66 54 227 696.4 0 227 55 96 696.4 0 96 56 32 696.4 0 32 57 73 696.4 0 73 58 90 696.4 0 90 59 36 696.4 0 36 60 51 696.4 0 51 61 50 696.4 0 50 62 64 696.4 0 64 63 32 696.4 0 32 64 36 696.4 0 36 65 44 696.4 0 44 66 39 696.4 0 39 67 62 696.4 0 62 68 108 696.4 0 108 69 64 696.4 0 64 70 83 696.4 0 83 71 38 696.4 0 38 72 57 696.4 0 57 73 80 696.4 0 80 74 60 696.4 0 60 75 125 696.4 0 125 76 62 696.4 0 62 77 64 696.4 0 64 78 100 696.4 0 100 79 56 696.4 0 56 80 42 696.4 0 42 81 77 696.4 0 77 82 78 696.4 0 78 83 40 696.4 0 40 84 54 696.4 0 54 85 55 696.4 0 55 86 51 696.4 0 51 87 55 696.4 0 55 88 66 696.4 0 66 89 48 696.4 0 48 90 59 696.4 0 59 91 143 696.4 0 143 92 83 696.4 0 83 93 75 696.4 0 75 94 76 696.4 0 76 95 80 696.4 0 80 96 81 696.4 0 81 97 236 696.4 0 236 98 138 696.4 0 138 99 146 696.4 0 146 100 47 696.4 0 47 101 55 696.4 0 55 102 31 696.4 0 31 103 51 696.4 0 51 104 39 696.4 0 39 105 55 696.4 0 55 106 76 696.4 0 76 107 95 696.4 0 95 108 63 696.4 0 63 109 64 696.4 0 64 110 54 696.4 0 54 111 54 696.4 0 54 112 78 696.4 0 78 113 86 696.4 0 86 114 80 696.4 0 80 115 107 696.4 0 107 116 113 696.4 0 113 117 125 696.4 0 125 118 120 696.4 0 120 119 174 696.4 0 174 120 191 696.4 0 191 121 125 696.4 0 125 122 76 696.4 0 76 123 99 696.4 0 99 124 140 696.4 0 140 125 1157 696.4 0 1157 126 809 696.4 0 809 127 878 696.4 0 878 128 118 696.4 0 118 129 108 696.4 0 108 130 115 696.4 0 115 131 85 696.4 0 85 132 84 696.4 0 84 133 115 696.4 0 115 134 166 696.4 0 166 135 283 696.4 0 283 136 237 696.4 0 237 137 118 696.4 0 118 138 121 696.4 0 121 139 125 696.4 0 125 140 95 696.4 0 95 141 72 696.4 0 72 142 117 696.4 0 117 143 139 696.4 0 139 144 138 696.4 0 138 145 880 696.4 0 880 146 644 696.4 0 644 147 845 696.4 0 845 148 96 696.4 0 96 149 111 696.4 0 111 150 109 696.4 0 109 151 128 696.4 0 128 152 124 696.4 0 124 153 115 696.4 0 115 154 177 696.4 0 177 155 188 696.4 0 188 156 178 696.4 0 178 157 202 696.4 0 202 158 210 696.4 0 210 159 163 696.4 0 163 160 115 696.4 0 115 161 155 696.4 0 155 162 144 696.4 0 144 163 139 696.4 0 139 164 177 696.4 0 177 165 323 696.4 0 323 166 312 696.4 0 312 167 252 696.4 0 252 168 139 696.4 0 139 169 126 696.4 0 126 170 124 696.4 0 124 171 206 696.4 0 206 172 272 696.4 0 272 173 1184 696.4 0 1184 174 3218 696.4 0 3218 175 1772 696.4 0 1772 176 946 696.4 0 946 177 117 696.4 0 117 178 144 696.4 0 144 179 179 696.4 0 179 180 202 696.4 0 202 181 577 696.4 0 577 182 611 696.4 0 611 183 540 696.4 0 540 184 213 696.4 0 213 185 320 696.4 0 320 186 1352 696.4 0 1352 187 1010 696.4 0 1010 188 1350 696.4 0 1350 189 393 696.4 0 393 190 186 696.4 0 186 191 172 696.4 0 172 192 197 696.4 0 197 193 218 696.4 0 218 194 191 696.4 0 191 195 187 696.4 0 187 196 133 696.4 0 133 197 88 696.4 0 88 198 101 696.4 0 101 199 174 696.4 0 174 200 163 696.4 0 163 201 197 696.4 0 197 202 196 696.4 0 196 203 201 696.4 0 201 204 192 696.4 0 192 205 156 696.4 0 156 206 598 696.4 0 598 207 657 696.4 0 657 208 858 696.4 0 858 209 375 696.4 0 375 210 164 696.4 0 164 211 176 696.4 0 176 212 253 696.4 0 253 213 297 696.4 0 297 214 367 696.4 0 367 215 332 696.4 0 332 216 198 696.4 0 198 217 208 696.4 0 208 218 247 696.4 0 247 219 337 696.4 0 337 220 420 696.4 0 420 221 363 696.4 0 363 222 290 696.4 0 290 223 231 696.4 0 231 224 408 696.4 0 408 225 4007 696.4 0 4007 226 3194 696.4 0 3194 227 4250 696.4 0 4250 228 310 696.4 0 310 229 539 696.4 0 539 230 3440 696.4 0 3440 231 3299 696.4 0 3299 232 4132 696.4 0 4132 233 1517 696.4 0 1517 234 450 696.4 0 450 235 315 696.4 0 315 236 178 696.4 0 178 237 181 696.4 0 181 238 172 696.4 0 172 239 172 696.4 0 172 240 218 696.4 0 218 241 238 696.4 0 238 242 196 696.4 0 196 243 217 696.4 0 217 244 172 696.4 0 172 245 282 696.4 0 282 246 1003 696.4 0 1003 247 514 696.4 0 514 248 701 696.4 0 701 249 289 696.4 0 289 250 789 696.4 0 789 251 607 696.4 0 607 252 1488 696.4 0 1488 253 2330 696.4 0 2330 254 1650 696.4 0 1650 255 756 696.4 0 756 256 146 696.4 0 146 257 131 696.4 0 131 258 118 696.4 0 118 259 158 696.4 0 158 260 178 696.4 0 178 261 211 696.4 0 211 262 197 696.4 0 197 263 228 696.4 0 228 264 260 696.4 0 260 265 378 696.4 0 378 266 238 696.4 0 238 267 259 696.4 0 259 268 293 696.4 0 293 269 337 696.4 0 337 270 255 696.4 0 255 271 573 696.4 0 573 272 317 696.4 0 317 273 556 696.4 0 556 274 270 696.4 0 270 275 578 696.4 0 578 276 498 696.4 0 498 277 420 696.4 0 420 278 652 696.4 0 652 279 1963 696.4 0 1963 280 6074 696.4 0 6074 281 6629 696.4 0 6629 282 4055 696.4 0 4055 283 1207 696.4 0 1207 284 433 696.4 0 433 285 697 696.4 0 697 286 415 696.4 0 415 287 394 696.4 0 394 288 361 696.4 0 361 289 416 696.4 0 416 290 460 696.4 0 460 291 417 696.4 0 417 292 323 696.4 0 323 293 292 696.4 0 292 294 438 696.4 0 438 295 736 696.4 0 736 296 1006 696.4 0 1006 297 1374 696.4 0 1374 298 1773 696.4 0 1773 299 2744 696.4 0 2744 300 5778 696.4 0 5778 301 29094 696.4 0 29094 Finished in 3.68 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 76,630 Reads with adapters: 19,526 (25.5%) Reads that were too short: 50 (0.1%) Reads written (passing filters): 19,476 (25.4%) Total basepairs processed: 17,493,206 bp Total written (filtered): 5,232,582 bp (29.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 19526 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 15 0.0 3 0 0 9 5 1 19 215 0.0 3 0 186 15 10 4 20 18423 0.0 4 17204 961 191 51 16 21 62 0.0 4 5 49 6 2 22 10 0.0 4 3 3 3 0 1 23 128 0.0 4 0 0 1 93 34 25 1 0.0 4 1 35 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 40 2 0.0 4 2 41 2 0.0 4 1 0 0 0 1 42 1 0.0 4 1 45 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 121 2 0.0 4 0 0 0 0 2 123 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 8 0.0 4 0 0 0 0 8 127 591 0.0 4 0 0 0 0 591 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 130 4 0.0 4 0 0 0 0 4 132 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 4 0.0 4 0 0 0 0 4 167 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 203 2 0.0 4 0 0 0 1 1 204 1 0.0 4 0 0 0 0 1 206 2 0.0 4 0 0 0 0 2 225 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 2 0.0 4 0 0 0 0 2 230 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 0 2 240 2 0.0 4 0 0 0 0 2 241 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 287 1 0.0 4 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.72 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 19,476 Reads with adapters: 4,964 (25.5%) Reads written (passing filters): 19,476 (100.0%) Total basepairs processed: 5,232,582 bp Total written (filtered): 5,182,112 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4964 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.4% C: 8.6% G: 86.9% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 15 304.3 0 15 4 8 76.1 0 8 5 1 19.0 0 1 6 25 4.8 0 25 8 2 0.3 0 2 9 2012 0.1 0 256 1756 10 2460 0.0 1 240 2220 11 396 0.0 1 8 388 12 15 0.0 1 0 15 13 15 0.0 1 0 15 79 1 0.0 1 1 89 1 0.0 1 1 150 1 0.0 1 0 1 151 1 0.0 1 0 1 189 1 0.0 1 0 1 208 7 0.0 1 4 3 223 3 0.0 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.