This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.38 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 192,096 (6.7%) Reads that were too short: 121,282 (4.3%) Reads written (passing filters): 70,814 (2.5%) Total basepairs processed: 858,556,748 bp Total written (filtered): 17,120,865 bp (2.0%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 192096 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 845 696.4 0 845 7 687 696.4 0 687 8 18734 696.4 0 18734 9 21145 696.4 0 21145 10 109 696.4 0 109 11 108 696.4 0 108 12 48 696.4 0 48 13 84 696.4 0 84 14 85 696.4 0 85 15 53 696.4 0 53 16 47 696.4 0 47 17 72 696.4 0 72 18 722 696.4 0 722 19 48 696.4 0 48 20 69 696.4 0 69 21 103 696.4 0 103 22 32 696.4 0 32 23 29 696.4 0 29 24 64 696.4 0 64 25 39 696.4 0 39 26 49 696.4 0 49 27 48 696.4 0 48 28 57 696.4 0 57 29 29 696.4 0 29 30 72 696.4 0 72 31 74 696.4 0 74 32 30 696.4 0 30 33 46 696.4 0 46 34 39 696.4 0 39 35 47 696.4 0 47 36 41 696.4 0 41 37 49 696.4 0 49 38 84 696.4 0 84 39 40 696.4 0 40 40 51 696.4 0 51 41 38 696.4 0 38 42 41 696.4 0 41 43 63 696.4 0 63 44 51 696.4 0 51 45 69 696.4 0 69 46 1424 696.4 0 1424 47 190 696.4 0 190 48 119 696.4 0 119 49 47 696.4 0 47 50 51 696.4 0 51 51 42 696.4 0 42 52 68 696.4 0 68 53 36 696.4 0 36 54 214 696.4 0 214 55 94 696.4 0 94 56 44 696.4 0 44 57 67 696.4 0 67 58 78 696.4 0 78 59 31 696.4 0 31 60 35 696.4 0 35 61 52 696.4 0 52 62 49 696.4 0 49 63 31 696.4 0 31 64 54 696.4 0 54 65 40 696.4 0 40 66 52 696.4 0 52 67 37 696.4 0 37 68 63 696.4 0 63 69 51 696.4 0 51 70 55 696.4 0 55 71 59 696.4 0 59 72 65 696.4 0 65 73 34 696.4 0 34 74 61 696.4 0 61 75 69 696.4 0 69 76 50 696.4 0 50 77 57 696.4 0 57 78 81 696.4 0 81 79 59 696.4 0 59 80 35 696.4 0 35 81 45 696.4 0 45 82 69 696.4 0 69 83 55 696.4 0 55 84 64 696.4 0 64 85 67 696.4 0 67 86 78 696.4 0 78 87 80 696.4 0 80 88 72 696.4 0 72 89 64 696.4 0 64 90 58 696.4 0 58 91 66 696.4 0 66 92 64 696.4 0 64 93 72 696.4 0 72 94 85 696.4 0 85 95 85 696.4 0 85 96 70 696.4 0 70 97 136 696.4 0 136 98 71 696.4 0 71 99 135 696.4 0 135 100 59 696.4 0 59 101 42 696.4 0 42 102 45 696.4 0 45 103 61 696.4 0 61 104 53 696.4 0 53 105 57 696.4 0 57 106 92 696.4 0 92 107 100 696.4 0 100 108 68 696.4 0 68 109 47 696.4 0 47 110 50 696.4 0 50 111 62 696.4 0 62 112 76 696.4 0 76 113 69 696.4 0 69 114 77 696.4 0 77 115 80 696.4 0 80 116 102 696.4 0 102 117 112 696.4 0 112 118 107 696.4 0 107 119 170 696.4 0 170 120 178 696.4 0 178 121 110 696.4 0 110 122 85 696.4 0 85 123 117 696.4 0 117 124 120 696.4 0 120 125 998 696.4 0 998 126 748 696.4 0 748 127 827 696.4 0 827 128 100 696.4 0 100 129 83 696.4 0 83 130 81 696.4 0 81 131 76 696.4 0 76 132 58 696.4 0 58 133 76 696.4 0 76 134 105 696.4 0 105 135 178 696.4 0 178 136 181 696.4 0 181 137 87 696.4 0 87 138 106 696.4 0 106 139 93 696.4 0 93 140 66 696.4 0 66 141 79 696.4 0 79 142 83 696.4 0 83 143 93 696.4 0 93 144 81 696.4 0 81 145 492 696.4 0 492 146 458 696.4 0 458 147 520 696.4 0 520 148 58 696.4 0 58 149 86 696.4 0 86 150 84 696.4 0 84 151 96 696.4 0 96 152 112 696.4 0 112 153 79 696.4 0 79 154 106 696.4 0 106 155 110 696.4 0 110 156 117 696.4 0 117 157 125 696.4 0 125 158 120 696.4 0 120 159 106 696.4 0 106 160 95 696.4 0 95 161 99 696.4 0 99 162 116 696.4 0 116 163 103 696.4 0 103 164 138 696.4 0 138 165 195 696.4 0 195 166 158 696.4 0 158 167 154 696.4 0 154 168 109 696.4 0 109 169 113 696.4 0 113 170 106 696.4 0 106 171 183 696.4 0 183 172 209 696.4 0 209 173 1026 696.4 0 1026 174 2344 696.4 0 2344 175 1350 696.4 0 1350 176 528 696.4 0 528 177 75 696.4 0 75 178 113 696.4 0 113 179 154 696.4 0 154 180 156 696.4 0 156 181 379 696.4 0 379 182 490 696.4 0 490 183 376 696.4 0 376 184 177 696.4 0 177 185 230 696.4 0 230 186 608 696.4 0 608 187 607 696.4 0 607 188 667 696.4 0 667 189 175 696.4 0 175 190 100 696.4 0 100 191 146 696.4 0 146 192 150 696.4 0 150 193 163 696.4 0 163 194 139 696.4 0 139 195 104 696.4 0 104 196 114 696.4 0 114 197 64 696.4 0 64 198 67 696.4 0 67 199 105 696.4 0 105 200 124 696.4 0 124 201 127 696.4 0 127 202 127 696.4 0 127 203 142 696.4 0 142 204 139 696.4 0 139 205 113 696.4 0 113 206 234 696.4 0 234 207 249 696.4 0 249 208 426 696.4 0 426 209 160 696.4 0 160 210 109 696.4 0 109 211 137 696.4 0 137 212 219 696.4 0 219 213 186 696.4 0 186 214 273 696.4 0 273 215 257 696.4 0 257 216 159 696.4 0 159 217 132 696.4 0 132 218 179 696.4 0 179 219 218 696.4 0 218 220 275 696.4 0 275 221 238 696.4 0 238 222 193 696.4 0 193 223 198 696.4 0 198 224 328 696.4 0 328 225 4166 696.4 0 4166 226 3245 696.4 0 3245 227 5119 696.4 0 5119 228 222 696.4 0 222 229 458 696.4 0 458 230 1566 696.4 0 1566 231 1536 696.4 0 1536 232 1498 696.4 0 1498 233 701 696.4 0 701 234 210 696.4 0 210 235 168 696.4 0 168 236 148 696.4 0 148 237 131 696.4 0 131 238 114 696.4 0 114 239 125 696.4 0 125 240 104 696.4 0 104 241 128 696.4 0 128 242 136 696.4 0 136 243 141 696.4 0 141 244 140 696.4 0 140 245 172 696.4 0 172 246 647 696.4 0 647 247 336 696.4 0 336 248 566 696.4 0 566 249 157 696.4 0 157 250 473 696.4 0 473 251 405 696.4 0 405 252 973 696.4 0 973 253 1187 696.4 0 1187 254 832 696.4 0 832 255 377 696.4 0 377 256 113 696.4 0 113 257 106 696.4 0 106 258 119 696.4 0 119 259 136 696.4 0 136 260 142 696.4 0 142 261 121 696.4 0 121 262 117 696.4 0 117 263 155 696.4 0 155 264 229 696.4 0 229 265 637 696.4 0 637 266 230 696.4 0 230 267 230 696.4 0 230 268 285 696.4 0 285 269 243 696.4 0 243 270 202 696.4 0 202 271 378 696.4 0 378 272 318 696.4 0 318 273 447 696.4 0 447 274 350 696.4 0 350 275 918 696.4 0 918 276 597 696.4 0 597 277 215 696.4 0 215 278 262 696.4 0 262 279 553 696.4 0 553 280 1738 696.4 0 1738 281 2031 696.4 0 2031 282 1160 696.4 0 1160 283 584 696.4 0 584 284 647 696.4 0 647 285 1280 696.4 0 1280 286 690 696.4 0 690 287 606 696.4 0 606 288 550 696.4 0 550 289 552 696.4 0 552 290 712 696.4 0 712 291 562 696.4 0 562 292 424 696.4 0 424 293 385 696.4 0 385 294 608 696.4 0 608 295 978 696.4 0 978 296 1536 696.4 0 1536 297 2262 696.4 0 2262 298 3368 696.4 0 3368 299 5217 696.4 0 5217 300 10111 696.4 0 10111 301 47306 696.4 0 47306 Finished in 3.15 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 70,814 Reads with adapters: 19,548 (27.6%) Reads that were too short: 41 (0.1%) Reads written (passing filters): 19,507 (27.5%) Total basepairs processed: 17,120,865 bp Total written (filtered): 5,375,037 bp (31.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 19548 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 16 124 0.0 3 0 119 5 17 18874 0.0 3 18477 386 9 2 18 40 0.0 3 1 37 2 19 23 0.0 3 2 6 15 20 382 0.0 3 355 7 0 20 21 2 0.0 3 1 1 23 1 0.0 3 0 0 0 1 29 1 0.0 3 0 1 31 1 0.0 3 1 32 1 0.0 3 0 1 36 2 0.0 3 0 0 0 2 37 2 0.0 3 1 0 0 1 38 1 0.0 3 1 39 3 0.0 3 0 0 0 3 43 1 0.0 3 0 0 0 1 46 17 0.0 3 0 0 1 16 49 11 0.0 3 0 0 2 9 55 1 0.0 3 0 0 0 1 66 16 0.0 3 0 0 0 16 72 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 4 0.0 3 0 0 0 4 113 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 123 1 0.0 3 0 1 132 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 167 3 0.0 3 0 0 0 3 177 1 0.0 3 0 0 0 1 181 3 0.0 3 0 0 1 2 182 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 201 1 0.0 3 1 206 1 0.0 3 0 1 212 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.68 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 19,507 Reads with adapters: 467 (2.4%) Reads written (passing filters): 19,507 (100.0%) Total basepairs processed: 5,375,037 bp Total written (filtered): 5,373,527 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 467 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 70.4% C: 5.8% G: 22.5% T: 1.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 359 304.8 0 359 4 107 76.2 0 107 5 1 19.0 0 1