This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.41 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 116,489 (4.1%) Reads that were too short: 25,348 (0.9%) Reads written (passing filters): 91,141 (3.2%) Total basepairs processed: 858,556,748 bp Total written (filtered): 24,961,516 bp (2.9%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 116489 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1520 696.4 0 1520 7 38306 696.4 0 38306 8 33782 696.4 0 33782 9 64 696.4 0 64 10 40 696.4 0 40 11 39 696.4 0 39 12 36 696.4 0 36 13 56 696.4 0 56 14 47 696.4 0 47 15 39 696.4 0 39 16 39 696.4 0 39 17 33 696.4 0 33 18 42 696.4 0 42 19 43 696.4 0 43 20 39 696.4 0 39 21 51 696.4 0 51 22 45 696.4 0 45 23 49 696.4 0 49 24 38 696.4 0 38 25 46 696.4 0 46 26 74 696.4 0 74 27 43 696.4 0 43 28 52 696.4 0 52 29 38 696.4 0 38 30 27 696.4 0 27 31 43 696.4 0 43 32 43 696.4 0 43 33 41 696.4 0 41 34 45 696.4 0 45 35 38 696.4 0 38 36 30 696.4 0 30 37 41 696.4 0 41 38 79 696.4 0 79 39 437 696.4 0 437 40 482 696.4 0 482 41 1415 696.4 0 1415 42 382 696.4 0 382 43 41 696.4 0 41 44 29 696.4 0 29 45 33 696.4 0 33 46 47 696.4 0 47 47 30 696.4 0 30 48 59 696.4 0 59 49 50 696.4 0 50 50 44 696.4 0 44 51 45 696.4 0 45 52 73 696.4 0 73 53 57 696.4 0 57 54 34 696.4 0 34 55 41 696.4 0 41 56 31 696.4 0 31 57 49 696.4 0 49 58 46 696.4 0 46 59 32 696.4 0 32 60 51 696.4 0 51 61 58 696.4 0 58 62 34 696.4 0 34 63 25 696.4 0 25 64 45 696.4 0 45 65 71 696.4 0 71 66 46 696.4 0 46 67 60 696.4 0 60 68 41 696.4 0 41 69 41 696.4 0 41 70 65 696.4 0 65 71 71 696.4 0 71 72 76 696.4 0 76 73 37 696.4 0 37 74 35 696.4 0 35 75 41 696.4 0 41 76 57 696.4 0 57 77 38 696.4 0 38 78 37 696.4 0 37 79 40 696.4 0 40 80 37 696.4 0 37 81 44 696.4 0 44 82 45 696.4 0 45 83 37 696.4 0 37 84 109 696.4 0 109 85 82 696.4 0 82 86 78 696.4 0 78 87 43 696.4 0 43 88 50 696.4 0 50 89 63 696.4 0 63 90 68 696.4 0 68 91 66 696.4 0 66 92 126 696.4 0 126 93 100 696.4 0 100 94 54 696.4 0 54 95 69 696.4 0 69 96 68 696.4 0 68 97 62 696.4 0 62 98 341 696.4 0 341 99 428 696.4 0 428 100 403 696.4 0 403 101 90 696.4 0 90 102 60 696.4 0 60 103 50 696.4 0 50 104 55 696.4 0 55 105 78 696.4 0 78 106 53 696.4 0 53 107 44 696.4 0 44 108 50 696.4 0 50 109 55 696.4 0 55 110 97 696.4 0 97 111 193 696.4 0 193 112 132 696.4 0 132 113 181 696.4 0 181 114 43 696.4 0 43 115 48 696.4 0 48 116 43 696.4 0 43 117 68 696.4 0 68 118 61 696.4 0 61 119 56 696.4 0 56 120 62 696.4 0 62 121 51 696.4 0 51 122 37 696.4 0 37 123 78 696.4 0 78 124 90 696.4 0 90 125 101 696.4 0 101 126 59 696.4 0 59 127 61 696.4 0 61 128 56 696.4 0 56 129 58 696.4 0 58 130 71 696.4 0 71 131 56 696.4 0 56 132 91 696.4 0 91 133 87 696.4 0 87 134 100 696.4 0 100 135 206 696.4 0 206 136 72 696.4 0 72 137 85 696.4 0 85 138 90 696.4 0 90 139 81 696.4 0 81 140 51 696.4 0 51 141 62 696.4 0 62 142 63 696.4 0 63 143 122 696.4 0 122 144 82 696.4 0 82 145 73 696.4 0 73 146 129 696.4 0 129 147 75 696.4 0 75 148 58 696.4 0 58 149 64 696.4 0 64 150 61 696.4 0 61 151 62 696.4 0 62 152 68 696.4 0 68 153 83 696.4 0 83 154 60 696.4 0 60 155 60 696.4 0 60 156 77 696.4 0 77 157 85 696.4 0 85 158 305 696.4 0 305 159 315 696.4 0 315 160 166 696.4 0 166 161 55 696.4 0 55 162 83 696.4 0 83 163 85 696.4 0 85 164 73 696.4 0 73 165 54 696.4 0 54 166 54 696.4 0 54 167 72 696.4 0 72 168 74 696.4 0 74 169 105 696.4 0 105 170 127 696.4 0 127 171 183 696.4 0 183 172 255 696.4 0 255 173 67 696.4 0 67 174 52 696.4 0 52 175 51 696.4 0 51 176 80 696.4 0 80 177 163 696.4 0 163 178 238 696.4 0 238 179 189 696.4 0 189 180 120 696.4 0 120 181 77 696.4 0 77 182 65 696.4 0 65 183 146 696.4 0 146 184 159 696.4 0 159 185 208 696.4 0 208 186 96 696.4 0 96 187 92 696.4 0 92 188 134 696.4 0 134 189 173 696.4 0 173 190 96 696.4 0 96 191 85 696.4 0 85 192 70 696.4 0 70 193 56 696.4 0 56 194 72 696.4 0 72 195 92 696.4 0 92 196 84 696.4 0 84 197 62 696.4 0 62 198 75 696.4 0 75 199 64 696.4 0 64 200 64 696.4 0 64 201 74 696.4 0 74 202 79 696.4 0 79 203 93 696.4 0 93 204 89 696.4 0 89 205 63 696.4 0 63 206 76 696.4 0 76 207 85 696.4 0 85 208 71 696.4 0 71 209 75 696.4 0 75 210 167 696.4 0 167 211 213 696.4 0 213 212 140 696.4 0 140 213 150 696.4 0 150 214 604 696.4 0 604 215 736 696.4 0 736 216 1018 696.4 0 1018 217 147 696.4 0 147 218 57 696.4 0 57 219 62 696.4 0 62 220 68 696.4 0 68 221 78 696.4 0 78 222 92 696.4 0 92 223 557 696.4 0 557 224 992 696.4 0 992 225 467 696.4 0 467 226 112 696.4 0 112 227 147 696.4 0 147 228 89 696.4 0 89 229 72 696.4 0 72 230 57 696.4 0 57 231 62 696.4 0 62 232 63 696.4 0 63 233 76 696.4 0 76 234 79 696.4 0 79 235 85 696.4 0 85 236 72 696.4 0 72 237 82 696.4 0 82 238 134 696.4 0 134 239 155 696.4 0 155 240 118 696.4 0 118 241 114 696.4 0 114 242 122 696.4 0 122 243 109 696.4 0 109 244 125 696.4 0 125 245 95 696.4 0 95 246 86 696.4 0 86 247 75 696.4 0 75 248 64 696.4 0 64 249 75 696.4 0 75 250 70 696.4 0 70 251 85 696.4 0 85 252 79 696.4 0 79 253 97 696.4 0 97 254 75 696.4 0 75 255 84 696.4 0 84 256 208 696.4 0 208 257 254 696.4 0 254 258 297 696.4 0 297 259 216 696.4 0 216 260 105 696.4 0 105 261 106 696.4 0 106 262 119 696.4 0 119 263 648 696.4 0 648 264 340 696.4 0 340 265 327 696.4 0 327 266 86 696.4 0 86 267 67 696.4 0 67 268 103 696.4 0 103 269 100 696.4 0 100 270 181 696.4 0 181 271 413 696.4 0 413 272 3210 696.4 0 3210 273 2608 696.4 0 2608 274 1725 696.4 0 1725 275 148 696.4 0 148 276 275 696.4 0 275 277 285 696.4 0 285 278 379 696.4 0 379 279 116 696.4 0 116 280 87 696.4 0 87 281 66 696.4 0 66 282 140 696.4 0 140 283 262 696.4 0 262 284 417 696.4 0 417 285 427 696.4 0 427 286 297 696.4 0 297 287 134 696.4 0 134 288 122 696.4 0 122 289 126 696.4 0 126 290 69 696.4 0 69 291 71 696.4 0 71 292 67 696.4 0 67 293 79 696.4 0 79 294 74 696.4 0 74 295 78 696.4 0 78 296 63 696.4 0 63 297 123 696.4 0 123 298 266 696.4 0 266 299 417 696.4 0 417 300 373 696.4 0 373 301 337 696.4 0 337 Finished in 4.90 s (54 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 91,141 Reads with adapters: 35,059 (38.5%) Reads that were too short: 50 (0.1%) Reads written (passing filters): 35,009 (38.4%) Total basepairs processed: 24,961,516 bp Total written (filtered): 9,517,964 bp (38.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 35059 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 0 4 18 9 0.0 3 0 0 7 0 2 19 458 0.0 3 0 370 35 35 18 20 33955 0.0 4 31805 1709 343 72 26 21 100 0.0 4 19 74 5 1 1 22 7 0.0 4 2 1 4 23 91 0.0 4 1 2 0 62 26 24 2 0.0 4 0 1 0 0 1 27 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 1 36 15 0.0 4 0 0 0 0 15 45 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 1 91 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 126 4 0.0 4 0 0 0 0 4 127 355 0.0 4 0 0 0 0 355 152 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 2 0.0 4 0 0 0 0 2 218 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 1 3 229 4 0.0 4 0 0 0 1 3 233 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 241 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 263 3 0.0 4 0 0 0 0 3 264 4 0.0 4 0 0 0 0 4 265 2 0.0 4 0 0 0 0 2 275 1 0.0 4 0 0 0 0 1 276 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 292 2 0.0 4 0 0 0 0 2 293 2 0.0 4 0 0 0 0 2 294 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.28 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 35,009 Reads with adapters: 7,085 (20.2%) Reads written (passing filters): 35,009 (100.0%) Total basepairs processed: 9,517,964 bp Total written (filtered): 9,442,361 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7085 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.8% C: 9.4% G: 85.5% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 39 547.0 0 39 4 6 136.8 0 6 5 1 34.2 0 1 7 1 2.1 0 1 8 1 0.5 0 1 9 118 0.1 0 7 111 10 3026 0.0 1 257 2769 11 3814 0.0 1 171 3643 12 56 0.0 1 3 53 13 10 0.0 1 0 10 14 5 0.0 1 0 5 15 1 0.0 1 0 1 141 1 0.0 1 0 1 151 2 0.0 1 2 209 4 0.0 1 3 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.