This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.71 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 116,648 (4.1%) Reads that were too short: 20,289 (0.7%) Reads written (passing filters): 96,359 (3.4%) Total basepairs processed: 858,556,748 bp Total written (filtered): 26,401,399 bp (3.1%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 116648 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1190 696.4 0 1190 7 34985 696.4 0 34985 8 40263 696.4 0 40263 9 86 696.4 0 86 10 59 696.4 0 59 11 55 696.4 0 55 12 54 696.4 0 54 13 51 696.4 0 51 14 57 696.4 0 57 15 56 696.4 0 56 16 45 696.4 0 45 17 42 696.4 0 42 18 57 696.4 0 57 19 52 696.4 0 52 20 61 696.4 0 61 21 80 696.4 0 80 22 72 696.4 0 72 23 63 696.4 0 63 24 42 696.4 0 42 25 45 696.4 0 45 26 64 696.4 0 64 27 56 696.4 0 56 28 63 696.4 0 63 29 67 696.4 0 67 30 52 696.4 0 52 31 59 696.4 0 59 32 67 696.4 0 67 33 56 696.4 0 56 34 60 696.4 0 60 35 67 696.4 0 67 36 68 696.4 0 68 37 44 696.4 0 44 38 79 696.4 0 79 39 568 696.4 0 568 40 487 696.4 0 487 41 1173 696.4 0 1173 42 382 696.4 0 382 43 57 696.4 0 57 44 47 696.4 0 47 45 43 696.4 0 43 46 57 696.4 0 57 47 50 696.4 0 50 48 34 696.4 0 34 49 70 696.4 0 70 50 54 696.4 0 54 51 52 696.4 0 52 52 56 696.4 0 56 53 62 696.4 0 62 54 50 696.4 0 50 55 43 696.4 0 43 56 36 696.4 0 36 57 58 696.4 0 58 58 56 696.4 0 56 59 51 696.4 0 51 60 79 696.4 0 79 61 47 696.4 0 47 62 45 696.4 0 45 63 42 696.4 0 42 64 63 696.4 0 63 65 1814 696.4 0 1814 66 201 696.4 0 201 67 70 696.4 0 70 68 64 696.4 0 64 69 49 696.4 0 49 70 81 696.4 0 81 71 102 696.4 0 102 72 88 696.4 0 88 73 55 696.4 0 55 74 53 696.4 0 53 75 52 696.4 0 52 76 71 696.4 0 71 77 47 696.4 0 47 78 71 696.4 0 71 79 61 696.4 0 61 80 54 696.4 0 54 81 68 696.4 0 68 82 52 696.4 0 52 83 76 696.4 0 76 84 76 696.4 0 76 85 69 696.4 0 69 86 72 696.4 0 72 87 62 696.4 0 62 88 53 696.4 0 53 89 44 696.4 0 44 90 72 696.4 0 72 91 108 696.4 0 108 92 105 696.4 0 105 93 109 696.4 0 109 94 58 696.4 0 58 95 83 696.4 0 83 96 85 696.4 0 85 97 73 696.4 0 73 98 231 696.4 0 231 99 347 696.4 0 347 100 348 696.4 0 348 101 95 696.4 0 95 102 91 696.4 0 91 103 68 696.4 0 68 104 60 696.4 0 60 105 82 696.4 0 82 106 64 696.4 0 64 107 64 696.4 0 64 108 71 696.4 0 71 109 65 696.4 0 65 110 104 696.4 0 104 111 135 696.4 0 135 112 138 696.4 0 138 113 113 696.4 0 113 114 61 696.4 0 61 115 75 696.4 0 75 116 51 696.4 0 51 117 70 696.4 0 70 118 67 696.4 0 67 119 65 696.4 0 65 120 81 696.4 0 81 121 49 696.4 0 49 122 55 696.4 0 55 123 79 696.4 0 79 124 101 696.4 0 101 125 117 696.4 0 117 126 63 696.4 0 63 127 63 696.4 0 63 128 62 696.4 0 62 129 54 696.4 0 54 130 53 696.4 0 53 131 88 696.4 0 88 132 105 696.4 0 105 133 126 696.4 0 126 134 89 696.4 0 89 135 217 696.4 0 217 136 59 696.4 0 59 137 92 696.4 0 92 138 86 696.4 0 86 139 96 696.4 0 96 140 59 696.4 0 59 141 75 696.4 0 75 142 66 696.4 0 66 143 103 696.4 0 103 144 81 696.4 0 81 145 65 696.4 0 65 146 139 696.4 0 139 147 82 696.4 0 82 148 72 696.4 0 72 149 59 696.4 0 59 150 75 696.4 0 75 151 73 696.4 0 73 152 66 696.4 0 66 153 75 696.4 0 75 154 75 696.4 0 75 155 71 696.4 0 71 156 73 696.4 0 73 157 81 696.4 0 81 158 235 696.4 0 235 159 292 696.4 0 292 160 171 696.4 0 171 161 71 696.4 0 71 162 54 696.4 0 54 163 68 696.4 0 68 164 78 696.4 0 78 165 73 696.4 0 73 166 56 696.4 0 56 167 82 696.4 0 82 168 87 696.4 0 87 169 89 696.4 0 89 170 135 696.4 0 135 171 156 696.4 0 156 172 291 696.4 0 291 173 91 696.4 0 91 174 55 696.4 0 55 175 50 696.4 0 50 176 93 696.4 0 93 177 98 696.4 0 98 178 126 696.4 0 126 179 121 696.4 0 121 180 91 696.4 0 91 181 68 696.4 0 68 182 69 696.4 0 69 183 139 696.4 0 139 184 157 696.4 0 157 185 202 696.4 0 202 186 91 696.4 0 91 187 88 696.4 0 88 188 94 696.4 0 94 189 122 696.4 0 122 190 82 696.4 0 82 191 75 696.4 0 75 192 81 696.4 0 81 193 54 696.4 0 54 194 60 696.4 0 60 195 82 696.4 0 82 196 92 696.4 0 92 197 86 696.4 0 86 198 83 696.4 0 83 199 67 696.4 0 67 200 71 696.4 0 71 201 66 696.4 0 66 202 70 696.4 0 70 203 69 696.4 0 69 204 84 696.4 0 84 205 65 696.4 0 65 206 73 696.4 0 73 207 85 696.4 0 85 208 63 696.4 0 63 209 67 696.4 0 67 210 116 696.4 0 116 211 130 696.4 0 130 212 99 696.4 0 99 213 95 696.4 0 95 214 243 696.4 0 243 215 389 696.4 0 389 216 431 696.4 0 431 217 101 696.4 0 101 218 56 696.4 0 56 219 64 696.4 0 64 220 66 696.4 0 66 221 78 696.4 0 78 222 107 696.4 0 107 223 546 696.4 0 546 224 976 696.4 0 976 225 430 696.4 0 430 226 74 696.4 0 74 227 152 696.4 0 152 228 56 696.4 0 56 229 68 696.4 0 68 230 64 696.4 0 64 231 47 696.4 0 47 232 55 696.4 0 55 233 69 696.4 0 69 234 55 696.4 0 55 235 62 696.4 0 62 236 77 696.4 0 77 237 75 696.4 0 75 238 116 696.4 0 116 239 113 696.4 0 113 240 89 696.4 0 89 241 69 696.4 0 69 242 81 696.4 0 81 243 70 696.4 0 70 244 89 696.4 0 89 245 85 696.4 0 85 246 67 696.4 0 67 247 61 696.4 0 61 248 65 696.4 0 65 249 80 696.4 0 80 250 73 696.4 0 73 251 80 696.4 0 80 252 80 696.4 0 80 253 54 696.4 0 54 254 70 696.4 0 70 255 62 696.4 0 62 256 96 696.4 0 96 257 104 696.4 0 104 258 88 696.4 0 88 259 123 696.4 0 123 260 70 696.4 0 70 261 83 696.4 0 83 262 97 696.4 0 97 263 850 696.4 0 850 264 383 696.4 0 383 265 336 696.4 0 336 266 73 696.4 0 73 267 71 696.4 0 71 268 76 696.4 0 76 269 85 696.4 0 85 270 133 696.4 0 133 271 296 696.4 0 296 272 3003 696.4 0 3003 273 2509 696.4 0 2509 274 1660 696.4 0 1660 275 87 696.4 0 87 276 98 696.4 0 98 277 127 696.4 0 127 278 144 696.4 0 144 279 92 696.4 0 92 280 65 696.4 0 65 281 68 696.4 0 68 282 94 696.4 0 94 283 171 696.4 0 171 284 251 696.4 0 251 285 292 696.4 0 292 286 182 696.4 0 182 287 86 696.4 0 86 288 106 696.4 0 106 289 104 696.4 0 104 290 52 696.4 0 52 291 75 696.4 0 75 292 53 696.4 0 53 293 50 696.4 0 50 294 65 696.4 0 65 295 61 696.4 0 61 296 64 696.4 0 64 297 88 696.4 0 88 298 185 696.4 0 185 299 295 696.4 0 295 300 292 696.4 0 292 301 145 696.4 0 145 Finished in 4.47 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 96,359 Reads with adapters: 35,097 (36.4%) Reads that were too short: 33 (0.0%) Reads written (passing filters): 35,064 (36.4%) Total basepairs processed: 26,401,399 bp Total written (filtered): 9,702,421 bp (36.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 35097 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 162 0.0 3 0 157 5 17 34714 0.0 3 33917 770 24 3 18 78 0.0 3 11 66 1 19 20 0.0 3 1 0 16 3 20 10 0.0 3 0 0 0 10 21 1 0.0 3 1 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 73 4 0.0 3 0 0 0 4 74 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 83 5 0.0 3 0 0 0 5 98 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 1 106 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 1 120 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 132 20 0.0 3 0 0 0 20 133 10 0.0 3 0 0 0 10 135 13 0.0 3 0 0 1 12 136 2 0.0 3 0 0 0 2 137 2 0.0 3 0 0 0 2 147 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 163 1 0.0 3 0 0 0 1 165 1 0.0 3 0 1 178 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 217 4 0.0 3 0 0 0 4 221 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 244 2 0.0 3 0 0 0 2 245 4 0.0 3 0 0 0 4 246 1 0.0 3 0 0 0 1 247 2 0.0 3 0 0 0 2 248 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.23 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 35,064 Reads with adapters: 98 (0.3%) Reads written (passing filters): 35,064 (100.0%) Total basepairs processed: 9,702,421 bp Total written (filtered): 9,702,083 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 98 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 36.7% C: 40.8% G: 15.3% T: 7.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 68 547.9 0 68 4 26 137.0 0 26 6 3 8.6 0 3 12 1 0.0 1 0 1