This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.43 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 202,824 (7.1%) Reads that were too short: 27,871 (1.0%) Reads written (passing filters): 174,953 (6.1%) Total basepairs processed: 858,556,748 bp Total written (filtered): 37,628,551 bp (4.4%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 202824 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3335 696.4 0 3335 7 27963 696.4 0 27963 8 1193 696.4 0 1193 9 30571 696.4 0 30571 10 182 696.4 0 182 11 151 696.4 0 151 12 96 696.4 0 96 13 108 696.4 0 108 14 117 696.4 0 117 15 97 696.4 0 97 16 86 696.4 0 86 17 116 696.4 0 116 18 129 696.4 0 129 19 118 696.4 0 118 20 152 696.4 0 152 21 321 696.4 0 321 22 342 696.4 0 342 23 436 696.4 0 436 24 866 696.4 0 866 25 1554 696.4 0 1554 26 1996 696.4 0 1996 27 114 696.4 0 114 28 83 696.4 0 83 29 77 696.4 0 77 30 75 696.4 0 75 31 80 696.4 0 80 32 98 696.4 0 98 33 113 696.4 0 113 34 77 696.4 0 77 35 83 696.4 0 83 36 108 696.4 0 108 37 98 696.4 0 98 38 64 696.4 0 64 39 69 696.4 0 69 40 94 696.4 0 94 41 106 696.4 0 106 42 85 696.4 0 85 43 63 696.4 0 63 44 71 696.4 0 71 45 109 696.4 0 109 46 102 696.4 0 102 47 93 696.4 0 93 48 114 696.4 0 114 49 98 696.4 0 98 50 94 696.4 0 94 51 93 696.4 0 93 52 94 696.4 0 94 53 103 696.4 0 103 54 127 696.4 0 127 55 127 696.4 0 127 56 125 696.4 0 125 57 118 696.4 0 118 58 99 696.4 0 99 59 114 696.4 0 114 60 128 696.4 0 128 61 883 696.4 0 883 62 668 696.4 0 668 63 737 696.4 0 737 64 90 696.4 0 90 65 98 696.4 0 98 66 111 696.4 0 111 67 87 696.4 0 87 68 115 696.4 0 115 69 264 696.4 0 264 70 324 696.4 0 324 71 404 696.4 0 404 72 160 696.4 0 160 73 283 696.4 0 283 74 140 696.4 0 140 75 136 696.4 0 136 76 149 696.4 0 149 77 128 696.4 0 128 78 155 696.4 0 155 79 179 696.4 0 179 80 304 696.4 0 304 81 173 696.4 0 173 82 441 696.4 0 441 83 2468 696.4 0 2468 84 1694 696.4 0 1694 85 1657 696.4 0 1657 86 161 696.4 0 161 87 102 696.4 0 102 88 114 696.4 0 114 89 223 696.4 0 223 90 138 696.4 0 138 91 86 696.4 0 86 92 150 696.4 0 150 93 105 696.4 0 105 94 82 696.4 0 82 95 139 696.4 0 139 96 122 696.4 0 122 97 136 696.4 0 136 98 160 696.4 0 160 99 214 696.4 0 214 100 103 696.4 0 103 101 154 696.4 0 154 102 198 696.4 0 198 103 136 696.4 0 136 104 129 696.4 0 129 105 217 696.4 0 217 106 225 696.4 0 225 107 230 696.4 0 230 108 153 696.4 0 153 109 211 696.4 0 211 110 305 696.4 0 305 111 497 696.4 0 497 112 6264 696.4 0 6264 113 3778 696.4 0 3778 114 6136 696.4 0 6136 115 241 696.4 0 241 116 142 696.4 0 142 117 195 696.4 0 195 118 259 696.4 0 259 119 423 696.4 0 423 120 4535 696.4 0 4535 121 2277 696.4 0 2277 122 2576 696.4 0 2576 123 252 696.4 0 252 124 258 696.4 0 258 125 760 696.4 0 760 126 248 696.4 0 248 127 319 696.4 0 319 128 146 696.4 0 146 129 137 696.4 0 137 130 221 696.4 0 221 131 162 696.4 0 162 132 160 696.4 0 160 133 152 696.4 0 152 134 233 696.4 0 233 135 162 696.4 0 162 136 185 696.4 0 185 137 177 696.4 0 177 138 234 696.4 0 234 139 206 696.4 0 206 140 183 696.4 0 183 141 271 696.4 0 271 142 656 696.4 0 656 143 480 696.4 0 480 144 668 696.4 0 668 145 328 696.4 0 328 146 547 696.4 0 547 147 3346 696.4 0 3346 148 3384 696.4 0 3384 149 3689 696.4 0 3689 150 2624 696.4 0 2624 151 545 696.4 0 545 152 594 696.4 0 594 153 291 696.4 0 291 154 188 696.4 0 188 155 236 696.4 0 236 156 181 696.4 0 181 157 345 696.4 0 345 158 217 696.4 0 217 159 185 696.4 0 185 160 189 696.4 0 189 161 262 696.4 0 262 162 242 696.4 0 242 163 442 696.4 0 442 164 402 696.4 0 402 165 748 696.4 0 748 166 1399 696.4 0 1399 167 1277 696.4 0 1277 168 1194 696.4 0 1194 169 802 696.4 0 802 170 582 696.4 0 582 171 2533 696.4 0 2533 172 2032 696.4 0 2032 173 2749 696.4 0 2749 174 937 696.4 0 937 175 385 696.4 0 385 176 311 696.4 0 311 177 246 696.4 0 246 178 204 696.4 0 204 179 167 696.4 0 167 180 173 696.4 0 173 181 234 696.4 0 234 182 305 696.4 0 305 183 1783 696.4 0 1783 184 1239 696.4 0 1239 185 742 696.4 0 742 186 417 696.4 0 417 187 472 696.4 0 472 188 472 696.4 0 472 189 528 696.4 0 528 190 460 696.4 0 460 191 2338 696.4 0 2338 192 2644 696.4 0 2644 193 3216 696.4 0 3216 194 1303 696.4 0 1303 195 357 696.4 0 357 196 326 696.4 0 326 197 237 696.4 0 237 198 189 696.4 0 189 199 259 696.4 0 259 200 427 696.4 0 427 201 370 696.4 0 370 202 282 696.4 0 282 203 178 696.4 0 178 204 136 696.4 0 136 205 166 696.4 0 166 206 189 696.4 0 189 207 268 696.4 0 268 208 234 696.4 0 234 209 192 696.4 0 192 210 150 696.4 0 150 211 129 696.4 0 129 212 150 696.4 0 150 213 161 696.4 0 161 214 159 696.4 0 159 215 336 696.4 0 336 216 260 696.4 0 260 217 231 696.4 0 231 218 308 696.4 0 308 219 207 696.4 0 207 220 213 696.4 0 213 221 116 696.4 0 116 222 139 696.4 0 139 223 150 696.4 0 150 224 142 696.4 0 142 225 259 696.4 0 259 226 718 696.4 0 718 227 432 696.4 0 432 228 573 696.4 0 573 229 441 696.4 0 441 230 339 696.4 0 339 231 132 696.4 0 132 232 143 696.4 0 143 233 156 696.4 0 156 234 126 696.4 0 126 235 144 696.4 0 144 236 119 696.4 0 119 237 162 696.4 0 162 238 136 696.4 0 136 239 156 696.4 0 156 240 145 696.4 0 145 241 128 696.4 0 128 242 144 696.4 0 144 243 190 696.4 0 190 244 153 696.4 0 153 245 248 696.4 0 248 246 408 696.4 0 408 247 343 696.4 0 343 248 213 696.4 0 213 249 123 696.4 0 123 250 134 696.4 0 134 251 382 696.4 0 382 252 267 696.4 0 267 253 299 696.4 0 299 254 212 696.4 0 212 255 200 696.4 0 200 256 242 696.4 0 242 257 266 696.4 0 266 258 170 696.4 0 170 259 176 696.4 0 176 260 139 696.4 0 139 261 159 696.4 0 159 262 191 696.4 0 191 263 149 696.4 0 149 264 198 696.4 0 198 265 224 696.4 0 224 266 364 696.4 0 364 267 3403 696.4 0 3403 268 1681 696.4 0 1681 269 1700 696.4 0 1700 270 191 696.4 0 191 271 133 696.4 0 133 272 117 696.4 0 117 273 137 696.4 0 137 274 144 696.4 0 144 275 192 696.4 0 192 276 165 696.4 0 165 277 451 696.4 0 451 278 253 696.4 0 253 279 365 696.4 0 365 280 520 696.4 0 520 281 227 696.4 0 227 282 653 696.4 0 653 283 161 696.4 0 161 284 119 696.4 0 119 285 110 696.4 0 110 286 146 696.4 0 146 287 124 696.4 0 124 288 154 696.4 0 154 289 176 696.4 0 176 290 147 696.4 0 147 291 117 696.4 0 117 292 148 696.4 0 148 293 164 696.4 0 164 294 188 696.4 0 188 295 167 696.4 0 167 296 166 696.4 0 166 297 215 696.4 0 215 298 358 696.4 0 358 299 235 696.4 0 235 300 138 696.4 0 138 301 107 696.4 0 107 Finished in 8.00 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 174,953 Reads with adapters: 29,819 (17.0%) Reads that were too short: 139 (0.1%) Reads written (passing filters): 29,680 (17.0%) Total basepairs processed: 37,628,551 bp Total written (filtered): 7,991,922 bp (21.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 29819 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 1 2 18 7 0.0 3 0 0 4 2 1 19 288 0.0 3 0 240 26 15 7 20 27962 0.0 4 26706 1030 168 47 11 21 77 0.0 4 13 55 6 1 2 22 13 0.0 4 1 0 4 1 7 23 39 0.0 4 1 1 1 24 12 24 2 0.0 4 0 0 0 0 2 25 2 0.0 4 0 0 0 0 2 26 1 0.0 4 0 0 0 1 27 1 0.0 4 0 0 0 0 1 28 1 0.0 4 0 0 0 0 1 33 2 0.0 4 0 0 0 0 2 34 1 0.0 4 0 0 0 0 1 35 2 0.0 4 0 0 0 0 2 39 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 49 4 0.0 4 0 0 0 1 3 50 4 0.0 4 0 0 0 0 4 51 95 0.0 4 0 0 0 0 95 54 3 0.0 4 0 0 0 0 3 59 1 0.0 4 0 0 0 0 1 60 2 0.0 4 0 0 0 0 2 65 1 0.0 4 0 0 0 0 1 69 2 0.0 4 0 0 0 0 2 70 3 0.0 4 0 0 0 0 3 71 2 0.0 4 0 0 0 0 2 72 1 0.0 4 0 0 0 0 1 73 4 0.0 4 0 0 0 0 4 74 9 0.0 4 0 0 0 0 9 75 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 1 86 2 0.0 4 0 0 0 0 2 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 97 2 0.0 4 0 0 0 2 98 2 0.0 4 0 0 0 0 2 99 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 110 37 0.0 4 0 0 0 0 37 111 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 113 4 0.0 4 0 0 0 0 4 116 1 0.0 4 0 0 0 0 1 117 2 0.0 4 0 0 0 0 2 123 2 0.0 4 0 0 0 0 2 124 1 0.0 4 0 0 0 0 1 126 12 0.0 4 0 0 0 0 12 127 1104 0.0 4 0 0 0 0 1104 128 9 0.0 4 0 0 0 0 9 129 1 0.0 4 0 0 0 0 1 130 15 0.0 4 0 0 0 0 15 131 1 0.0 4 0 0 1 136 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 147 2 0.0 4 0 0 0 0 2 149 2 0.0 4 0 0 0 0 2 150 2 0.0 4 0 0 0 0 2 152 2 0.0 4 0 0 0 0 2 153 1 0.0 4 0 0 0 0 1 158 2 0.0 4 0 0 0 0 2 160 2 0.0 4 0 0 0 0 2 166 2 0.0 4 0 0 0 0 2 167 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 177 2 0.0 4 0 0 0 0 2 179 2 0.0 4 0 0 0 0 2 181 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 7 0.0 4 0 0 0 0 7 209 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 211 2 0.0 4 0 0 0 0 2 212 1 0.0 4 0 0 0 0 1 214 2 0.0 4 0 0 0 0 2 217 2 0.0 4 0 0 0 0 2 220 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 4 0.0 4 0 0 0 0 4 230 1 0.0 4 0 0 0 0 1 232 2 0.0 4 0 0 0 0 2 235 2 0.0 4 0 0 0 0 2 246 1 0.0 4 0 0 0 0 1 249 1 0.0 4 0 0 0 0 1 250 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 2 0.0 4 0 0 0 0 2 265 1 0.0 4 0 0 0 1 274 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.03 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 29,680 Reads with adapters: 2,831 (9.5%) Reads written (passing filters): 29,680 (100.0%) Total basepairs processed: 7,991,922 bp Total written (filtered): 7,958,697 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2831 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.1% C: 10.7% G: 82.1% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 103 463.8 0 103 4 10 115.9 0 10 9 6 0.1 0 1 5 10 56 0.0 1 5 51 11 2233 0.0 1 136 2097 12 394 0.0 1 14 380 13 8 0.0 1 0 8 14 4 0.0 1 0 4 15 2 0.0 1 0 2 16 1 0.0 1 0 1 152 3 0.0 1 3 208 1 0.0 1 1 210 9 0.0 1 8 1 211 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.