This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.40 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 174,309 (6.1%) Reads that were too short: 25,958 (0.9%) Reads written (passing filters): 148,351 (5.2%) Total basepairs processed: 858,556,748 bp Total written (filtered): 33,375,653 bp (3.9%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 174309 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2756 696.4 0 2756 7 33564 696.4 0 33564 8 748 696.4 0 748 9 27681 696.4 0 27681 10 126 696.4 0 126 11 119 696.4 0 119 12 108 696.4 0 108 13 113 696.4 0 113 14 114 696.4 0 114 15 100 696.4 0 100 16 112 696.4 0 112 17 129 696.4 0 129 18 113 696.4 0 113 19 117 696.4 0 117 20 111 696.4 0 111 21 186 696.4 0 186 22 178 696.4 0 178 23 172 696.4 0 172 24 311 696.4 0 311 25 467 696.4 0 467 26 841 696.4 0 841 27 106 696.4 0 106 28 118 696.4 0 118 29 115 696.4 0 115 30 92 696.4 0 92 31 76 696.4 0 76 32 125 696.4 0 125 33 110 696.4 0 110 34 107 696.4 0 107 35 106 696.4 0 106 36 120 696.4 0 120 37 87 696.4 0 87 38 119 696.4 0 119 39 116 696.4 0 116 40 114 696.4 0 114 41 126 696.4 0 126 42 92 696.4 0 92 43 109 696.4 0 109 44 120 696.4 0 120 45 102 696.4 0 102 46 128 696.4 0 128 47 106 696.4 0 106 48 100 696.4 0 100 49 114 696.4 0 114 50 96 696.4 0 96 51 204 696.4 0 204 52 101 696.4 0 101 53 115 696.4 0 115 54 127 696.4 0 127 55 97 696.4 0 97 56 109 696.4 0 109 57 104 696.4 0 104 58 129 696.4 0 129 59 147 696.4 0 147 60 126 696.4 0 126 61 530 696.4 0 530 62 354 696.4 0 354 63 303 696.4 0 303 64 102 696.4 0 102 65 136 696.4 0 136 66 131 696.4 0 131 67 116 696.4 0 116 68 99 696.4 0 99 69 238 696.4 0 238 70 219 696.4 0 219 71 185 696.4 0 185 72 117 696.4 0 117 73 93 696.4 0 93 74 108 696.4 0 108 75 113 696.4 0 113 76 142 696.4 0 142 77 166 696.4 0 166 78 177 696.4 0 177 79 169 696.4 0 169 80 211 696.4 0 211 81 171 696.4 0 171 82 251 696.4 0 251 83 1054 696.4 0 1054 84 677 696.4 0 677 85 802 696.4 0 802 86 140 696.4 0 140 87 155 696.4 0 155 88 168 696.4 0 168 89 150 696.4 0 150 90 151 696.4 0 151 91 136 696.4 0 136 92 155 696.4 0 155 93 127 696.4 0 127 94 104 696.4 0 104 95 136 696.4 0 136 96 140 696.4 0 140 97 148 696.4 0 148 98 186 696.4 0 186 99 276 696.4 0 276 100 144 696.4 0 144 101 172 696.4 0 172 102 253 696.4 0 253 103 144 696.4 0 144 104 149 696.4 0 149 105 201 696.4 0 201 106 187 696.4 0 187 107 256 696.4 0 256 108 188 696.4 0 188 109 216 696.4 0 216 110 295 696.4 0 295 111 410 696.4 0 410 112 5966 696.4 0 5966 113 3738 696.4 0 3738 114 6267 696.4 0 6267 115 210 696.4 0 210 116 169 696.4 0 169 117 173 696.4 0 173 118 240 696.4 0 240 119 313 696.4 0 313 120 4767 696.4 0 4767 121 2317 696.4 0 2317 122 1931 696.4 0 1931 123 186 696.4 0 186 124 226 696.4 0 226 125 613 696.4 0 613 126 310 696.4 0 310 127 341 696.4 0 341 128 135 696.4 0 135 129 154 696.4 0 154 130 179 696.4 0 179 131 152 696.4 0 152 132 140 696.4 0 140 133 126 696.4 0 126 134 170 696.4 0 170 135 136 696.4 0 136 136 148 696.4 0 148 137 156 696.4 0 156 138 194 696.4 0 194 139 169 696.4 0 169 140 186 696.4 0 186 141 175 696.4 0 175 142 433 696.4 0 433 143 368 696.4 0 368 144 416 696.4 0 416 145 192 696.4 0 192 146 231 696.4 0 231 147 1287 696.4 0 1287 148 1355 696.4 0 1355 149 2114 696.4 0 2114 150 1146 696.4 0 1146 151 270 696.4 0 270 152 331 696.4 0 331 153 177 696.4 0 177 154 160 696.4 0 160 155 186 696.4 0 186 156 152 696.4 0 152 157 223 696.4 0 223 158 192 696.4 0 192 159 158 696.4 0 158 160 156 696.4 0 156 161 185 696.4 0 185 162 193 696.4 0 193 163 199 696.4 0 199 164 202 696.4 0 202 165 326 696.4 0 326 166 750 696.4 0 750 167 619 696.4 0 619 168 678 696.4 0 678 169 430 696.4 0 430 170 355 696.4 0 355 171 1582 696.4 0 1582 172 1680 696.4 0 1680 173 1958 696.4 0 1958 174 682 696.4 0 682 175 304 696.4 0 304 176 254 696.4 0 254 177 258 696.4 0 258 178 180 696.4 0 180 179 166 696.4 0 166 180 185 696.4 0 185 181 203 696.4 0 203 182 230 696.4 0 230 183 2416 696.4 0 2416 184 1456 696.4 0 1456 185 861 696.4 0 861 186 276 696.4 0 276 187 274 696.4 0 274 188 304 696.4 0 304 189 293 696.4 0 293 190 269 696.4 0 269 191 1287 696.4 0 1287 192 1744 696.4 0 1744 193 1853 696.4 0 1853 194 765 696.4 0 765 195 219 696.4 0 219 196 296 696.4 0 296 197 209 696.4 0 209 198 205 696.4 0 205 199 274 696.4 0 274 200 424 696.4 0 424 201 293 696.4 0 293 202 228 696.4 0 228 203 177 696.4 0 177 204 132 696.4 0 132 205 142 696.4 0 142 206 160 696.4 0 160 207 177 696.4 0 177 208 172 696.4 0 172 209 168 696.4 0 168 210 157 696.4 0 157 211 131 696.4 0 131 212 131 696.4 0 131 213 155 696.4 0 155 214 138 696.4 0 138 215 352 696.4 0 352 216 275 696.4 0 275 217 231 696.4 0 231 218 227 696.4 0 227 219 194 696.4 0 194 220 163 696.4 0 163 221 144 696.4 0 144 222 120 696.4 0 120 223 125 696.4 0 125 224 143 696.4 0 143 225 199 696.4 0 199 226 488 696.4 0 488 227 336 696.4 0 336 228 492 696.4 0 492 229 366 696.4 0 366 230 326 696.4 0 326 231 116 696.4 0 116 232 129 696.4 0 129 233 139 696.4 0 139 234 148 696.4 0 148 235 147 696.4 0 147 236 135 696.4 0 135 237 186 696.4 0 186 238 148 696.4 0 148 239 135 696.4 0 135 240 148 696.4 0 148 241 149 696.4 0 149 242 124 696.4 0 124 243 163 696.4 0 163 244 149 696.4 0 149 245 194 696.4 0 194 246 187 696.4 0 187 247 202 696.4 0 202 248 123 696.4 0 123 249 117 696.4 0 117 250 134 696.4 0 134 251 299 696.4 0 299 252 228 696.4 0 228 253 251 696.4 0 251 254 169 696.4 0 169 255 160 696.4 0 160 256 200 696.4 0 200 257 211 696.4 0 211 258 159 696.4 0 159 259 156 696.4 0 156 260 129 696.4 0 129 261 138 696.4 0 138 262 155 696.4 0 155 263 154 696.4 0 154 264 184 696.4 0 184 265 207 696.4 0 207 266 324 696.4 0 324 267 3957 696.4 0 3957 268 1914 696.4 0 1914 269 2060 696.4 0 2060 270 152 696.4 0 152 271 133 696.4 0 133 272 95 696.4 0 95 273 88 696.4 0 88 274 113 696.4 0 113 275 139 696.4 0 139 276 128 696.4 0 128 277 291 696.4 0 291 278 204 696.4 0 204 279 280 696.4 0 280 280 555 696.4 0 555 281 153 696.4 0 153 282 778 696.4 0 778 283 131 696.4 0 131 284 114 696.4 0 114 285 93 696.4 0 93 286 133 696.4 0 133 287 105 696.4 0 105 288 116 696.4 0 116 289 152 696.4 0 152 290 137 696.4 0 137 291 141 696.4 0 141 292 119 696.4 0 119 293 126 696.4 0 126 294 156 696.4 0 156 295 148 696.4 0 148 296 124 696.4 0 124 297 142 696.4 0 142 298 240 696.4 0 240 299 149 696.4 0 149 300 122 696.4 0 122 301 124 696.4 0 124 Finished in 6.16 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 148,351 Reads with adapters: 30,944 (20.9%) Reads that were too short: 192 (0.1%) Reads written (passing filters): 30,752 (20.7%) Total basepairs processed: 33,375,653 bp Total written (filtered): 8,398,518 bp (25.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 30944 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 3 0.0 3 0 0 3 16 208 0.0 3 0 204 4 17 28115 0.0 3 27425 665 22 3 18 87 0.0 3 5 77 4 1 19 20 0.0 3 3 2 10 5 20 1923 0.0 3 1866 44 2 11 21 8 0.0 3 2 2 0 4 22 1 0.0 3 0 0 1 23 1 0.0 3 0 0 0 1 24 116 0.0 3 0 0 1 115 26 14 0.0 3 0 0 0 14 27 20 0.0 3 1 0 0 19 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 39 1 0.0 3 1 44 1 0.0 3 0 0 0 1 48 3 0.0 3 0 0 0 3 49 1 0.0 3 0 0 0 1 50 4 0.0 3 0 0 0 4 51 5 0.0 3 0 0 0 5 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 9 0.0 3 0 0 0 9 55 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 20 0.0 3 0 0 0 20 60 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 0 2 62 12 0.0 3 0 0 0 12 63 1 0.0 3 0 0 0 1 66 21 0.0 3 0 0 0 21 67 9 0.0 3 0 0 0 9 68 23 0.0 3 0 0 0 23 69 8 0.0 3 0 0 0 8 70 2 0.0 3 0 0 0 2 71 5 0.0 3 0 0 0 5 74 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 1 1 79 3 0.0 3 0 0 0 3 80 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 3 0.0 3 0 0 0 3 88 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 101 6 0.0 3 0 0 0 6 102 4 0.0 3 0 0 0 4 103 6 0.0 3 0 0 0 6 105 3 0.0 3 0 0 0 3 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 7 0.0 3 0 0 0 7 109 20 0.0 3 0 0 0 20 110 6 0.0 3 0 0 1 5 111 4 0.0 3 0 0 1 3 112 22 0.0 3 0 0 0 22 113 5 0.0 3 0 0 0 5 115 13 0.0 3 0 0 1 12 116 1 0.0 3 0 0 0 1 117 3 0.0 3 0 0 0 3 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 124 2 0.0 3 0 0 0 2 126 3 0.0 3 0 0 0 3 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 136 20 0.0 3 0 0 0 20 137 3 0.0 3 0 0 0 3 138 1 0.0 3 0 0 0 1 139 14 0.0 3 0 0 0 14 140 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 147 6 0.0 3 0 0 0 6 148 2 0.0 3 0 0 0 2 149 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 23 0.0 3 0 0 0 23 164 1 0.0 3 0 0 0 1 166 2 0.0 3 0 0 0 2 167 11 0.0 3 0 0 0 11 168 6 0.0 3 0 0 0 6 169 1 0.0 3 0 0 0 1 170 3 0.0 3 0 0 1 2 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 180 1 0.0 3 0 0 0 1 181 4 0.0 3 0 0 0 4 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 225 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 263 3 0.0 3 0 0 0 3 265 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 281 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.09 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 30,752 Reads with adapters: 1,974 (6.4%) Reads written (passing filters): 30,752 (100.0%) Total basepairs processed: 8,398,518 bp Total written (filtered): 8,392,437 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1974 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 77.0% C: 5.6% G: 16.1% T: 1.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1816 480.5 0 1816 4 157 120.1 0 157 5 1 30.0 0 1