This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 72.37 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 127,956 (4.5%) Reads that were too short: 40,422 (1.4%) Reads written (passing filters): 87,534 (3.1%) Total basepairs processed: 858,556,748 bp Total written (filtered): 23,331,910 bp (2.7%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 127956 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 416 696.4 0 416 7 1072 696.4 0 1072 8 36192 696.4 0 36192 9 27867 696.4 0 27867 10 95 696.4 0 95 11 73 696.4 0 73 12 83 696.4 0 83 13 67 696.4 0 67 14 93 696.4 0 93 15 96 696.4 0 96 16 89 696.4 0 89 17 108 696.4 0 108 18 85 696.4 0 85 19 98 696.4 0 98 20 98 696.4 0 98 21 100 696.4 0 100 22 85 696.4 0 85 23 105 696.4 0 105 24 87 696.4 0 87 25 92 696.4 0 92 26 93 696.4 0 93 27 88 696.4 0 88 28 69 696.4 0 69 29 97 696.4 0 97 30 105 696.4 0 105 31 70 696.4 0 70 32 97 696.4 0 97 33 67 696.4 0 67 34 100 696.4 0 100 35 74 696.4 0 74 36 83 696.4 0 83 37 83 696.4 0 83 38 63 696.4 0 63 39 79 696.4 0 79 40 61 696.4 0 61 41 77 696.4 0 77 42 70 696.4 0 70 43 84 696.4 0 84 44 106 696.4 0 106 45 125 696.4 0 125 46 120 696.4 0 120 47 95 696.4 0 95 48 84 696.4 0 84 49 78 696.4 0 78 50 80 696.4 0 80 51 85 696.4 0 85 52 82 696.4 0 82 53 113 696.4 0 113 54 104 696.4 0 104 55 97 696.4 0 97 56 138 696.4 0 138 57 79 696.4 0 79 58 102 696.4 0 102 59 124 696.4 0 124 60 127 696.4 0 127 61 285 696.4 0 285 62 70 696.4 0 70 63 72 696.4 0 72 64 120 696.4 0 120 65 95 696.4 0 95 66 113 696.4 0 113 67 90 696.4 0 90 68 66 696.4 0 66 69 80 696.4 0 80 70 175 696.4 0 175 71 240 696.4 0 240 72 177 696.4 0 177 73 111 696.4 0 111 74 87 696.4 0 87 75 116 696.4 0 116 76 200 696.4 0 200 77 186 696.4 0 186 78 251 696.4 0 251 79 98 696.4 0 98 80 109 696.4 0 109 81 146 696.4 0 146 82 107 696.4 0 107 83 121 696.4 0 121 84 121 696.4 0 121 85 132 696.4 0 132 86 118 696.4 0 118 87 97 696.4 0 97 88 100 696.4 0 100 89 93 696.4 0 93 90 135 696.4 0 135 91 116 696.4 0 116 92 126 696.4 0 126 93 110 696.4 0 110 94 104 696.4 0 104 95 125 696.4 0 125 96 89 696.4 0 89 97 79 696.4 0 79 98 99 696.4 0 99 99 85 696.4 0 85 100 96 696.4 0 96 101 78 696.4 0 78 102 83 696.4 0 83 103 88 696.4 0 88 104 89 696.4 0 89 105 100 696.4 0 100 106 123 696.4 0 123 107 72 696.4 0 72 108 86 696.4 0 86 109 98 696.4 0 98 110 123 696.4 0 123 111 121 696.4 0 121 112 190 696.4 0 190 113 114 696.4 0 114 114 99 696.4 0 99 115 104 696.4 0 104 116 85 696.4 0 85 117 112 696.4 0 112 118 131 696.4 0 131 119 115 696.4 0 115 120 104 696.4 0 104 121 88 696.4 0 88 122 97 696.4 0 97 123 81 696.4 0 81 124 101 696.4 0 101 125 82 696.4 0 82 126 105 696.4 0 105 127 103 696.4 0 103 128 112 696.4 0 112 129 131 696.4 0 131 130 182 696.4 0 182 131 182 696.4 0 182 132 182 696.4 0 182 133 137 696.4 0 137 134 164 696.4 0 164 135 103 696.4 0 103 136 106 696.4 0 106 137 101 696.4 0 101 138 145 696.4 0 145 139 203 696.4 0 203 140 176 696.4 0 176 141 117 696.4 0 117 142 120 696.4 0 120 143 118 696.4 0 118 144 206 696.4 0 206 145 197 696.4 0 197 146 121 696.4 0 121 147 97 696.4 0 97 148 95 696.4 0 95 149 111 696.4 0 111 150 91 696.4 0 91 151 181 696.4 0 181 152 157 696.4 0 157 153 124 696.4 0 124 154 113 696.4 0 113 155 100 696.4 0 100 156 160 696.4 0 160 157 147 696.4 0 147 158 113 696.4 0 113 159 110 696.4 0 110 160 88 696.4 0 88 161 107 696.4 0 107 162 84 696.4 0 84 163 92 696.4 0 92 164 107 696.4 0 107 165 116 696.4 0 116 166 98 696.4 0 98 167 86 696.4 0 86 168 97 696.4 0 97 169 96 696.4 0 96 170 81 696.4 0 81 171 109 696.4 0 109 172 78 696.4 0 78 173 103 696.4 0 103 174 91 696.4 0 91 175 85 696.4 0 85 176 103 696.4 0 103 177 71 696.4 0 71 178 86 696.4 0 86 179 86 696.4 0 86 180 75 696.4 0 75 181 79 696.4 0 79 182 90 696.4 0 90 183 107 696.4 0 107 184 87 696.4 0 87 185 103 696.4 0 103 186 108 696.4 0 108 187 103 696.4 0 103 188 105 696.4 0 105 189 104 696.4 0 104 190 120 696.4 0 120 191 248 696.4 0 248 192 238 696.4 0 238 193 187 696.4 0 187 194 153 696.4 0 153 195 141 696.4 0 141 196 129 696.4 0 129 197 382 696.4 0 382 198 331 696.4 0 331 199 278 696.4 0 278 200 98 696.4 0 98 201 90 696.4 0 90 202 119 696.4 0 119 203 74 696.4 0 74 204 135 696.4 0 135 205 92 696.4 0 92 206 108 696.4 0 108 207 74 696.4 0 74 208 85 696.4 0 85 209 78 696.4 0 78 210 69 696.4 0 69 211 66 696.4 0 66 212 97 696.4 0 97 213 108 696.4 0 108 214 106 696.4 0 106 215 122 696.4 0 122 216 96 696.4 0 96 217 101 696.4 0 101 218 112 696.4 0 112 219 92 696.4 0 92 220 99 696.4 0 99 221 124 696.4 0 124 222 142 696.4 0 142 223 244 696.4 0 244 224 202 696.4 0 202 225 141 696.4 0 141 226 82 696.4 0 82 227 112 696.4 0 112 228 79 696.4 0 79 229 98 696.4 0 98 230 104 696.4 0 104 231 112 696.4 0 112 232 107 696.4 0 107 233 150 696.4 0 150 234 166 696.4 0 166 235 160 696.4 0 160 236 123 696.4 0 123 237 146 696.4 0 146 238 152 696.4 0 152 239 161 696.4 0 161 240 150 696.4 0 150 241 138 696.4 0 138 242 131 696.4 0 131 243 149 696.4 0 149 244 120 696.4 0 120 245 178 696.4 0 178 246 219 696.4 0 219 247 316 696.4 0 316 248 140 696.4 0 140 249 94 696.4 0 94 250 88 696.4 0 88 251 118 696.4 0 118 252 119 696.4 0 119 253 115 696.4 0 115 254 221 696.4 0 221 255 227 696.4 0 227 256 283 696.4 0 283 257 187 696.4 0 187 258 198 696.4 0 198 259 168 696.4 0 168 260 182 696.4 0 182 261 202 696.4 0 202 262 236 696.4 0 236 263 212 696.4 0 212 264 255 696.4 0 255 265 963 696.4 0 963 266 947 696.4 0 947 267 1296 696.4 0 1296 268 253 696.4 0 253 269 93 696.4 0 93 270 126 696.4 0 126 271 146 696.4 0 146 272 240 696.4 0 240 273 279 696.4 0 279 274 233 696.4 0 233 275 165 696.4 0 165 276 92 696.4 0 92 277 121 696.4 0 121 278 188 696.4 0 188 279 168 696.4 0 168 280 202 696.4 0 202 281 276 696.4 0 276 282 219 696.4 0 219 283 174 696.4 0 174 284 181 696.4 0 181 285 203 696.4 0 203 286 235 696.4 0 235 287 213 696.4 0 213 288 311 696.4 0 311 289 311 696.4 0 311 290 464 696.4 0 464 291 773 696.4 0 773 292 712 696.4 0 712 293 702 696.4 0 702 294 710 696.4 0 710 295 714 696.4 0 714 296 687 696.4 0 687 297 1292 696.4 0 1292 298 5417 696.4 0 5417 299 4188 696.4 0 4188 300 6492 696.4 0 6492 301 1752 696.4 0 1752 Finished in 4.66 s (53 us/read; 1.13 M reads/minute). === Summary === Total reads processed: 87,534 Reads with adapters: 30,369 (34.7%) Reads that were too short: 36 (0.0%) Reads written (passing filters): 30,333 (34.7%) Total basepairs processed: 23,331,910 bp Total written (filtered): 8,207,838 bp (35.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 30369 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 29 0.0 3 0 0 23 5 1 19 479 0.0 3 0 431 28 14 6 20 29054 0.0 4 27205 1474 271 81 23 21 167 0.0 4 78 73 10 5 1 22 15 0.0 4 2 1 5 4 3 23 158 0.0 4 1 1 2 114 40 24 3 0.0 4 1 0 0 1 1 25 2 0.0 4 2 26 1 0.0 4 0 0 0 0 1 28 1 0.0 4 1 35 1 0.0 4 0 1 46 1 0.0 4 1 74 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 126 6 0.0 4 0 0 0 0 6 127 407 0.0 4 0 0 0 0 407 139 1 0.0 4 0 0 0 0 1 142 2 0.0 4 0 0 2 144 1 0.0 4 0 0 0 0 1 146 1 0.0 4 1 152 4 0.0 4 0 0 0 0 4 174 1 0.0 4 0 0 0 0 1 184 2 0.0 4 1 0 0 1 186 2 0.0 4 0 1 1 187 1 0.0 4 1 190 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 3 0.0 4 0 0 0 0 3 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 293 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.12 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 30,333 Reads with adapters: 7,831 (25.8%) Reads written (passing filters): 30,333 (100.0%) Total basepairs processed: 8,207,838 bp Total written (filtered): 8,129,041 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7831 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.0% C: 9.2% G: 86.7% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 15 474.0 0 15 4 13 118.5 0 13 6 3 7.4 0 3 8 4 0.5 0 4 9 2949 0.1 0 392 2557 10 3819 0.0 1 298 3521 11 969 0.0 1 55 914 12 29 0.0 1 0 29 13 12 0.0 1 0 12 14 4 0.0 1 0 4 150 4 0.0 1 4 208 7 0.0 1 5 2 210 1 0.0 1 1 211 1 0.0 1 1 223 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.