This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.49 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 141,957 (5.0%) Reads that were too short: 56,474 (2.0%) Reads written (passing filters): 85,483 (3.0%) Total basepairs processed: 858,556,748 bp Total written (filtered): 23,202,462 bp (2.7%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 141957 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 311 696.4 0 311 7 859 696.4 0 859 8 32621 696.4 0 32621 9 34247 696.4 0 34247 10 98 696.4 0 98 11 55 696.4 0 55 12 92 696.4 0 92 13 62 696.4 0 62 14 80 696.4 0 80 15 91 696.4 0 91 16 86 696.4 0 86 17 75 696.4 0 75 18 72 696.4 0 72 19 66 696.4 0 66 20 72 696.4 0 72 21 68 696.4 0 68 22 82 696.4 0 82 23 94 696.4 0 94 24 82 696.4 0 82 25 70 696.4 0 70 26 65 696.4 0 65 27 64 696.4 0 64 28 76 696.4 0 76 29 70 696.4 0 70 30 59 696.4 0 59 31 50 696.4 0 50 32 53 696.4 0 53 33 57 696.4 0 57 34 75 696.4 0 75 35 62 696.4 0 62 36 80 696.4 0 80 37 68 696.4 0 68 38 44 696.4 0 44 39 75 696.4 0 75 40 40 696.4 0 40 41 72 696.4 0 72 42 55 696.4 0 55 43 60 696.4 0 60 44 69 696.4 0 69 45 120 696.4 0 120 46 90 696.4 0 90 47 74 696.4 0 74 48 82 696.4 0 82 49 54 696.4 0 54 50 56 696.4 0 56 51 65 696.4 0 65 52 63 696.4 0 63 53 75 696.4 0 75 54 72 696.4 0 72 55 87 696.4 0 87 56 122 696.4 0 122 57 91 696.4 0 91 58 77 696.4 0 77 59 127 696.4 0 127 60 108 696.4 0 108 61 335 696.4 0 335 62 68 696.4 0 68 63 81 696.4 0 81 64 94 696.4 0 94 65 82 696.4 0 82 66 79 696.4 0 79 67 75 696.4 0 75 68 71 696.4 0 71 69 89 696.4 0 89 70 198 696.4 0 198 71 234 696.4 0 234 72 171 696.4 0 171 73 80 696.4 0 80 74 72 696.4 0 72 75 70 696.4 0 70 76 99 696.4 0 99 77 124 696.4 0 124 78 167 696.4 0 167 79 57 696.4 0 57 80 81 696.4 0 81 81 123 696.4 0 123 82 91 696.4 0 91 83 67 696.4 0 67 84 68 696.4 0 68 85 89 696.4 0 89 86 61 696.4 0 61 87 75 696.4 0 75 88 70 696.4 0 70 89 61 696.4 0 61 90 92 696.4 0 92 91 93 696.4 0 93 92 87 696.4 0 87 93 100 696.4 0 100 94 92 696.4 0 92 95 113 696.4 0 113 96 79 696.4 0 79 97 84 696.4 0 84 98 80 696.4 0 80 99 84 696.4 0 84 100 108 696.4 0 108 101 92 696.4 0 92 102 72 696.4 0 72 103 82 696.4 0 82 104 62 696.4 0 62 105 60 696.4 0 60 106 81 696.4 0 81 107 53 696.4 0 53 108 64 696.4 0 64 109 77 696.4 0 77 110 91 696.4 0 91 111 83 696.4 0 83 112 119 696.4 0 119 113 76 696.4 0 76 114 61 696.4 0 61 115 72 696.4 0 72 116 55 696.4 0 55 117 82 696.4 0 82 118 76 696.4 0 76 119 91 696.4 0 91 120 85 696.4 0 85 121 66 696.4 0 66 122 60 696.4 0 60 123 51 696.4 0 51 124 76 696.4 0 76 125 60 696.4 0 60 126 70 696.4 0 70 127 92 696.4 0 92 128 91 696.4 0 91 129 87 696.4 0 87 130 128 696.4 0 128 131 114 696.4 0 114 132 136 696.4 0 136 133 83 696.4 0 83 134 100 696.4 0 100 135 113 696.4 0 113 136 73 696.4 0 73 137 86 696.4 0 86 138 129 696.4 0 129 139 162 696.4 0 162 140 133 696.4 0 133 141 93 696.4 0 93 142 94 696.4 0 94 143 91 696.4 0 91 144 152 696.4 0 152 145 170 696.4 0 170 146 78 696.4 0 78 147 76 696.4 0 76 148 64 696.4 0 64 149 65 696.4 0 65 150 57 696.4 0 57 151 166 696.4 0 166 152 118 696.4 0 118 153 85 696.4 0 85 154 68 696.4 0 68 155 89 696.4 0 89 156 116 696.4 0 116 157 101 696.4 0 101 158 105 696.4 0 105 159 78 696.4 0 78 160 67 696.4 0 67 161 77 696.4 0 77 162 71 696.4 0 71 163 71 696.4 0 71 164 55 696.4 0 55 165 75 696.4 0 75 166 65 696.4 0 65 167 59 696.4 0 59 168 81 696.4 0 81 169 69 696.4 0 69 170 55 696.4 0 55 171 75 696.4 0 75 172 54 696.4 0 54 173 66 696.4 0 66 174 82 696.4 0 82 175 62 696.4 0 62 176 53 696.4 0 53 177 64 696.4 0 64 178 66 696.4 0 66 179 67 696.4 0 67 180 65 696.4 0 65 181 60 696.4 0 60 182 72 696.4 0 72 183 99 696.4 0 99 184 65 696.4 0 65 185 74 696.4 0 74 186 63 696.4 0 63 187 78 696.4 0 78 188 77 696.4 0 77 189 71 696.4 0 71 190 93 696.4 0 93 191 209 696.4 0 209 192 255 696.4 0 255 193 197 696.4 0 197 194 106 696.4 0 106 195 102 696.4 0 102 196 98 696.4 0 98 197 330 696.4 0 330 198 349 696.4 0 349 199 319 696.4 0 319 200 82 696.4 0 82 201 91 696.4 0 91 202 83 696.4 0 83 203 56 696.4 0 56 204 94 696.4 0 94 205 97 696.4 0 97 206 82 696.4 0 82 207 71 696.4 0 71 208 70 696.4 0 70 209 75 696.4 0 75 210 73 696.4 0 73 211 66 696.4 0 66 212 65 696.4 0 65 213 86 696.4 0 86 214 87 696.4 0 87 215 95 696.4 0 95 216 87 696.4 0 87 217 85 696.4 0 85 218 80 696.4 0 80 219 70 696.4 0 70 220 87 696.4 0 87 221 110 696.4 0 110 222 143 696.4 0 143 223 262 696.4 0 262 224 188 696.4 0 188 225 105 696.4 0 105 226 84 696.4 0 84 227 71 696.4 0 71 228 56 696.4 0 56 229 73 696.4 0 73 230 68 696.4 0 68 231 108 696.4 0 108 232 79 696.4 0 79 233 97 696.4 0 97 234 109 696.4 0 109 235 98 696.4 0 98 236 81 696.4 0 81 237 81 696.4 0 81 238 116 696.4 0 116 239 111 696.4 0 111 240 111 696.4 0 111 241 76 696.4 0 76 242 79 696.4 0 79 243 73 696.4 0 73 244 73 696.4 0 73 245 116 696.4 0 116 246 140 696.4 0 140 247 145 696.4 0 145 248 87 696.4 0 87 249 71 696.4 0 71 250 81 696.4 0 81 251 85 696.4 0 85 252 81 696.4 0 81 253 77 696.4 0 77 254 157 696.4 0 157 255 153 696.4 0 153 256 224 696.4 0 224 257 117 696.4 0 117 258 133 696.4 0 133 259 115 696.4 0 115 260 171 696.4 0 171 261 217 696.4 0 217 262 140 696.4 0 140 263 146 696.4 0 146 264 154 696.4 0 154 265 366 696.4 0 366 266 466 696.4 0 466 267 500 696.4 0 500 268 188 696.4 0 188 269 76 696.4 0 76 270 83 696.4 0 83 271 110 696.4 0 110 272 118 696.4 0 118 273 114 696.4 0 114 274 151 696.4 0 151 275 90 696.4 0 90 276 77 696.4 0 77 277 101 696.4 0 101 278 153 696.4 0 153 279 199 696.4 0 199 280 225 696.4 0 225 281 285 696.4 0 285 282 243 696.4 0 243 283 209 696.4 0 209 284 229 696.4 0 229 285 291 696.4 0 291 286 246 696.4 0 246 287 299 696.4 0 299 288 426 696.4 0 426 289 556 696.4 0 556 290 745 696.4 0 745 291 1434 696.4 0 1434 292 1334 696.4 0 1334 293 1135 696.4 0 1135 294 1303 696.4 0 1303 295 1208 696.4 0 1208 296 1246 696.4 0 1246 297 2007 696.4 0 2007 298 10227 696.4 0 10227 299 7358 696.4 0 7358 300 11622 696.4 0 11622 301 4483 696.4 0 4483 Finished in 3.94 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 85,483 Reads with adapters: 31,146 (36.4%) Reads that were too short: 41 (0.0%) Reads written (passing filters): 31,105 (36.4%) Total basepairs processed: 23,202,462 bp Total written (filtered): 8,583,429 bp (37.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 31146 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 6 0.0 3 0 0 6 16 182 0.0 3 0 180 2 17 30770 0.0 3 30152 605 11 2 18 85 0.0 3 6 74 5 19 25 0.0 3 0 0 19 6 20 24 0.0 3 0 0 0 24 26 1 0.0 3 0 1 29 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 115 3 0.0 3 0 0 0 3 129 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 1 189 19 0.0 3 0 0 0 19 205 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.10 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 31,105 Reads with adapters: 1,144 (3.7%) Reads written (passing filters): 31,105 (100.0%) Total basepairs processed: 8,583,429 bp Total written (filtered): 8,579,436 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1144 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 49.0% C: 2.7% G: 46.6% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 586 486.0 0 586 4 555 121.5 0 555 5 3 30.4 0 3