This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.54 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 52,759 (1.8%) Reads that were too short: 14,079 (0.5%) Reads written (passing filters): 38,680 (1.4%) Total basepairs processed: 858,556,748 bp Total written (filtered): 9,927,872 bp (1.2%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 52759 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 828 696.4 0 828 7 12059 696.4 0 12059 8 11762 696.4 0 11762 9 87 696.4 0 87 10 34 696.4 0 34 11 38 696.4 0 38 12 45 696.4 0 45 13 29 696.4 0 29 14 37 696.4 0 37 15 35 696.4 0 35 16 44 696.4 0 44 17 52 696.4 0 52 18 37 696.4 0 37 19 50 696.4 0 50 20 32 696.4 0 32 21 31 696.4 0 31 22 36 696.4 0 36 23 39 696.4 0 39 24 33 696.4 0 33 25 30 696.4 0 30 26 41 696.4 0 41 27 34 696.4 0 34 28 74 696.4 0 74 29 78 696.4 0 78 30 48 696.4 0 48 31 73 696.4 0 73 32 374 696.4 0 374 33 45 696.4 0 45 34 32 696.4 0 32 35 35 696.4 0 35 36 45 696.4 0 45 37 44 696.4 0 44 38 35 696.4 0 35 39 44 696.4 0 44 40 61 696.4 0 61 41 58 696.4 0 58 42 91 696.4 0 91 43 90 696.4 0 90 44 107 696.4 0 107 45 38 696.4 0 38 46 49 696.4 0 49 47 54 696.4 0 54 48 36 696.4 0 36 49 41 696.4 0 41 50 38 696.4 0 38 51 28 696.4 0 28 52 39 696.4 0 39 53 48 696.4 0 48 54 75 696.4 0 75 55 39 696.4 0 39 56 29 696.4 0 29 57 43 696.4 0 43 58 31 696.4 0 31 59 50 696.4 0 50 60 47 696.4 0 47 61 40 696.4 0 40 62 70 696.4 0 70 63 73 696.4 0 73 64 603 696.4 0 603 65 507 696.4 0 507 66 613 696.4 0 613 67 137 696.4 0 137 68 73 696.4 0 73 69 158 696.4 0 158 70 52 696.4 0 52 71 42 696.4 0 42 72 21 696.4 0 21 73 30 696.4 0 30 74 35 696.4 0 35 75 30 696.4 0 30 76 37 696.4 0 37 77 30 696.4 0 30 78 30 696.4 0 30 79 32 696.4 0 32 80 43 696.4 0 43 81 78 696.4 0 78 82 30 696.4 0 30 83 46 696.4 0 46 84 53 696.4 0 53 85 31 696.4 0 31 86 38 696.4 0 38 87 33 696.4 0 33 88 40 696.4 0 40 89 76 696.4 0 76 90 81 696.4 0 81 91 92 696.4 0 92 92 337 696.4 0 337 93 244 696.4 0 244 94 122 696.4 0 122 95 40 696.4 0 40 96 26 696.4 0 26 97 35 696.4 0 35 98 39 696.4 0 39 99 53 696.4 0 53 100 44 696.4 0 44 101 61 696.4 0 61 102 30 696.4 0 30 103 29 696.4 0 29 104 32 696.4 0 32 105 48 696.4 0 48 106 29 696.4 0 29 107 45 696.4 0 45 108 48 696.4 0 48 109 54 696.4 0 54 110 33 696.4 0 33 111 48 696.4 0 48 112 42 696.4 0 42 113 32 696.4 0 32 114 39 696.4 0 39 115 34 696.4 0 34 116 40 696.4 0 40 117 56 696.4 0 56 118 56 696.4 0 56 119 40 696.4 0 40 120 50 696.4 0 50 121 52 696.4 0 52 122 48 696.4 0 48 123 42 696.4 0 42 124 51 696.4 0 51 125 45 696.4 0 45 126 42 696.4 0 42 127 39 696.4 0 39 128 51 696.4 0 51 129 47 696.4 0 47 130 56 696.4 0 56 131 69 696.4 0 69 132 39 696.4 0 39 133 53 696.4 0 53 134 47 696.4 0 47 135 70 696.4 0 70 136 68 696.4 0 68 137 76 696.4 0 76 138 64 696.4 0 64 139 56 696.4 0 56 140 50 696.4 0 50 141 60 696.4 0 60 142 60 696.4 0 60 143 45 696.4 0 45 144 49 696.4 0 49 145 40 696.4 0 40 146 47 696.4 0 47 147 102 696.4 0 102 148 64 696.4 0 64 149 76 696.4 0 76 150 90 696.4 0 90 151 71 696.4 0 71 152 99 696.4 0 99 153 112 696.4 0 112 154 61 696.4 0 61 155 112 696.4 0 112 156 99 696.4 0 99 157 119 696.4 0 119 158 99 696.4 0 99 159 65 696.4 0 65 160 71 696.4 0 71 161 68 696.4 0 68 162 52 696.4 0 52 163 73 696.4 0 73 164 54 696.4 0 54 165 48 696.4 0 48 166 74 696.4 0 74 167 126 696.4 0 126 168 155 696.4 0 155 169 142 696.4 0 142 170 60 696.4 0 60 171 111 696.4 0 111 172 101 696.4 0 101 173 95 696.4 0 95 174 49 696.4 0 49 175 162 696.4 0 162 176 175 696.4 0 175 177 140 696.4 0 140 178 88 696.4 0 88 179 60 696.4 0 60 180 44 696.4 0 44 181 42 696.4 0 42 182 65 696.4 0 65 183 68 696.4 0 68 184 69 696.4 0 69 185 61 696.4 0 61 186 76 696.4 0 76 187 68 696.4 0 68 188 191 696.4 0 191 189 159 696.4 0 159 190 109 696.4 0 109 191 85 696.4 0 85 192 62 696.4 0 62 193 74 696.4 0 74 194 49 696.4 0 49 195 68 696.4 0 68 196 80 696.4 0 80 197 93 696.4 0 93 198 74 696.4 0 74 199 78 696.4 0 78 200 74 696.4 0 74 201 80 696.4 0 80 202 76 696.4 0 76 203 91 696.4 0 91 204 88 696.4 0 88 205 97 696.4 0 97 206 71 696.4 0 71 207 60 696.4 0 60 208 74 696.4 0 74 209 58 696.4 0 58 210 70 696.4 0 70 211 79 696.4 0 79 212 84 696.4 0 84 213 93 696.4 0 93 214 89 696.4 0 89 215 116 696.4 0 116 216 117 696.4 0 117 217 76 696.4 0 76 218 135 696.4 0 135 219 78 696.4 0 78 220 94 696.4 0 94 221 221 696.4 0 221 222 292 696.4 0 292 223 367 696.4 0 367 224 279 696.4 0 279 225 132 696.4 0 132 226 205 696.4 0 205 227 205 696.4 0 205 228 111 696.4 0 111 229 73 696.4 0 73 230 77 696.4 0 77 231 73 696.4 0 73 232 75 696.4 0 75 233 88 696.4 0 88 234 90 696.4 0 90 235 78 696.4 0 78 236 73 696.4 0 73 237 140 696.4 0 140 238 120 696.4 0 120 239 86 696.4 0 86 240 100 696.4 0 100 241 254 696.4 0 254 242 194 696.4 0 194 243 418 696.4 0 418 244 193 696.4 0 193 245 194 696.4 0 194 246 112 696.4 0 112 247 90 696.4 0 90 248 109 696.4 0 109 249 103 696.4 0 103 250 129 696.4 0 129 251 119 696.4 0 119 252 141 696.4 0 141 253 204 696.4 0 204 254 132 696.4 0 132 255 106 696.4 0 106 256 86 696.4 0 86 257 109 696.4 0 109 258 98 696.4 0 98 259 67 696.4 0 67 260 66 696.4 0 66 261 70 696.4 0 70 262 123 696.4 0 123 263 109 696.4 0 109 264 122 696.4 0 122 265 113 696.4 0 113 266 109 696.4 0 109 267 98 696.4 0 98 268 208 696.4 0 208 269 177 696.4 0 177 270 495 696.4 0 495 271 263 696.4 0 263 272 237 696.4 0 237 273 260 696.4 0 260 274 156 696.4 0 156 275 157 696.4 0 157 276 90 696.4 0 90 277 150 696.4 0 150 278 143 696.4 0 143 279 114 696.4 0 114 280 86 696.4 0 86 281 101 696.4 0 101 282 123 696.4 0 123 283 167 696.4 0 167 284 155 696.4 0 155 285 111 696.4 0 111 286 104 696.4 0 104 287 121 696.4 0 121 288 89 696.4 0 89 289 112 696.4 0 112 290 169 696.4 0 169 291 242 696.4 0 242 292 233 696.4 0 233 293 196 696.4 0 196 294 94 696.4 0 94 295 100 696.4 0 100 296 127 696.4 0 127 297 137 696.4 0 137 298 213 696.4 0 213 299 269 696.4 0 269 300 247 696.4 0 247 301 234 696.4 0 234 Finished in 1.79 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 38,680 Reads with adapters: 12,164 (31.4%) Reads that were too short: 39 (0.1%) Reads written (passing filters): 12,125 (31.3%) Total basepairs processed: 9,927,872 bp Total written (filtered): 3,353,259 bp (33.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 12164 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 70 0.0 3 0 66 4 17 10857 0.0 3 10625 226 5 1 18 35 0.0 3 1 32 2 19 993 0.0 3 964 20 9 20 146 0.0 3 123 9 0 14 31 2 0.0 3 0 0 0 2 32 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 45 1 0.0 3 1 56 3 0.0 3 0 0 0 3 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 134 2 0.0 3 0 0 0 2 148 3 0.0 3 0 0 0 3 149 2 0.0 3 0 0 0 2 151 3 0.0 3 0 0 0 3 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 160 13 0.0 3 0 0 0 13 161 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 168 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 0 1 173 1 0.0 3 0 1 174 1 0.0 3 1 194 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 237 4 0.0 3 0 0 1 3 257 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.42 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 12,125 Reads with adapters: 135 (1.1%) Reads written (passing filters): 12,125 (100.0%) Total basepairs processed: 3,353,259 bp Total written (filtered): 3,352,832 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 135 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 62.2% C: 16.3% G: 16.3% T: 5.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 123 189.5 0 123 4 5 47.4 0 5 5 4 11.8 0 4 6 3 3.0 0 3