This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATAATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.79 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 404,788 (14.2%) Reads that were too short: 242,267 (8.5%) Reads written (passing filters): 162,521 (5.7%) Total basepairs processed: 858,556,748 bp Total written (filtered): 45,136,516 bp (5.3%) === Adapter 1 === Sequence: ATAATT; Type: regular 5'; Length: 6; Trimmed: 404788 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1303 696.4 0 1303 7 69405 696.4 0 69405 8 2686 696.4 0 2686 9 65829 696.4 0 65829 10 137 696.4 0 137 11 68 696.4 0 68 12 60 696.4 0 60 13 88 696.4 0 88 14 88 696.4 0 88 15 73 696.4 0 73 16 66 696.4 0 66 17 79 696.4 0 79 18 98 696.4 0 98 19 76 696.4 0 76 20 71 696.4 0 71 21 69 696.4 0 69 22 52 696.4 0 52 23 75 696.4 0 75 24 63 696.4 0 63 25 72 696.4 0 72 26 63 696.4 0 63 27 82 696.4 0 82 28 99 696.4 0 99 29 81 696.4 0 81 30 101 696.4 0 101 31 80 696.4 0 80 32 84 696.4 0 84 33 69 696.4 0 69 34 59 696.4 0 59 35 100 696.4 0 100 36 76 696.4 0 76 37 192 696.4 0 192 38 145 696.4 0 145 39 59 696.4 0 59 40 56 696.4 0 56 41 69 696.4 0 69 42 48 696.4 0 48 43 60 696.4 0 60 44 81 696.4 0 81 45 86 696.4 0 86 46 59 696.4 0 59 47 60 696.4 0 60 48 62 696.4 0 62 49 63 696.4 0 63 50 56 696.4 0 56 51 43 696.4 0 43 52 55 696.4 0 55 53 66 696.4 0 66 54 48 696.4 0 48 55 49 696.4 0 49 56 449 696.4 0 449 57 76 696.4 0 76 58 64 696.4 0 64 59 65 696.4 0 65 60 70 696.4 0 70 61 72 696.4 0 72 62 47 696.4 0 47 63 41 696.4 0 41 64 55 696.4 0 55 65 51 696.4 0 51 66 75 696.4 0 75 67 53 696.4 0 53 68 68 696.4 0 68 69 84 696.4 0 84 70 103 696.4 0 103 71 85 696.4 0 85 72 90 696.4 0 90 73 130 696.4 0 130 74 86 696.4 0 86 75 178 696.4 0 178 76 127 696.4 0 127 77 237 696.4 0 237 78 172 696.4 0 172 79 143 696.4 0 143 80 122 696.4 0 122 81 164 696.4 0 164 82 136 696.4 0 136 83 109 696.4 0 109 84 106 696.4 0 106 85 117 696.4 0 117 86 100 696.4 0 100 87 423 696.4 0 423 88 152 696.4 0 152 89 91 696.4 0 91 90 59 696.4 0 59 91 59 696.4 0 59 92 70 696.4 0 70 93 76 696.4 0 76 94 96 696.4 0 96 95 73 696.4 0 73 96 558 696.4 0 558 97 160 696.4 0 160 98 64 696.4 0 64 99 148 696.4 0 148 100 119 696.4 0 119 101 98 696.4 0 98 102 99 696.4 0 99 103 133 696.4 0 133 104 104 696.4 0 104 105 106 696.4 0 106 106 142 696.4 0 142 107 150 696.4 0 150 108 171 696.4 0 171 109 96 696.4 0 96 110 160 696.4 0 160 111 180 696.4 0 180 112 184 696.4 0 184 113 123 696.4 0 123 114 139 696.4 0 139 115 117 696.4 0 117 116 88 696.4 0 88 117 87 696.4 0 87 118 128 696.4 0 128 119 157 696.4 0 157 120 128 696.4 0 128 121 124 696.4 0 124 122 115 696.4 0 115 123 177 696.4 0 177 124 194 696.4 0 194 125 183 696.4 0 183 126 133 696.4 0 133 127 106 696.4 0 106 128 96 696.4 0 96 129 108 696.4 0 108 130 118 696.4 0 118 131 110 696.4 0 110 132 119 696.4 0 119 133 110 696.4 0 110 134 155 696.4 0 155 135 112 696.4 0 112 136 106 696.4 0 106 137 110 696.4 0 110 138 195 696.4 0 195 139 250 696.4 0 250 140 352 696.4 0 352 141 171 696.4 0 171 142 76 696.4 0 76 143 79 696.4 0 79 144 69 696.4 0 69 145 92 696.4 0 92 146 113 696.4 0 113 147 133 696.4 0 133 148 129 696.4 0 129 149 83 696.4 0 83 150 71 696.4 0 71 151 43 696.4 0 43 152 72 696.4 0 72 153 101 696.4 0 101 154 110 696.4 0 110 155 148 696.4 0 148 156 131 696.4 0 131 157 122 696.4 0 122 158 145 696.4 0 145 159 986 696.4 0 986 160 1016 696.4 0 1016 161 1129 696.4 0 1129 162 79 696.4 0 79 163 82 696.4 0 82 164 101 696.4 0 101 165 71 696.4 0 71 166 92 696.4 0 92 167 101 696.4 0 101 168 84 696.4 0 84 169 81 696.4 0 81 170 81 696.4 0 81 171 57 696.4 0 57 172 115 696.4 0 115 173 105 696.4 0 105 174 123 696.4 0 123 175 112 696.4 0 112 176 127 696.4 0 127 177 65 696.4 0 65 178 53 696.4 0 53 179 78 696.4 0 78 180 99 696.4 0 99 181 91 696.4 0 91 182 93 696.4 0 93 183 89 696.4 0 89 184 81 696.4 0 81 185 118 696.4 0 118 186 106 696.4 0 106 187 100 696.4 0 100 188 77 696.4 0 77 189 72 696.4 0 72 190 51 696.4 0 51 191 65 696.4 0 65 192 81 696.4 0 81 193 103 696.4 0 103 194 110 696.4 0 110 195 107 696.4 0 107 196 73 696.4 0 73 197 96 696.4 0 96 198 92 696.4 0 92 199 121 696.4 0 121 200 92 696.4 0 92 201 83 696.4 0 83 202 110 696.4 0 110 203 107 696.4 0 107 204 82 696.4 0 82 205 140 696.4 0 140 206 216 696.4 0 216 207 385 696.4 0 385 208 236 696.4 0 236 209 253 696.4 0 253 210 197 696.4 0 197 211 153 696.4 0 153 212 421 696.4 0 421 213 300 696.4 0 300 214 302 696.4 0 302 215 99 696.4 0 99 216 95 696.4 0 95 217 129 696.4 0 129 218 414 696.4 0 414 219 184 696.4 0 184 220 135 696.4 0 135 221 178 696.4 0 178 222 146 696.4 0 146 223 149 696.4 0 149 224 133 696.4 0 133 225 185 696.4 0 185 226 105 696.4 0 105 227 207 696.4 0 207 228 443 696.4 0 443 229 181 696.4 0 181 230 146 696.4 0 146 231 192 696.4 0 192 232 913 696.4 0 913 233 602 696.4 0 602 234 903 696.4 0 903 235 176 696.4 0 176 236 146 696.4 0 146 237 241 696.4 0 241 238 463 696.4 0 463 239 186 696.4 0 186 240 358 696.4 0 358 241 232 696.4 0 232 242 270 696.4 0 270 243 309 696.4 0 309 244 194 696.4 0 194 245 145 696.4 0 145 246 126 696.4 0 126 247 204 696.4 0 204 248 333 696.4 0 333 249 436 696.4 0 436 250 850 696.4 0 850 251 1051 696.4 0 1051 252 1843 696.4 0 1843 253 3982 696.4 0 3982 254 16786 696.4 0 16786 255 86900 696.4 0 86900 256 70100 696.4 0 70100 257 42618 696.4 0 42618 258 1859 696.4 0 1859 259 147 696.4 0 147 260 153 696.4 0 153 261 155 696.4 0 155 262 99 696.4 0 99 263 105 696.4 0 105 264 110 696.4 0 110 265 86 696.4 0 86 266 188 696.4 0 188 267 156 696.4 0 156 268 206 696.4 0 206 269 58 696.4 0 58 270 50 696.4 0 50 271 63 696.4 0 63 272 73 696.4 0 73 273 83 696.4 0 83 274 66 696.4 0 66 275 67 696.4 0 67 276 65 696.4 0 65 277 58 696.4 0 58 278 83 696.4 0 83 279 75 696.4 0 75 280 78 696.4 0 78 281 65 696.4 0 65 282 81 696.4 0 81 283 91 696.4 0 91 284 98 696.4 0 98 285 50 696.4 0 50 286 56 696.4 0 56 287 64 696.4 0 64 288 52 696.4 0 52 289 62 696.4 0 62 290 46 696.4 0 46 291 72 696.4 0 72 292 60 696.4 0 60 293 58 696.4 0 58 294 87 696.4 0 87 295 110 696.4 0 110 296 78 696.4 0 78 297 70 696.4 0 70 298 81 696.4 0 81 299 103 696.4 0 103 300 159 696.4 0 159 301 251 696.4 0 251 Finished in 8.38 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 162,521 Reads with adapters: 70,354 (43.3%) Reads that were too short: 42 (0.0%) Reads written (passing filters): 70,312 (43.3%) Total basepairs processed: 45,136,516 bp Total written (filtered): 19,185,636 bp (42.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 70354 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 1 2 18 19 0.0 3 0 0 13 3 3 19 518 0.0 3 0 448 40 20 10 20 68749 0.0 4 65603 2529 468 109 40 21 183 0.0 4 26 149 6 1 1 22 21 0.0 4 6 1 11 1 2 23 79 0.0 4 1 0 0 58 20 24 1 0.0 4 0 0 0 0 1 25 2 0.0 4 1 0 0 0 1 28 2 0.0 4 1 0 0 0 1 29 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 36 1 0.0 4 1 37 1 0.0 4 1 39 1 0.0 4 0 0 0 0 1 61 2 0.0 4 0 0 0 0 2 63 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 113 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 7 0.0 4 0 0 0 0 7 127 702 0.0 4 0 0 0 0 702 128 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 131 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 153 6 0.0 4 0 0 0 0 6 168 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 1 186 1 0.0 4 0 0 0 0 1 189 2 0.0 4 0 0 0 0 2 190 2 0.0 4 0 0 0 0 2 191 1 0.0 4 0 0 0 1 196 1 0.0 4 0 0 0 0 1 197 2 0.0 4 0 0 0 0 2 201 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 2 0.0 4 0 0 0 0 2 215 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 1 2 229 3 0.0 4 0 0 0 0 3 230 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 252 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 260 2 0.0 4 0 0 0 0 2 261 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.52 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 70,312 Reads with adapters: 7,383 (10.5%) Reads written (passing filters): 70,312 (100.0%) Total basepairs processed: 19,185,636 bp Total written (filtered): 19,094,647 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7383 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.3% C: 11.5% G: 81.6% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 228 1098.6 0 228 4 13 274.7 0 13 5 1 68.7 0 1 9 22 0.3 0 2 20 10 98 0.1 1 1 97 11 6112 0.0 1 331 5781 12 819 0.0 1 21 798 13 25 0.0 1 2 23 14 2 0.0 1 0 2 15 6 0.0 1 0 6 123 1 0.0 1 0 1 149 1 0.0 1 0 1 152 7 0.0 1 7 153 1 0.0 1 1 191 7 0.0 1 0 7 200 1 0.0 1 1 210 29 0.0 1 26 3 222 3 0.0 1 0 3 225 2 0.0 1 2 267 5 0.0 1 5 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.