This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATAATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.38 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 589,094 (20.7%) Reads that were too short: 423,609 (14.9%) Reads written (passing filters): 165,485 (5.8%) Total basepairs processed: 858,556,748 bp Total written (filtered): 45,947,022 bp (5.4%) === Adapter 1 === Sequence: ATAATT; Type: regular 5'; Length: 6; Trimmed: 589094 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1026 696.4 0 1026 7 78574 696.4 0 78574 8 1804 696.4 0 1804 9 62363 696.4 0 62363 10 115 696.4 0 115 11 42 696.4 0 42 12 34 696.4 0 34 13 35 696.4 0 35 14 48 696.4 0 48 15 43 696.4 0 43 16 57 696.4 0 57 17 32 696.4 0 32 18 43 696.4 0 43 19 47 696.4 0 47 20 42 696.4 0 42 21 33 696.4 0 33 22 38 696.4 0 38 23 28 696.4 0 28 24 35 696.4 0 35 25 56 696.4 0 56 26 40 696.4 0 40 27 42 696.4 0 42 28 52 696.4 0 52 29 46 696.4 0 46 30 43 696.4 0 43 31 59 696.4 0 59 32 37 696.4 0 37 33 36 696.4 0 36 34 25 696.4 0 25 35 36 696.4 0 36 36 31 696.4 0 31 37 101 696.4 0 101 38 92 696.4 0 92 39 38 696.4 0 38 40 35 696.4 0 35 41 31 696.4 0 31 42 31 696.4 0 31 43 39 696.4 0 39 44 56 696.4 0 56 45 48 696.4 0 48 46 26 696.4 0 26 47 25 696.4 0 25 48 32 696.4 0 32 49 30 696.4 0 30 50 39 696.4 0 39 51 34 696.4 0 34 52 34 696.4 0 34 53 33 696.4 0 33 54 28 696.4 0 28 55 31 696.4 0 31 56 36 696.4 0 36 57 24 696.4 0 24 58 28 696.4 0 28 59 30 696.4 0 30 60 51 696.4 0 51 61 36 696.4 0 36 62 23 696.4 0 23 63 25 696.4 0 25 64 29 696.4 0 29 65 40 696.4 0 40 66 54 696.4 0 54 67 51 696.4 0 51 68 58 696.4 0 58 69 45 696.4 0 45 70 86 696.4 0 86 71 69 696.4 0 69 72 53 696.4 0 53 73 89 696.4 0 89 74 62 696.4 0 62 75 54 696.4 0 54 76 79 696.4 0 79 77 172 696.4 0 172 78 264 696.4 0 264 79 115 696.4 0 115 80 145 696.4 0 145 81 141 696.4 0 141 82 142 696.4 0 142 83 108 696.4 0 108 84 129 696.4 0 129 85 138 696.4 0 138 86 191 696.4 0 191 87 481 696.4 0 481 88 212 696.4 0 212 89 99 696.4 0 99 90 82 696.4 0 82 91 85 696.4 0 85 92 101 696.4 0 101 93 105 696.4 0 105 94 141 696.4 0 141 95 117 696.4 0 117 96 153 696.4 0 153 97 152 696.4 0 152 98 148 696.4 0 148 99 125 696.4 0 125 100 111 696.4 0 111 101 94 696.4 0 94 102 93 696.4 0 93 103 103 696.4 0 103 104 117 696.4 0 117 105 127 696.4 0 127 106 128 696.4 0 128 107 129 696.4 0 129 108 126 696.4 0 126 109 100 696.4 0 100 110 127 696.4 0 127 111 149 696.4 0 149 112 150 696.4 0 150 113 113 696.4 0 113 114 120 696.4 0 120 115 110 696.4 0 110 116 75 696.4 0 75 117 90 696.4 0 90 118 107 696.4 0 107 119 177 696.4 0 177 120 108 696.4 0 108 121 160 696.4 0 160 122 111 696.4 0 111 123 169 696.4 0 169 124 155 696.4 0 155 125 145 696.4 0 145 126 114 696.4 0 114 127 91 696.4 0 91 128 122 696.4 0 122 129 104 696.4 0 104 130 106 696.4 0 106 131 116 696.4 0 116 132 108 696.4 0 108 133 150 696.4 0 150 134 137 696.4 0 137 135 119 696.4 0 119 136 100 696.4 0 100 137 109 696.4 0 109 138 145 696.4 0 145 139 296 696.4 0 296 140 384 696.4 0 384 141 240 696.4 0 240 142 106 696.4 0 106 143 88 696.4 0 88 144 82 696.4 0 82 145 133 696.4 0 133 146 110 696.4 0 110 147 145 696.4 0 145 148 132 696.4 0 132 149 89 696.4 0 89 150 91 696.4 0 91 151 45 696.4 0 45 152 63 696.4 0 63 153 97 696.4 0 97 154 119 696.4 0 119 155 143 696.4 0 143 156 130 696.4 0 130 157 131 696.4 0 131 158 154 696.4 0 154 159 1126 696.4 0 1126 160 1260 696.4 0 1260 161 1273 696.4 0 1273 162 97 696.4 0 97 163 88 696.4 0 88 164 85 696.4 0 85 165 100 696.4 0 100 166 88 696.4 0 88 167 132 696.4 0 132 168 92 696.4 0 92 169 89 696.4 0 89 170 86 696.4 0 86 171 59 696.4 0 59 172 108 696.4 0 108 173 135 696.4 0 135 174 113 696.4 0 113 175 104 696.4 0 104 176 101 696.4 0 101 177 79 696.4 0 79 178 82 696.4 0 82 179 72 696.4 0 72 180 101 696.4 0 101 181 99 696.4 0 99 182 81 696.4 0 81 183 77 696.4 0 77 184 111 696.4 0 111 185 142 696.4 0 142 186 113 696.4 0 113 187 132 696.4 0 132 188 70 696.4 0 70 189 70 696.4 0 70 190 88 696.4 0 88 191 75 696.4 0 75 192 111 696.4 0 111 193 123 696.4 0 123 194 150 696.4 0 150 195 103 696.4 0 103 196 123 696.4 0 123 197 113 696.4 0 113 198 123 696.4 0 123 199 115 696.4 0 115 200 112 696.4 0 112 201 98 696.4 0 98 202 114 696.4 0 114 203 117 696.4 0 117 204 121 696.4 0 121 205 132 696.4 0 132 206 267 696.4 0 267 207 300 696.4 0 300 208 243 696.4 0 243 209 220 696.4 0 220 210 265 696.4 0 265 211 158 696.4 0 158 212 229 696.4 0 229 213 190 696.4 0 190 214 227 696.4 0 227 215 124 696.4 0 124 216 124 696.4 0 124 217 142 696.4 0 142 218 634 696.4 0 634 219 260 696.4 0 260 220 167 696.4 0 167 221 220 696.4 0 220 222 145 696.4 0 145 223 170 696.4 0 170 224 165 696.4 0 165 225 215 696.4 0 215 226 139 696.4 0 139 227 256 696.4 0 256 228 710 696.4 0 710 229 204 696.4 0 204 230 135 696.4 0 135 231 174 696.4 0 174 232 455 696.4 0 455 233 268 696.4 0 268 234 419 696.4 0 419 235 242 696.4 0 242 236 154 696.4 0 154 237 374 696.4 0 374 238 933 696.4 0 933 239 289 696.4 0 289 240 507 696.4 0 507 241 361 696.4 0 361 242 370 696.4 0 370 243 458 696.4 0 458 244 304 696.4 0 304 245 260 696.4 0 260 246 192 696.4 0 192 247 344 696.4 0 344 248 495 696.4 0 495 249 698 696.4 0 698 250 1281 696.4 0 1281 251 1804 696.4 0 1804 252 2969 696.4 0 2969 253 5752 696.4 0 5752 254 20731 696.4 0 20731 255 154954 696.4 0 154954 256 133411 696.4 0 133411 257 81985 696.4 0 81985 258 2671 696.4 0 2671 259 153 696.4 0 153 260 138 696.4 0 138 261 157 696.4 0 157 262 105 696.4 0 105 263 117 696.4 0 117 264 100 696.4 0 100 265 93 696.4 0 93 266 217 696.4 0 217 267 190 696.4 0 190 268 197 696.4 0 197 269 71 696.4 0 71 270 63 696.4 0 63 271 82 696.4 0 82 272 81 696.4 0 81 273 91 696.4 0 91 274 89 696.4 0 89 275 65 696.4 0 65 276 62 696.4 0 62 277 54 696.4 0 54 278 79 696.4 0 79 279 102 696.4 0 102 280 79 696.4 0 79 281 70 696.4 0 70 282 104 696.4 0 104 283 94 696.4 0 94 284 110 696.4 0 110 285 56 696.4 0 56 286 43 696.4 0 43 287 64 696.4 0 64 288 77 696.4 0 77 289 52 696.4 0 52 290 63 696.4 0 63 291 66 696.4 0 66 292 70 696.4 0 70 293 61 696.4 0 61 294 100 696.4 0 100 295 132 696.4 0 132 296 67 696.4 0 67 297 76 696.4 0 76 298 85 696.4 0 85 299 94 696.4 0 94 300 198 696.4 0 198 301 294 696.4 0 294 Finished in 7.67 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 165,485 Reads with adapters: 64,643 (39.1%) Reads that were too short: 39 (0.0%) Reads written (passing filters): 64,604 (39.0%) Total basepairs processed: 45,947,022 bp Total written (filtered): 17,767,308 bp (38.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 64643 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 8 0.0 3 0 0 7 1 16 358 0.0 3 0 347 9 2 17 64032 0.0 3 62551 1397 78 6 18 108 0.0 3 8 95 5 19 52 0.0 3 6 1 31 14 20 35 0.0 3 0 0 0 35 27 2 0.0 3 0 0 0 2 28 1 0.0 3 1 30 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 115 3 0.0 3 0 0 0 3 127 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 163 3 0.0 3 0 0 0 3 168 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 207 2 0.0 3 0 0 0 2 214 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 228 2 0.0 3 0 0 1 1 246 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 281 6 0.0 3 0 0 0 6 282 4 0.0 3 0 0 0 4 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.28 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 64,604 Reads with adapters: 2,823 (4.4%) Reads written (passing filters): 64,604 (100.0%) Total basepairs processed: 17,767,308 bp Total written (filtered): 17,758,497 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2823 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.1% C: 6.7% G: 14.6% T: 2.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2485 1009.4 0 2485 4 335 252.4 0 335 5 2 63.1 0 2 6 1 15.8 0 1