This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.80 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 139,132 (4.9%) Reads that were too short: 34,219 (1.2%) Reads written (passing filters): 104,913 (3.7%) Total basepairs processed: 858,556,748 bp Total written (filtered): 25,483,042 bp (3.0%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 139132 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3917 696.4 0 3917 7 1505 696.4 0 1505 8 35074 696.4 0 35074 9 29414 696.4 0 29414 10 61 696.4 0 61 11 33 696.4 0 33 12 19 696.4 0 19 13 69 696.4 0 69 14 31 696.4 0 31 15 19 696.4 0 19 16 38 696.4 0 38 17 24 696.4 0 24 18 35 696.4 0 35 19 30 696.4 0 30 20 30 696.4 0 30 21 202 696.4 0 202 22 47 696.4 0 47 23 45 696.4 0 45 24 40 696.4 0 40 25 19 696.4 0 19 26 122 696.4 0 122 27 112 696.4 0 112 28 25 696.4 0 25 29 58 696.4 0 58 30 24 696.4 0 24 31 47 696.4 0 47 32 28 696.4 0 28 33 35 696.4 0 35 34 39 696.4 0 39 35 39 696.4 0 39 36 26 696.4 0 26 37 40 696.4 0 40 38 44 696.4 0 44 39 56 696.4 0 56 40 30 696.4 0 30 41 38 696.4 0 38 42 35 696.4 0 35 43 19 696.4 0 19 44 41 696.4 0 41 45 29 696.4 0 29 46 22 696.4 0 22 47 39 696.4 0 39 48 46 696.4 0 46 49 23 696.4 0 23 50 32 696.4 0 32 51 41 696.4 0 41 52 45 696.4 0 45 53 32 696.4 0 32 54 40 696.4 0 40 55 30 696.4 0 30 56 32 696.4 0 32 57 15 696.4 0 15 58 24 696.4 0 24 59 34 696.4 0 34 60 27 696.4 0 27 61 31 696.4 0 31 62 21 696.4 0 21 63 36 696.4 0 36 64 49 696.4 0 49 65 96 696.4 0 96 66 63 696.4 0 63 67 42 696.4 0 42 68 44 696.4 0 44 69 92 696.4 0 92 70 38 696.4 0 38 71 41 696.4 0 41 72 28 696.4 0 28 73 37 696.4 0 37 74 60 696.4 0 60 75 56 696.4 0 56 76 57 696.4 0 57 77 60 696.4 0 60 78 66 696.4 0 66 79 419 696.4 0 419 80 274 696.4 0 274 81 110 696.4 0 110 82 60 696.4 0 60 83 53 696.4 0 53 84 102 696.4 0 102 85 53 696.4 0 53 86 54 696.4 0 54 87 66 696.4 0 66 88 37 696.4 0 37 89 61 696.4 0 61 90 55 696.4 0 55 91 36 696.4 0 36 92 32 696.4 0 32 93 91 696.4 0 91 94 51 696.4 0 51 95 57 696.4 0 57 96 45 696.4 0 45 97 35 696.4 0 35 98 38 696.4 0 38 99 78 696.4 0 78 100 70 696.4 0 70 101 67 696.4 0 67 102 40 696.4 0 40 103 25 696.4 0 25 104 27 696.4 0 27 105 29 696.4 0 29 106 27 696.4 0 27 107 54 696.4 0 54 108 33 696.4 0 33 109 45 696.4 0 45 110 40 696.4 0 40 111 47 696.4 0 47 112 148 696.4 0 148 113 96 696.4 0 96 114 105 696.4 0 105 115 55 696.4 0 55 116 60 696.4 0 60 117 367 696.4 0 367 118 178 696.4 0 178 119 212 696.4 0 212 120 57 696.4 0 57 121 68 696.4 0 68 122 40 696.4 0 40 123 58 696.4 0 58 124 45 696.4 0 45 125 64 696.4 0 64 126 73 696.4 0 73 127 83 696.4 0 83 128 116 696.4 0 116 129 85 696.4 0 85 130 134 696.4 0 134 131 665 696.4 0 665 132 325 696.4 0 325 133 572 696.4 0 572 134 101 696.4 0 101 135 67 696.4 0 67 136 110 696.4 0 110 137 86 696.4 0 86 138 241 696.4 0 241 139 88 696.4 0 88 140 95 696.4 0 95 141 35 696.4 0 35 142 52 696.4 0 52 143 52 696.4 0 52 144 148 696.4 0 148 145 163 696.4 0 163 146 149 696.4 0 149 147 207 696.4 0 207 148 72 696.4 0 72 149 58 696.4 0 58 150 52 696.4 0 52 151 39 696.4 0 39 152 42 696.4 0 42 153 59 696.4 0 59 154 72 696.4 0 72 155 60 696.4 0 60 156 83 696.4 0 83 157 116 696.4 0 116 158 89 696.4 0 89 159 73 696.4 0 73 160 63 696.4 0 63 161 81 696.4 0 81 162 62 696.4 0 62 163 50 696.4 0 50 164 70 696.4 0 70 165 88 696.4 0 88 166 66 696.4 0 66 167 103 696.4 0 103 168 166 696.4 0 166 169 104 696.4 0 104 170 95 696.4 0 95 171 96 696.4 0 96 172 109 696.4 0 109 173 190 696.4 0 190 174 97 696.4 0 97 175 144 696.4 0 144 176 545 696.4 0 545 177 487 696.4 0 487 178 2728 696.4 0 2728 179 1526 696.4 0 1526 180 2681 696.4 0 2681 181 1883 696.4 0 1883 182 1871 696.4 0 1871 183 1489 696.4 0 1489 184 708 696.4 0 708 185 162 696.4 0 162 186 94 696.4 0 94 187 64 696.4 0 64 188 86 696.4 0 86 189 143 696.4 0 143 190 85 696.4 0 85 191 119 696.4 0 119 192 99 696.4 0 99 193 97 696.4 0 97 194 222 696.4 0 222 195 424 696.4 0 424 196 1818 696.4 0 1818 197 2435 696.4 0 2435 198 1028 696.4 0 1028 199 306 696.4 0 306 200 167 696.4 0 167 201 798 696.4 0 798 202 1125 696.4 0 1125 203 990 696.4 0 990 204 555 696.4 0 555 205 45 696.4 0 45 206 50 696.4 0 50 207 44 696.4 0 44 208 35 696.4 0 35 209 64 696.4 0 64 210 53 696.4 0 53 211 53 696.4 0 53 212 54 696.4 0 54 213 67 696.4 0 67 214 50 696.4 0 50 215 219 696.4 0 219 216 160 696.4 0 160 217 210 696.4 0 210 218 88 696.4 0 88 219 60 696.4 0 60 220 77 696.4 0 77 221 172 696.4 0 172 222 693 696.4 0 693 223 1004 696.4 0 1004 224 668 696.4 0 668 225 435 696.4 0 435 226 65 696.4 0 65 227 45 696.4 0 45 228 56 696.4 0 56 229 51 696.4 0 51 230 57 696.4 0 57 231 69 696.4 0 69 232 79 696.4 0 79 233 75 696.4 0 75 234 425 696.4 0 425 235 171 696.4 0 171 236 310 696.4 0 310 237 96 696.4 0 96 238 93 696.4 0 93 239 348 696.4 0 348 240 383 696.4 0 383 241 519 696.4 0 519 242 365 696.4 0 365 243 375 696.4 0 375 244 242 696.4 0 242 245 157 696.4 0 157 246 83 696.4 0 83 247 61 696.4 0 61 248 54 696.4 0 54 249 58 696.4 0 58 250 75 696.4 0 75 251 128 696.4 0 128 252 165 696.4 0 165 253 154 696.4 0 154 254 226 696.4 0 226 255 130 696.4 0 130 256 121 696.4 0 121 257 568 696.4 0 568 258 676 696.4 0 676 259 1728 696.4 0 1728 260 1079 696.4 0 1079 261 1116 696.4 0 1116 262 309 696.4 0 309 263 314 696.4 0 314 264 1085 696.4 0 1085 265 3349 696.4 0 3349 266 1851 696.4 0 1851 267 1588 696.4 0 1588 268 46 696.4 0 46 269 62 696.4 0 62 270 69 696.4 0 69 271 71 696.4 0 71 272 56 696.4 0 56 273 160 696.4 0 160 274 209 696.4 0 209 275 204 696.4 0 204 276 260 696.4 0 260 277 318 696.4 0 318 278 289 696.4 0 289 279 267 696.4 0 267 280 68 696.4 0 68 281 70 696.4 0 70 282 53 696.4 0 53 283 85 696.4 0 85 284 83 696.4 0 83 285 99 696.4 0 99 286 103 696.4 0 103 287 100 696.4 0 100 288 89 696.4 0 89 289 96 696.4 0 96 290 173 696.4 0 173 291 1313 696.4 0 1313 292 968 696.4 0 968 293 1389 696.4 0 1389 294 400 696.4 0 400 295 135 696.4 0 135 296 114 696.4 0 114 297 193 696.4 0 193 298 161 696.4 0 161 299 219 696.4 0 219 300 228 696.4 0 228 301 199 696.4 0 199 Finished in 5.20 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 104,913 Reads with adapters: 32,824 (31.3%) Reads that were too short: 40 (0.0%) Reads written (passing filters): 32,784 (31.2%) Total basepairs processed: 25,483,042 bp Total written (filtered): 8,786,705 bp (34.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 32824 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 6 0.0 3 0 0 0 4 2 18 25 0.0 3 0 0 10 11 4 19 278 0.0 3 0 230 19 19 10 20 30962 0.0 4 28925 1638 274 93 32 21 81 0.0 4 15 58 5 2 1 22 7 0.0 4 1 0 4 1 1 23 164 0.0 4 3 1 0 118 42 24 3 0.0 4 0 0 0 1 2 29 2 0.0 4 0 0 0 0 2 30 1 0.0 4 1 31 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 39 3 0.0 4 0 0 0 1 2 40 2 0.0 4 0 0 0 0 2 41 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 90 2 0.0 4 0 0 0 0 2 92 1 0.0 4 0 0 1 105 1 0.0 4 1 109 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 11 0.0 4 0 0 0 0 11 127 1199 0.0 4 0 0 0 0 1199 128 2 0.0 4 0 0 0 0 2 130 36 0.0 4 0 0 0 0 36 133 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 157 2 0.0 4 0 0 0 0 2 180 1 0.0 4 0 0 0 1 190 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 215 2 0.0 4 0 0 0 1 1 220 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 1 0.0 4 0 0 0 0 1 264 2 0.0 4 0 0 0 0 2 283 2 0.0 4 0 0 0 0 2 285 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 293 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.19 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 32,784 Reads with adapters: 7,687 (23.4%) Reads written (passing filters): 32,784 (100.0%) Total basepairs processed: 8,786,705 bp Total written (filtered): 8,707,442 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7687 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.4% C: 8.2% G: 87.2% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 10 512.2 0 10 4 2 128.1 0 2 5 1 32.0 0 1 7 2 2.0 0 2 8 4 0.5 0 4 9 2029 0.1 0 260 1769 10 4795 0.0 1 389 4406 11 781 0.0 1 41 740 12 28 0.0 1 1 27 13 12 0.0 1 0 12 138 1 0.0 1 1 148 1 0.0 1 1 150 9 0.0 1 9 151 1 0.0 1 1 152 1 0.0 1 0 1 153 1 0.0 1 1 158 2 0.0 1 0 2 162 1 0.0 1 0 1 191 2 0.0 1 0 2 210 1 0.0 1 1 223 2 0.0 1 2 272 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.