This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.20 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 114,781 (4.0%) Reads that were too short: 20,292 (0.7%) Reads written (passing filters): 94,489 (3.3%) Total basepairs processed: 858,556,748 bp Total written (filtered): 24,275,131 bp (2.8%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 114781 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3845 696.4 0 3845 7 1186 696.4 0 1186 8 32858 696.4 0 32858 9 34033 696.4 0 34033 10 49 696.4 0 49 11 18 696.4 0 18 12 16 696.4 0 16 13 13 696.4 0 13 14 21 696.4 0 21 15 12 696.4 0 12 16 22 696.4 0 22 17 24 696.4 0 24 18 27 696.4 0 27 19 29 696.4 0 29 20 10 696.4 0 10 21 32 696.4 0 32 22 31 696.4 0 31 23 24 696.4 0 24 24 19 696.4 0 19 25 12 696.4 0 12 26 22 696.4 0 22 27 20 696.4 0 20 28 17 696.4 0 17 29 45 696.4 0 45 30 22 696.4 0 22 31 35 696.4 0 35 32 22 696.4 0 22 33 27 696.4 0 27 34 30 696.4 0 30 35 27 696.4 0 27 36 18 696.4 0 18 37 43 696.4 0 43 38 30 696.4 0 30 39 43 696.4 0 43 40 36 696.4 0 36 41 30 696.4 0 30 42 29 696.4 0 29 43 20 696.4 0 20 44 26 696.4 0 26 45 22 696.4 0 22 46 10 696.4 0 10 47 27 696.4 0 27 48 26 696.4 0 26 49 27 696.4 0 27 50 21 696.4 0 21 51 18 696.4 0 18 52 24 696.4 0 24 53 33 696.4 0 33 54 27 696.4 0 27 55 29 696.4 0 29 56 12 696.4 0 12 57 27 696.4 0 27 58 24 696.4 0 24 59 17 696.4 0 17 60 22 696.4 0 22 61 25 696.4 0 25 62 29 696.4 0 29 63 23 696.4 0 23 64 38 696.4 0 38 65 25 696.4 0 25 66 36 696.4 0 36 67 26 696.4 0 26 68 26 696.4 0 26 69 117 696.4 0 117 70 30 696.4 0 30 71 35 696.4 0 35 72 24 696.4 0 24 73 16 696.4 0 16 74 23 696.4 0 23 75 30 696.4 0 30 76 26 696.4 0 26 77 35 696.4 0 35 78 38 696.4 0 38 79 386 696.4 0 386 80 244 696.4 0 244 81 70 696.4 0 70 82 21 696.4 0 21 83 20 696.4 0 20 84 28 696.4 0 28 85 24 696.4 0 24 86 25 696.4 0 25 87 36 696.4 0 36 88 26 696.4 0 26 89 29 696.4 0 29 90 30 696.4 0 30 91 16 696.4 0 16 92 19 696.4 0 19 93 25 696.4 0 25 94 20 696.4 0 20 95 24 696.4 0 24 96 28 696.4 0 28 97 24 696.4 0 24 98 35 696.4 0 35 99 42 696.4 0 42 100 43 696.4 0 43 101 37 696.4 0 37 102 26 696.4 0 26 103 20 696.4 0 20 104 21 696.4 0 21 105 21 696.4 0 21 106 24 696.4 0 24 107 32 696.4 0 32 108 16 696.4 0 16 109 27 696.4 0 27 110 29 696.4 0 29 111 26 696.4 0 26 112 60 696.4 0 60 113 77 696.4 0 77 114 77 696.4 0 77 115 25 696.4 0 25 116 36 696.4 0 36 117 188 696.4 0 188 118 85 696.4 0 85 119 95 696.4 0 95 120 31 696.4 0 31 121 32 696.4 0 32 122 34 696.4 0 34 123 29 696.4 0 29 124 33 696.4 0 33 125 32 696.4 0 32 126 63 696.4 0 63 127 31 696.4 0 31 128 75 696.4 0 75 129 46 696.4 0 46 130 61 696.4 0 61 131 357 696.4 0 357 132 149 696.4 0 149 133 227 696.4 0 227 134 76 696.4 0 76 135 39 696.4 0 39 136 75 696.4 0 75 137 49 696.4 0 49 138 164 696.4 0 164 139 68 696.4 0 68 140 67 696.4 0 67 141 23 696.4 0 23 142 29 696.4 0 29 143 37 696.4 0 37 144 71 696.4 0 71 145 95 696.4 0 95 146 107 696.4 0 107 147 147 696.4 0 147 148 39 696.4 0 39 149 34 696.4 0 34 150 26 696.4 0 26 151 23 696.4 0 23 152 20 696.4 0 20 153 27 696.4 0 27 154 42 696.4 0 42 155 37 696.4 0 37 156 42 696.4 0 42 157 71 696.4 0 71 158 55 696.4 0 55 159 44 696.4 0 44 160 36 696.4 0 36 161 54 696.4 0 54 162 46 696.4 0 46 163 47 696.4 0 47 164 53 696.4 0 53 165 78 696.4 0 78 166 65 696.4 0 65 167 78 696.4 0 78 168 93 696.4 0 93 169 63 696.4 0 63 170 50 696.4 0 50 171 70 696.4 0 70 172 61 696.4 0 61 173 183 696.4 0 183 174 73 696.4 0 73 175 92 696.4 0 92 176 392 696.4 0 392 177 414 696.4 0 414 178 2478 696.4 0 2478 179 1399 696.4 0 1399 180 2611 696.4 0 2611 181 1407 696.4 0 1407 182 1441 696.4 0 1441 183 669 696.4 0 669 184 222 696.4 0 222 185 63 696.4 0 63 186 34 696.4 0 34 187 53 696.4 0 53 188 44 696.4 0 44 189 72 696.4 0 72 190 47 696.4 0 47 191 36 696.4 0 36 192 56 696.4 0 56 193 60 696.4 0 60 194 121 696.4 0 121 195 225 696.4 0 225 196 788 696.4 0 788 197 1123 696.4 0 1123 198 489 696.4 0 489 199 163 696.4 0 163 200 69 696.4 0 69 201 236 696.4 0 236 202 492 696.4 0 492 203 370 696.4 0 370 204 222 696.4 0 222 205 23 696.4 0 23 206 38 696.4 0 38 207 33 696.4 0 33 208 34 696.4 0 34 209 41 696.4 0 41 210 35 696.4 0 35 211 21 696.4 0 21 212 31 696.4 0 31 213 32 696.4 0 32 214 44 696.4 0 44 215 133 696.4 0 133 216 149 696.4 0 149 217 125 696.4 0 125 218 37 696.4 0 37 219 34 696.4 0 34 220 35 696.4 0 35 221 86 696.4 0 86 222 249 696.4 0 249 223 508 696.4 0 508 224 328 696.4 0 328 225 310 696.4 0 310 226 32 696.4 0 32 227 27 696.4 0 27 228 46 696.4 0 46 229 32 696.4 0 32 230 37 696.4 0 37 231 44 696.4 0 44 232 42 696.4 0 42 233 59 696.4 0 59 234 402 696.4 0 402 235 173 696.4 0 173 236 298 696.4 0 298 237 38 696.4 0 38 238 52 696.4 0 52 239 269 696.4 0 269 240 256 696.4 0 256 241 298 696.4 0 298 242 181 696.4 0 181 243 166 696.4 0 166 244 96 696.4 0 96 245 66 696.4 0 66 246 53 696.4 0 53 247 46 696.4 0 46 248 36 696.4 0 36 249 52 696.4 0 52 250 38 696.4 0 38 251 68 696.4 0 68 252 80 696.4 0 80 253 75 696.4 0 75 254 151 696.4 0 151 255 76 696.4 0 76 256 78 696.4 0 78 257 300 696.4 0 300 258 296 696.4 0 296 259 1165 696.4 0 1165 260 797 696.4 0 797 261 1025 696.4 0 1025 262 166 696.4 0 166 263 181 696.4 0 181 264 836 696.4 0 836 265 1525 696.4 0 1525 266 1156 696.4 0 1156 267 668 696.4 0 668 268 24 696.4 0 24 269 23 696.4 0 23 270 29 696.4 0 29 271 45 696.4 0 45 272 32 696.4 0 32 273 61 696.4 0 61 274 69 696.4 0 69 275 59 696.4 0 59 276 86 696.4 0 86 277 102 696.4 0 102 278 90 696.4 0 90 279 93 696.4 0 93 280 33 696.4 0 33 281 47 696.4 0 47 282 41 696.4 0 41 283 35 696.4 0 35 284 58 696.4 0 58 285 48 696.4 0 48 286 53 696.4 0 53 287 89 696.4 0 89 288 59 696.4 0 59 289 60 696.4 0 60 290 119 696.4 0 119 291 958 696.4 0 958 292 729 696.4 0 729 293 1112 696.4 0 1112 294 339 696.4 0 339 295 100 696.4 0 100 296 81 696.4 0 81 297 199 696.4 0 199 298 96 696.4 0 96 299 144 696.4 0 144 300 169 696.4 0 169 301 118 696.4 0 118 Finished in 4.39 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 94,489 Reads with adapters: 35,796 (37.9%) Reads that were too short: 35 (0.0%) Reads written (passing filters): 35,761 (37.8%) Total basepairs processed: 24,275,131 bp Total written (filtered): 9,859,926 bp (40.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 35796 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 10 0.0 3 0 0 9 1 16 247 0.0 3 0 243 4 17 31956 0.0 3 31203 718 30 5 18 76 0.0 3 1 69 6 19 58 0.0 3 5 26 23 4 20 3340 0.0 3 3258 61 5 16 21 15 0.0 3 1 14 22 3 0.0 3 0 0 2 1 23 3 0.0 3 0 0 0 3 27 11 0.0 3 1 0 0 10 28 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 35 6 0.0 3 0 0 1 5 36 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 0 2 48 2 0.0 3 0 0 0 2 50 3 0.0 3 0 0 0 3 51 2 0.0 3 0 0 0 2 54 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 57 3 0.0 3 0 0 0 3 60 9 0.0 3 0 0 0 9 68 1 0.0 3 0 0 1 71 1 0.0 3 0 0 0 1 74 7 0.0 3 0 0 0 7 76 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 3 0.0 3 0 0 0 3 92 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 3 0.0 3 0 0 0 3 130 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 144 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 183 2 0.0 3 0 1 0 1 184 2 0.0 3 1 0 0 1 189 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.27 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 35,761 Reads with adapters: 693 (1.9%) Reads written (passing filters): 35,761 (100.0%) Total basepairs processed: 9,859,926 bp Total written (filtered): 9,857,751 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 693 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 79.9% C: 6.2% G: 11.1% T: 2.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 622 558.8 0 622 4 59 139.7 0 59 5 9 34.9 0 9 6 1 8.7 0 1 11 2 0.0 1 0 2