This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.73 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 163,015 (5.7%) Reads that were too short: 83,847 (2.9%) Reads written (passing filters): 79,168 (2.8%) Total basepairs processed: 858,556,748 bp Total written (filtered): 21,691,145 bp (2.5%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 163015 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4336 696.4 0 4336 7 30263 696.4 0 30263 8 27837 696.4 0 27837 9 37 696.4 0 37 10 31 696.4 0 31 11 7 696.4 0 7 12 17 696.4 0 17 13 6 696.4 0 6 14 14 696.4 0 14 15 16 696.4 0 16 16 17 696.4 0 17 17 16 696.4 0 16 18 13 696.4 0 13 19 20 696.4 0 20 20 11 696.4 0 11 21 17 696.4 0 17 22 8 696.4 0 8 23 21 696.4 0 21 24 20 696.4 0 20 25 17 696.4 0 17 26 9 696.4 0 9 27 5 696.4 0 5 28 13 696.4 0 13 29 21 696.4 0 21 30 12 696.4 0 12 31 11 696.4 0 11 32 8 696.4 0 8 33 16 696.4 0 16 34 10 696.4 0 10 35 21 696.4 0 21 36 11 696.4 0 11 37 8 696.4 0 8 38 12 696.4 0 12 39 17 696.4 0 17 40 11 696.4 0 11 41 15 696.4 0 15 42 16 696.4 0 16 43 19 696.4 0 19 44 17 696.4 0 17 45 20 696.4 0 20 46 21 696.4 0 21 47 11 696.4 0 11 48 35 696.4 0 35 49 18 696.4 0 18 50 27 696.4 0 27 51 21 696.4 0 21 52 27 696.4 0 27 53 9 696.4 0 9 54 31 696.4 0 31 55 26 696.4 0 26 56 17 696.4 0 17 57 19 696.4 0 19 58 25 696.4 0 25 59 25 696.4 0 25 60 32 696.4 0 32 61 48 696.4 0 48 62 103 696.4 0 103 63 43 696.4 0 43 64 61 696.4 0 61 65 73 696.4 0 73 66 21 696.4 0 21 67 30 696.4 0 30 68 57 696.4 0 57 69 74 696.4 0 74 70 54 696.4 0 54 71 44 696.4 0 44 72 73 696.4 0 73 73 136 696.4 0 136 74 237 696.4 0 237 75 3965 696.4 0 3965 76 2384 696.4 0 2384 77 3198 696.4 0 3198 78 24 696.4 0 24 79 13 696.4 0 13 80 9 696.4 0 9 81 18 696.4 0 18 82 16 696.4 0 16 83 17 696.4 0 17 84 14 696.4 0 14 85 12 696.4 0 12 86 9 696.4 0 9 87 19 696.4 0 19 88 21 696.4 0 21 89 16 696.4 0 16 90 14 696.4 0 14 91 32 696.4 0 32 92 16 696.4 0 16 93 22 696.4 0 22 94 21 696.4 0 21 95 45 696.4 0 45 96 16 696.4 0 16 97 17 696.4 0 17 98 32 696.4 0 32 99 9 696.4 0 9 100 10 696.4 0 10 101 22 696.4 0 22 102 23 696.4 0 23 103 22 696.4 0 22 104 19 696.4 0 19 105 25 696.4 0 25 106 19 696.4 0 19 107 7 696.4 0 7 108 24 696.4 0 24 109 22 696.4 0 22 110 22 696.4 0 22 111 17 696.4 0 17 112 25 696.4 0 25 113 16 696.4 0 16 114 24 696.4 0 24 115 23 696.4 0 23 116 18 696.4 0 18 117 24 696.4 0 24 118 22 696.4 0 22 119 19 696.4 0 19 120 16 696.4 0 16 121 23 696.4 0 23 122 18 696.4 0 18 123 24 696.4 0 24 124 23 696.4 0 23 125 150 696.4 0 150 126 126 696.4 0 126 127 89 696.4 0 89 128 21 696.4 0 21 129 35 696.4 0 35 130 22 696.4 0 22 131 13 696.4 0 13 132 42 696.4 0 42 133 27 696.4 0 27 134 30 696.4 0 30 135 34 696.4 0 34 136 35 696.4 0 35 137 34 696.4 0 34 138 55 696.4 0 55 139 37 696.4 0 37 140 17 696.4 0 17 141 20 696.4 0 20 142 25 696.4 0 25 143 31 696.4 0 31 144 11 696.4 0 11 145 19 696.4 0 19 146 38 696.4 0 38 147 13 696.4 0 13 148 13 696.4 0 13 149 16 696.4 0 16 150 37 696.4 0 37 151 19 696.4 0 19 152 13 696.4 0 13 153 16 696.4 0 16 154 26 696.4 0 26 155 18 696.4 0 18 156 24 696.4 0 24 157 14 696.4 0 14 158 28 696.4 0 28 159 16 696.4 0 16 160 21 696.4 0 21 161 29 696.4 0 29 162 17 696.4 0 17 163 39 696.4 0 39 164 29 696.4 0 29 165 23 696.4 0 23 166 35 696.4 0 35 167 68 696.4 0 68 168 94 696.4 0 94 169 58 696.4 0 58 170 45 696.4 0 45 171 30 696.4 0 30 172 27 696.4 0 27 173 26 696.4 0 26 174 20 696.4 0 20 175 16 696.4 0 16 176 27 696.4 0 27 177 20 696.4 0 20 178 23 696.4 0 23 179 26 696.4 0 26 180 26 696.4 0 26 181 25 696.4 0 25 182 32 696.4 0 32 183 35 696.4 0 35 184 42 696.4 0 42 185 29 696.4 0 29 186 83 696.4 0 83 187 69 696.4 0 69 188 94 696.4 0 94 189 69 696.4 0 69 190 30 696.4 0 30 191 39 696.4 0 39 192 56 696.4 0 56 193 178 696.4 0 178 194 128 696.4 0 128 195 117 696.4 0 117 196 109 696.4 0 109 197 481 696.4 0 481 198 326 696.4 0 326 199 315 696.4 0 315 200 115 696.4 0 115 201 73 696.4 0 73 202 46 696.4 0 46 203 106 696.4 0 106 204 99 696.4 0 99 205 77 696.4 0 77 206 35 696.4 0 35 207 27 696.4 0 27 208 27 696.4 0 27 209 19 696.4 0 19 210 39 696.4 0 39 211 69 696.4 0 69 212 36 696.4 0 36 213 41 696.4 0 41 214 64 696.4 0 64 215 45 696.4 0 45 216 47 696.4 0 47 217 122 696.4 0 122 218 223 696.4 0 223 219 135 696.4 0 135 220 130 696.4 0 130 221 72 696.4 0 72 222 40 696.4 0 40 223 86 696.4 0 86 224 179 696.4 0 179 225 79 696.4 0 79 226 63 696.4 0 63 227 40 696.4 0 40 228 56 696.4 0 56 229 38 696.4 0 38 230 52 696.4 0 52 231 87 696.4 0 87 232 97 696.4 0 97 233 155 696.4 0 155 234 259 696.4 0 259 235 439 696.4 0 439 236 600 696.4 0 600 237 663 696.4 0 663 238 9409 696.4 0 9409 239 6264 696.4 0 6264 240 5804 696.4 0 5804 241 176 696.4 0 176 242 223 696.4 0 223 243 271 696.4 0 271 244 222 696.4 0 222 245 249 696.4 0 249 246 384 696.4 0 384 247 3714 696.4 0 3714 248 3639 696.4 0 3639 249 11464 696.4 0 11464 250 13960 696.4 0 13960 251 7714 696.4 0 7714 252 71 696.4 0 71 253 52 696.4 0 52 254 45 696.4 0 45 255 26 696.4 0 26 256 19 696.4 0 19 257 28 696.4 0 28 258 46 696.4 0 46 259 86 696.4 0 86 260 21 696.4 0 21 261 23 696.4 0 23 262 34 696.4 0 34 263 65 696.4 0 65 264 40 696.4 0 40 265 54 696.4 0 54 266 33 696.4 0 33 267 49 696.4 0 49 268 99 696.4 0 99 269 124 696.4 0 124 270 68 696.4 0 68 271 120 696.4 0 120 272 123 696.4 0 123 273 74 696.4 0 74 274 40 696.4 0 40 275 91 696.4 0 91 276 76 696.4 0 76 277 265 696.4 0 265 278 152 696.4 0 152 279 410 696.4 0 410 280 420 696.4 0 420 281 469 696.4 0 469 282 1825 696.4 0 1825 283 1583 696.4 0 1583 284 4142 696.4 0 4142 285 1069 696.4 0 1069 286 238 696.4 0 238 287 201 696.4 0 201 288 104 696.4 0 104 289 56 696.4 0 56 290 59 696.4 0 59 291 76 696.4 0 76 292 70 696.4 0 70 293 68 696.4 0 68 294 424 696.4 0 424 295 655 696.4 0 655 296 537 696.4 0 537 297 660 696.4 0 660 298 189 696.4 0 189 299 304 696.4 0 304 300 327 696.4 0 327 301 152 696.4 0 152 Finished in 3.64 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 79,168 Reads with adapters: 28,355 (35.8%) Reads that were too short: 35 (0.0%) Reads written (passing filters): 28,320 (35.8%) Total basepairs processed: 21,691,145 bp Total written (filtered): 7,817,096 bp (36.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 28355 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 7 0.0 3 0 0 7 16 190 0.0 3 0 184 6 17 27810 0.0 3 27208 583 19 18 92 0.0 3 4 83 4 1 19 20 0.0 3 3 0 15 2 20 25 0.0 3 0 1 0 24 29 1 0.0 3 0 0 0 1 35 1 0.0 3 1 47 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 4 0.0 3 0 0 0 4 86 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 91 8 0.0 3 0 0 0 8 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 96 32 0.0 3 0 0 0 32 98 6 0.0 3 0 0 0 6 99 5 0.0 3 0 0 0 5 103 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 4 0.0 3 0 0 0 4 116 66 0.0 3 0 0 0 66 117 21 0.0 3 0 0 0 21 119 10 0.0 3 0 0 0 10 120 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 206 2 0.0 3 0 0 0 2 212 2 0.0 3 0 0 0 2 217 2 0.0 3 0 0 0 2 218 3 0.0 3 0 0 0 3 219 1 0.0 3 0 0 0 1 226 3 0.0 3 0 0 0 3 231 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.98 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 28,320 Reads with adapters: 75 (0.3%) Reads written (passing filters): 28,320 (100.0%) Total basepairs processed: 7,817,096 bp Total written (filtered): 7,816,840 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 75 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 45.3% C: 25.3% G: 26.7% T: 2.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 57 442.5 0 57 4 12 110.6 0 12 5 2 27.7 0 2 6 3 6.9 0 3 9 1 0.1 0 0 1