This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.57 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 114,221 (4.0%) Reads that were too short: 33,806 (1.2%) Reads written (passing filters): 80,415 (2.8%) Total basepairs processed: 858,556,748 bp Total written (filtered): 17,127,901 bp (2.0%) === Adapter 1 === Sequence: AAGACA; Type: regular 5'; Length: 6; Trimmed: 114221 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 775 696.4 0 775 7 15499 696.4 0 15499 8 423 696.4 0 423 9 16670 696.4 0 16670 10 58 696.4 0 58 11 14 696.4 0 14 12 26 696.4 0 26 13 9 696.4 0 9 14 8 696.4 0 8 15 16 696.4 0 16 16 11 696.4 0 11 17 15 696.4 0 15 18 11 696.4 0 11 19 6 696.4 0 6 20 17 696.4 0 17 21 7 696.4 0 7 22 6 696.4 0 6 23 13 696.4 0 13 24 26 696.4 0 26 25 14 696.4 0 14 26 8 696.4 0 8 27 10 696.4 0 10 28 9 696.4 0 9 29 17 696.4 0 17 30 19 696.4 0 19 31 10 696.4 0 10 32 11 696.4 0 11 33 16 696.4 0 16 34 16 696.4 0 16 35 21 696.4 0 21 36 45 696.4 0 45 37 12 696.4 0 12 38 12 696.4 0 12 39 13 696.4 0 13 40 21 696.4 0 21 41 34 696.4 0 34 42 24 696.4 0 24 43 29 696.4 0 29 44 8 696.4 0 8 45 13 696.4 0 13 46 14 696.4 0 14 47 9 696.4 0 9 48 21 696.4 0 21 49 13 696.4 0 13 50 13 696.4 0 13 51 22 696.4 0 22 52 21 696.4 0 21 53 19 696.4 0 19 54 92 696.4 0 92 55 28 696.4 0 28 56 34 696.4 0 34 57 11 696.4 0 11 58 12 696.4 0 12 59 14 696.4 0 14 60 16 696.4 0 16 61 11 696.4 0 11 62 25 696.4 0 25 63 32 696.4 0 32 64 89 696.4 0 89 65 73 696.4 0 73 66 46 696.4 0 46 67 28 696.4 0 28 68 26 696.4 0 26 69 40 696.4 0 40 70 52 696.4 0 52 71 430 696.4 0 430 72 301 696.4 0 301 73 486 696.4 0 486 74 464 696.4 0 464 75 213 696.4 0 213 76 357 696.4 0 357 77 237 696.4 0 237 78 129 696.4 0 129 79 295 696.4 0 295 80 146 696.4 0 146 81 83 696.4 0 83 82 154 696.4 0 154 83 261 696.4 0 261 84 167 696.4 0 167 85 84 696.4 0 84 86 117 696.4 0 117 87 66 696.4 0 66 88 73 696.4 0 73 89 112 696.4 0 112 90 41 696.4 0 41 91 36 696.4 0 36 92 64 696.4 0 64 93 51 696.4 0 51 94 75 696.4 0 75 95 67 696.4 0 67 96 54 696.4 0 54 97 42 696.4 0 42 98 54 696.4 0 54 99 88 696.4 0 88 100 48 696.4 0 48 101 101 696.4 0 101 102 208 696.4 0 208 103 203 696.4 0 203 104 199 696.4 0 199 105 290 696.4 0 290 106 193 696.4 0 193 107 927 696.4 0 927 108 814 696.4 0 814 109 971 696.4 0 971 110 299 696.4 0 299 111 251 696.4 0 251 112 265 696.4 0 265 113 249 696.4 0 249 114 211 696.4 0 211 115 244 696.4 0 244 116 266 696.4 0 266 117 413 696.4 0 413 118 337 696.4 0 337 119 316 696.4 0 316 120 559 696.4 0 559 121 507 696.4 0 507 122 751 696.4 0 751 123 451 696.4 0 451 124 305 696.4 0 305 125 402 696.4 0 402 126 284 696.4 0 284 127 194 696.4 0 194 128 211 696.4 0 211 129 224 696.4 0 224 130 191 696.4 0 191 131 239 696.4 0 239 132 168 696.4 0 168 133 186 696.4 0 186 134 204 696.4 0 204 135 137 696.4 0 137 136 185 696.4 0 185 137 208 696.4 0 208 138 250 696.4 0 250 139 242 696.4 0 242 140 190 696.4 0 190 141 394 696.4 0 394 142 211 696.4 0 211 143 341 696.4 0 341 144 201 696.4 0 201 145 232 696.4 0 232 146 226 696.4 0 226 147 284 696.4 0 284 148 226 696.4 0 226 149 288 696.4 0 288 150 244 696.4 0 244 151 276 696.4 0 276 152 239 696.4 0 239 153 203 696.4 0 203 154 161 696.4 0 161 155 145 696.4 0 145 156 171 696.4 0 171 157 172 696.4 0 172 158 130 696.4 0 130 159 166 696.4 0 166 160 191 696.4 0 191 161 224 696.4 0 224 162 195 696.4 0 195 163 170 696.4 0 170 164 243 696.4 0 243 165 267 696.4 0 267 166 1460 696.4 0 1460 167 1626 696.4 0 1626 168 1047 696.4 0 1047 169 399 696.4 0 399 170 2426 696.4 0 2426 171 1461 696.4 0 1461 172 1938 696.4 0 1938 173 224 696.4 0 224 174 182 696.4 0 182 175 159 696.4 0 159 176 258 696.4 0 258 177 192 696.4 0 192 178 160 696.4 0 160 179 366 696.4 0 366 180 340 696.4 0 340 181 2003 696.4 0 2003 182 1407 696.4 0 1407 183 1509 696.4 0 1509 184 804 696.4 0 804 185 717 696.4 0 717 186 391 696.4 0 391 187 163 696.4 0 163 188 175 696.4 0 175 189 168 696.4 0 168 190 145 696.4 0 145 191 138 696.4 0 138 192 167 696.4 0 167 193 135 696.4 0 135 194 148 696.4 0 148 195 152 696.4 0 152 196 185 696.4 0 185 197 152 696.4 0 152 198 465 696.4 0 465 199 538 696.4 0 538 200 388 696.4 0 388 201 254 696.4 0 254 202 170 696.4 0 170 203 197 696.4 0 197 204 323 696.4 0 323 205 444 696.4 0 444 206 452 696.4 0 452 207 345 696.4 0 345 208 172 696.4 0 172 209 147 696.4 0 147 210 182 696.4 0 182 211 231 696.4 0 231 212 281 696.4 0 281 213 1641 696.4 0 1641 214 1983 696.4 0 1983 215 1457 696.4 0 1457 216 226 696.4 0 226 217 211 696.4 0 211 218 226 696.4 0 226 219 249 696.4 0 249 220 218 696.4 0 218 221 139 696.4 0 139 222 147 696.4 0 147 223 153 696.4 0 153 224 207 696.4 0 207 225 523 696.4 0 523 226 495 696.4 0 495 227 531 696.4 0 531 228 351 696.4 0 351 229 345 696.4 0 345 230 299 696.4 0 299 231 153 696.4 0 153 232 127 696.4 0 127 233 153 696.4 0 153 234 113 696.4 0 113 235 144 696.4 0 144 236 113 696.4 0 113 237 309 696.4 0 309 238 273 696.4 0 273 239 571 696.4 0 571 240 193 696.4 0 193 241 153 696.4 0 153 242 205 696.4 0 205 243 212 696.4 0 212 244 233 696.4 0 233 245 310 696.4 0 310 246 380 696.4 0 380 247 306 696.4 0 306 248 233 696.4 0 233 249 181 696.4 0 181 250 196 696.4 0 196 251 159 696.4 0 159 252 155 696.4 0 155 253 175 696.4 0 175 254 342 696.4 0 342 255 361 696.4 0 361 256 233 696.4 0 233 257 239 696.4 0 239 258 117 696.4 0 117 259 141 696.4 0 141 260 228 696.4 0 228 261 280 696.4 0 280 262 503 696.4 0 503 263 369 696.4 0 369 264 311 696.4 0 311 265 211 696.4 0 211 266 262 696.4 0 262 267 1170 696.4 0 1170 268 1215 696.4 0 1215 269 1341 696.4 0 1341 270 335 696.4 0 335 271 139 696.4 0 139 272 124 696.4 0 124 273 147 696.4 0 147 274 152 696.4 0 152 275 193 696.4 0 193 276 262 696.4 0 262 277 329 696.4 0 329 278 245 696.4 0 245 279 384 696.4 0 384 280 1429 696.4 0 1429 281 869 696.4 0 869 282 973 696.4 0 973 283 207 696.4 0 207 284 109 696.4 0 109 285 133 696.4 0 133 286 165 696.4 0 165 287 148 696.4 0 148 288 172 696.4 0 172 289 150 696.4 0 150 290 162 696.4 0 162 291 118 696.4 0 118 292 124 696.4 0 124 293 168 696.4 0 168 294 149 696.4 0 149 295 143 696.4 0 143 296 118 696.4 0 118 297 137 696.4 0 137 298 156 696.4 0 156 299 263 696.4 0 263 300 330 696.4 0 330 301 558 696.4 0 558 Finished in 3.50 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 80,415 Reads with adapters: 16,015 (19.9%) Reads that were too short: 57 (0.1%) Reads written (passing filters): 15,958 (19.8%) Total basepairs processed: 17,127,901 bp Total written (filtered): 4,365,196 bp (25.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 16015 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 11 0.0 3 0 0 7 4 19 187 0.0 3 0 160 18 9 20 15624 0.0 4 14916 574 101 23 10 21 40 0.0 4 6 31 2 0 1 22 6 0.0 4 2 0 3 0 1 23 13 0.0 4 0 0 0 9 4 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 1 27 2 0.0 4 0 0 0 0 2 30 1 0.0 4 0 0 0 0 1 31 3 0.0 4 1 0 1 0 1 33 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 39 1 0.0 4 1 42 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 48 2 0.0 4 0 0 0 0 2 49 1 0.0 4 0 0 0 0 1 50 2 0.0 4 0 0 0 0 2 53 2 0.0 4 0 0 0 0 2 54 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 70 2 0.0 4 0 0 0 0 2 71 2 0.0 4 0 0 0 1 1 72 2 0.0 4 0 0 0 0 2 74 2 0.0 4 0 0 0 0 2 75 2 0.0 4 0 0 0 0 2 76 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 91 3 0.0 4 0 0 0 0 3 93 1 0.0 4 0 0 0 0 1 95 2 0.0 4 0 0 0 0 2 98 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 122 3 0.0 4 0 0 0 0 3 123 1 0.0 4 1 124 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 1 0.0 4 0 0 0 0 1 127 43 0.0 4 0 0 0 0 43 129 1 0.0 4 0 0 0 0 1 137 1 0.0 4 1 138 1 0.0 4 1 147 2 0.0 4 0 0 0 0 2 151 3 0.0 4 0 0 0 0 3 153 1 0.0 4 0 0 0 0 1 160 1 0.0 4 1 170 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 244 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.57 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 15,958 Reads with adapters: 1,195 (7.5%) Reads written (passing filters): 15,958 (100.0%) Total basepairs processed: 4,365,196 bp Total written (filtered): 4,343,584 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1195 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 15.6% C: 14.4% G: 66.3% T: 3.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 66 249.3 0 66 4 7 62.3 0 7 7 6 1.0 0 6 8 1 0.2 0 1 9 2 0.1 0 0 2 10 13 0.0 1 1 12 11 699 0.0 1 41 658 12 204 0.0 1 9 195 13 8 0.0 1 2 6 14 2 0.0 1 0 2 15 2 0.0 1 0 2 20 12 0.0 1 5 7 21 116 0.0 1 79 37 22 7 0.0 1 4 3 23 7 0.0 1 4 3 24 2 0.0 1 1 1 25 1 0.0 1 0 1 79 1 0.0 1 1 123 1 0.0 1 0 1 152 13 0.0 1 9 4 210 1 0.0 1 1 225 23 0.0 1 21 2 262 1 0.0 1 0 1