This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.09 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 101,731 (3.6%) Reads that were too short: 27,285 (1.0%) Reads written (passing filters): 74,446 (2.6%) Total basepairs processed: 858,556,748 bp Total written (filtered): 16,387,541 bp (1.9%) === Adapter 1 === Sequence: AAGACA; Type: regular 5'; Length: 6; Trimmed: 101731 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 426 696.4 0 426 7 18163 696.4 0 18163 8 244 696.4 0 244 9 15685 696.4 0 15685 10 15 696.4 0 15 11 32 696.4 0 32 12 24 696.4 0 24 13 19 696.4 0 19 14 20 696.4 0 20 15 20 696.4 0 20 16 13 696.4 0 13 17 17 696.4 0 17 18 20 696.4 0 20 19 13 696.4 0 13 20 31 696.4 0 31 21 5 696.4 0 5 22 10 696.4 0 10 23 17 696.4 0 17 24 12 696.4 0 12 25 13 696.4 0 13 26 20 696.4 0 20 27 21 696.4 0 21 28 20 696.4 0 20 29 31 696.4 0 31 30 21 696.4 0 21 31 23 696.4 0 23 32 22 696.4 0 22 33 22 696.4 0 22 34 23 696.4 0 23 35 22 696.4 0 22 36 59 696.4 0 59 37 22 696.4 0 22 38 13 696.4 0 13 39 13 696.4 0 13 40 15 696.4 0 15 41 46 696.4 0 46 42 33 696.4 0 33 43 64 696.4 0 64 44 18 696.4 0 18 45 20 696.4 0 20 46 13 696.4 0 13 47 15 696.4 0 15 48 20 696.4 0 20 49 11 696.4 0 11 50 24 696.4 0 24 51 24 696.4 0 24 52 15 696.4 0 15 53 19 696.4 0 19 54 35 696.4 0 35 55 32 696.4 0 32 56 34 696.4 0 34 57 23 696.4 0 23 58 15 696.4 0 15 59 20 696.4 0 20 60 16 696.4 0 16 61 19 696.4 0 19 62 18 696.4 0 18 63 12 696.4 0 12 64 33 696.4 0 33 65 33 696.4 0 33 66 21 696.4 0 21 67 11 696.4 0 11 68 23 696.4 0 23 69 30 696.4 0 30 70 29 696.4 0 29 71 26 696.4 0 26 72 57 696.4 0 57 73 67 696.4 0 67 74 255 696.4 0 255 75 168 696.4 0 168 76 225 696.4 0 225 77 167 696.4 0 167 78 234 696.4 0 234 79 265 696.4 0 265 80 393 696.4 0 393 81 243 696.4 0 243 82 262 696.4 0 262 83 394 696.4 0 394 84 303 696.4 0 303 85 150 696.4 0 150 86 178 696.4 0 178 87 284 696.4 0 284 88 200 696.4 0 200 89 129 696.4 0 129 90 96 696.4 0 96 91 109 696.4 0 109 92 88 696.4 0 88 93 89 696.4 0 89 94 74 696.4 0 74 95 59 696.4 0 59 96 83 696.4 0 83 97 101 696.4 0 101 98 69 696.4 0 69 99 85 696.4 0 85 100 100 696.4 0 100 101 139 696.4 0 139 102 163 696.4 0 163 103 149 696.4 0 149 104 173 696.4 0 173 105 204 696.4 0 204 106 177 696.4 0 177 107 923 696.4 0 923 108 791 696.4 0 791 109 962 696.4 0 962 110 200 696.4 0 200 111 197 696.4 0 197 112 198 696.4 0 198 113 151 696.4 0 151 114 162 696.4 0 162 115 155 696.4 0 155 116 191 696.4 0 191 117 334 696.4 0 334 118 284 696.4 0 284 119 369 696.4 0 369 120 283 696.4 0 283 121 311 696.4 0 311 122 483 696.4 0 483 123 253 696.4 0 253 124 236 696.4 0 236 125 226 696.4 0 226 126 168 696.4 0 168 127 140 696.4 0 140 128 157 696.4 0 157 129 240 696.4 0 240 130 159 696.4 0 159 131 170 696.4 0 170 132 157 696.4 0 157 133 154 696.4 0 154 134 129 696.4 0 129 135 126 696.4 0 126 136 142 696.4 0 142 137 198 696.4 0 198 138 178 696.4 0 178 139 180 696.4 0 180 140 159 696.4 0 159 141 216 696.4 0 216 142 163 696.4 0 163 143 270 696.4 0 270 144 155 696.4 0 155 145 159 696.4 0 159 146 191 696.4 0 191 147 222 696.4 0 222 148 159 696.4 0 159 149 182 696.4 0 182 150 158 696.4 0 158 151 240 696.4 0 240 152 185 696.4 0 185 153 163 696.4 0 163 154 124 696.4 0 124 155 149 696.4 0 149 156 165 696.4 0 165 157 167 696.4 0 167 158 193 696.4 0 193 159 142 696.4 0 142 160 171 696.4 0 171 161 209 696.4 0 209 162 165 696.4 0 165 163 172 696.4 0 172 164 177 696.4 0 177 165 219 696.4 0 219 166 1294 696.4 0 1294 167 1377 696.4 0 1377 168 953 696.4 0 953 169 269 696.4 0 269 170 1211 696.4 0 1211 171 892 696.4 0 892 172 1095 696.4 0 1095 173 161 696.4 0 161 174 171 696.4 0 171 175 154 696.4 0 154 176 267 696.4 0 267 177 210 696.4 0 210 178 211 696.4 0 211 179 357 696.4 0 357 180 376 696.4 0 376 181 1984 696.4 0 1984 182 1352 696.4 0 1352 183 1418 696.4 0 1418 184 816 696.4 0 816 185 828 696.4 0 828 186 391 696.4 0 391 187 157 696.4 0 157 188 183 696.4 0 183 189 157 696.4 0 157 190 118 696.4 0 118 191 144 696.4 0 144 192 157 696.4 0 157 193 132 696.4 0 132 194 150 696.4 0 150 195 153 696.4 0 153 196 155 696.4 0 155 197 172 696.4 0 172 198 394 696.4 0 394 199 506 696.4 0 506 200 347 696.4 0 347 201 222 696.4 0 222 202 157 696.4 0 157 203 142 696.4 0 142 204 172 696.4 0 172 205 289 696.4 0 289 206 229 696.4 0 229 207 196 696.4 0 196 208 152 696.4 0 152 209 137 696.4 0 137 210 164 696.4 0 164 211 196 696.4 0 196 212 259 696.4 0 259 213 1874 696.4 0 1874 214 2278 696.4 0 2278 215 1818 696.4 0 1818 216 270 696.4 0 270 217 168 696.4 0 168 218 184 696.4 0 184 219 180 696.4 0 180 220 204 696.4 0 204 221 156 696.4 0 156 222 130 696.4 0 130 223 129 696.4 0 129 224 175 696.4 0 175 225 260 696.4 0 260 226 280 696.4 0 280 227 250 696.4 0 250 228 322 696.4 0 322 229 311 696.4 0 311 230 268 696.4 0 268 231 127 696.4 0 127 232 110 696.4 0 110 233 142 696.4 0 142 234 118 696.4 0 118 235 107 696.4 0 107 236 120 696.4 0 120 237 217 696.4 0 217 238 194 696.4 0 194 239 516 696.4 0 516 240 131 696.4 0 131 241 139 696.4 0 139 242 145 696.4 0 145 243 138 696.4 0 138 244 187 696.4 0 187 245 154 696.4 0 154 246 174 696.4 0 174 247 162 696.4 0 162 248 171 696.4 0 171 249 152 696.4 0 152 250 171 696.4 0 171 251 160 696.4 0 160 252 144 696.4 0 144 253 170 696.4 0 170 254 234 696.4 0 234 255 208 696.4 0 208 256 202 696.4 0 202 257 202 696.4 0 202 258 104 696.4 0 104 259 126 696.4 0 126 260 144 696.4 0 144 261 185 696.4 0 185 262 295 696.4 0 295 263 245 696.4 0 245 264 227 696.4 0 227 265 151 696.4 0 151 266 222 696.4 0 222 267 1259 696.4 0 1259 268 1356 696.4 0 1356 269 1424 696.4 0 1424 270 203 696.4 0 203 271 117 696.4 0 117 272 113 696.4 0 113 273 120 696.4 0 120 274 123 696.4 0 123 275 163 696.4 0 163 276 154 696.4 0 154 277 168 696.4 0 168 278 155 696.4 0 155 279 159 696.4 0 159 280 681 696.4 0 681 281 457 696.4 0 457 282 564 696.4 0 564 283 124 696.4 0 124 284 99 696.4 0 99 285 100 696.4 0 100 286 115 696.4 0 115 287 129 696.4 0 129 288 109 696.4 0 109 289 116 696.4 0 116 290 112 696.4 0 112 291 101 696.4 0 101 292 109 696.4 0 109 293 142 696.4 0 142 294 119 696.4 0 119 295 108 696.4 0 108 296 109 696.4 0 109 297 96 696.4 0 96 298 119 696.4 0 119 299 150 696.4 0 150 300 168 696.4 0 168 301 200 696.4 0 200 Finished in 3.16 s (42 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 74,446 Reads with adapters: 16,270 (21.9%) Reads that were too short: 116 (0.2%) Reads written (passing filters): 16,154 (21.7%) Total basepairs processed: 16,387,541 bp Total written (filtered): 4,429,195 bp (27.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 16270 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 103 0.0 3 0 101 2 17 15902 0.0 3 15568 315 18 1 18 37 0.0 3 4 32 1 19 14 0.0 3 0 0 10 4 20 12 0.0 3 0 0 0 12 21 44 0.0 3 1 0 0 43 24 6 0.0 3 0 0 0 6 25 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 28 2 0.0 3 0 0 0 2 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 3 0.0 3 0 0 0 3 49 1 0.0 3 0 0 0 1 51 5 0.0 3 0 0 0 5 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 71 7 0.0 3 0 0 0 7 75 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 110 1 0.0 3 1 114 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 122 4 0.0 3 0 0 0 4 123 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 207 1 0.0 3 0 1 209 2 0.0 3 0 0 0 2 217 78 0.0 3 0 0 0 78 219 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.65 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 16,154 Reads with adapters: 494 (3.1%) Reads written (passing filters): 16,154 (100.0%) Total basepairs processed: 4,429,195 bp Total written (filtered): 4,427,684 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 494 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.1% C: 6.5% G: 15.6% T: 1.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 475 252.4 0 475 4 13 63.1 0 13 5 2 15.8 0 2 6 4 3.9 0 4