This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.09 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 102,642 (3.6%) Reads that were too short: 20,134 (0.7%) Reads written (passing filters): 82,508 (2.9%) Total basepairs processed: 858,556,748 bp Total written (filtered): 19,555,331 bp (2.3%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 102642 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 694 696.4 0 694 7 2795 696.4 0 2795 8 23902 696.4 0 23902 9 21356 696.4 0 21356 10 116 696.4 0 116 11 30 696.4 0 30 12 36 696.4 0 36 13 24 696.4 0 24 14 48 696.4 0 48 15 19 696.4 0 19 16 26 696.4 0 26 17 31 696.4 0 31 18 30 696.4 0 30 19 28 696.4 0 28 20 37 696.4 0 37 21 36 696.4 0 36 22 19 696.4 0 19 23 37 696.4 0 37 24 24 696.4 0 24 25 36 696.4 0 36 26 27 696.4 0 27 27 30 696.4 0 30 28 34 696.4 0 34 29 70 696.4 0 70 30 33 696.4 0 33 31 30 696.4 0 30 32 26 696.4 0 26 33 33 696.4 0 33 34 28 696.4 0 28 35 60 696.4 0 60 36 59 696.4 0 59 37 44 696.4 0 44 38 37 696.4 0 37 39 54 696.4 0 54 40 54 696.4 0 54 41 38 696.4 0 38 42 38 696.4 0 38 43 41 696.4 0 41 44 30 696.4 0 30 45 30 696.4 0 30 46 41 696.4 0 41 47 26 696.4 0 26 48 34 696.4 0 34 49 44 696.4 0 44 50 36 696.4 0 36 51 35 696.4 0 35 52 49 696.4 0 49 53 63 696.4 0 63 54 73 696.4 0 73 55 40 696.4 0 40 56 40 696.4 0 40 57 37 696.4 0 37 58 67 696.4 0 67 59 67 696.4 0 67 60 38 696.4 0 38 61 39 696.4 0 39 62 34 696.4 0 34 63 92 696.4 0 92 64 115 696.4 0 115 65 64 696.4 0 64 66 38 696.4 0 38 67 65 696.4 0 65 68 92 696.4 0 92 69 78 696.4 0 78 70 54 696.4 0 54 71 84 696.4 0 84 72 458 696.4 0 458 73 1836 696.4 0 1836 74 1351 696.4 0 1351 75 658 696.4 0 658 76 42 696.4 0 42 77 29 696.4 0 29 78 32 696.4 0 32 79 61 696.4 0 61 80 33 696.4 0 33 81 31 696.4 0 31 82 32 696.4 0 32 83 45 696.4 0 45 84 64 696.4 0 64 85 64 696.4 0 64 86 93 696.4 0 93 87 92 696.4 0 92 88 270 696.4 0 270 89 88 696.4 0 88 90 63 696.4 0 63 91 52 696.4 0 52 92 98 696.4 0 98 93 46 696.4 0 46 94 43 696.4 0 43 95 60 696.4 0 60 96 40 696.4 0 40 97 28 696.4 0 28 98 38 696.4 0 38 99 55 696.4 0 55 100 75 696.4 0 75 101 50 696.4 0 50 102 40 696.4 0 40 103 29 696.4 0 29 104 40 696.4 0 40 105 67 696.4 0 67 106 42 696.4 0 42 107 41 696.4 0 41 108 81 696.4 0 81 109 69 696.4 0 69 110 73 696.4 0 73 111 47 696.4 0 47 112 31 696.4 0 31 113 41 696.4 0 41 114 34 696.4 0 34 115 55 696.4 0 55 116 33 696.4 0 33 117 36 696.4 0 36 118 41 696.4 0 41 119 32 696.4 0 32 120 35 696.4 0 35 121 35 696.4 0 35 122 43 696.4 0 43 123 32 696.4 0 32 124 46 696.4 0 46 125 52 696.4 0 52 126 57 696.4 0 57 127 59 696.4 0 59 128 95 696.4 0 95 129 90 696.4 0 90 130 107 696.4 0 107 131 53 696.4 0 53 132 49 696.4 0 49 133 60 696.4 0 60 134 60 696.4 0 60 135 99 696.4 0 99 136 108 696.4 0 108 137 110 696.4 0 110 138 60 696.4 0 60 139 40 696.4 0 40 140 43 696.4 0 43 141 75 696.4 0 75 142 72 696.4 0 72 143 53 696.4 0 53 144 126 696.4 0 126 145 225 696.4 0 225 146 334 696.4 0 334 147 557 696.4 0 557 148 293 696.4 0 293 149 151 696.4 0 151 150 129 696.4 0 129 151 52 696.4 0 52 152 51 696.4 0 51 153 98 696.4 0 98 154 106 696.4 0 106 155 271 696.4 0 271 156 212 696.4 0 212 157 361 696.4 0 361 158 653 696.4 0 653 159 442 696.4 0 442 160 465 696.4 0 465 161 99 696.4 0 99 162 194 696.4 0 194 163 92 696.4 0 92 164 63 696.4 0 63 165 144 696.4 0 144 166 207 696.4 0 207 167 376 696.4 0 376 168 161 696.4 0 161 169 128 696.4 0 128 170 171 696.4 0 171 171 156 696.4 0 156 172 140 696.4 0 140 173 201 696.4 0 201 174 2023 696.4 0 2023 175 1409 696.4 0 1409 176 917 696.4 0 917 177 146 696.4 0 146 178 85 696.4 0 85 179 70 696.4 0 70 180 148 696.4 0 148 181 159 696.4 0 159 182 356 696.4 0 356 183 146 696.4 0 146 184 161 696.4 0 161 185 389 696.4 0 389 186 494 696.4 0 494 187 3629 696.4 0 3629 188 3604 696.4 0 3604 189 1437 696.4 0 1437 190 68 696.4 0 68 191 59 696.4 0 59 192 74 696.4 0 74 193 56 696.4 0 56 194 64 696.4 0 64 195 69 696.4 0 69 196 50 696.4 0 50 197 64 696.4 0 64 198 60 696.4 0 60 199 65 696.4 0 65 200 93 696.4 0 93 201 98 696.4 0 98 202 108 696.4 0 108 203 170 696.4 0 170 204 177 696.4 0 177 205 192 696.4 0 192 206 123 696.4 0 123 207 80 696.4 0 80 208 56 696.4 0 56 209 116 696.4 0 116 210 65 696.4 0 65 211 44 696.4 0 44 212 50 696.4 0 50 213 56 696.4 0 56 214 105 696.4 0 105 215 94 696.4 0 94 216 77 696.4 0 77 217 57 696.4 0 57 218 75 696.4 0 75 219 82 696.4 0 82 220 112 696.4 0 112 221 94 696.4 0 94 222 72 696.4 0 72 223 107 696.4 0 107 224 80 696.4 0 80 225 70 696.4 0 70 226 63 696.4 0 63 227 145 696.4 0 145 228 139 696.4 0 139 229 93 696.4 0 93 230 93 696.4 0 93 231 121 696.4 0 121 232 145 696.4 0 145 233 99 696.4 0 99 234 92 696.4 0 92 235 122 696.4 0 122 236 158 696.4 0 158 237 152 696.4 0 152 238 152 696.4 0 152 239 114 696.4 0 114 240 116 696.4 0 116 241 66 696.4 0 66 242 76 696.4 0 76 243 71 696.4 0 71 244 98 696.4 0 98 245 105 696.4 0 105 246 116 696.4 0 116 247 251 696.4 0 251 248 331 696.4 0 331 249 451 696.4 0 451 250 475 696.4 0 475 251 776 696.4 0 776 252 799 696.4 0 799 253 474 696.4 0 474 254 199 696.4 0 199 255 133 696.4 0 133 256 103 696.4 0 103 257 100 696.4 0 100 258 129 696.4 0 129 259 165 696.4 0 165 260 398 696.4 0 398 261 919 696.4 0 919 262 1083 696.4 0 1083 263 645 696.4 0 645 264 245 696.4 0 245 265 90 696.4 0 90 266 67 696.4 0 67 267 88 696.4 0 88 268 162 696.4 0 162 269 228 696.4 0 228 270 172 696.4 0 172 271 159 696.4 0 159 272 172 696.4 0 172 273 167 696.4 0 167 274 159 696.4 0 159 275 122 696.4 0 122 276 104 696.4 0 104 277 150 696.4 0 150 278 209 696.4 0 209 279 253 696.4 0 253 280 165 696.4 0 165 281 383 696.4 0 383 282 378 696.4 0 378 283 251 696.4 0 251 284 145 696.4 0 145 285 251 696.4 0 251 286 212 696.4 0 212 287 109 696.4 0 109 288 145 696.4 0 145 289 134 696.4 0 134 290 276 696.4 0 276 291 387 696.4 0 387 292 377 696.4 0 377 293 237 696.4 0 237 294 175 696.4 0 175 295 260 696.4 0 260 296 193 696.4 0 193 297 269 696.4 0 269 298 334 696.4 0 334 299 345 696.4 0 345 300 258 696.4 0 258 301 274 696.4 0 274 Finished in 3.98 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 82,508 Reads with adapters: 23,592 (28.6%) Reads that were too short: 54 (0.1%) Reads written (passing filters): 23,538 (28.5%) Total basepairs processed: 19,555,331 bp Total written (filtered): 6,396,426 bp (32.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 23592 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 21 0.0 3 0 0 11 6 4 19 271 0.0 3 0 224 24 18 5 20 22818 0.0 4 21428 1125 199 45 21 21 67 0.0 4 10 51 5 0 1 22 9 0.0 4 0 3 4 0 2 23 272 0.0 4 138 7 1 94 32 29 1 0.0 4 1 32 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 1 43 3 0.0 4 1 0 0 0 2 48 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 56 3 0.0 4 0 0 0 0 3 57 2 0.0 4 0 0 0 0 2 61 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 64 0.0 4 0 0 0 0 64 131 2 0.0 4 0 0 0 0 2 136 1 0.0 4 0 0 0 1 153 2 0.0 4 0 0 0 0 2 155 13 0.0 4 0 0 0 0 13 167 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 1 204 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 1 0.0 4 0 0 0 0 1 236 2 0.0 4 0 0 0 0 2 237 2 0.0 4 0 0 0 0 2 247 1 0.0 4 0 0 1 250 1 0.0 4 1 260 1 0.0 4 0 0 0 0 1 264 2 0.0 4 0 0 0 0 2 265 3 0.0 4 0 0 0 1 2 266 1 0.0 4 0 0 0 0 1 267 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.90 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 23,538 Reads with adapters: 6,256 (26.6%) Reads written (passing filters): 23,538 (100.0%) Total basepairs processed: 6,396,426 bp Total written (filtered): 6,329,908 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6256 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.5% C: 7.8% G: 88.0% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 12 367.8 0 12 4 3 91.9 0 3 5 1 23.0 0 1 6 1 5.7 0 1 7 1 1.4 0 1 8 5 0.4 0 5 9 2423 0.1 0 250 2173 10 2546 0.0 1 195 2351 11 1144 0.0 1 53 1091 12 29 0.0 1 0 29 13 8 0.0 1 0 8 14 1 0.0 1 0 1 15 1 0.0 1 0 1 18 19 0.0 1 12 7 19 5 0.0 1 4 1 20 1 0.0 1 0 1 21 9 0.0 1 4 5 22 10 0.0 1 10 99 19 0.0 1 16 3 100 2 0.0 1 1 1 122 1 0.0 1 1 150 2 0.0 1 2 152 1 0.0 1 1 184 2 0.0 1 2 208 7 0.0 1 4 3 209 1 0.0 1 1 259 1 0.0 1 1 267 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.