This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.95 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 80,599 (2.8%) Reads that were too short: 20,684 (0.7%) Reads written (passing filters): 59,915 (2.1%) Total basepairs processed: 858,556,748 bp Total written (filtered): 13,837,540 bp (1.6%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 80599 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 763 696.4 0 763 7 16259 696.4 0 16259 8 14705 696.4 0 14705 9 39 696.4 0 39 10 66 696.4 0 66 11 238 696.4 0 238 12 220 696.4 0 220 13 440 696.4 0 440 14 491 696.4 0 491 15 391 696.4 0 391 16 25 696.4 0 25 17 16 696.4 0 16 18 8 696.4 0 8 19 12 696.4 0 12 20 8 696.4 0 8 21 15 696.4 0 15 22 11 696.4 0 11 23 27 696.4 0 27 24 40 696.4 0 40 25 147 696.4 0 147 26 178 696.4 0 178 27 65 696.4 0 65 28 12 696.4 0 12 29 14 696.4 0 14 30 18 696.4 0 18 31 22 696.4 0 22 32 16 696.4 0 16 33 17 696.4 0 17 34 18 696.4 0 18 35 18 696.4 0 18 36 20 696.4 0 20 37 13 696.4 0 13 38 24 696.4 0 24 39 16 696.4 0 16 40 14 696.4 0 14 41 14 696.4 0 14 42 17 696.4 0 17 43 15 696.4 0 15 44 22 696.4 0 22 45 18 696.4 0 18 46 13 696.4 0 13 47 23 696.4 0 23 48 24 696.4 0 24 49 14 696.4 0 14 50 19 696.4 0 19 51 16 696.4 0 16 52 21 696.4 0 21 53 30 696.4 0 30 54 18 696.4 0 18 55 29 696.4 0 29 56 19 696.4 0 19 57 37 696.4 0 37 58 23 696.4 0 23 59 21 696.4 0 21 60 38 696.4 0 38 61 70 696.4 0 70 62 38 696.4 0 38 63 21 696.4 0 21 64 13 696.4 0 13 65 14 696.4 0 14 66 28 696.4 0 28 67 24 696.4 0 24 68 13 696.4 0 13 69 18 696.4 0 18 70 25 696.4 0 25 71 30 696.4 0 30 72 29 696.4 0 29 73 27 696.4 0 27 74 40 696.4 0 40 75 35 696.4 0 35 76 47 696.4 0 47 77 37 696.4 0 37 78 306 696.4 0 306 79 60 696.4 0 60 80 77 696.4 0 77 81 84 696.4 0 84 82 505 696.4 0 505 83 470 696.4 0 470 84 298 696.4 0 298 85 27 696.4 0 27 86 34 696.4 0 34 87 50 696.4 0 50 88 23 696.4 0 23 89 42 696.4 0 42 90 34 696.4 0 34 91 62 696.4 0 62 92 24 696.4 0 24 93 35 696.4 0 35 94 59 696.4 0 59 95 63 696.4 0 63 96 51 696.4 0 51 97 78 696.4 0 78 98 41 696.4 0 41 99 22 696.4 0 22 100 38 696.4 0 38 101 44 696.4 0 44 102 70 696.4 0 70 103 67 696.4 0 67 104 71 696.4 0 71 105 67 696.4 0 67 106 59 696.4 0 59 107 59 696.4 0 59 108 36 696.4 0 36 109 32 696.4 0 32 110 98 696.4 0 98 111 200 696.4 0 200 112 75 696.4 0 75 113 81 696.4 0 81 114 56 696.4 0 56 115 55 696.4 0 55 116 49 696.4 0 49 117 30 696.4 0 30 118 68 696.4 0 68 119 88 696.4 0 88 120 126 696.4 0 126 121 73 696.4 0 73 122 272 696.4 0 272 123 191 696.4 0 191 124 138 696.4 0 138 125 41 696.4 0 41 126 49 696.4 0 49 127 74 696.4 0 74 128 68 696.4 0 68 129 57 696.4 0 57 130 53 696.4 0 53 131 59 696.4 0 59 132 59 696.4 0 59 133 85 696.4 0 85 134 83 696.4 0 83 135 84 696.4 0 84 136 141 696.4 0 141 137 330 696.4 0 330 138 240 696.4 0 240 139 448 696.4 0 448 140 198 696.4 0 198 141 145 696.4 0 145 142 119 696.4 0 119 143 42 696.4 0 42 144 72 696.4 0 72 145 87 696.4 0 87 146 96 696.4 0 96 147 108 696.4 0 108 148 115 696.4 0 115 149 75 696.4 0 75 150 92 696.4 0 92 151 78 696.4 0 78 152 82 696.4 0 82 153 101 696.4 0 101 154 89 696.4 0 89 155 160 696.4 0 160 156 96 696.4 0 96 157 192 696.4 0 192 158 229 696.4 0 229 159 256 696.4 0 256 160 1448 696.4 0 1448 161 1211 696.4 0 1211 162 1538 696.4 0 1538 163 510 696.4 0 510 164 148 696.4 0 148 165 93 696.4 0 93 166 81 696.4 0 81 167 65 696.4 0 65 168 130 696.4 0 130 169 96 696.4 0 96 170 100 696.4 0 100 171 60 696.4 0 60 172 64 696.4 0 64 173 105 696.4 0 105 174 100 696.4 0 100 175 130 696.4 0 130 176 144 696.4 0 144 177 117 696.4 0 117 178 94 696.4 0 94 179 129 696.4 0 129 180 513 696.4 0 513 181 774 696.4 0 774 182 924 696.4 0 924 183 567 696.4 0 567 184 228 696.4 0 228 185 189 696.4 0 189 186 183 696.4 0 183 187 1415 696.4 0 1415 188 2349 696.4 0 2349 189 1110 696.4 0 1110 190 119 696.4 0 119 191 66 696.4 0 66 192 75 696.4 0 75 193 72 696.4 0 72 194 78 696.4 0 78 195 65 696.4 0 65 196 92 696.4 0 92 197 91 696.4 0 91 198 101 696.4 0 101 199 106 696.4 0 106 200 120 696.4 0 120 201 98 696.4 0 98 202 190 696.4 0 190 203 168 696.4 0 168 204 225 696.4 0 225 205 200 696.4 0 200 206 212 696.4 0 212 207 208 696.4 0 208 208 127 696.4 0 127 209 91 696.4 0 91 210 671 696.4 0 671 211 660 696.4 0 660 212 1334 696.4 0 1334 213 450 696.4 0 450 214 168 696.4 0 168 215 109 696.4 0 109 216 86 696.4 0 86 217 101 696.4 0 101 218 108 696.4 0 108 219 192 696.4 0 192 220 303 696.4 0 303 221 155 696.4 0 155 222 89 696.4 0 89 223 83 696.4 0 83 224 92 696.4 0 92 225 126 696.4 0 126 226 128 696.4 0 128 227 174 696.4 0 174 228 135 696.4 0 135 229 221 696.4 0 221 230 1273 696.4 0 1273 231 796 696.4 0 796 232 1169 696.4 0 1169 233 126 696.4 0 126 234 58 696.4 0 58 235 58 696.4 0 58 236 65 696.4 0 65 237 50 696.4 0 50 238 75 696.4 0 75 239 92 696.4 0 92 240 126 696.4 0 126 241 94 696.4 0 94 242 76 696.4 0 76 243 64 696.4 0 64 244 74 696.4 0 74 245 88 696.4 0 88 246 60 696.4 0 60 247 55 696.4 0 55 248 60 696.4 0 60 249 44 696.4 0 44 250 47 696.4 0 47 251 54 696.4 0 54 252 85 696.4 0 85 253 105 696.4 0 105 254 96 696.4 0 96 255 90 696.4 0 90 256 103 696.4 0 103 257 116 696.4 0 116 258 164 696.4 0 164 259 136 696.4 0 136 260 213 696.4 0 213 261 140 696.4 0 140 262 127 696.4 0 127 263 97 696.4 0 97 264 95 696.4 0 95 265 60 696.4 0 60 266 91 696.4 0 91 267 95 696.4 0 95 268 123 696.4 0 123 269 135 696.4 0 135 270 79 696.4 0 79 271 88 696.4 0 88 272 129 696.4 0 129 273 200 696.4 0 200 274 208 696.4 0 208 275 523 696.4 0 523 276 583 696.4 0 583 277 345 696.4 0 345 278 209 696.4 0 209 279 192 696.4 0 192 280 333 696.4 0 333 281 177 696.4 0 177 282 114 696.4 0 114 283 237 696.4 0 237 284 335 696.4 0 335 285 216 696.4 0 216 286 90 696.4 0 90 287 125 696.4 0 125 288 158 696.4 0 158 289 349 696.4 0 349 290 230 696.4 0 230 291 144 696.4 0 144 292 81 696.4 0 81 293 101 696.4 0 101 294 134 696.4 0 134 295 105 696.4 0 105 296 91 696.4 0 91 297 148 696.4 0 148 298 143 696.4 0 143 299 140 696.4 0 140 300 398 696.4 0 398 301 798 696.4 0 798 Finished in 2.86 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 59,915 Reads with adapters: 15,951 (26.6%) Reads that were too short: 39 (0.1%) Reads written (passing filters): 15,912 (26.6%) Total basepairs processed: 13,837,540 bp Total written (filtered): 4,296,666 bp (31.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 15951 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 12 0.0 3 0 0 8 2 2 19 180 0.0 3 0 150 16 8 6 20 15159 0.0 4 14351 678 99 21 10 21 52 0.0 4 12 38 2 22 9 0.0 4 1 0 5 3 23 46 0.0 4 0 0 1 34 11 27 1 0.0 4 1 29 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 52 2 0.0 4 0 0 0 0 2 55 1 0.0 4 0 0 1 59 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 109 2 0.0 4 0 0 0 0 2 111 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 4 0.0 4 0 0 0 0 4 127 439 0.0 4 0 0 0 0 439 128 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 133 2 0.0 4 0 0 0 1 1 139 1 0.0 4 0 0 0 1 141 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 234 2 0.0 4 0 0 0 1 1 256 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.58 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 15,912 Reads with adapters: 3,060 (19.2%) Reads written (passing filters): 15,912 (100.0%) Total basepairs processed: 4,296,666 bp Total written (filtered): 4,249,464 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3060 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.5% C: 8.4% G: 85.5% T: 3.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 45 248.6 0 45 4 8 62.2 0 8 6 49 3.9 0 49 8 1 0.2 0 1 9 22 0.1 0 1 21 10 363 0.0 1 34 329 11 2465 0.0 1 112 2353 12 30 0.0 1 2 28 13 3 0.0 1 0 3 14 3 0.0 1 0 3 15 1 0.0 1 0 1 73 1 0.0 1 1 151 3 0.0 1 3 209 8 0.0 1 7 1 210 2 0.0 1 2 224 51 0.0 1 47 4 225 1 0.0 1 1 265 3 0.0 1 2 1 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.