This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.52 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 86,926 (3.0%) Reads that were too short: 19,392 (0.7%) Reads written (passing filters): 67,534 (2.4%) Total basepairs processed: 858,556,748 bp Total written (filtered): 15,945,900 bp (1.9%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 86926 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2148 696.4 0 2148 7 18136 696.4 0 18136 8 2260 696.4 0 2260 9 15741 696.4 0 15741 10 16 696.4 0 16 11 13 696.4 0 13 12 27 696.4 0 27 13 19 696.4 0 19 14 24 696.4 0 24 15 10 696.4 0 10 16 24 696.4 0 24 17 21 696.4 0 21 18 20 696.4 0 20 19 23 696.4 0 23 20 17 696.4 0 17 21 20 696.4 0 20 22 11 696.4 0 11 23 19 696.4 0 19 24 8 696.4 0 8 25 12 696.4 0 12 26 12 696.4 0 12 27 20 696.4 0 20 28 31 696.4 0 31 29 25 696.4 0 25 30 50 696.4 0 50 31 100 696.4 0 100 32 853 696.4 0 853 33 435 696.4 0 435 34 41 696.4 0 41 35 27 696.4 0 27 36 21 696.4 0 21 37 24 696.4 0 24 38 97 696.4 0 97 39 52 696.4 0 52 40 48 696.4 0 48 41 17 696.4 0 17 42 16 696.4 0 16 43 28 696.4 0 28 44 16 696.4 0 16 45 19 696.4 0 19 46 20 696.4 0 20 47 16 696.4 0 16 48 12 696.4 0 12 49 12 696.4 0 12 50 23 696.4 0 23 51 24 696.4 0 24 52 27 696.4 0 27 53 239 696.4 0 239 54 193 696.4 0 193 55 78 696.4 0 78 56 24 696.4 0 24 57 27 696.4 0 27 58 27 696.4 0 27 59 23 696.4 0 23 60 29 696.4 0 29 61 8 696.4 0 8 62 21 696.4 0 21 63 12 696.4 0 12 64 21 696.4 0 21 65 21 696.4 0 21 66 22 696.4 0 22 67 20 696.4 0 20 68 28 696.4 0 28 69 116 696.4 0 116 70 49 696.4 0 49 71 137 696.4 0 137 72 26 696.4 0 26 73 23 696.4 0 23 74 13 696.4 0 13 75 23 696.4 0 23 76 11 696.4 0 11 77 14 696.4 0 14 78 24 696.4 0 24 79 23 696.4 0 23 80 18 696.4 0 18 81 35 696.4 0 35 82 32 696.4 0 32 83 24 696.4 0 24 84 19 696.4 0 19 85 26 696.4 0 26 86 36 696.4 0 36 87 52 696.4 0 52 88 36 696.4 0 36 89 53 696.4 0 53 90 47 696.4 0 47 91 69 696.4 0 69 92 33 696.4 0 33 93 76 696.4 0 76 94 53 696.4 0 53 95 75 696.4 0 75 96 33 696.4 0 33 97 20 696.4 0 20 98 19 696.4 0 19 99 19 696.4 0 19 100 31 696.4 0 31 101 42 696.4 0 42 102 52 696.4 0 52 103 95 696.4 0 95 104 113 696.4 0 113 105 99 696.4 0 99 106 56 696.4 0 56 107 60 696.4 0 60 108 53 696.4 0 53 109 52 696.4 0 52 110 36 696.4 0 36 111 80 696.4 0 80 112 81 696.4 0 81 113 141 696.4 0 141 114 47 696.4 0 47 115 61 696.4 0 61 116 46 696.4 0 46 117 42 696.4 0 42 118 27 696.4 0 27 119 36 696.4 0 36 120 54 696.4 0 54 121 36 696.4 0 36 122 63 696.4 0 63 123 94 696.4 0 94 124 130 696.4 0 130 125 1073 696.4 0 1073 126 1546 696.4 0 1546 127 765 696.4 0 765 128 57 696.4 0 57 129 48 696.4 0 48 130 58 696.4 0 58 131 45 696.4 0 45 132 49 696.4 0 49 133 99 696.4 0 99 134 70 696.4 0 70 135 58 696.4 0 58 136 43 696.4 0 43 137 43 696.4 0 43 138 40 696.4 0 40 139 64 696.4 0 64 140 85 696.4 0 85 141 66 696.4 0 66 142 51 696.4 0 51 143 69 696.4 0 69 144 112 696.4 0 112 145 333 696.4 0 333 146 272 696.4 0 272 147 181 696.4 0 181 148 68 696.4 0 68 149 94 696.4 0 94 150 216 696.4 0 216 151 216 696.4 0 216 152 209 696.4 0 209 153 109 696.4 0 109 154 147 696.4 0 147 155 166 696.4 0 166 156 252 696.4 0 252 157 484 696.4 0 484 158 354 696.4 0 354 159 303 696.4 0 303 160 3258 696.4 0 3258 161 3769 696.4 0 3769 162 2485 696.4 0 2485 163 142 696.4 0 142 164 138 696.4 0 138 165 87 696.4 0 87 166 123 696.4 0 123 167 74 696.4 0 74 168 83 696.4 0 83 169 245 696.4 0 245 170 465 696.4 0 465 171 360 696.4 0 360 172 60 696.4 0 60 173 83 696.4 0 83 174 71 696.4 0 71 175 161 696.4 0 161 176 131 696.4 0 131 177 135 696.4 0 135 178 79 696.4 0 79 179 150 696.4 0 150 180 466 696.4 0 466 181 578 696.4 0 578 182 601 696.4 0 601 183 269 696.4 0 269 184 78 696.4 0 78 185 89 696.4 0 89 186 147 696.4 0 147 187 101 696.4 0 101 188 91 696.4 0 91 189 58 696.4 0 58 190 78 696.4 0 78 191 76 696.4 0 76 192 61 696.4 0 61 193 53 696.4 0 53 194 46 696.4 0 46 195 59 696.4 0 59 196 54 696.4 0 54 197 75 696.4 0 75 198 87 696.4 0 87 199 96 696.4 0 96 200 70 696.4 0 70 201 68 696.4 0 68 202 46 696.4 0 46 203 72 696.4 0 72 204 63 696.4 0 63 205 46 696.4 0 46 206 48 696.4 0 48 207 40 696.4 0 40 208 68 696.4 0 68 209 48 696.4 0 48 210 75 696.4 0 75 211 98 696.4 0 98 212 539 696.4 0 539 213 588 696.4 0 588 214 620 696.4 0 620 215 79 696.4 0 79 216 84 696.4 0 84 217 83 696.4 0 83 218 83 696.4 0 83 219 100 696.4 0 100 220 95 696.4 0 95 221 71 696.4 0 71 222 81 696.4 0 81 223 74 696.4 0 74 224 107 696.4 0 107 225 135 696.4 0 135 226 161 696.4 0 161 227 98 696.4 0 98 228 78 696.4 0 78 229 104 696.4 0 104 230 76 696.4 0 76 231 93 696.4 0 93 232 50 696.4 0 50 233 69 696.4 0 69 234 57 696.4 0 57 235 93 696.4 0 93 236 91 696.4 0 91 237 123 696.4 0 123 238 133 696.4 0 133 239 186 696.4 0 186 240 131 696.4 0 131 241 142 696.4 0 142 242 91 696.4 0 91 243 134 696.4 0 134 244 114 696.4 0 114 245 153 696.4 0 153 246 139 696.4 0 139 247 202 696.4 0 202 248 165 696.4 0 165 249 120 696.4 0 120 250 99 696.4 0 99 251 81 696.4 0 81 252 88 696.4 0 88 253 94 696.4 0 94 254 89 696.4 0 89 255 141 696.4 0 141 256 323 696.4 0 323 257 359 696.4 0 359 258 308 696.4 0 308 259 317 696.4 0 317 260 165 696.4 0 165 261 93 696.4 0 93 262 127 696.4 0 127 263 108 696.4 0 108 264 114 696.4 0 114 265 110 696.4 0 110 266 277 696.4 0 277 267 287 696.4 0 287 268 394 696.4 0 394 269 172 696.4 0 172 270 141 696.4 0 141 271 213 696.4 0 213 272 1037 696.4 0 1037 273 1327 696.4 0 1327 274 1176 696.4 0 1176 275 1652 696.4 0 1652 276 151 696.4 0 151 277 161 696.4 0 161 278 189 696.4 0 189 279 142 696.4 0 142 280 306 696.4 0 306 281 178 696.4 0 178 282 158 696.4 0 158 283 119 696.4 0 119 284 155 696.4 0 155 285 141 696.4 0 141 286 180 696.4 0 180 287 285 696.4 0 285 288 240 696.4 0 240 289 219 696.4 0 219 290 92 696.4 0 92 291 89 696.4 0 89 292 105 696.4 0 105 293 184 696.4 0 184 294 106 696.4 0 106 295 100 696.4 0 100 296 73 696.4 0 73 297 81 696.4 0 81 298 92 696.4 0 92 299 95 696.4 0 95 300 128 696.4 0 128 301 185 696.4 0 185 Finished in 2.96 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 67,534 Reads with adapters: 18,270 (27.1%) Reads that were too short: 94 (0.1%) Reads written (passing filters): 18,176 (26.9%) Total basepairs processed: 15,945,900 bp Total written (filtered): 4,996,336 bp (31.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 18270 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 84 0.0 3 0 82 2 17 15981 0.0 3 15624 348 8 1 18 2031 0.0 3 1956 72 2 1 19 14 0.0 3 1 2 8 3 20 40 0.0 3 0 0 3 37 21 5 0.0 3 1 0 0 4 30 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 44 1 0.0 3 1 45 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 59 2 0.0 3 2 60 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 1 74 2 0.0 3 0 0 0 2 75 3 0.0 3 0 0 1 2 80 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 95 1 0.0 3 1 98 1 0.0 3 0 1 99 2 0.0 3 1 1 101 2 0.0 3 1 0 0 1 105 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 129 3 0.0 3 0 0 0 3 163 4 0.0 3 0 0 1 3 165 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 1 1 217 59 0.0 3 0 0 2 57 280 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.64 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 18,176 Reads with adapters: 679 (3.7%) Reads written (passing filters): 18,176 (100.0%) Total basepairs processed: 4,996,336 bp Total written (filtered): 4,994,255 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 679 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 79.8% C: 6.3% G: 13.1% T: 0.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 644 284.0 0 644 4 30 71.0 0 30 5 1 17.8 0 1 6 4 4.4 0 4