This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGATCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.95 s (27 us/read; 2.20 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 428,286 (15.0%) Reads that were too short: 9,289 (0.3%) Reads written (passing filters): 418,997 (14.7%) Total basepairs processed: 858,556,748 bp Total written (filtered): 107,275,640 bp (12.5%) === Adapter 1 === Sequence: TGATCC; Type: regular 5'; Length: 6; Trimmed: 428286 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4716 696.4 0 4716 7 28432 696.4 0 28432 8 27125 696.4 0 27125 9 203 696.4 0 203 10 24 696.4 0 24 11 43 696.4 0 43 12 233 696.4 0 233 13 19 696.4 0 19 14 19 696.4 0 19 15 33 696.4 0 33 16 40 696.4 0 40 17 45 696.4 0 45 18 33 696.4 0 33 19 61 696.4 0 61 20 60 696.4 0 60 21 116 696.4 0 116 22 412 696.4 0 412 23 123 696.4 0 123 24 270 696.4 0 270 25 187 696.4 0 187 26 93 696.4 0 93 27 208 696.4 0 208 28 109 696.4 0 109 29 245 696.4 0 245 30 277 696.4 0 277 31 205 696.4 0 205 32 194 696.4 0 194 33 325 696.4 0 325 34 663 696.4 0 663 35 845 696.4 0 845 36 1393 696.4 0 1393 37 2715 696.4 0 2715 38 5513 696.4 0 5513 39 72505 696.4 0 72505 40 51986 696.4 0 51986 41 39206 696.4 0 39206 42 325 696.4 0 325 43 187 696.4 0 187 44 288 696.4 0 288 45 586 696.4 0 586 46 1354 696.4 0 1354 47 356 696.4 0 356 48 448 696.4 0 448 49 877 696.4 0 877 50 320 696.4 0 320 51 323 696.4 0 323 52 659 696.4 0 659 53 806 696.4 0 806 54 962 696.4 0 962 55 619 696.4 0 619 56 1153 696.4 0 1153 57 1681 696.4 0 1681 58 2901 696.4 0 2901 59 52801 696.4 0 52801 60 53444 696.4 0 53444 61 50927 696.4 0 50927 62 1721 696.4 0 1721 63 14 696.4 0 14 64 13 696.4 0 13 65 4 696.4 0 4 66 8 696.4 0 8 67 3 696.4 0 3 68 9 696.4 0 9 69 5 696.4 0 5 70 7 696.4 0 7 71 9 696.4 0 9 72 17 696.4 0 17 73 29 696.4 0 29 74 24 696.4 0 24 75 38 696.4 0 38 76 7 696.4 0 7 77 6 696.4 0 6 78 8 696.4 0 8 79 14 696.4 0 14 80 16 696.4 0 16 81 36 696.4 0 36 82 31 696.4 0 31 83 35 696.4 0 35 84 11 696.4 0 11 85 2 696.4 0 2 86 54 696.4 0 54 87 22 696.4 0 22 88 16 696.4 0 16 89 9 696.4 0 9 90 24 696.4 0 24 91 5 696.4 0 5 92 10 696.4 0 10 93 9 696.4 0 9 94 14 696.4 0 14 95 9 696.4 0 9 96 4 696.4 0 4 97 20 696.4 0 20 98 10 696.4 0 10 99 4 696.4 0 4 100 39 696.4 0 39 101 497 696.4 0 497 102 963 696.4 0 963 103 430 696.4 0 430 104 8 696.4 0 8 105 11 696.4 0 11 106 9 696.4 0 9 107 5 696.4 0 5 108 4 696.4 0 4 109 11 696.4 0 11 110 37 696.4 0 37 111 164 696.4 0 164 112 144 696.4 0 144 113 184 696.4 0 184 114 11 696.4 0 11 115 3 696.4 0 3 116 12 696.4 0 12 117 15 696.4 0 15 118 16 696.4 0 16 119 9 696.4 0 9 120 13 696.4 0 13 121 9 696.4 0 9 122 12 696.4 0 12 123 12 696.4 0 12 124 14 696.4 0 14 125 17 696.4 0 17 126 8 696.4 0 8 127 6 696.4 0 6 128 13 696.4 0 13 129 25 696.4 0 25 130 21 696.4 0 21 131 29 696.4 0 29 132 48 696.4 0 48 133 14 696.4 0 14 134 16 696.4 0 16 135 11 696.4 0 11 136 22 696.4 0 22 137 28 696.4 0 28 138 17 696.4 0 17 139 12 696.4 0 12 140 12 696.4 0 12 141 29 696.4 0 29 142 23 696.4 0 23 143 42 696.4 0 42 144 15 696.4 0 15 145 17 696.4 0 17 146 17 696.4 0 17 147 15 696.4 0 15 148 21 696.4 0 21 149 34 696.4 0 34 150 19 696.4 0 19 151 20 696.4 0 20 152 18 696.4 0 18 153 13 696.4 0 13 154 7 696.4 0 7 155 13 696.4 0 13 156 23 696.4 0 23 157 24 696.4 0 24 158 36 696.4 0 36 159 16 696.4 0 16 160 12 696.4 0 12 161 35 696.4 0 35 162 89 696.4 0 89 163 46 696.4 0 46 164 46 696.4 0 46 165 13 696.4 0 13 166 15 696.4 0 15 167 31 696.4 0 31 168 36 696.4 0 36 169 62 696.4 0 62 170 123 696.4 0 123 171 68 696.4 0 68 172 31 696.4 0 31 173 13 696.4 0 13 174 15 696.4 0 15 175 15 696.4 0 15 176 21 696.4 0 21 177 23 696.4 0 23 178 36 696.4 0 36 179 28 696.4 0 28 180 21 696.4 0 21 181 29 696.4 0 29 182 34 696.4 0 34 183 82 696.4 0 82 184 90 696.4 0 90 185 164 696.4 0 164 186 94 696.4 0 94 187 134 696.4 0 134 188 30 696.4 0 30 189 37 696.4 0 37 190 30 696.4 0 30 191 16 696.4 0 16 192 23 696.4 0 23 193 26 696.4 0 26 194 40 696.4 0 40 195 50 696.4 0 50 196 64 696.4 0 64 197 171 696.4 0 171 198 564 696.4 0 564 199 424 696.4 0 424 200 642 696.4 0 642 201 171 696.4 0 171 202 65 696.4 0 65 203 239 696.4 0 239 204 194 696.4 0 194 205 369 696.4 0 369 206 89 696.4 0 89 207 31 696.4 0 31 208 25 696.4 0 25 209 20 696.4 0 20 210 22 696.4 0 22 211 51 696.4 0 51 212 21 696.4 0 21 213 46 696.4 0 46 214 26 696.4 0 26 215 21 696.4 0 21 216 28 696.4 0 28 217 32 696.4 0 32 218 61 696.4 0 61 219 55 696.4 0 55 220 99 696.4 0 99 221 59 696.4 0 59 222 70 696.4 0 70 223 623 696.4 0 623 224 448 696.4 0 448 225 719 696.4 0 719 226 675 696.4 0 675 227 696 696.4 0 696 228 649 696.4 0 649 229 201 696.4 0 201 230 61 696.4 0 61 231 9 696.4 0 9 232 15 696.4 0 15 233 10 696.4 0 10 234 17 696.4 0 17 235 27 696.4 0 27 236 21 696.4 0 21 237 18 696.4 0 18 238 23 696.4 0 23 239 32 696.4 0 32 240 18 696.4 0 18 241 22 696.4 0 22 242 31 696.4 0 31 243 34 696.4 0 34 244 23 696.4 0 23 245 27 696.4 0 27 246 22 696.4 0 22 247 26 696.4 0 26 248 32 696.4 0 32 249 26 696.4 0 26 250 28 696.4 0 28 251 23 696.4 0 23 252 18 696.4 0 18 253 19 696.4 0 19 254 28 696.4 0 28 255 68 696.4 0 68 256 106 696.4 0 106 257 160 696.4 0 160 258 133 696.4 0 133 259 131 696.4 0 131 260 56 696.4 0 56 261 94 696.4 0 94 262 37 696.4 0 37 263 39 696.4 0 39 264 37 696.4 0 37 265 46 696.4 0 46 266 26 696.4 0 26 267 22 696.4 0 22 268 19 696.4 0 19 269 16 696.4 0 16 270 28 696.4 0 28 271 53 696.4 0 53 272 80 696.4 0 80 273 114 696.4 0 114 274 127 696.4 0 127 275 71 696.4 0 71 276 71 696.4 0 71 277 30 696.4 0 30 278 24 696.4 0 24 279 43 696.4 0 43 280 34 696.4 0 34 281 51 696.4 0 51 282 29 696.4 0 29 283 31 696.4 0 31 284 41 696.4 0 41 285 66 696.4 0 66 286 67 696.4 0 67 287 76 696.4 0 76 288 50 696.4 0 50 289 48 696.4 0 48 290 65 696.4 0 65 291 90 696.4 0 90 292 132 696.4 0 132 293 155 696.4 0 155 294 69 696.4 0 69 295 71 696.4 0 71 296 48 696.4 0 48 297 44 696.4 0 44 298 66 696.4 0 66 299 65 696.4 0 65 300 47 696.4 0 47 301 69 696.4 0 69 Finished in 19.75 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 418,997 Reads with adapters: 41,928 (10.0%) Reads that were too short: 288 (0.1%) Reads written (passing filters): 41,640 (9.9%) Total basepairs processed: 107,275,640 bp Total written (filtered): 9,941,406 bp (9.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 41928 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 1 1 1 19 209 0.0 3 0 160 23 18 8 20 27409 0.0 4 25930 1208 198 60 13 21 80 0.0 4 10 62 6 2 22 17 0.0 4 3 5 8 1 23 486 0.0 4 374 17 4 69 22 24 5 0.0 4 0 2 0 1 2 25 1 0.0 4 1 26 3 0.0 4 0 0 0 3 40 1 0.0 4 0 0 0 0 1 45 1 0.0 4 1 68 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 73 20 0.0 4 0 0 0 0 20 74 29 0.0 4 0 0 0 0 29 75 13097 0.0 4 0 0 0 0 13097 78 18 0.0 4 0 0 0 0 18 79 2 0.0 4 0 0 0 0 2 83 5 0.0 4 1 1 0 0 3 85 1 0.0 4 0 0 0 0 1 87 1 0.0 4 1 88 4 0.0 4 0 1 0 0 3 90 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 1 97 1 0.0 4 1 98 1 0.0 4 0 0 0 0 1 101 2 0.0 4 0 0 0 0 2 103 2 0.0 4 0 1 1 114 1 0.0 4 0 0 1 122 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 227 0.0 4 0 0 0 0 227 130 3 0.0 4 0 0 0 0 3 132 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 155 2 0.0 4 0 0 0 0 2 156 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 1 167 1 0.0 4 0 0 0 0 1 169 7 0.0 4 0 0 0 0 7 170 4 0.0 4 0 0 0 0 4 171 5 0.0 4 0 0 0 0 5 173 8 0.0 4 0 0 0 0 8 174 2 0.0 4 0 0 0 0 2 175 1 0.0 4 0 0 0 0 1 176 87 0.0 4 0 0 0 3 84 177 23 0.0 4 0 0 0 2 21 178 6 0.0 4 0 0 0 0 6 180 5 0.0 4 0 0 0 0 5 181 1 0.0 4 0 0 0 0 1 182 7 0.0 4 0 0 0 0 7 183 7 0.0 4 0 0 0 1 6 184 38 0.0 4 0 0 0 0 38 186 3 0.0 4 0 0 0 0 3 190 1 0.0 4 0 1 191 1 0.0 4 0 0 1 202 3 0.0 4 0 0 0 0 3 204 3 0.0 4 0 0 0 0 3 205 4 0.0 4 0 0 0 0 4 206 2 0.0 4 0 0 0 0 2 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 227 2 0.0 4 0 0 0 0 2 228 1 0.0 4 0 0 0 0 1 230 13 0.0 4 0 0 0 1 12 231 5 0.0 4 0 0 0 0 5 232 2 0.0 4 0 0 0 0 2 233 3 0.0 4 0 0 0 0 3 234 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 236 8 0.0 4 0 0 0 0 8 237 3 0.0 4 0 0 0 0 3 238 1 0.0 4 0 0 0 0 1 239 4 0.0 4 0 0 0 0 4 240 2 0.0 4 0 0 0 0 2 241 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 2 0.0 4 0 0 0 0 2 266 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.36 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 41,640 Reads with adapters: 5,573 (13.4%) Reads written (passing filters): 41,640 (100.0%) Total basepairs processed: 9,941,406 bp Total written (filtered): 9,797,892 bp (98.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5573 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 13.3% C: 9.1% G: 75.7% T: 1.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 74 650.6 0 74 4 94 162.7 0 94 5 4 40.7 0 4 6 1 10.2 0 1 9 52 0.2 0 6 46 10 1217 0.0 1 114 1103 11 3581 0.0 1 159 3422 12 29 0.0 1 1 28 13 3 0.0 1 0 3 14 5 0.0 1 2 3 18 1 0.0 1 0 1 19 143 0.0 1 92 51 20 9 0.0 1 6 3 21 2 0.0 1 2 22 1 0.0 1 1 23 14 0.0 1 11 3 24 1 0.0 1 1 49 2 0.0 1 1 1 151 1 0.0 1 0 1 190 2 0.0 1 0 2 209 17 0.0 1 13 4 256 1 0.0 1 1 259 305 0.0 1 284 21 260 8 0.0 1 7 1 261 6 0.0 1 6