This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGATCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.80 s (28 us/read; 2.17 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 493,555 (17.3%) Reads that were too short: 5,539 (0.2%) Reads written (passing filters): 488,016 (17.1%) Total basepairs processed: 858,556,748 bp Total written (filtered): 125,842,474 bp (14.7%) === Adapter 1 === Sequence: TGATCC; Type: regular 5'; Length: 6; Trimmed: 493555 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2197 696.4 0 2197 7 26069 696.4 0 26069 8 34902 696.4 0 34902 9 146 696.4 0 146 10 27 696.4 0 27 11 54 696.4 0 54 12 349 696.4 0 349 13 25 696.4 0 25 14 22 696.4 0 22 15 38 696.4 0 38 16 48 696.4 0 48 17 50 696.4 0 50 18 28 696.4 0 28 19 80 696.4 0 80 20 67 696.4 0 67 21 162 696.4 0 162 22 580 696.4 0 580 23 152 696.4 0 152 24 348 696.4 0 348 25 249 696.4 0 249 26 134 696.4 0 134 27 279 696.4 0 279 28 129 696.4 0 129 29 208 696.4 0 208 30 152 696.4 0 152 31 200 696.4 0 200 32 242 696.4 0 242 33 375 696.4 0 375 34 824 696.4 0 824 35 1056 696.4 0 1056 36 1774 696.4 0 1774 37 3572 696.4 0 3572 38 5477 696.4 0 5477 39 104281 696.4 0 104281 40 75169 696.4 0 75169 41 57327 696.4 0 57327 42 365 696.4 0 365 43 190 696.4 0 190 44 311 696.4 0 311 45 573 696.4 0 573 46 1295 696.4 0 1295 47 331 696.4 0 331 48 412 696.4 0 412 49 838 696.4 0 838 50 274 696.4 0 274 51 303 696.4 0 303 52 621 696.4 0 621 53 735 696.4 0 735 54 818 696.4 0 818 55 551 696.4 0 551 56 1017 696.4 0 1017 57 1333 696.4 0 1333 58 2318 696.4 0 2318 59 49920 696.4 0 49920 60 51637 696.4 0 51637 61 50961 696.4 0 50961 62 1697 696.4 0 1697 63 14 696.4 0 14 64 7 696.4 0 7 65 3 696.4 0 3 66 4 696.4 0 4 67 4 696.4 0 4 68 2 696.4 0 2 69 3 696.4 0 3 70 2 696.4 0 2 71 6 696.4 0 6 72 11 696.4 0 11 73 11 696.4 0 11 74 16 696.4 0 16 75 40 696.4 0 40 76 6 696.4 0 6 77 2 696.4 0 2 78 3 696.4 0 3 79 7 696.4 0 7 80 10 696.4 0 10 81 26 696.4 0 26 82 14 696.4 0 14 83 26 696.4 0 26 84 9 696.4 0 9 85 8 696.4 0 8 86 2 696.4 0 2 87 8 696.4 0 8 88 4 696.4 0 4 89 5 696.4 0 5 90 16 696.4 0 16 91 15 696.4 0 15 92 18 696.4 0 18 93 6 696.4 0 6 94 5 696.4 0 5 95 8 696.4 0 8 96 10 696.4 0 10 97 11 696.4 0 11 98 19 696.4 0 19 99 9 696.4 0 9 100 21 696.4 0 21 101 300 696.4 0 300 102 523 696.4 0 523 103 216 696.4 0 216 104 8 696.4 0 8 105 10 696.4 0 10 106 6 696.4 0 6 107 6 696.4 0 6 108 6 696.4 0 6 109 14 696.4 0 14 110 21 696.4 0 21 111 133 696.4 0 133 112 151 696.4 0 151 113 223 696.4 0 223 114 7 696.4 0 7 115 7 696.4 0 7 116 7 696.4 0 7 117 12 696.4 0 12 118 11 696.4 0 11 119 12 696.4 0 12 120 9 696.4 0 9 121 7 696.4 0 7 122 7 696.4 0 7 123 8 696.4 0 8 124 11 696.4 0 11 125 14 696.4 0 14 126 5 696.4 0 5 127 6 696.4 0 6 128 4 696.4 0 4 129 12 696.4 0 12 130 16 696.4 0 16 131 28 696.4 0 28 132 29 696.4 0 29 133 13 696.4 0 13 134 10 696.4 0 10 135 5 696.4 0 5 136 23 696.4 0 23 137 15 696.4 0 15 138 5 696.4 0 5 139 11 696.4 0 11 140 4 696.4 0 4 141 13 696.4 0 13 142 13 696.4 0 13 143 23 696.4 0 23 144 3 696.4 0 3 145 10 696.4 0 10 146 18 696.4 0 18 147 4 696.4 0 4 148 23 696.4 0 23 149 27 696.4 0 27 150 21 696.4 0 21 151 21 696.4 0 21 152 18 696.4 0 18 153 8 696.4 0 8 154 8 696.4 0 8 155 7 696.4 0 7 156 14 696.4 0 14 157 19 696.4 0 19 158 24 696.4 0 24 159 10 696.4 0 10 160 10 696.4 0 10 161 20 696.4 0 20 162 35 696.4 0 35 163 27 696.4 0 27 164 16 696.4 0 16 165 9 696.4 0 9 166 9 696.4 0 9 167 24 696.4 0 24 168 29 696.4 0 29 169 39 696.4 0 39 170 152 696.4 0 152 171 60 696.4 0 60 172 17 696.4 0 17 173 10 696.4 0 10 174 3 696.4 0 3 175 8 696.4 0 8 176 13 696.4 0 13 177 13 696.4 0 13 178 24 696.4 0 24 179 13 696.4 0 13 180 20 696.4 0 20 181 6 696.4 0 6 182 10 696.4 0 10 183 27 696.4 0 27 184 52 696.4 0 52 185 105 696.4 0 105 186 75 696.4 0 75 187 135 696.4 0 135 188 20 696.4 0 20 189 32 696.4 0 32 190 13 696.4 0 13 191 8 696.4 0 8 192 6 696.4 0 6 193 17 696.4 0 17 194 23 696.4 0 23 195 23 696.4 0 23 196 29 696.4 0 29 197 124 696.4 0 124 198 332 696.4 0 332 199 197 696.4 0 197 200 288 696.4 0 288 201 61 696.4 0 61 202 33 696.4 0 33 203 50 696.4 0 50 204 47 696.4 0 47 205 75 696.4 0 75 206 28 696.4 0 28 207 12 696.4 0 12 208 8 696.4 0 8 209 22 696.4 0 22 210 13 696.4 0 13 211 32 696.4 0 32 212 12 696.4 0 12 213 20 696.4 0 20 214 24 696.4 0 24 215 13 696.4 0 13 216 14 696.4 0 14 217 17 696.4 0 17 218 33 696.4 0 33 219 29 696.4 0 29 220 44 696.4 0 44 221 29 696.4 0 29 222 30 696.4 0 30 223 219 696.4 0 219 224 168 696.4 0 168 225 284 696.4 0 284 226 636 696.4 0 636 227 775 696.4 0 775 228 871 696.4 0 871 229 237 696.4 0 237 230 38 696.4 0 38 231 7 696.4 0 7 232 4 696.4 0 4 233 9 696.4 0 9 234 8 696.4 0 8 235 12 696.4 0 12 236 14 696.4 0 14 237 12 696.4 0 12 238 16 696.4 0 16 239 14 696.4 0 14 240 15 696.4 0 15 241 12 696.4 0 12 242 11 696.4 0 11 243 21 696.4 0 21 244 11 696.4 0 11 245 14 696.4 0 14 246 14 696.4 0 14 247 20 696.4 0 20 248 23 696.4 0 23 249 23 696.4 0 23 250 10 696.4 0 10 251 13 696.4 0 13 252 19 696.4 0 19 253 11 696.4 0 11 254 8 696.4 0 8 255 29 696.4 0 29 256 45 696.4 0 45 257 78 696.4 0 78 258 54 696.4 0 54 259 34 696.4 0 34 260 21 696.4 0 21 261 33 696.4 0 33 262 19 696.4 0 19 263 22 696.4 0 22 264 28 696.4 0 28 265 24 696.4 0 24 266 9 696.4 0 9 267 12 696.4 0 12 268 17 696.4 0 17 269 17 696.4 0 17 270 16 696.4 0 16 271 34 696.4 0 34 272 19 696.4 0 19 273 39 696.4 0 39 274 59 696.4 0 59 275 43 696.4 0 43 276 54 696.4 0 54 277 16 696.4 0 16 278 14 696.4 0 14 279 15 696.4 0 15 280 11 696.4 0 11 281 24 696.4 0 24 282 15 696.4 0 15 283 22 696.4 0 22 284 27 696.4 0 27 285 37 696.4 0 37 286 24 696.4 0 24 287 30 696.4 0 30 288 19 696.4 0 19 289 25 696.4 0 25 290 32 696.4 0 32 291 40 696.4 0 40 292 51 696.4 0 51 293 78 696.4 0 78 294 33 696.4 0 33 295 27 696.4 0 27 296 11 696.4 0 11 297 20 696.4 0 20 298 31 696.4 0 31 299 28 696.4 0 28 300 28 696.4 0 28 301 41 696.4 0 41 Finished in 19.79 s (41 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 488,016 Reads with adapters: 27,390 (5.6%) Reads that were too short: 99 (0.0%) Reads written (passing filters): 27,291 (5.6%) Total basepairs processed: 125,842,474 bp Total written (filtered): 7,500,434 bp (6.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 27390 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 1 0.0 3 0 0 1 16 107 0.0 3 0 105 2 17 26710 0.0 3 26270 421 17 2 18 52 0.0 3 5 45 2 19 3 0.0 3 0 0 0 3 20 51 0.0 3 0 0 0 51 26 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 48 7 0.0 3 6 1 63 1 0.0 3 0 0 0 1 80 2 0.0 3 1 0 0 1 81 4 0.0 3 0 0 0 4 84 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 1 93 2 0.0 3 1 0 0 1 95 1 0.0 3 0 0 0 1 97 1 0.0 3 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 105 1 0.0 3 1 107 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 110 5 0.0 3 0 0 0 5 112 8 0.0 3 0 0 0 8 114 1 0.0 3 0 0 0 1 115 3 0.0 3 0 0 0 3 123 2 0.0 3 0 0 0 2 127 6 0.0 3 0 0 0 6 129 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 131 5 0.0 3 0 0 0 5 132 231 0.0 3 0 0 0 231 133 18 0.0 3 0 0 0 18 134 2 0.0 3 0 0 0 2 135 26 0.0 3 0 0 0 26 136 6 0.0 3 0 0 0 6 137 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 159 2 0.0 3 0 0 0 2 160 18 0.0 3 0 0 0 18 161 1 0.0 3 0 0 0 1 165 1 0.0 3 0 1 167 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 183 5 0.0 3 0 0 0 5 184 2 0.0 3 0 0 0 2 185 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 196 2 0.0 3 1 0 0 1 200 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 232 2 0.0 3 0 0 0 2 233 7 0.0 3 0 0 0 7 234 23 0.0 3 0 0 0 23 235 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 247 8 0.0 3 0 0 0 8 248 3 0.0 3 0 0 0 3 249 10 0.0 3 0 0 0 10 250 2 0.0 3 0 0 0 2 257 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.98 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 27,291 Reads with adapters: 472 (1.7%) Reads written (passing filters): 27,291 (100.0%) Total basepairs processed: 7,500,434 bp Total written (filtered): 7,499,003 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 472 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 86.4% C: 5.9% G: 5.7% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 459 426.4 0 459 4 12 106.6 0 12 6 1 6.7 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.