This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCCTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.02 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 112,427 (3.9%) Reads that were too short: 14,957 (0.5%) Reads written (passing filters): 97,470 (3.4%) Total basepairs processed: 858,556,748 bp Total written (filtered): 23,895,118 bp (2.8%) === Adapter 1 === Sequence: TTCCTT; Type: regular 5'; Length: 6; Trimmed: 112427 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 394 696.4 0 394 7 740 696.4 0 740 8 25957 696.4 0 25957 9 22714 696.4 0 22714 10 45 696.4 0 45 11 80 696.4 0 80 12 77 696.4 0 77 13 143 696.4 0 143 14 32 696.4 0 32 15 25 696.4 0 25 16 20 696.4 0 20 17 49 696.4 0 49 18 64 696.4 0 64 19 27 696.4 0 27 20 36 696.4 0 36 21 29 696.4 0 29 22 50 696.4 0 50 23 35 696.4 0 35 24 30 696.4 0 30 25 67 696.4 0 67 26 70 696.4 0 70 27 43 696.4 0 43 28 35 696.4 0 35 29 30 696.4 0 30 30 66 696.4 0 66 31 415 696.4 0 415 32 572 696.4 0 572 33 210 696.4 0 210 34 38 696.4 0 38 35 51 696.4 0 51 36 25 696.4 0 25 37 56 696.4 0 56 38 63 696.4 0 63 39 60 696.4 0 60 40 43 696.4 0 43 41 57 696.4 0 57 42 76 696.4 0 76 43 59 696.4 0 59 44 37 696.4 0 37 45 35 696.4 0 35 46 58 696.4 0 58 47 55 696.4 0 55 48 111 696.4 0 111 49 62 696.4 0 62 50 54 696.4 0 54 51 132 696.4 0 132 52 67 696.4 0 67 53 132 696.4 0 132 54 44 696.4 0 44 55 68 696.4 0 68 56 100 696.4 0 100 57 92 696.4 0 92 58 43 696.4 0 43 59 31 696.4 0 31 60 54 696.4 0 54 61 41 696.4 0 41 62 46 696.4 0 46 63 31 696.4 0 31 64 45 696.4 0 45 65 50 696.4 0 50 66 111 696.4 0 111 67 72 696.4 0 72 68 44 696.4 0 44 69 52 696.4 0 52 70 83 696.4 0 83 71 46 696.4 0 46 72 61 696.4 0 61 73 47 696.4 0 47 74 57 696.4 0 57 75 63 696.4 0 63 76 109 696.4 0 109 77 75 696.4 0 75 78 43 696.4 0 43 79 34 696.4 0 34 80 34 696.4 0 34 81 72 696.4 0 72 82 107 696.4 0 107 83 98 696.4 0 98 84 85 696.4 0 85 85 192 696.4 0 192 86 1171 696.4 0 1171 87 544 696.4 0 544 88 525 696.4 0 525 89 151 696.4 0 151 90 399 696.4 0 399 91 96 696.4 0 96 92 87 696.4 0 87 93 125 696.4 0 125 94 87 696.4 0 87 95 80 696.4 0 80 96 131 696.4 0 131 97 119 696.4 0 119 98 175 696.4 0 175 99 174 696.4 0 174 100 230 696.4 0 230 101 461 696.4 0 461 102 708 696.4 0 708 103 9013 696.4 0 9013 104 9096 696.4 0 9096 105 8000 696.4 0 8000 106 779 696.4 0 779 107 998 696.4 0 998 108 339 696.4 0 339 109 59 696.4 0 59 110 87 696.4 0 87 111 89 696.4 0 89 112 103 696.4 0 103 113 60 696.4 0 60 114 45 696.4 0 45 115 59 696.4 0 59 116 42 696.4 0 42 117 39 696.4 0 39 118 58 696.4 0 58 119 87 696.4 0 87 120 52 696.4 0 52 121 40 696.4 0 40 122 39 696.4 0 39 123 46 696.4 0 46 124 55 696.4 0 55 125 81 696.4 0 81 126 114 696.4 0 114 127 47 696.4 0 47 128 54 696.4 0 54 129 69 696.4 0 69 130 48 696.4 0 48 131 72 696.4 0 72 132 78 696.4 0 78 133 101 696.4 0 101 134 137 696.4 0 137 135 111 696.4 0 111 136 57 696.4 0 57 137 74 696.4 0 74 138 170 696.4 0 170 139 270 696.4 0 270 140 188 696.4 0 188 141 113 696.4 0 113 142 68 696.4 0 68 143 151 696.4 0 151 144 124 696.4 0 124 145 167 696.4 0 167 146 66 696.4 0 66 147 71 696.4 0 71 148 74 696.4 0 74 149 108 696.4 0 108 150 227 696.4 0 227 151 253 696.4 0 253 152 203 696.4 0 203 153 91 696.4 0 91 154 85 696.4 0 85 155 90 696.4 0 90 156 101 696.4 0 101 157 112 696.4 0 112 158 101 696.4 0 101 159 101 696.4 0 101 160 142 696.4 0 142 161 77 696.4 0 77 162 59 696.4 0 59 163 52 696.4 0 52 164 81 696.4 0 81 165 62 696.4 0 62 166 71 696.4 0 71 167 58 696.4 0 58 168 56 696.4 0 56 169 52 696.4 0 52 170 55 696.4 0 55 171 53 696.4 0 53 172 92 696.4 0 92 173 100 696.4 0 100 174 56 696.4 0 56 175 40 696.4 0 40 176 47 696.4 0 47 177 45 696.4 0 45 178 244 696.4 0 244 179 192 696.4 0 192 180 278 696.4 0 278 181 130 696.4 0 130 182 63 696.4 0 63 183 79 696.4 0 79 184 95 696.4 0 95 185 173 696.4 0 173 186 107 696.4 0 107 187 70 696.4 0 70 188 61 696.4 0 61 189 92 696.4 0 92 190 94 696.4 0 94 191 84 696.4 0 84 192 58 696.4 0 58 193 52 696.4 0 52 194 47 696.4 0 47 195 59 696.4 0 59 196 62 696.4 0 62 197 68 696.4 0 68 198 60 696.4 0 60 199 72 696.4 0 72 200 69 696.4 0 69 201 82 696.4 0 82 202 80 696.4 0 80 203 54 696.4 0 54 204 57 696.4 0 57 205 180 696.4 0 180 206 215 696.4 0 215 207 254 696.4 0 254 208 136 696.4 0 136 209 82 696.4 0 82 210 77 696.4 0 77 211 135 696.4 0 135 212 139 696.4 0 139 213 196 696.4 0 196 214 161 696.4 0 161 215 177 696.4 0 177 216 139 696.4 0 139 217 73 696.4 0 73 218 75 696.4 0 75 219 122 696.4 0 122 220 109 696.4 0 109 221 119 696.4 0 119 222 96 696.4 0 96 223 109 696.4 0 109 224 87 696.4 0 87 225 118 696.4 0 118 226 104 696.4 0 104 227 76 696.4 0 76 228 59 696.4 0 59 229 77 696.4 0 77 230 64 696.4 0 64 231 93 696.4 0 93 232 77 696.4 0 77 233 147 696.4 0 147 234 118 696.4 0 118 235 168 696.4 0 168 236 77 696.4 0 77 237 69 696.4 0 69 238 72 696.4 0 72 239 88 696.4 0 88 240 103 696.4 0 103 241 133 696.4 0 133 242 96 696.4 0 96 243 80 696.4 0 80 244 66 696.4 0 66 245 89 696.4 0 89 246 69 696.4 0 69 247 99 696.4 0 99 248 87 696.4 0 87 249 73 696.4 0 73 250 57 696.4 0 57 251 89 696.4 0 89 252 104 696.4 0 104 253 70 696.4 0 70 254 92 696.4 0 92 255 114 696.4 0 114 256 112 696.4 0 112 257 125 696.4 0 125 258 154 696.4 0 154 259 446 696.4 0 446 260 469 696.4 0 469 261 1514 696.4 0 1514 262 819 696.4 0 819 263 940 696.4 0 940 264 105 696.4 0 105 265 108 696.4 0 108 266 83 696.4 0 83 267 134 696.4 0 134 268 71 696.4 0 71 269 52 696.4 0 52 270 55 696.4 0 55 271 73 696.4 0 73 272 83 696.4 0 83 273 106 696.4 0 106 274 180 696.4 0 180 275 167 696.4 0 167 276 117 696.4 0 117 277 86 696.4 0 86 278 103 696.4 0 103 279 267 696.4 0 267 280 244 696.4 0 244 281 206 696.4 0 206 282 125 696.4 0 125 283 148 696.4 0 148 284 143 696.4 0 143 285 63 696.4 0 63 286 77 696.4 0 77 287 83 696.4 0 83 288 136 696.4 0 136 289 80 696.4 0 80 290 83 696.4 0 83 291 49 696.4 0 49 292 66 696.4 0 66 293 102 696.4 0 102 294 110 696.4 0 110 295 131 696.4 0 131 296 92 696.4 0 92 297 114 696.4 0 114 298 82 696.4 0 82 299 144 696.4 0 144 300 295 696.4 0 295 301 315 696.4 0 315 Finished in 4.72 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 97,470 Reads with adapters: 24,473 (25.1%) Reads that were too short: 27 (0.0%) Reads written (passing filters): 24,446 (25.1%) Total basepairs processed: 23,895,118 bp Total written (filtered): 6,564,422 bp (27.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 24473 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 4 0.0 3 0 0 0 2 2 18 10 0.0 3 0 0 5 4 1 19 135 0.0 3 0 104 12 11 8 20 23259 0.0 4 21806 1149 201 76 27 21 79 0.0 4 12 59 4 2 2 22 6 0.0 4 1 0 4 1 23 139 0.0 4 0 0 1 108 30 24 3 0.0 4 1 0 0 0 2 34 1 0.0 4 1 38 1 0.0 4 1 46 1 0.0 4 1 83 1 0.0 4 0 1 84 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 1 91 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 12 0.0 4 0 0 0 0 12 127 783 0.0 4 0 0 0 0 783 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 1 234 4 0.0 4 0 0 0 0 4 235 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.90 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 24,446 Reads with adapters: 6,371 (26.1%) Reads written (passing filters): 24,446 (100.0%) Total basepairs processed: 6,564,422 bp Total written (filtered): 6,499,060 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6371 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.9% C: 8.4% G: 87.8% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 382.0 0 3 4 8 95.5 0 8 5 1 23.9 0 1 6 1 6.0 0 1 8 3 0.4 0 3 9 2314 0.1 0 275 2039 10 3622 0.0 1 301 3321 11 370 0.0 1 17 353 12 18 0.0 1 0 18 13 9 0.0 1 0 9 14 1 0.0 1 0 1 150 7 0.0 1 6 1 151 1 0.0 1 1 160 3 0.0 1 3 208 5 0.0 1 5 209 1 0.0 1 0 1 223 3 0.0 1 3 224 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.