This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.18 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 280,191 (9.8%) Reads that were too short: 45,508 (1.6%) Reads written (passing filters): 234,683 (8.2%) Total basepairs processed: 858,556,748 bp Total written (filtered): 61,089,897 bp (7.1%) === Adapter 1 === Sequence: GTGTAG; Type: regular 5'; Length: 6; Trimmed: 280191 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1070 696.4 0 1070 7 50311 696.4 0 50311 8 38829 696.4 0 38829 9 94 696.4 0 94 10 91 696.4 0 91 11 60 696.4 0 60 12 23 696.4 0 23 13 36 696.4 0 36 14 21 696.4 0 21 15 25 696.4 0 25 16 532 696.4 0 532 17 31 696.4 0 31 18 53 696.4 0 53 19 93 696.4 0 93 20 51016 696.4 0 51016 21 12526 696.4 0 12526 22 148 696.4 0 148 23 99 696.4 0 99 24 98 696.4 0 98 25 32 696.4 0 32 26 27 696.4 0 27 27 30 696.4 0 30 28 33 696.4 0 33 29 75 696.4 0 75 30 37 696.4 0 37 31 44 696.4 0 44 32 36 696.4 0 36 33 23206 696.4 0 23206 34 734 696.4 0 734 35 48 696.4 0 48 36 38 696.4 0 38 37 35 696.4 0 35 38 43 696.4 0 43 39 46 696.4 0 46 40 39 696.4 0 39 41 47 696.4 0 47 42 31 696.4 0 31 43 52 696.4 0 52 44 51 696.4 0 51 45 42 696.4 0 42 46 47 696.4 0 47 47 46 696.4 0 46 48 84 696.4 0 84 49 62 696.4 0 62 50 65 696.4 0 65 51 37 696.4 0 37 52 50 696.4 0 50 53 54 696.4 0 54 54 69 696.4 0 69 55 117 696.4 0 117 56 83 696.4 0 83 57 64 696.4 0 64 58 63 696.4 0 63 59 53 696.4 0 53 60 173 696.4 0 173 61 102 696.4 0 102 62 94 696.4 0 94 63 96 696.4 0 96 64 81 696.4 0 81 65 109 696.4 0 109 66 80 696.4 0 80 67 252 696.4 0 252 68 85 696.4 0 85 69 99 696.4 0 99 70 144 696.4 0 144 71 135 696.4 0 135 72 130 696.4 0 130 73 172 696.4 0 172 74 173 696.4 0 173 75 321 696.4 0 321 76 1420 696.4 0 1420 77 965 696.4 0 965 78 1302 696.4 0 1302 79 441 696.4 0 441 80 1225 696.4 0 1225 81 313 696.4 0 313 82 382 696.4 0 382 83 171 696.4 0 171 84 207 696.4 0 207 85 226 696.4 0 226 86 373 696.4 0 373 87 1881 696.4 0 1881 88 3005 696.4 0 3005 89 162 696.4 0 162 90 140 696.4 0 140 91 128 696.4 0 128 92 155 696.4 0 155 93 139 696.4 0 139 94 124 696.4 0 124 95 163 696.4 0 163 96 128 696.4 0 128 97 153 696.4 0 153 98 174 696.4 0 174 99 181 696.4 0 181 100 240 696.4 0 240 101 300 696.4 0 300 102 151 696.4 0 151 103 117 696.4 0 117 104 158 696.4 0 158 105 124 696.4 0 124 106 150 696.4 0 150 107 174 696.4 0 174 108 191 696.4 0 191 109 151 696.4 0 151 110 323 696.4 0 323 111 173 696.4 0 173 112 201 696.4 0 201 113 197 696.4 0 197 114 187 696.4 0 187 115 195 696.4 0 195 116 198 696.4 0 198 117 257 696.4 0 257 118 187 696.4 0 187 119 185 696.4 0 185 120 174 696.4 0 174 121 206 696.4 0 206 122 271 696.4 0 271 123 419 696.4 0 419 124 413 696.4 0 413 125 271 696.4 0 271 126 260 696.4 0 260 127 329 696.4 0 329 128 361 696.4 0 361 129 566 696.4 0 566 130 5186 696.4 0 5186 131 3064 696.4 0 3064 132 3978 696.4 0 3978 133 222 696.4 0 222 134 235 696.4 0 235 135 252 696.4 0 252 136 1178 696.4 0 1178 137 1983 696.4 0 1983 138 1558 696.4 0 1558 139 735 696.4 0 735 140 556 696.4 0 556 141 207 696.4 0 207 142 190 696.4 0 190 143 176 696.4 0 176 144 177 696.4 0 177 145 190 696.4 0 190 146 211 696.4 0 211 147 215 696.4 0 215 148 239 696.4 0 239 149 408 696.4 0 408 150 165 696.4 0 165 151 213 696.4 0 213 152 212 696.4 0 212 153 210 696.4 0 210 154 170 696.4 0 170 155 152 696.4 0 152 156 187 696.4 0 187 157 162 696.4 0 162 158 181 696.4 0 181 159 136 696.4 0 136 160 148 696.4 0 148 161 175 696.4 0 175 162 256 696.4 0 256 163 203 696.4 0 203 164 150 696.4 0 150 165 210 696.4 0 210 166 191 696.4 0 191 167 173 696.4 0 173 168 172 696.4 0 172 169 198 696.4 0 198 170 289 696.4 0 289 171 161 696.4 0 161 172 170 696.4 0 170 173 163 696.4 0 163 174 142 696.4 0 142 175 208 696.4 0 208 176 202 696.4 0 202 177 183 696.4 0 183 178 205 696.4 0 205 179 151 696.4 0 151 180 162 696.4 0 162 181 142 696.4 0 142 182 145 696.4 0 145 183 188 696.4 0 188 184 141 696.4 0 141 185 198 696.4 0 198 186 207 696.4 0 207 187 264 696.4 0 264 188 206 696.4 0 206 189 205 696.4 0 205 190 187 696.4 0 187 191 232 696.4 0 232 192 197 696.4 0 197 193 192 696.4 0 192 194 186 696.4 0 186 195 240 696.4 0 240 196 350 696.4 0 350 197 293 696.4 0 293 198 231 696.4 0 231 199 168 696.4 0 168 200 166 696.4 0 166 201 176 696.4 0 176 202 177 696.4 0 177 203 187 696.4 0 187 204 149 696.4 0 149 205 181 696.4 0 181 206 164 696.4 0 164 207 192 696.4 0 192 208 163 696.4 0 163 209 193 696.4 0 193 210 193 696.4 0 193 211 187 696.4 0 187 212 193 696.4 0 193 213 216 696.4 0 216 214 217 696.4 0 217 215 183 696.4 0 183 216 206 696.4 0 206 217 212 696.4 0 212 218 185 696.4 0 185 219 225 696.4 0 225 220 235 696.4 0 235 221 258 696.4 0 258 222 375 696.4 0 375 223 331 696.4 0 331 224 369 696.4 0 369 225 294 696.4 0 294 226 382 696.4 0 382 227 513 696.4 0 513 228 376 696.4 0 376 229 362 696.4 0 362 230 400 696.4 0 400 231 635 696.4 0 635 232 550 696.4 0 550 233 721 696.4 0 721 234 283 696.4 0 283 235 271 696.4 0 271 236 263 696.4 0 263 237 327 696.4 0 327 238 381 696.4 0 381 239 475 696.4 0 475 240 582 696.4 0 582 241 478 696.4 0 478 242 719 696.4 0 719 243 523 696.4 0 523 244 878 696.4 0 878 245 550 696.4 0 550 246 501 696.4 0 501 247 408 696.4 0 408 248 414 696.4 0 414 249 383 696.4 0 383 250 550 696.4 0 550 251 3574 696.4 0 3574 252 3203 696.4 0 3203 253 5553 696.4 0 5553 254 328 696.4 0 328 255 293 696.4 0 293 256 835 696.4 0 835 257 742 696.4 0 742 258 1378 696.4 0 1378 259 461 696.4 0 461 260 451 696.4 0 451 261 255 696.4 0 255 262 284 696.4 0 284 263 208 696.4 0 208 264 233 696.4 0 233 265 251 696.4 0 251 266 189 696.4 0 189 267 173 696.4 0 173 268 175 696.4 0 175 269 229 696.4 0 229 270 257 696.4 0 257 271 273 696.4 0 273 272 220 696.4 0 220 273 213 696.4 0 213 274 196 696.4 0 196 275 194 696.4 0 194 276 315 696.4 0 315 277 217 696.4 0 217 278 276 696.4 0 276 279 240 696.4 0 240 280 206 696.4 0 206 281 249 696.4 0 249 282 213 696.4 0 213 283 335 696.4 0 335 284 374 696.4 0 374 285 477 696.4 0 477 286 357 696.4 0 357 287 211 696.4 0 211 288 194 696.4 0 194 289 194 696.4 0 194 290 242 696.4 0 242 291 239 696.4 0 239 292 304 696.4 0 304 293 255 696.4 0 255 294 224 696.4 0 224 295 326 696.4 0 326 296 339 696.4 0 339 297 777 696.4 0 777 298 534 696.4 0 534 299 482 696.4 0 482 300 326 696.4 0 326 301 224 696.4 0 224 Finished in 11.06 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 234,683 Reads with adapters: 41,036 (17.5%) Reads that were too short: 235 (0.1%) Reads written (passing filters): 40,801 (17.4%) Total basepairs processed: 61,089,897 bp Total written (filtered): 11,082,584 bp (18.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 41036 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 29 0.0 3 0 0 3 15 11 19 394 0.0 3 0 215 67 75 37 20 39458 0.0 4 36181 2521 556 152 48 21 139 0.0 4 20 95 14 7 3 22 15 0.0 4 3 0 7 3 2 23 226 0.0 4 5 3 3 129 86 24 3 0.0 4 0 0 0 0 3 26 2 0.0 4 1 0 0 0 1 30 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 38 1 0.0 4 1 46 1 0.0 4 0 0 0 1 47 1 0.0 4 0 0 0 0 1 54 17 0.0 4 0 0 0 0 17 60 1 0.0 4 0 0 0 0 1 62 2 0.0 4 0 0 0 0 2 63 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 66 4 0.0 4 0 0 0 0 4 67 2 0.0 4 0 0 0 0 2 68 1 0.0 4 0 0 0 0 1 69 2 0.0 4 0 0 0 0 2 70 4 0.0 4 0 0 0 0 4 71 1 0.0 4 0 0 0 0 1 72 3 0.0 4 0 0 0 0 3 73 2 0.0 4 0 0 0 0 2 74 1 0.0 4 0 0 0 0 1 75 2 0.0 4 0 0 0 0 2 76 4 0.0 4 0 0 0 0 4 77 1 0.0 4 0 0 0 1 78 2 0.0 4 0 0 0 0 2 79 8 0.0 4 0 0 0 2 6 80 1 0.0 4 0 0 0 0 1 81 5 0.0 4 0 0 0 0 5 82 3 0.0 4 0 0 0 0 3 83 3 0.0 4 1 0 0 0 2 84 1 0.0 4 0 0 0 0 1 85 2 0.0 4 0 0 0 0 2 87 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 93 2 0.0 4 0 0 0 0 2 94 2 0.0 4 0 0 0 0 2 97 1 0.0 4 0 0 0 0 1 99 12 0.0 4 0 0 0 1 11 100 6 0.0 4 0 0 0 0 6 102 1 0.0 4 0 0 0 0 1 104 2 0.0 4 0 0 0 0 2 107 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 0 2 113 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 115 1 0.0 4 1 116 2 0.0 4 0 0 0 0 2 117 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 119 2 0.0 4 0 0 0 0 2 121 8 0.0 4 0 0 0 0 8 122 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 0 0 0 2 126 3 0.0 4 0 0 0 0 3 127 384 0.0 4 0 0 0 0 384 128 3 0.0 4 0 0 0 1 2 129 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 137 3 0.0 4 0 0 0 0 3 138 2 0.0 4 0 0 0 0 2 139 2 0.0 4 0 0 0 0 2 140 1 0.0 4 0 0 0 0 1 142 2 0.0 4 0 0 0 0 2 143 2 0.0 4 0 0 0 0 2 144 2 0.0 4 0 0 0 0 2 145 1 0.0 4 0 0 0 0 1 146 2 0.0 4 0 0 0 0 2 147 2 0.0 4 0 0 0 0 2 149 1 0.0 4 0 0 0 0 1 150 3 0.0 4 1 0 0 0 2 151 1 0.0 4 0 0 0 0 1 152 3 0.0 4 0 0 0 0 3 153 7 0.0 4 1 0 0 1 5 154 2 0.0 4 0 2 155 3 0.0 4 1 1 1 156 3 0.0 4 1 0 0 0 2 157 3 0.0 4 0 0 0 1 2 158 1 0.0 4 0 0 0 0 1 159 6 0.0 4 0 0 0 0 6 160 8 0.0 4 0 0 0 0 8 161 1 0.0 4 0 0 0 0 1 163 3 0.0 4 0 0 0 0 3 168 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 171 2 0.0 4 0 0 0 0 2 172 3 0.0 4 0 0 0 0 3 173 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 176 2 0.0 4 0 0 0 0 2 178 2 0.0 4 0 0 0 1 1 179 1 0.0 4 0 0 0 0 1 180 4 0.0 4 0 0 0 0 4 182 3 0.0 4 0 0 0 0 3 183 4 0.0 4 0 0 0 0 4 184 1 0.0 4 0 0 0 0 1 185 2 0.0 4 0 0 0 0 2 186 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 194 3 0.0 4 0 0 0 0 3 195 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 200 3 0.0 4 0 0 0 0 3 203 1 0.0 4 0 0 0 0 1 204 4 0.0 4 0 0 0 0 4 205 4 0.0 4 0 0 0 0 4 206 3 0.0 4 0 0 0 1 2 207 1 0.0 4 0 0 0 0 1 208 4 0.0 4 0 0 0 0 4 209 1 0.0 4 0 0 0 0 1 210 2 0.0 4 0 0 0 1 1 212 3 0.0 4 0 0 0 0 3 213 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 2 0.0 4 0 0 0 0 2 216 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 218 3 0.0 4 0 0 0 0 3 219 2 0.0 4 0 0 0 0 2 220 1 0.0 4 0 0 0 0 1 221 2 0.0 4 0 0 0 0 2 223 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 225 4 0.0 4 0 0 0 0 4 226 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 8 0.0 4 0 0 0 1 7 229 3 0.0 4 0 0 0 0 3 232 1 0.0 4 0 0 0 0 1 233 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 235 4 0.0 4 0 0 0 0 4 236 3 0.0 4 0 0 0 0 3 237 1 0.0 4 0 0 0 0 1 238 4 0.0 4 0 0 0 1 3 239 1 0.0 4 0 0 0 0 1 240 1 0.0 4 0 0 0 0 1 241 1 0.0 4 0 0 0 0 1 242 1 0.0 4 0 0 0 0 1 243 2 0.0 4 0 0 0 1 1 245 1 0.0 4 0 0 0 0 1 248 2 0.0 4 0 0 0 0 2 249 1 0.0 4 0 0 0 0 1 250 1 0.0 4 0 0 0 0 1 251 3 0.0 4 0 0 0 0 3 254 2 0.0 4 0 0 0 0 2 255 1 0.0 4 0 0 0 0 1 257 3 0.0 4 0 0 0 0 3 258 4 0.0 4 0 0 0 0 4 259 1 0.0 4 0 0 0 0 1 260 2 0.0 4 0 0 0 0 2 262 2 0.0 4 0 0 0 0 2 264 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 266 3 0.0 4 0 0 0 0 3 267 2 0.0 4 0 0 0 0 2 271 1 0.0 4 0 0 0 0 1 272 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 0 1 276 3 0.0 4 0 0 0 0 3 278 1 0.0 4 0 0 0 0 1 279 1 0.0 4 0 0 0 0 1 282 5 0.0 4 0 0 0 2 3 283 2 0.0 4 0 0 0 0 2 291 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 293 3 0.0 4 0 0 0 0 3 294 5 0.0 4 0 0 0 0 5 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.46 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 40,801 Reads with adapters: 7,369 (18.1%) Reads written (passing filters): 40,801 (100.0%) Total basepairs processed: 11,082,584 bp Total written (filtered): 11,000,664 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7369 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.4% C: 8.8% G: 85.4% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 51 637.5 0 51 4 12 159.4 0 12 6 2 10.0 0 2 9 90 0.2 0 5 85 10 2810 0.0 1 249 2561 11 4275 0.0 1 170 4105 12 79 0.0 1 1 78 13 18 0.0 1 0 18 14 7 0.0 1 0 7 15 1 0.0 1 0 1 115 1 0.0 1 0 1 149 1 0.0 1 1 151 5 0.0 1 3 2 153 1 0.0 1 0 1 190 3 0.0 1 1 2 209 10 0.0 1 7 3 211 2 0.0 1 1 1 223 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.