This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACGCGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.26 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 387,050 (13.6%) Reads that were too short: 345,317 (12.1%) Reads written (passing filters): 41,733 (1.5%) Total basepairs processed: 858,556,748 bp Total written (filtered): 9,172,943 bp (1.1%) === Adapter 1 === Sequence: ACGCGC; Type: regular 5'; Length: 6; Trimmed: 387050 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1006 696.4 0 1006 7 11392 696.4 0 11392 8 273 696.4 0 273 9 9409 696.4 0 9409 10 44 696.4 0 44 11 22 696.4 0 22 12 18 696.4 0 18 13 24 696.4 0 24 14 14 696.4 0 14 15 14 696.4 0 14 16 15 696.4 0 15 17 15 696.4 0 15 18 19 696.4 0 19 19 21 696.4 0 21 20 24 696.4 0 24 21 26 696.4 0 26 22 20 696.4 0 20 23 24 696.4 0 24 24 11 696.4 0 11 25 9 696.4 0 9 26 11 696.4 0 11 27 15 696.4 0 15 28 17 696.4 0 17 29 8 696.4 0 8 30 15 696.4 0 15 31 16 696.4 0 16 32 10 696.4 0 10 33 14 696.4 0 14 34 15 696.4 0 15 35 19 696.4 0 19 36 16 696.4 0 16 37 12 696.4 0 12 38 15 696.4 0 15 39 26 696.4 0 26 40 21 696.4 0 21 41 19 696.4 0 19 42 28 696.4 0 28 43 19 696.4 0 19 44 12 696.4 0 12 45 25 696.4 0 25 46 14 696.4 0 14 47 28 696.4 0 28 48 20 696.4 0 20 49 38 696.4 0 38 50 23 696.4 0 23 51 27 696.4 0 27 52 30 696.4 0 30 53 57 696.4 0 57 54 51 696.4 0 51 55 58 696.4 0 58 56 41 696.4 0 41 57 20 696.4 0 20 58 22 696.4 0 22 59 23 696.4 0 23 60 23 696.4 0 23 61 27 696.4 0 27 62 26 696.4 0 26 63 31 696.4 0 31 64 24 696.4 0 24 65 19 696.4 0 19 66 34 696.4 0 34 67 26 696.4 0 26 68 22 696.4 0 22 69 16 696.4 0 16 70 25 696.4 0 25 71 23 696.4 0 23 72 44 696.4 0 44 73 34 696.4 0 34 74 51 696.4 0 51 75 19 696.4 0 19 76 21 696.4 0 21 77 18 696.4 0 18 78 13 696.4 0 13 79 21 696.4 0 21 80 20 696.4 0 20 81 24 696.4 0 24 82 24 696.4 0 24 83 27 696.4 0 27 84 34 696.4 0 34 85 33 696.4 0 33 86 28 696.4 0 28 87 38 696.4 0 38 88 20 696.4 0 20 89 14 696.4 0 14 90 18 696.4 0 18 91 26 696.4 0 26 92 26 696.4 0 26 93 25 696.4 0 25 94 17 696.4 0 17 95 31 696.4 0 31 96 21 696.4 0 21 97 19 696.4 0 19 98 21 696.4 0 21 99 28 696.4 0 28 100 22 696.4 0 22 101 24 696.4 0 24 102 20 696.4 0 20 103 24 696.4 0 24 104 24 696.4 0 24 105 21 696.4 0 21 106 18 696.4 0 18 107 24 696.4 0 24 108 25 696.4 0 25 109 22 696.4 0 22 110 20 696.4 0 20 111 15 696.4 0 15 112 30 696.4 0 30 113 39 696.4 0 39 114 31 696.4 0 31 115 43 696.4 0 43 116 80 696.4 0 80 117 36 696.4 0 36 118 34 696.4 0 34 119 35 696.4 0 35 120 35 696.4 0 35 121 33 696.4 0 33 122 54 696.4 0 54 123 86 696.4 0 86 124 92 696.4 0 92 125 251 696.4 0 251 126 121 696.4 0 121 127 53 696.4 0 53 128 187 696.4 0 187 129 45 696.4 0 45 130 32 696.4 0 32 131 34 696.4 0 34 132 25 696.4 0 25 133 15 696.4 0 15 134 34 696.4 0 34 135 24 696.4 0 24 136 28 696.4 0 28 137 30 696.4 0 30 138 57 696.4 0 57 139 30 696.4 0 30 140 29 696.4 0 29 141 28 696.4 0 28 142 24 696.4 0 24 143 32 696.4 0 32 144 36 696.4 0 36 145 36 696.4 0 36 146 26 696.4 0 26 147 37 696.4 0 37 148 24 696.4 0 24 149 48 696.4 0 48 150 135 696.4 0 135 151 111 696.4 0 111 152 163 696.4 0 163 153 43 696.4 0 43 154 46 696.4 0 46 155 35 696.4 0 35 156 39 696.4 0 39 157 27 696.4 0 27 158 36 696.4 0 36 159 47 696.4 0 47 160 118 696.4 0 118 161 70 696.4 0 70 162 118 696.4 0 118 163 38 696.4 0 38 164 85 696.4 0 85 165 432 696.4 0 432 166 258 696.4 0 258 167 372 696.4 0 372 168 159 696.4 0 159 169 64 696.4 0 64 170 59 696.4 0 59 171 48 696.4 0 48 172 53 696.4 0 53 173 59 696.4 0 59 174 36 696.4 0 36 175 39 696.4 0 39 176 45 696.4 0 45 177 50 696.4 0 50 178 63 696.4 0 63 179 87 696.4 0 87 180 66 696.4 0 66 181 78 696.4 0 78 182 91 696.4 0 91 183 104 696.4 0 104 184 132 696.4 0 132 185 546 696.4 0 546 186 489 696.4 0 489 187 5734 696.4 0 5734 188 2045 696.4 0 2045 189 1905 696.4 0 1905 190 49 696.4 0 49 191 31 696.4 0 31 192 37 696.4 0 37 193 27 696.4 0 27 194 60 696.4 0 60 195 70 696.4 0 70 196 52 696.4 0 52 197 54 696.4 0 54 198 67 696.4 0 67 199 111 696.4 0 111 200 573 696.4 0 573 201 108 696.4 0 108 202 56 696.4 0 56 203 96 696.4 0 96 204 55 696.4 0 55 205 39 696.4 0 39 206 34 696.4 0 34 207 96 696.4 0 96 208 47 696.4 0 47 209 73 696.4 0 73 210 409 696.4 0 409 211 67 696.4 0 67 212 45 696.4 0 45 213 65 696.4 0 65 214 58 696.4 0 58 215 49 696.4 0 49 216 81 696.4 0 81 217 197 696.4 0 197 218 202 696.4 0 202 219 391 696.4 0 391 220 933 696.4 0 933 221 2312 696.4 0 2312 222 6269 696.4 0 6269 223 4865 696.4 0 4865 224 2660 696.4 0 2660 225 466 696.4 0 466 226 290 696.4 0 290 227 410 696.4 0 410 228 123 696.4 0 123 229 233 696.4 0 233 230 320 696.4 0 320 231 444 696.4 0 444 232 933 696.4 0 933 233 1152 696.4 0 1152 234 2003 696.4 0 2003 235 4060 696.4 0 4060 236 7072 696.4 0 7072 237 112280 696.4 0 112280 238 103206 696.4 0 103206 239 76394 696.4 0 76394 240 4100 696.4 0 4100 241 594 696.4 0 594 242 80 696.4 0 80 243 58 696.4 0 58 244 127 696.4 0 127 245 1156 696.4 0 1156 246 741 696.4 0 741 247 2235 696.4 0 2235 248 948 696.4 0 948 249 513 696.4 0 513 250 227 696.4 0 227 251 83 696.4 0 83 252 42 696.4 0 42 253 17 696.4 0 17 254 23 696.4 0 23 255 34 696.4 0 34 256 20 696.4 0 20 257 36 696.4 0 36 258 26 696.4 0 26 259 19 696.4 0 19 260 43 696.4 0 43 261 49 696.4 0 49 262 29 696.4 0 29 263 33 696.4 0 33 264 35 696.4 0 35 265 37 696.4 0 37 266 60 696.4 0 60 267 165 696.4 0 165 268 398 696.4 0 398 269 495 696.4 0 495 270 387 696.4 0 387 271 154 696.4 0 154 272 71 696.4 0 71 273 84 696.4 0 84 274 94 696.4 0 94 275 59 696.4 0 59 276 58 696.4 0 58 277 56 696.4 0 56 278 67 696.4 0 67 279 63 696.4 0 63 280 78 696.4 0 78 281 176 696.4 0 176 282 174 696.4 0 174 283 243 696.4 0 243 284 46 696.4 0 46 285 66 696.4 0 66 286 71 696.4 0 71 287 183 696.4 0 183 288 184 696.4 0 184 289 259 696.4 0 259 290 227 696.4 0 227 291 130 696.4 0 130 292 57 696.4 0 57 293 60 696.4 0 60 294 346 696.4 0 346 295 295 696.4 0 295 296 365 696.4 0 365 297 173 696.4 0 173 298 47 696.4 0 47 299 55 696.4 0 55 300 37 696.4 0 37 301 44 696.4 0 44 Finished in 1.77 s (42 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 41,733 Reads with adapters: 9,920 (23.8%) Reads that were too short: 30 (0.1%) Reads written (passing filters): 9,890 (23.7%) Total basepairs processed: 9,172,943 bp Total written (filtered): 2,712,695 bp (29.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9920 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 35 0.0 3 0 35 17 9757 0.0 3 9522 222 12 1 18 27 0.0 3 4 23 19 4 0.0 3 0 0 4 20 9 0.0 3 0 0 0 9 21 10 0.0 3 0 0 0 10 22 1 0.0 3 0 0 0 1 24 2 0.0 3 0 0 0 2 32 25 0.0 3 0 0 1 24 35 1 0.0 3 1 41 1 0.0 3 1 42 1 0.0 3 0 0 0 1 48 7 0.0 3 0 0 0 7 51 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 118 5 0.0 3 0 0 0 5 119 1 0.0 3 0 0 0 1 121 4 0.0 3 0 0 0 4 127 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 280 3 0.0 3 0 0 0 3 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.35 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 9,890 Reads with adapters: 618 (6.2%) Reads written (passing filters): 9,890 (100.0%) Total basepairs processed: 2,712,695 bp Total written (filtered): 2,710,825 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 618 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 84.1% C: 4.9% G: 10.0% T: 1.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 604 154.5 0 604 4 13 38.6 0 13 6 1 2.4 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.