This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 144.17 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 424,229 (14.4%) Reads that were too short: 9,363 (0.3%) Reads written (passing filters): 414,866 (14.1%) Total basepairs processed: 887,907,860 bp Total written (filtered): 72,596,688 bp (8.2%) === Adapter 1 === Sequence: CCTAAT; Type: regular 5'; Length: 6; Trimmed: 424229 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 474 720.2 0 474 7 33859 720.2 0 33859 8 2471 720.2 0 2471 9 26865 720.2 0 26865 10 73 720.2 0 73 11 26 720.2 0 26 12 36 720.2 0 36 13 108 720.2 0 108 14 141 720.2 0 141 15 94 720.2 0 94 16 62 720.2 0 62 17 38 720.2 0 38 18 39 720.2 0 39 19 54 720.2 0 54 20 46 720.2 0 46 21 52 720.2 0 52 22 25 720.2 0 25 23 37 720.2 0 37 24 47 720.2 0 47 25 70 720.2 0 70 26 114 720.2 0 114 27 631 720.2 0 631 28 676 720.2 0 676 29 1301 720.2 0 1301 30 38 720.2 0 38 31 90 720.2 0 90 32 37 720.2 0 37 33 38 720.2 0 38 34 58 720.2 0 58 35 55 720.2 0 55 36 40 720.2 0 40 37 83 720.2 0 83 38 33 720.2 0 33 39 29 720.2 0 29 40 51 720.2 0 51 41 48 720.2 0 48 42 65 720.2 0 65 43 59 720.2 0 59 44 72 720.2 0 72 45 59 720.2 0 59 46 44 720.2 0 44 47 56 720.2 0 56 48 62 720.2 0 62 49 81 720.2 0 81 50 74 720.2 0 74 51 80 720.2 0 80 52 107 720.2 0 107 53 68 720.2 0 68 54 44 720.2 0 44 55 57 720.2 0 57 56 45 720.2 0 45 57 142 720.2 0 142 58 268 720.2 0 268 59 504 720.2 0 504 60 58 720.2 0 58 61 48 720.2 0 48 62 43 720.2 0 43 63 52 720.2 0 52 64 43 720.2 0 43 65 69 720.2 0 69 66 44 720.2 0 44 67 48 720.2 0 48 68 46 720.2 0 46 69 54 720.2 0 54 70 50 720.2 0 50 71 50 720.2 0 50 72 40 720.2 0 40 73 58 720.2 0 58 74 48 720.2 0 48 75 54 720.2 0 54 76 64 720.2 0 64 77 61 720.2 0 61 78 59 720.2 0 59 79 61 720.2 0 61 80 67 720.2 0 67 81 133 720.2 0 133 82 42 720.2 0 42 83 49 720.2 0 49 84 61 720.2 0 61 85 74 720.2 0 74 86 44 720.2 0 44 87 45 720.2 0 45 88 58 720.2 0 58 89 74 720.2 0 74 90 82 720.2 0 82 91 181 720.2 0 181 92 96 720.2 0 96 93 115 720.2 0 115 94 110 720.2 0 110 95 158 720.2 0 158 96 167 720.2 0 167 97 84 720.2 0 84 98 82 720.2 0 82 99 63 720.2 0 63 100 83 720.2 0 83 101 118 720.2 0 118 102 138 720.2 0 138 103 198 720.2 0 198 104 325 720.2 0 325 105 391 720.2 0 391 106 753 720.2 0 753 107 975 720.2 0 975 108 16987 720.2 0 16987 109 8887 720.2 0 8887 110 26860 720.2 0 26860 111 230 720.2 0 230 112 73 720.2 0 73 113 66 720.2 0 66 114 55 720.2 0 55 115 69 720.2 0 69 116 104 720.2 0 104 117 94 720.2 0 94 118 91 720.2 0 91 119 64 720.2 0 64 120 127 720.2 0 127 121 182 720.2 0 182 122 98 720.2 0 98 123 101 720.2 0 101 124 148 720.2 0 148 125 130 720.2 0 130 126 101 720.2 0 101 127 152 720.2 0 152 128 214 720.2 0 214 129 241 720.2 0 241 130 168 720.2 0 168 131 259 720.2 0 259 132 276 720.2 0 276 133 366 720.2 0 366 134 5790 720.2 0 5790 135 6187 720.2 0 6187 136 11783 720.2 0 11783 137 409 720.2 0 409 138 159 720.2 0 159 139 96 720.2 0 96 140 148 720.2 0 148 141 225 720.2 0 225 142 272 720.2 0 272 143 291 720.2 0 291 144 344 720.2 0 344 145 494 720.2 0 494 146 1103 720.2 0 1103 147 2218 720.2 0 2218 148 455 720.2 0 455 149 677 720.2 0 677 150 842 720.2 0 842 151 404 720.2 0 404 152 444 720.2 0 444 153 942 720.2 0 942 154 1054 720.2 0 1054 155 3239 720.2 0 3239 156 1574 720.2 0 1574 157 4416 720.2 0 4416 158 2451 720.2 0 2451 159 3597 720.2 0 3597 160 56399 720.2 0 56399 161 108688 720.2 0 108688 162 65339 720.2 0 65339 163 315 720.2 0 315 164 104 720.2 0 104 165 90 720.2 0 90 166 80 720.2 0 80 167 74 720.2 0 74 168 51 720.2 0 51 169 56 720.2 0 56 170 55 720.2 0 55 171 68 720.2 0 68 172 41 720.2 0 41 173 50 720.2 0 50 174 75 720.2 0 75 175 75 720.2 0 75 176 89 720.2 0 89 177 49 720.2 0 49 178 79 720.2 0 79 179 95 720.2 0 95 180 103 720.2 0 103 181 94 720.2 0 94 182 60 720.2 0 60 183 69 720.2 0 69 184 56 720.2 0 56 185 56 720.2 0 56 186 52 720.2 0 52 187 62 720.2 0 62 188 49 720.2 0 49 189 59 720.2 0 59 190 44 720.2 0 44 191 226 720.2 0 226 192 111 720.2 0 111 193 181 720.2 0 181 194 58 720.2 0 58 195 50 720.2 0 50 196 43 720.2 0 43 197 35 720.2 0 35 198 44 720.2 0 44 199 49 720.2 0 49 200 42 720.2 0 42 201 52 720.2 0 52 202 63 720.2 0 63 203 73 720.2 0 73 204 83 720.2 0 83 205 69 720.2 0 69 206 44 720.2 0 44 207 55 720.2 0 55 208 83 720.2 0 83 209 62 720.2 0 62 210 44 720.2 0 44 211 38 720.2 0 38 212 38 720.2 0 38 213 31 720.2 0 31 214 46 720.2 0 46 215 47 720.2 0 47 216 49 720.2 0 49 217 53 720.2 0 53 218 80 720.2 0 80 219 78 720.2 0 78 220 56 720.2 0 56 221 137 720.2 0 137 222 358 720.2 0 358 223 187 720.2 0 187 224 146 720.2 0 146 225 77 720.2 0 77 226 61 720.2 0 61 227 54 720.2 0 54 228 59 720.2 0 59 229 46 720.2 0 46 230 60 720.2 0 60 231 63 720.2 0 63 232 93 720.2 0 93 233 77 720.2 0 77 234 101 720.2 0 101 235 104 720.2 0 104 236 138 720.2 0 138 237 148 720.2 0 148 238 40 720.2 0 40 239 41 720.2 0 41 240 29 720.2 0 29 241 55 720.2 0 55 242 89 720.2 0 89 243 94 720.2 0 94 244 44 720.2 0 44 245 44 720.2 0 44 246 39 720.2 0 39 247 52 720.2 0 52 248 63 720.2 0 63 249 61 720.2 0 61 250 130 720.2 0 130 251 297 720.2 0 297 252 379 720.2 0 379 253 644 720.2 0 644 254 458 720.2 0 458 255 232 720.2 0 232 256 258 720.2 0 258 257 78 720.2 0 78 258 47 720.2 0 47 259 36 720.2 0 36 260 46 720.2 0 46 261 77 720.2 0 77 262 95 720.2 0 95 263 121 720.2 0 121 264 51 720.2 0 51 265 84 720.2 0 84 266 57 720.2 0 57 267 68 720.2 0 68 268 43 720.2 0 43 269 76 720.2 0 76 270 93 720.2 0 93 271 102 720.2 0 102 272 82 720.2 0 82 273 44 720.2 0 44 274 40 720.2 0 40 275 49 720.2 0 49 276 39 720.2 0 39 277 73 720.2 0 73 278 105 720.2 0 105 279 121 720.2 0 121 280 117 720.2 0 117 281 98 720.2 0 98 282 130 720.2 0 130 283 227 720.2 0 227 284 116 720.2 0 116 285 53 720.2 0 53 286 39 720.2 0 39 287 45 720.2 0 45 288 37 720.2 0 37 289 41 720.2 0 41 290 56 720.2 0 56 291 70 720.2 0 70 292 82 720.2 0 82 293 82 720.2 0 82 294 49 720.2 0 49 295 41 720.2 0 41 296 64 720.2 0 64 297 69 720.2 0 69 298 78 720.2 0 78 299 65 720.2 0 65 300 52 720.2 0 52 301 75 720.2 0 75 Finished in 24.71 s (60 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 414,866 Reads with adapters: 30,267 (7.3%) Reads that were too short: 61 (0.0%) Reads written (passing filters): 30,206 (7.3%) Total basepairs processed: 72,596,688 bp Total written (filtered): 8,163,254 bp (11.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 30267 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 6 0.0 3 0 0 0 2 4 18 10 0.0 3 0 0 3 4 3 19 103 0.0 3 0 78 9 12 4 20 29424 0.0 4 27967 1169 210 59 19 21 67 0.0 4 5 51 5 5 1 22 8 0.0 4 1 0 7 23 64 0.0 4 0 0 0 39 25 24 1 0.0 4 0 0 0 0 1 26 1 0.0 4 1 31 1 0.0 4 1 32 1 0.0 4 1 38 2 0.0 4 1 0 0 0 1 44 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 61 2 0.0 4 0 0 0 0 2 64 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 77 12 0.0 4 0 0 0 0 12 80 6 0.0 4 0 0 0 0 6 81 8 0.0 4 0 0 0 0 8 82 6 0.0 4 0 0 0 1 5 94 1 0.0 4 0 1 104 3 0.0 4 0 0 0 0 3 106 1 0.0 4 0 0 0 1 107 1 0.0 4 0 0 0 0 1 108 2 0.0 4 0 0 0 0 2 119 1 0.0 4 0 0 0 1 122 4 0.0 4 0 0 0 0 4 125 1 0.0 4 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 492 0.0 4 0 0 0 0 492 130 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 265 2 0.0 4 0 0 0 0 2 266 1 0.0 4 0 0 0 1 267 2 0.0 4 0 0 0 0 2 275 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 294 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.73 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 30,206 Reads with adapters: 9,308 (30.8%) Reads written (passing filters): 30,206 (100.0%) Total basepairs processed: 8,163,254 bp Total written (filtered): 8,071,148 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 9308 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.5% C: 5.7% G: 91.4% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 15 472.0 0 15 4 4 118.0 0 4 7 3 1.8 0 3 8 3 0.5 0 3 9 5600 0.1 0 335 5265 10 2745 0.0 1 144 2601 11 884 0.0 1 12 872 12 26 0.0 1 0 26 13 5 0.0 1 0 5 14 1 0.0 1 0 1 72 1 0.0 1 1 121 1 0.0 1 0 1 122 1 0.0 1 1 148 1 0.0 1 1 150 3 0.0 1 2 1 151 1 0.0 1 1 208 13 0.0 1 12 1 267 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.