This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATCTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 145.17 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 371,064 (12.6%) Reads that were too short: 9,533 (0.3%) Reads written (passing filters): 361,531 (12.3%) Total basepairs processed: 887,907,860 bp Total written (filtered): 68,548,259 bp (7.7%) === Adapter 1 === Sequence: TATCTG; Type: regular 5'; Length: 6; Trimmed: 371064 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 353 720.2 0 353 7 414 720.2 0 414 8 12432 720.2 0 12432 9 9873 720.2 0 9873 10 164 720.2 0 164 11 72 720.2 0 72 12 46 720.2 0 46 13 58 720.2 0 58 14 66 720.2 0 66 15 154 720.2 0 154 16 61 720.2 0 61 17 75 720.2 0 75 18 73 720.2 0 73 19 79 720.2 0 79 20 58 720.2 0 58 21 62 720.2 0 62 22 60 720.2 0 60 23 61 720.2 0 61 24 69 720.2 0 69 25 179 720.2 0 179 26 169 720.2 0 169 27 273 720.2 0 273 28 75 720.2 0 75 29 77 720.2 0 77 30 60 720.2 0 60 31 60 720.2 0 60 32 62 720.2 0 62 33 59 720.2 0 59 34 56 720.2 0 56 35 77 720.2 0 77 36 70 720.2 0 70 37 86 720.2 0 86 38 116 720.2 0 116 39 63 720.2 0 63 40 74 720.2 0 74 41 44 720.2 0 44 42 41 720.2 0 41 43 62 720.2 0 62 44 58 720.2 0 58 45 54 720.2 0 54 46 61 720.2 0 61 47 65 720.2 0 65 48 46 720.2 0 46 49 42 720.2 0 42 50 44 720.2 0 44 51 49 720.2 0 49 52 53 720.2 0 53 53 70 720.2 0 70 54 38 720.2 0 38 55 85 720.2 0 85 56 106 720.2 0 106 57 76 720.2 0 76 58 59 720.2 0 59 59 45 720.2 0 45 60 72 720.2 0 72 61 60 720.2 0 60 62 60 720.2 0 60 63 66 720.2 0 66 64 69 720.2 0 69 65 56 720.2 0 56 66 92 720.2 0 92 67 89 720.2 0 89 68 124 720.2 0 124 69 111 720.2 0 111 70 1234 720.2 0 1234 71 906 720.2 0 906 72 2521 720.2 0 2521 73 76 720.2 0 76 74 69 720.2 0 69 75 53 720.2 0 53 76 102 720.2 0 102 77 102 720.2 0 102 78 94 720.2 0 94 79 106 720.2 0 106 80 130 720.2 0 130 81 99 720.2 0 99 82 465 720.2 0 465 83 260 720.2 0 260 84 301 720.2 0 301 85 119 720.2 0 119 86 496 720.2 0 496 87 95 720.2 0 95 88 71 720.2 0 71 89 78 720.2 0 78 90 62 720.2 0 62 91 104 720.2 0 104 92 91 720.2 0 91 93 156 720.2 0 156 94 100 720.2 0 100 95 242 720.2 0 242 96 538 720.2 0 538 97 174 720.2 0 174 98 302 720.2 0 302 99 257 720.2 0 257 100 425 720.2 0 425 101 863 720.2 0 863 102 235 720.2 0 235 103 232 720.2 0 232 104 120 720.2 0 120 105 203 720.2 0 203 106 368 720.2 0 368 107 426 720.2 0 426 108 798 720.2 0 798 109 1303 720.2 0 1303 110 1458 720.2 0 1458 111 3409 720.2 0 3409 112 4243 720.2 0 4243 113 83430 720.2 0 83430 114 36624 720.2 0 36624 115 99133 720.2 0 99133 116 498 720.2 0 498 117 94 720.2 0 94 118 165 720.2 0 165 119 131 720.2 0 131 120 119 720.2 0 119 121 197 720.2 0 197 122 347 720.2 0 347 123 158 720.2 0 158 124 188 720.2 0 188 125 279 720.2 0 279 126 1091 720.2 0 1091 127 324 720.2 0 324 128 487 720.2 0 487 129 686 720.2 0 686 130 321 720.2 0 321 131 393 720.2 0 393 132 464 720.2 0 464 133 646 720.2 0 646 134 968 720.2 0 968 135 20185 720.2 0 20185 136 42886 720.2 0 42886 137 11200 720.2 0 11200 138 226 720.2 0 226 139 337 720.2 0 337 140 330 720.2 0 330 141 521 720.2 0 521 142 129 720.2 0 129 143 111 720.2 0 111 144 161 720.2 0 161 145 305 720.2 0 305 146 165 720.2 0 165 147 149 720.2 0 149 148 276 720.2 0 276 149 130 720.2 0 130 150 75 720.2 0 75 151 71 720.2 0 71 152 57 720.2 0 57 153 67 720.2 0 67 154 114 720.2 0 114 155 67 720.2 0 67 156 81 720.2 0 81 157 103 720.2 0 103 158 154 720.2 0 154 159 702 720.2 0 702 160 607 720.2 0 607 161 131 720.2 0 131 162 59 720.2 0 59 163 62 720.2 0 62 164 87 720.2 0 87 165 62 720.2 0 62 166 64 720.2 0 64 167 51 720.2 0 51 168 78 720.2 0 78 169 60 720.2 0 60 170 80 720.2 0 80 171 48 720.2 0 48 172 47 720.2 0 47 173 57 720.2 0 57 174 53 720.2 0 53 175 75 720.2 0 75 176 51 720.2 0 51 177 86 720.2 0 86 178 61 720.2 0 61 179 107 720.2 0 107 180 174 720.2 0 174 181 105 720.2 0 105 182 171 720.2 0 171 183 78 720.2 0 78 184 87 720.2 0 87 185 92 720.2 0 92 186 79 720.2 0 79 187 78 720.2 0 78 188 75 720.2 0 75 189 47 720.2 0 47 190 60 720.2 0 60 191 51 720.2 0 51 192 50 720.2 0 50 193 60 720.2 0 60 194 49 720.2 0 49 195 54 720.2 0 54 196 81 720.2 0 81 197 61 720.2 0 61 198 65 720.2 0 65 199 90 720.2 0 90 200 105 720.2 0 105 201 1379 720.2 0 1379 202 150 720.2 0 150 203 223 720.2 0 223 204 114 720.2 0 114 205 129 720.2 0 129 206 161 720.2 0 161 207 76 720.2 0 76 208 87 720.2 0 87 209 58 720.2 0 58 210 58 720.2 0 58 211 66 720.2 0 66 212 58 720.2 0 58 213 70 720.2 0 70 214 77 720.2 0 77 215 96 720.2 0 96 216 89 720.2 0 89 217 92 720.2 0 92 218 45 720.2 0 45 219 83 720.2 0 83 220 150 720.2 0 150 221 154 720.2 0 154 222 38 720.2 0 38 223 83 720.2 0 83 224 331 720.2 0 331 225 371 720.2 0 371 226 376 720.2 0 376 227 84 720.2 0 84 228 61 720.2 0 61 229 82 720.2 0 82 230 56 720.2 0 56 231 50 720.2 0 50 232 68 720.2 0 68 233 81 720.2 0 81 234 159 720.2 0 159 235 201 720.2 0 201 236 155 720.2 0 155 237 68 720.2 0 68 238 49 720.2 0 49 239 34 720.2 0 34 240 44 720.2 0 44 241 68 720.2 0 68 242 66 720.2 0 66 243 63 720.2 0 63 244 64 720.2 0 64 245 66 720.2 0 66 246 68 720.2 0 68 247 54 720.2 0 54 248 54 720.2 0 54 249 476 720.2 0 476 250 52 720.2 0 52 251 80 720.2 0 80 252 41 720.2 0 41 253 50 720.2 0 50 254 57 720.2 0 57 255 55 720.2 0 55 256 78 720.2 0 78 257 101 720.2 0 101 258 70 720.2 0 70 259 66 720.2 0 66 260 80 720.2 0 80 261 55 720.2 0 55 262 57 720.2 0 57 263 54 720.2 0 54 264 58 720.2 0 58 265 39 720.2 0 39 266 50 720.2 0 50 267 46 720.2 0 46 268 71 720.2 0 71 269 80 720.2 0 80 270 78 720.2 0 78 271 101 720.2 0 101 272 138 720.2 0 138 273 149 720.2 0 149 274 98 720.2 0 98 275 112 720.2 0 112 276 93 720.2 0 93 277 69 720.2 0 69 278 61 720.2 0 61 279 59 720.2 0 59 280 90 720.2 0 90 281 129 720.2 0 129 282 210 720.2 0 210 283 69 720.2 0 69 284 69 720.2 0 69 285 84 720.2 0 84 286 77 720.2 0 77 287 78 720.2 0 78 288 88 720.2 0 88 289 90 720.2 0 90 290 45 720.2 0 45 291 51 720.2 0 51 292 81 720.2 0 81 293 70 720.2 0 70 294 114 720.2 0 114 295 152 720.2 0 152 296 153 720.2 0 153 297 95 720.2 0 95 298 61 720.2 0 61 299 42 720.2 0 42 300 42 720.2 0 42 301 39 720.2 0 39 Finished in 22.34 s (62 us/read; 0.97 M reads/minute). === Summary === Total reads processed: 361,531 Reads with adapters: 10,369 (2.9%) Reads that were too short: 66 (0.0%) Reads written (passing filters): 10,303 (2.8%) Total basepairs processed: 68,548,259 bp Total written (filtered): 2,787,690 bp (4.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10369 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 0 2 1 19 42 0.0 3 0 34 6 2 20 10054 0.0 4 9587 391 55 14 7 21 39 0.0 4 13 22 2 2 22 1 0.0 4 0 0 0 1 23 18 0.0 4 2 0 0 10 6 25 1 0.0 4 1 26 2 0.0 4 1 0 1 28 1 0.0 4 1 38 1 0.0 4 1 41 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 80 1 0.0 4 1 82 2 0.0 4 0 2 83 4 0.0 4 0 0 0 1 3 84 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 97 2 0.0 4 0 0 0 0 2 101 1 0.0 4 0 1 103 2 0.0 4 0 0 0 0 2 121 2 0.0 4 0 0 0 0 2 122 25 0.0 4 0 0 0 0 25 123 9 0.0 4 0 0 0 2 7 124 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 127 0.0 4 0 0 0 0 127 129 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 4 0.0 4 0 0 0 0 4 162 2 0.0 4 0 0 0 0 2 163 1 0.0 4 0 0 0 0 1 165 2 0.0 4 0 0 0 0 2 167 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 1 211 1 0.0 4 0 0 0 1 225 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 1 264 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.62 s (60 us/read; 1.00 M reads/minute). === Summary === Total reads processed: 10,303 Reads with adapters: 3,109 (30.2%) Reads written (passing filters): 10,303 (100.0%) Total basepairs processed: 2,787,690 bp Total written (filtered): 2,655,516 bp (95.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3109 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.3% C: 5.0% G: 79.5% T: 14.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 161.0 0 3 8 2 0.2 0 2 9 1494 0.0 0 72 1422 10 993 0.0 1 72 921 11 206 0.0 1 8 198 12 3 0.0 1 0 3 13 4 0.0 1 0 4 135 1 0.0 1 0 1 150 2 0.0 1 1 1 189 1 0.0 1 0 1 208 10 0.0 1 9 1 245 1 0.0 1 0 1 248 1 0.0 1 1 264 1 0.0 1 0 1 265 2 0.0 1 2 266 373 0.0 1 351 22 267 8 0.0 1 8 268 2 0.0 1 2 269 2 0.0 1 2