This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GACGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 149.08 s (51 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 552,411 (18.7%) Reads that were too short: 83,900 (2.8%) Reads written (passing filters): 468,511 (15.9%) Total basepairs processed: 887,907,860 bp Total written (filtered): 90,548,868 bp (10.2%) === Adapter 1 === Sequence: GACGGC; Type: regular 5'; Length: 6; Trimmed: 552411 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 901 720.2 0 901 7 9695 720.2 0 9695 8 250 720.2 0 250 9 11171 720.2 0 11171 10 79 720.2 0 79 11 67 720.2 0 67 12 50 720.2 0 50 13 35 720.2 0 35 14 56 720.2 0 56 15 67 720.2 0 67 16 106 720.2 0 106 17 72 720.2 0 72 18 42 720.2 0 42 19 74 720.2 0 74 20 96 720.2 0 96 21 81 720.2 0 81 22 79 720.2 0 79 23 56 720.2 0 56 24 60 720.2 0 60 25 53 720.2 0 53 26 45 720.2 0 45 27 35 720.2 0 35 28 63 720.2 0 63 29 55 720.2 0 55 30 54 720.2 0 54 31 64 720.2 0 64 32 58 720.2 0 58 33 70 720.2 0 70 34 79 720.2 0 79 35 86 720.2 0 86 36 145 720.2 0 145 37 57 720.2 0 57 38 68 720.2 0 68 39 62 720.2 0 62 40 66 720.2 0 66 41 122 720.2 0 122 42 125 720.2 0 125 43 122 720.2 0 122 44 82 720.2 0 82 45 112 720.2 0 112 46 129 720.2 0 129 47 214 720.2 0 214 48 84 720.2 0 84 49 331 720.2 0 331 50 811 720.2 0 811 51 428 720.2 0 428 52 95 720.2 0 95 53 137 720.2 0 137 54 98 720.2 0 98 55 134 720.2 0 134 56 88 720.2 0 88 57 84 720.2 0 84 58 133 720.2 0 133 59 237 720.2 0 237 60 1110 720.2 0 1110 61 2224 720.2 0 2224 62 1494 720.2 0 1494 63 156 720.2 0 156 64 104 720.2 0 104 65 159 720.2 0 159 66 150 720.2 0 150 67 229 720.2 0 229 68 372 720.2 0 372 69 540 720.2 0 540 70 675 720.2 0 675 71 1475 720.2 0 1475 72 2393 720.2 0 2393 73 859 720.2 0 859 74 707 720.2 0 707 75 1263 720.2 0 1263 76 493 720.2 0 493 77 457 720.2 0 457 78 946 720.2 0 946 79 968 720.2 0 968 80 1344 720.2 0 1344 81 1270 720.2 0 1270 82 1821 720.2 0 1821 83 2354 720.2 0 2354 84 3288 720.2 0 3288 85 68445 720.2 0 68445 86 100473 720.2 0 100473 87 98361 720.2 0 98361 88 473 720.2 0 473 89 67 720.2 0 67 90 88 720.2 0 88 91 53 720.2 0 53 92 51 720.2 0 51 93 73 720.2 0 73 94 50 720.2 0 50 95 50 720.2 0 50 96 53 720.2 0 53 97 59 720.2 0 59 98 52 720.2 0 52 99 50 720.2 0 50 100 51 720.2 0 51 101 49 720.2 0 49 102 54 720.2 0 54 103 43 720.2 0 43 104 33 720.2 0 33 105 56 720.2 0 56 106 61 720.2 0 61 107 71 720.2 0 71 108 57 720.2 0 57 109 65 720.2 0 65 110 61 720.2 0 61 111 64 720.2 0 64 112 40 720.2 0 40 113 62 720.2 0 62 114 62 720.2 0 62 115 46 720.2 0 46 116 52 720.2 0 52 117 50 720.2 0 50 118 42 720.2 0 42 119 55 720.2 0 55 120 71 720.2 0 71 121 92 720.2 0 92 122 130 720.2 0 130 123 209 720.2 0 209 124 211 720.2 0 211 125 70 720.2 0 70 126 85 720.2 0 85 127 71 720.2 0 71 128 112 720.2 0 112 129 171 720.2 0 171 130 419 720.2 0 419 131 1090 720.2 0 1090 132 322 720.2 0 322 133 147 720.2 0 147 134 168 720.2 0 168 135 144 720.2 0 144 136 196 720.2 0 196 137 103 720.2 0 103 138 96 720.2 0 96 139 122 720.2 0 122 140 105 720.2 0 105 141 106 720.2 0 106 142 127 720.2 0 127 143 133 720.2 0 133 144 173 720.2 0 173 145 160 720.2 0 160 146 154 720.2 0 154 147 130 720.2 0 130 148 201 720.2 0 201 149 543 720.2 0 543 150 621 720.2 0 621 151 908 720.2 0 908 152 1571 720.2 0 1571 153 5969 720.2 0 5969 154 3210 720.2 0 3210 155 1894 720.2 0 1894 156 369 720.2 0 369 157 113 720.2 0 113 158 132 720.2 0 132 159 145 720.2 0 145 160 193 720.2 0 193 161 337 720.2 0 337 162 623 720.2 0 623 163 206 720.2 0 206 164 321 720.2 0 321 165 438 720.2 0 438 166 219 720.2 0 219 167 272 720.2 0 272 168 442 720.2 0 442 169 394 720.2 0 394 170 591 720.2 0 591 171 619 720.2 0 619 172 852 720.2 0 852 173 1432 720.2 0 1432 174 2000 720.2 0 2000 175 28806 720.2 0 28806 176 55777 720.2 0 55777 177 27608 720.2 0 27608 178 190 720.2 0 190 179 76 720.2 0 76 180 76 720.2 0 76 181 174 720.2 0 174 182 112 720.2 0 112 183 237 720.2 0 237 184 113 720.2 0 113 185 81 720.2 0 81 186 141 720.2 0 141 187 79 720.2 0 79 188 60 720.2 0 60 189 77 720.2 0 77 190 129 720.2 0 129 191 127 720.2 0 127 192 64 720.2 0 64 193 71 720.2 0 71 194 95 720.2 0 95 195 89 720.2 0 89 196 143 720.2 0 143 197 94 720.2 0 94 198 84 720.2 0 84 199 73 720.2 0 73 200 98 720.2 0 98 201 119 720.2 0 119 202 85 720.2 0 85 203 88 720.2 0 88 204 128 720.2 0 128 205 132 720.2 0 132 206 237 720.2 0 237 207 148 720.2 0 148 208 227 720.2 0 227 209 423 720.2 0 423 210 459 720.2 0 459 211 581 720.2 0 581 212 790 720.2 0 790 213 10049 720.2 0 10049 214 13186 720.2 0 13186 215 33101 720.2 0 33101 216 205 720.2 0 205 217 127 720.2 0 127 218 188 720.2 0 188 219 121 720.2 0 121 220 173 720.2 0 173 221 107 720.2 0 107 222 175 720.2 0 175 223 71 720.2 0 71 224 64 720.2 0 64 225 79 720.2 0 79 226 78 720.2 0 78 227 133 720.2 0 133 228 203 720.2 0 203 229 283 720.2 0 283 230 206 720.2 0 206 231 582 720.2 0 582 232 628 720.2 0 628 233 734 720.2 0 734 234 116 720.2 0 116 235 125 720.2 0 125 236 63 720.2 0 63 237 93 720.2 0 93 238 96 720.2 0 96 239 86 720.2 0 86 240 119 720.2 0 119 241 77 720.2 0 77 242 76 720.2 0 76 243 64 720.2 0 64 244 127 720.2 0 127 245 97 720.2 0 97 246 120 720.2 0 120 247 154 720.2 0 154 248 203 720.2 0 203 249 287 720.2 0 287 250 282 720.2 0 282 251 260 720.2 0 260 252 129 720.2 0 129 253 607 720.2 0 607 254 1326 720.2 0 1326 255 1049 720.2 0 1049 256 461 720.2 0 461 257 504 720.2 0 504 258 330 720.2 0 330 259 262 720.2 0 262 260 59 720.2 0 59 261 127 720.2 0 127 262 89 720.2 0 89 263 61 720.2 0 61 264 85 720.2 0 85 265 68 720.2 0 68 266 101 720.2 0 101 267 97 720.2 0 97 268 95 720.2 0 95 269 112 720.2 0 112 270 134 720.2 0 134 271 132 720.2 0 132 272 108 720.2 0 108 273 85 720.2 0 85 274 117 720.2 0 117 275 138 720.2 0 138 276 140 720.2 0 140 277 817 720.2 0 817 278 1128 720.2 0 1128 279 1209 720.2 0 1209 280 176 720.2 0 176 281 148 720.2 0 148 282 168 720.2 0 168 283 108 720.2 0 108 284 131 720.2 0 131 285 660 720.2 0 660 286 869 720.2 0 869 287 717 720.2 0 717 288 272 720.2 0 272 289 276 720.2 0 276 290 245 720.2 0 245 291 121 720.2 0 121 292 171 720.2 0 171 293 294 720.2 0 294 294 280 720.2 0 280 295 372 720.2 0 372 296 306 720.2 0 306 297 644 720.2 0 644 298 647 720.2 0 647 299 778 720.2 0 778 300 419 720.2 0 419 301 292 720.2 0 292 Finished in 29.51 s (63 us/read; 0.95 M reads/minute). === Summary === Total reads processed: 468,511 Reads with adapters: 10,177 (2.2%) Reads that were too short: 333 (0.1%) Reads written (passing filters): 9,844 (2.1%) Total basepairs processed: 90,548,868 bp Total written (filtered): 2,655,872 bp (2.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10177 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 3 19 53 0.0 3 0 39 7 4 3 20 9362 0.0 4 8883 381 70 19 9 21 16 0.0 4 2 11 1 0 2 22 6 0.0 4 2 0 1 0 3 23 20 0.0 4 1 1 0 15 3 30 2 0.0 4 0 0 0 1 1 34 1 0.0 4 0 0 0 1 40 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 42 1 0.0 4 1 45 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 49 283 0.0 4 0 0 0 0 283 50 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 57 6 0.0 4 0 0 0 0 6 59 2 0.0 4 1 0 0 0 1 66 2 0.0 4 0 0 0 0 2 67 7 0.0 4 0 0 0 0 7 68 1 0.0 4 0 0 0 0 1 74 2 0.0 4 0 0 0 0 2 75 1 0.0 4 0 0 0 0 1 76 11 0.0 4 0 0 0 0 11 77 5 0.0 4 0 0 0 0 5 78 3 0.0 4 0 0 0 2 1 79 2 0.0 4 0 0 0 0 2 85 1 0.0 4 0 0 1 87 1 0.0 4 0 0 0 0 1 89 3 0.0 4 0 0 0 0 3 90 4 0.0 4 0 0 0 0 4 91 15 0.0 4 0 0 0 0 15 92 1 0.0 4 0 0 0 0 1 100 7 0.0 4 0 0 0 0 7 104 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 1 1 114 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 124 2 0.0 4 0 0 0 0 2 125 1 0.0 4 0 0 0 0 1 127 23 0.0 4 0 0 0 0 23 128 35 0.0 4 0 0 0 0 35 129 11 0.0 4 1 0 0 0 10 130 2 0.0 4 0 0 0 0 2 131 1 0.0 4 0 0 0 0 1 135 2 0.0 4 0 0 0 0 2 136 8 0.0 4 0 0 0 0 8 137 5 0.0 4 0 0 0 0 5 138 2 0.0 4 0 0 0 0 2 139 3 0.0 4 0 0 0 0 3 146 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 150 88 0.0 4 0 0 0 1 87 151 55 0.0 4 0 0 0 4 51 152 6 0.0 4 0 0 0 1 5 153 1 0.0 4 0 0 0 0 1 155 2 0.0 4 0 0 0 0 2 156 3 0.0 4 0 0 0 0 3 157 2 0.0 4 0 0 0 0 2 158 3 0.0 4 0 0 0 0 3 160 2 0.0 4 0 0 0 0 2 162 1 0.0 4 0 0 0 0 1 167 2 0.0 4 0 0 0 0 2 171 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 181 2 0.0 4 0 0 0 0 2 182 7 0.0 4 0 0 0 0 7 183 1 0.0 4 0 0 0 0 1 184 6 0.0 4 0 0 0 0 6 185 6 0.0 4 0 0 0 0 6 186 4 0.0 4 0 0 0 1 3 187 7 0.0 4 0 0 0 0 7 188 3 0.0 4 0 0 0 0 3 189 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 191 2 0.0 4 0 0 0 0 2 196 1 0.0 4 0 0 0 0 1 197 2 0.0 4 0 0 0 0 2 199 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 207 2 0.0 4 0 0 0 0 2 208 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 215 4 0.0 4 0 0 0 0 4 216 6 0.0 4 0 0 0 1 5 217 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 240 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 279 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.56 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 9,844 Reads with adapters: 880 (8.9%) Reads written (passing filters): 9,844 (100.0%) Total basepairs processed: 2,655,872 bp Total written (filtered): 2,644,985 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 880 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.4% C: 7.4% G: 85.8% T: 2.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 39 153.8 0 39 4 8 38.5 0 8 5 1 9.6 0 1 9 1 0.0 0 0 1 10 10 0.0 1 0 10 11 684 0.0 1 16 668 12 89 0.0 1 5 84 13 31 0.0 1 12 19 14 4 0.0 1 3 1 15 2 0.0 1 0 2 21 1 0.0 1 1 152 8 0.0 1 4 4 153 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.