This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GACGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 145.48 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 512,045 (17.4%) Reads that were too short: 99,149 (3.4%) Reads written (passing filters): 412,896 (14.0%) Total basepairs processed: 887,907,860 bp Total written (filtered): 78,442,250 bp (8.8%) === Adapter 1 === Sequence: GACGGC; Type: regular 5'; Length: 6; Trimmed: 512045 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 969 720.2 0 969 7 11761 720.2 0 11761 8 130 720.2 0 130 9 9561 720.2 0 9561 10 71 720.2 0 71 11 57 720.2 0 57 12 46 720.2 0 46 13 43 720.2 0 43 14 47 720.2 0 47 15 58 720.2 0 58 16 76 720.2 0 76 17 75 720.2 0 75 18 55 720.2 0 55 19 83 720.2 0 83 20 79 720.2 0 79 21 73 720.2 0 73 22 70 720.2 0 70 23 54 720.2 0 54 24 61 720.2 0 61 25 61 720.2 0 61 26 54 720.2 0 54 27 68 720.2 0 68 28 79 720.2 0 79 29 51 720.2 0 51 30 87 720.2 0 87 31 56 720.2 0 56 32 69 720.2 0 69 33 82 720.2 0 82 34 85 720.2 0 85 35 93 720.2 0 93 36 116 720.2 0 116 37 67 720.2 0 67 38 71 720.2 0 71 39 46 720.2 0 46 40 54 720.2 0 54 41 114 720.2 0 114 42 137 720.2 0 137 43 133 720.2 0 133 44 80 720.2 0 80 45 114 720.2 0 114 46 108 720.2 0 108 47 150 720.2 0 150 48 69 720.2 0 69 49 153 720.2 0 153 50 424 720.2 0 424 51 198 720.2 0 198 52 85 720.2 0 85 53 108 720.2 0 108 54 94 720.2 0 94 55 184 720.2 0 184 56 80 720.2 0 80 57 96 720.2 0 96 58 122 720.2 0 122 59 135 720.2 0 135 60 363 720.2 0 363 61 952 720.2 0 952 62 532 720.2 0 532 63 146 720.2 0 146 64 98 720.2 0 98 65 155 720.2 0 155 66 142 720.2 0 142 67 213 720.2 0 213 68 305 720.2 0 305 69 467 720.2 0 467 70 584 720.2 0 584 71 1219 720.2 0 1219 72 1995 720.2 0 1995 73 657 720.2 0 657 74 621 720.2 0 621 75 1005 720.2 0 1005 76 401 720.2 0 401 77 425 720.2 0 425 78 861 720.2 0 861 79 777 720.2 0 777 80 1025 720.2 0 1025 81 957 720.2 0 957 82 1305 720.2 0 1305 83 1635 720.2 0 1635 84 2152 720.2 0 2152 85 58206 720.2 0 58206 86 82957 720.2 0 82957 87 79964 720.2 0 79964 88 351 720.2 0 351 89 60 720.2 0 60 90 97 720.2 0 97 91 76 720.2 0 76 92 64 720.2 0 64 93 66 720.2 0 66 94 71 720.2 0 71 95 68 720.2 0 68 96 75 720.2 0 75 97 66 720.2 0 66 98 82 720.2 0 82 99 62 720.2 0 62 100 82 720.2 0 82 101 61 720.2 0 61 102 72 720.2 0 72 103 68 720.2 0 68 104 50 720.2 0 50 105 64 720.2 0 64 106 87 720.2 0 87 107 90 720.2 0 90 108 67 720.2 0 67 109 83 720.2 0 83 110 78 720.2 0 78 111 71 720.2 0 71 112 51 720.2 0 51 113 59 720.2 0 59 114 64 720.2 0 64 115 79 720.2 0 79 116 57 720.2 0 57 117 56 720.2 0 56 118 66 720.2 0 66 119 49 720.2 0 49 120 54 720.2 0 54 121 70 720.2 0 70 122 75 720.2 0 75 123 97 720.2 0 97 124 118 720.2 0 118 125 76 720.2 0 76 126 75 720.2 0 75 127 46 720.2 0 46 128 87 720.2 0 87 129 122 720.2 0 122 130 158 720.2 0 158 131 440 720.2 0 440 132 189 720.2 0 189 133 88 720.2 0 88 134 102 720.2 0 102 135 95 720.2 0 95 136 100 720.2 0 100 137 85 720.2 0 85 138 92 720.2 0 92 139 107 720.2 0 107 140 92 720.2 0 92 141 107 720.2 0 107 142 106 720.2 0 106 143 130 720.2 0 130 144 156 720.2 0 156 145 152 720.2 0 152 146 117 720.2 0 117 147 91 720.2 0 91 148 141 720.2 0 141 149 431 720.2 0 431 150 390 720.2 0 390 151 817 720.2 0 817 152 1142 720.2 0 1142 153 4780 720.2 0 4780 154 2445 720.2 0 2445 155 1410 720.2 0 1410 156 210 720.2 0 210 157 107 720.2 0 107 158 118 720.2 0 118 159 168 720.2 0 168 160 225 720.2 0 225 161 347 720.2 0 347 162 706 720.2 0 706 163 233 720.2 0 233 164 327 720.2 0 327 165 493 720.2 0 493 166 239 720.2 0 239 167 210 720.2 0 210 168 481 720.2 0 481 169 392 720.2 0 392 170 509 720.2 0 509 171 602 720.2 0 602 172 774 720.2 0 774 173 1329 720.2 0 1329 174 1544 720.2 0 1544 175 28478 720.2 0 28478 176 59551 720.2 0 59551 177 29203 720.2 0 29203 178 171 720.2 0 171 179 80 720.2 0 80 180 87 720.2 0 87 181 101 720.2 0 101 182 71 720.2 0 71 183 137 720.2 0 137 184 87 720.2 0 87 185 71 720.2 0 71 186 137 720.2 0 137 187 58 720.2 0 58 188 60 720.2 0 60 189 57 720.2 0 57 190 77 720.2 0 77 191 89 720.2 0 89 192 57 720.2 0 57 193 57 720.2 0 57 194 58 720.2 0 58 195 91 720.2 0 91 196 169 720.2 0 169 197 76 720.2 0 76 198 69 720.2 0 69 199 67 720.2 0 67 200 95 720.2 0 95 201 142 720.2 0 142 202 76 720.2 0 76 203 66 720.2 0 66 204 89 720.2 0 89 205 122 720.2 0 122 206 166 720.2 0 166 207 158 720.2 0 158 208 231 720.2 0 231 209 551 720.2 0 551 210 514 720.2 0 514 211 748 720.2 0 748 212 916 720.2 0 916 213 13233 720.2 0 13233 214 20711 720.2 0 20711 215 47525 720.2 0 47525 216 230 720.2 0 230 217 80 720.2 0 80 218 130 720.2 0 130 219 102 720.2 0 102 220 94 720.2 0 94 221 89 720.2 0 89 222 151 720.2 0 151 223 47 720.2 0 47 224 59 720.2 0 59 225 48 720.2 0 48 226 62 720.2 0 62 227 85 720.2 0 85 228 102 720.2 0 102 229 127 720.2 0 127 230 112 720.2 0 112 231 401 720.2 0 401 232 486 720.2 0 486 233 498 720.2 0 498 234 87 720.2 0 87 235 64 720.2 0 64 236 49 720.2 0 49 237 57 720.2 0 57 238 59 720.2 0 59 239 62 720.2 0 62 240 74 720.2 0 74 241 76 720.2 0 76 242 58 720.2 0 58 243 55 720.2 0 55 244 83 720.2 0 83 245 79 720.2 0 79 246 61 720.2 0 61 247 67 720.2 0 67 248 90 720.2 0 90 249 110 720.2 0 110 250 104 720.2 0 104 251 104 720.2 0 104 252 71 720.2 0 71 253 255 720.2 0 255 254 494 720.2 0 494 255 382 720.2 0 382 256 202 720.2 0 202 257 384 720.2 0 384 258 212 720.2 0 212 259 171 720.2 0 171 260 45 720.2 0 45 261 72 720.2 0 72 262 53 720.2 0 53 263 53 720.2 0 53 264 53 720.2 0 53 265 67 720.2 0 67 266 48 720.2 0 48 267 67 720.2 0 67 268 61 720.2 0 61 269 60 720.2 0 60 270 59 720.2 0 59 271 80 720.2 0 80 272 81 720.2 0 81 273 61 720.2 0 61 274 76 720.2 0 76 275 65 720.2 0 65 276 94 720.2 0 94 277 824 720.2 0 824 278 1196 720.2 0 1196 279 1252 720.2 0 1252 280 59 720.2 0 59 281 74 720.2 0 74 282 74 720.2 0 74 283 67 720.2 0 67 284 59 720.2 0 59 285 220 720.2 0 220 286 280 720.2 0 280 287 233 720.2 0 233 288 160 720.2 0 160 289 166 720.2 0 166 290 164 720.2 0 164 291 69 720.2 0 69 292 89 720.2 0 89 293 118 720.2 0 118 294 159 720.2 0 159 295 155 720.2 0 155 296 148 720.2 0 148 297 204 720.2 0 204 298 232 720.2 0 232 299 269 720.2 0 269 300 154 720.2 0 154 301 110 720.2 0 110 Finished in 23.03 s (56 us/read; 1.08 M reads/minute). === Summary === Total reads processed: 412,896 Reads with adapters: 13,283 (3.2%) Reads that were too short: 489 (0.1%) Reads written (passing filters): 12,794 (3.1%) Total basepairs processed: 78,442,250 bp Total written (filtered): 3,152,692 bp (4.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13283 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 0 3 16 48 0.0 3 0 46 1 1 17 10214 0.0 3 9382 216 11 605 18 26 0.0 3 2 23 0 1 19 681 0.0 3 660 14 5 2 20 118 0.0 3 1 2 2 113 21 2 0.0 3 0 0 2 22 2 0.0 3 0 0 0 2 27 1 0.0 3 1 37 146 0.0 3 0 0 0 146 38 19 0.0 3 0 0 0 19 39 1 0.0 3 0 0 0 1 41 6 0.0 3 0 0 0 6 43 3 0.0 3 0 0 0 3 44 1 0.0 3 0 0 0 1 45 9 0.0 3 0 0 0 9 51 3 0.0 3 0 0 0 3 53 5 0.0 3 0 0 0 5 55 1 0.0 3 0 0 0 1 56 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 65 13 0.0 3 0 0 0 13 68 5 0.0 3 0 0 0 5 70 2 0.0 3 0 0 0 2 72 1 0.0 3 1 77 13 0.0 3 0 0 1 12 80 2 0.0 3 0 0 0 2 81 3 0.0 3 0 0 1 2 82 4 0.0 3 0 0 0 4 84 1 0.0 3 0 0 0 1 85 3 0.0 3 0 0 0 3 86 537 0.0 3 0 0 3 534 87 253 0.0 3 0 0 3 250 88 114 0.0 3 0 0 1 113 89 203 0.0 3 0 0 3 200 90 64 0.0 3 0 0 1 63 91 17 0.0 3 0 0 0 17 92 24 0.0 3 0 0 0 24 93 4 0.0 3 0 0 0 4 94 5 0.0 3 0 0 1 4 95 3 0.0 3 0 0 0 3 96 1 0.0 3 0 0 0 1 97 5 0.0 3 0 0 0 5 98 6 0.0 3 0 0 0 6 99 7 0.0 3 0 0 0 7 100 12 0.0 3 0 0 0 12 101 16 0.0 3 0 0 0 16 102 6 0.0 3 0 0 0 6 103 2 0.0 3 0 0 0 2 104 3 0.0 3 0 0 0 3 105 1 0.0 3 0 0 0 1 106 18 0.0 3 0 0 0 18 107 89 0.0 3 0 0 0 89 110 18 0.0 3 0 0 0 18 112 16 0.0 3 0 0 0 16 113 9 0.0 3 0 0 0 9 114 4 0.0 3 0 0 0 4 115 2 0.0 3 0 0 0 2 118 4 0.0 3 0 0 0 4 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 131 30 0.0 3 0 0 0 30 132 2 0.0 3 0 0 0 2 134 4 0.0 3 0 0 0 4 135 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 138 4 0.0 3 0 0 0 4 140 1 0.0 3 0 0 0 1 141 3 0.0 3 0 0 0 3 143 13 0.0 3 0 0 0 13 146 4 0.0 3 0 0 1 3 147 3 0.0 3 0 0 0 3 152 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 20 0.0 3 0 0 0 20 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 167 6 0.0 3 0 0 0 6 176 2 0.0 3 0 0 0 2 177 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 189 2 0.0 3 0 0 1 1 191 1 0.0 3 0 0 0 1 193 16 0.0 3 0 0 0 16 196 2 0.0 3 0 0 0 2 198 70 0.0 3 0 0 0 70 199 38 0.0 3 0 0 0 38 200 35 0.0 3 0 0 0 35 201 55 0.0 3 0 0 3 52 202 58 0.0 3 0 0 0 58 203 15 0.0 3 0 0 2 13 204 8 0.0 3 0 0 0 8 205 3 0.0 3 0 0 0 3 206 8 0.0 3 0 0 0 8 207 4 0.0 3 0 0 0 4 209 2 0.0 3 0 0 0 2 210 1 0.0 3 0 0 0 1 211 2 0.0 3 0 0 0 2 212 3 0.0 3 0 0 0 3 213 9 0.0 3 0 0 0 9 214 21 0.0 3 0 0 1 20 215 2 0.0 3 0 0 0 2 216 20 0.0 3 0 0 0 20 217 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 226 3 0.0 3 0 0 0 3 233 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.72 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 12,794 Reads with adapters: 335 (2.6%) Reads written (passing filters): 12,794 (100.0%) Total basepairs processed: 3,152,692 bp Total written (filtered): 3,151,667 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 335 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 67.5% C: 6.0% G: 26.0% T: 0.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 323 199.9 0 323 4 11 50.0 0 11 12 1 0.0 1 0 1