This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 139.24 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 50,408 (1.7%) Reads that were too short: 8,118 (0.3%) Reads written (passing filters): 42,290 (1.4%) Total basepairs processed: 887,907,860 bp Total written (filtered): 11,264,927 bp (1.3%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 50408 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 225 720.2 0 225 7 389 720.2 0 389 8 18876 720.2 0 18876 9 12967 720.2 0 12967 10 112 720.2 0 112 11 2 720.2 0 2 12 1 720.2 0 1 14 5 720.2 0 5 15 2 720.2 0 2 16 1 720.2 0 1 17 2 720.2 0 2 18 4 720.2 0 4 19 4 720.2 0 4 20 2 720.2 0 2 21 13 720.2 0 13 22 4 720.2 0 4 23 10 720.2 0 10 24 3 720.2 0 3 25 9 720.2 0 9 26 1 720.2 0 1 28 3 720.2 0 3 29 7 720.2 0 7 30 8 720.2 0 8 31 8 720.2 0 8 32 5 720.2 0 5 33 26 720.2 0 26 34 23 720.2 0 23 35 21 720.2 0 21 36 40 720.2 0 40 37 12 720.2 0 12 38 48 720.2 0 48 39 5 720.2 0 5 40 50 720.2 0 50 41 4 720.2 0 4 42 2 720.2 0 2 43 3 720.2 0 3 44 5 720.2 0 5 45 15 720.2 0 15 46 7 720.2 0 7 47 1 720.2 0 1 48 5 720.2 0 5 49 4 720.2 0 4 50 6 720.2 0 6 51 10 720.2 0 10 52 12 720.2 0 12 53 7 720.2 0 7 54 6 720.2 0 6 55 11 720.2 0 11 56 2 720.2 0 2 57 6 720.2 0 6 58 77 720.2 0 77 59 60 720.2 0 60 60 45 720.2 0 45 61 4 720.2 0 4 62 17 720.2 0 17 63 12 720.2 0 12 64 7 720.2 0 7 65 4 720.2 0 4 66 7 720.2 0 7 67 9 720.2 0 9 68 12 720.2 0 12 69 14 720.2 0 14 70 50 720.2 0 50 71 116 720.2 0 116 72 13 720.2 0 13 73 30 720.2 0 30 74 15 720.2 0 15 75 17 720.2 0 17 76 89 720.2 0 89 77 526 720.2 0 526 78 239 720.2 0 239 79 16 720.2 0 16 80 233 720.2 0 233 81 144 720.2 0 144 82 232 720.2 0 232 83 16 720.2 0 16 84 6 720.2 0 6 85 11 720.2 0 11 86 7 720.2 0 7 87 23 720.2 0 23 88 12 720.2 0 12 89 24 720.2 0 24 90 22 720.2 0 22 91 9 720.2 0 9 92 4 720.2 0 4 93 13 720.2 0 13 94 13 720.2 0 13 95 21 720.2 0 21 96 15 720.2 0 15 97 10 720.2 0 10 98 15 720.2 0 15 99 37 720.2 0 37 100 391 720.2 0 391 101 598 720.2 0 598 102 1025 720.2 0 1025 103 27 720.2 0 27 104 8 720.2 0 8 105 19 720.2 0 19 106 7 720.2 0 7 107 21 720.2 0 21 108 15 720.2 0 15 109 55 720.2 0 55 110 579 720.2 0 579 111 305 720.2 0 305 112 232 720.2 0 232 113 15 720.2 0 15 114 4 720.2 0 4 115 8 720.2 0 8 116 66 720.2 0 66 117 12 720.2 0 12 118 8 720.2 0 8 119 7 720.2 0 7 120 2 720.2 0 2 121 4 720.2 0 4 122 10 720.2 0 10 123 20 720.2 0 20 124 13 720.2 0 13 125 9 720.2 0 9 126 15 720.2 0 15 127 95 720.2 0 95 128 8 720.2 0 8 129 10 720.2 0 10 130 14 720.2 0 14 131 20 720.2 0 20 132 29 720.2 0 29 133 24 720.2 0 24 134 9 720.2 0 9 135 5 720.2 0 5 136 9 720.2 0 9 137 39 720.2 0 39 138 13 720.2 0 13 139 9 720.2 0 9 140 29 720.2 0 29 141 15 720.2 0 15 142 18 720.2 0 18 143 22 720.2 0 22 144 20 720.2 0 20 145 20 720.2 0 20 146 17 720.2 0 17 147 31 720.2 0 31 148 47 720.2 0 47 149 28 720.2 0 28 150 28 720.2 0 28 151 51 720.2 0 51 152 414 720.2 0 414 153 23 720.2 0 23 154 8 720.2 0 8 155 8 720.2 0 8 156 11 720.2 0 11 157 34 720.2 0 34 158 46 720.2 0 46 159 23 720.2 0 23 160 23 720.2 0 23 161 11 720.2 0 11 162 25 720.2 0 25 163 11 720.2 0 11 164 6 720.2 0 6 165 5 720.2 0 5 166 12 720.2 0 12 167 13 720.2 0 13 168 81 720.2 0 81 169 18 720.2 0 18 170 41 720.2 0 41 171 6 720.2 0 6 172 5 720.2 0 5 173 6 720.2 0 6 174 10 720.2 0 10 175 7 720.2 0 7 176 6 720.2 0 6 177 14 720.2 0 14 178 28 720.2 0 28 179 11 720.2 0 11 180 9 720.2 0 9 181 13 720.2 0 13 182 26 720.2 0 26 183 38 720.2 0 38 184 37 720.2 0 37 185 32 720.2 0 32 186 46 720.2 0 46 187 23 720.2 0 23 188 14 720.2 0 14 189 22 720.2 0 22 190 20 720.2 0 20 191 21 720.2 0 21 192 17 720.2 0 17 193 17 720.2 0 17 194 32 720.2 0 32 195 24 720.2 0 24 196 31 720.2 0 31 197 56 720.2 0 56 198 109 720.2 0 109 199 564 720.2 0 564 200 722 720.2 0 722 201 186 720.2 0 186 202 141 720.2 0 141 203 226 720.2 0 226 204 311 720.2 0 311 205 67 720.2 0 67 206 38 720.2 0 38 207 28 720.2 0 28 208 28 720.2 0 28 209 40 720.2 0 40 210 26 720.2 0 26 211 16 720.2 0 16 212 19 720.2 0 19 213 26 720.2 0 26 214 27 720.2 0 27 215 25 720.2 0 25 216 35 720.2 0 35 217 122 720.2 0 122 218 261 720.2 0 261 219 269 720.2 0 269 220 57 720.2 0 57 221 46 720.2 0 46 222 462 720.2 0 462 223 817 720.2 0 817 224 648 720.2 0 648 225 74 720.2 0 74 226 45 720.2 0 45 227 26 720.2 0 26 228 29 720.2 0 29 229 41 720.2 0 41 230 19 720.2 0 19 231 24 720.2 0 24 232 15 720.2 0 15 233 15 720.2 0 15 234 20 720.2 0 20 235 36 720.2 0 36 236 28 720.2 0 28 237 28 720.2 0 28 238 26 720.2 0 26 239 56 720.2 0 56 240 48 720.2 0 48 241 64 720.2 0 64 242 119 720.2 0 119 243 50 720.2 0 50 244 27 720.2 0 27 245 12 720.2 0 12 246 26 720.2 0 26 247 28 720.2 0 28 248 24 720.2 0 24 249 22 720.2 0 22 250 28 720.2 0 28 251 27 720.2 0 27 252 38 720.2 0 38 253 27 720.2 0 27 254 33 720.2 0 33 255 68 720.2 0 68 256 84 720.2 0 84 257 163 720.2 0 163 258 112 720.2 0 112 259 74 720.2 0 74 260 55 720.2 0 55 261 35 720.2 0 35 262 50 720.2 0 50 263 44 720.2 0 44 264 87 720.2 0 87 265 57 720.2 0 57 266 27 720.2 0 27 267 37 720.2 0 37 268 24 720.2 0 24 269 28 720.2 0 28 270 30 720.2 0 30 271 37 720.2 0 37 272 48 720.2 0 48 273 54 720.2 0 54 274 30 720.2 0 30 275 37 720.2 0 37 276 50 720.2 0 50 277 81 720.2 0 81 278 128 720.2 0 128 279 76 720.2 0 76 280 45 720.2 0 45 281 43 720.2 0 43 282 53 720.2 0 53 283 47 720.2 0 47 284 34 720.2 0 34 285 71 720.2 0 71 286 33 720.2 0 33 287 31 720.2 0 31 288 56 720.2 0 56 289 53 720.2 0 53 290 66 720.2 0 66 291 71 720.2 0 71 292 400 720.2 0 400 293 187 720.2 0 187 294 177 720.2 0 177 295 63 720.2 0 63 296 53 720.2 0 53 297 59 720.2 0 59 298 59 720.2 0 59 299 75 720.2 0 75 300 80 720.2 0 80 301 56 720.2 0 56 Finished in 3.30 s (78 us/read; 0.77 M reads/minute). === Summary === Total reads processed: 42,290 Reads with adapters: 13,407 (31.7%) Reads that were too short: 26 (0.1%) Reads written (passing filters): 13,381 (31.6%) Total basepairs processed: 11,264,927 bp Total written (filtered): 3,628,938 bp (32.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 13407 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 7 0.0 3 0 0 6 0 1 19 51 0.0 3 0 40 6 3 2 20 13153 0.0 4 12579 445 99 23 7 21 26 0.0 4 2 22 2 22 4 0.0 4 1 2 1 23 29 0.0 4 0 1 0 22 6 24 2 0.0 4 1 0 0 0 1 27 1 0.0 4 0 0 1 31 1 0.0 4 0 0 1 41 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 76 4 0.0 4 0 0 0 0 4 78 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 13 0.0 4 0 0 0 0 13 127 83 0.0 4 0 0 0 0 83 128 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 1 2 229 4 0.0 4 0 0 0 1 3 230 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 0 2 282 2 0.0 4 0 0 0 0 2 285 1 0.0 4 0 0 0 0 1 288 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.83 s (62 us/read; 0.97 M reads/minute). === Summary === Total reads processed: 13,381 Reads with adapters: 3,507 (26.2%) Reads written (passing filters): 13,381 (100.0%) Total basepairs processed: 3,628,938 bp Total written (filtered): 3,594,439 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3507 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.1% C: 4.8% G: 92.5% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 209.1 0 3 4 2 52.3 0 2 6 1 3.3 0 1 9 1710 0.1 0 71 1639 10 1543 0.0 1 67 1476 11 230 0.0 1 4 226 12 8 0.0 1 0 8 13 1 0.0 1 0 1 18 4 0.0 1 2 2 150 1 0.0 1 0 1 169 1 0.0 1 1 208 2 0.0 1 2 210 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.