This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 144.20 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 255,698 (8.7%) Reads that were too short: 4,003 (0.1%) Reads written (passing filters): 251,695 (8.5%) Total basepairs processed: 887,907,860 bp Total written (filtered): 41,974,237 bp (4.7%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 255698 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 211 720.2 0 211 7 8768 720.2 0 8768 8 7493 720.2 0 7493 9 8 720.2 0 8 10 16 720.2 0 16 11 39 720.2 0 39 12 169 720.2 0 169 13 200 720.2 0 200 14 13 720.2 0 13 15 6 720.2 0 6 16 2 720.2 0 2 17 5 720.2 0 5 18 8 720.2 0 8 19 10 720.2 0 10 20 15 720.2 0 15 21 17 720.2 0 17 22 13 720.2 0 13 23 11 720.2 0 11 24 15 720.2 0 15 25 22 720.2 0 22 26 18 720.2 0 18 27 56 720.2 0 56 28 14 720.2 0 14 29 5 720.2 0 5 30 9 720.2 0 9 31 16 720.2 0 16 32 25 720.2 0 25 33 16 720.2 0 16 34 14 720.2 0 14 35 14 720.2 0 14 36 6 720.2 0 6 37 16 720.2 0 16 38 44 720.2 0 44 39 27 720.2 0 27 40 29 720.2 0 29 41 12 720.2 0 12 42 13 720.2 0 13 43 32 720.2 0 32 44 22 720.2 0 22 45 11 720.2 0 11 46 9 720.2 0 9 47 10 720.2 0 10 48 11 720.2 0 11 49 9 720.2 0 9 50 3 720.2 0 3 51 40 720.2 0 40 52 10 720.2 0 10 53 12 720.2 0 12 54 18 720.2 0 18 55 14 720.2 0 14 56 5 720.2 0 5 57 9 720.2 0 9 58 8 720.2 0 8 59 19 720.2 0 19 60 21 720.2 0 21 61 9 720.2 0 9 62 4 720.2 0 4 63 7 720.2 0 7 64 11 720.2 0 11 65 11 720.2 0 11 66 19 720.2 0 19 67 10 720.2 0 10 68 7 720.2 0 7 69 69 720.2 0 69 70 33 720.2 0 33 71 34 720.2 0 34 72 19 720.2 0 19 73 25 720.2 0 25 74 116 720.2 0 116 75 22 720.2 0 22 76 18 720.2 0 18 77 10 720.2 0 10 78 13 720.2 0 13 79 12 720.2 0 12 80 18 720.2 0 18 81 9 720.2 0 9 82 33 720.2 0 33 83 13 720.2 0 13 84 35 720.2 0 35 85 20 720.2 0 20 86 13 720.2 0 13 87 10 720.2 0 10 88 8 720.2 0 8 89 18 720.2 0 18 90 15 720.2 0 15 91 27 720.2 0 27 92 40 720.2 0 40 93 22 720.2 0 22 94 22 720.2 0 22 95 271 720.2 0 271 96 23 720.2 0 23 97 44 720.2 0 44 98 58 720.2 0 58 99 39 720.2 0 39 100 13 720.2 0 13 101 21 720.2 0 21 102 94 720.2 0 94 103 34 720.2 0 34 104 97 720.2 0 97 105 122 720.2 0 122 106 74 720.2 0 74 107 63 720.2 0 63 108 84 720.2 0 84 109 50 720.2 0 50 110 25 720.2 0 25 111 50 720.2 0 50 112 106 720.2 0 106 113 71 720.2 0 71 114 67 720.2 0 67 115 369 720.2 0 369 116 34 720.2 0 34 117 22 720.2 0 22 118 29 720.2 0 29 119 16 720.2 0 16 120 35 720.2 0 35 121 35 720.2 0 35 122 200 720.2 0 200 123 108 720.2 0 108 124 328 720.2 0 328 125 489 720.2 0 489 126 109 720.2 0 109 127 222 720.2 0 222 128 149 720.2 0 149 129 260 720.2 0 260 130 505 720.2 0 505 131 172 720.2 0 172 132 153 720.2 0 153 133 74 720.2 0 74 134 128 720.2 0 128 135 321 720.2 0 321 136 413 720.2 0 413 137 650 720.2 0 650 138 1003 720.2 0 1003 139 1177 720.2 0 1177 140 2759 720.2 0 2759 141 4401 720.2 0 4401 142 84677 720.2 0 84677 143 36399 720.2 0 36399 144 78687 720.2 0 78687 145 344 720.2 0 344 146 51 720.2 0 51 147 54 720.2 0 54 148 33 720.2 0 33 149 56 720.2 0 56 150 74 720.2 0 74 151 24 720.2 0 24 152 26 720.2 0 26 153 10 720.2 0 10 154 24 720.2 0 24 155 41 720.2 0 41 156 46 720.2 0 46 157 102 720.2 0 102 158 113 720.2 0 113 159 105 720.2 0 105 160 246 720.2 0 246 161 339 720.2 0 339 162 6151 720.2 0 6151 163 1851 720.2 0 1851 164 7316 720.2 0 7316 165 42 720.2 0 42 166 10 720.2 0 10 167 20 720.2 0 20 168 19 720.2 0 19 169 36 720.2 0 36 170 10 720.2 0 10 171 13 720.2 0 13 172 11 720.2 0 11 173 10 720.2 0 10 174 9 720.2 0 9 175 16 720.2 0 16 176 24 720.2 0 24 177 57 720.2 0 57 178 34 720.2 0 34 179 26 720.2 0 26 180 9 720.2 0 9 181 7 720.2 0 7 182 19 720.2 0 19 183 13 720.2 0 13 184 29 720.2 0 29 185 22 720.2 0 22 186 14 720.2 0 14 187 29 720.2 0 29 188 210 720.2 0 210 189 51 720.2 0 51 190 86 720.2 0 86 191 10 720.2 0 10 192 11 720.2 0 11 193 16 720.2 0 16 194 8 720.2 0 8 195 11 720.2 0 11 196 14 720.2 0 14 197 17 720.2 0 17 198 10 720.2 0 10 199 16 720.2 0 16 200 14 720.2 0 14 201 16 720.2 0 16 202 20 720.2 0 20 203 15 720.2 0 15 204 12 720.2 0 12 205 12 720.2 0 12 206 21 720.2 0 21 207 29 720.2 0 29 208 24 720.2 0 24 209 38 720.2 0 38 210 48 720.2 0 48 211 50 720.2 0 50 212 56 720.2 0 56 213 35 720.2 0 35 214 88 720.2 0 88 215 73 720.2 0 73 216 115 720.2 0 115 217 49 720.2 0 49 218 40 720.2 0 40 219 14 720.2 0 14 220 15 720.2 0 15 221 24 720.2 0 24 222 39 720.2 0 39 223 61 720.2 0 61 224 85 720.2 0 85 225 140 720.2 0 140 226 33 720.2 0 33 227 50 720.2 0 50 228 11 720.2 0 11 229 12 720.2 0 12 230 13 720.2 0 13 231 22 720.2 0 22 232 44 720.2 0 44 233 71 720.2 0 71 234 66 720.2 0 66 235 12 720.2 0 12 236 23 720.2 0 23 237 20 720.2 0 20 238 36 720.2 0 36 239 32 720.2 0 32 240 42 720.2 0 42 241 21 720.2 0 21 242 24 720.2 0 24 243 37 720.2 0 37 244 27 720.2 0 27 245 20 720.2 0 20 246 29 720.2 0 29 247 29 720.2 0 29 248 16 720.2 0 16 249 17 720.2 0 17 250 18 720.2 0 18 251 15 720.2 0 15 252 20 720.2 0 20 253 26 720.2 0 26 254 23 720.2 0 23 255 26 720.2 0 26 256 42 720.2 0 42 257 33 720.2 0 33 258 35 720.2 0 35 259 50 720.2 0 50 260 48 720.2 0 48 261 47 720.2 0 47 262 60 720.2 0 60 263 98 720.2 0 98 264 68 720.2 0 68 265 30 720.2 0 30 266 27 720.2 0 27 267 22 720.2 0 22 268 33 720.2 0 33 269 59 720.2 0 59 270 55 720.2 0 55 271 41 720.2 0 41 272 17 720.2 0 17 273 17 720.2 0 17 274 40 720.2 0 40 275 32 720.2 0 32 276 83 720.2 0 83 277 67 720.2 0 67 278 53 720.2 0 53 279 71 720.2 0 71 280 68 720.2 0 68 281 117 720.2 0 117 282 44 720.2 0 44 283 58 720.2 0 58 284 50 720.2 0 50 285 52 720.2 0 52 286 29 720.2 0 29 287 35 720.2 0 35 288 23 720.2 0 23 289 21 720.2 0 21 290 21 720.2 0 21 291 24 720.2 0 24 292 18 720.2 0 18 293 25 720.2 0 25 294 26 720.2 0 26 295 40 720.2 0 40 296 146 720.2 0 146 297 47 720.2 0 47 298 24 720.2 0 24 299 24 720.2 0 24 300 27 720.2 0 27 301 18 720.2 0 18 Finished in 14.71 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 251,695 Reads with adapters: 8,167 (3.2%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 8,158 (3.2%) Total basepairs processed: 41,974,237 bp Total written (filtered): 2,176,880 bp (5.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8167 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 2 0.0 3 0 0 0 1 1 19 45 0.0 3 0 30 7 7 1 20 7603 0.0 4 7256 279 54 12 2 21 20 0.0 4 4 13 1 2 22 1 0.0 4 0 0 0 1 23 21 0.0 4 1 1 0 12 7 27 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 0 1 36 7 0.0 4 0 0 0 0 7 37 13 0.0 4 0 0 0 0 13 38 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 123 16 0.0 4 0 0 0 0 16 126 12 0.0 4 0 0 0 0 12 127 410 0.0 4 0 0 0 0 410 128 2 0.0 4 0 0 0 0 2 130 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.49 s (60 us/read; 1.00 M reads/minute). === Summary === Total reads processed: 8,158 Reads with adapters: 1,577 (19.3%) Reads written (passing filters): 8,158 (100.0%) Total basepairs processed: 2,176,880 bp Total written (filtered): 2,154,431 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1577 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.4% C: 5.8% G: 90.1% T: 2.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 7 127.5 0 7 4 3 31.9 0 3 9 7 0.0 0 0 7 10 266 0.0 1 9 257 11 1259 0.0 1 25 1234 12 9 0.0 1 2 7 151 1 0.0 1 0 1 209 1 0.0 1 1 224 24 0.0 1 21 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.