This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 140.74 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 187,348 (6.4%) Reads that were too short: 23,573 (0.8%) Reads written (passing filters): 163,775 (5.6%) Total basepairs processed: 887,907,860 bp Total written (filtered): 45,139,001 bp (5.1%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 187348 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 681 720.2 0 681 7 8013 720.2 0 8013 8 446 720.2 0 446 9 6415 720.2 0 6415 10 964 720.2 0 964 11 1093 720.2 0 1093 12 11685 720.2 0 11685 13 64592 720.2 0 64592 14 51942 720.2 0 51942 15 132 720.2 0 132 16 42 720.2 0 42 17 39 720.2 0 39 18 25 720.2 0 25 19 29 720.2 0 29 20 23 720.2 0 23 21 32 720.2 0 32 22 52 720.2 0 52 23 37 720.2 0 37 24 45 720.2 0 45 25 31 720.2 0 31 26 56 720.2 0 56 27 67 720.2 0 67 28 70 720.2 0 70 29 45 720.2 0 45 30 43 720.2 0 43 31 38 720.2 0 38 32 29 720.2 0 29 33 31 720.2 0 31 34 47 720.2 0 47 35 36 720.2 0 36 36 30 720.2 0 30 37 26 720.2 0 26 38 30 720.2 0 30 39 31 720.2 0 31 40 64 720.2 0 64 41 38 720.2 0 38 42 28 720.2 0 28 43 43 720.2 0 43 44 76 720.2 0 76 45 159 720.2 0 159 46 38 720.2 0 38 47 29 720.2 0 29 48 23 720.2 0 23 49 51 720.2 0 51 50 32 720.2 0 32 51 27 720.2 0 27 52 25 720.2 0 25 53 50 720.2 0 50 54 32 720.2 0 32 55 35 720.2 0 35 56 34 720.2 0 34 57 44 720.2 0 44 58 40 720.2 0 40 59 29 720.2 0 29 60 21 720.2 0 21 61 39 720.2 0 39 62 30 720.2 0 30 63 41 720.2 0 41 64 33 720.2 0 33 65 47 720.2 0 47 66 46 720.2 0 46 67 54 720.2 0 54 68 27 720.2 0 27 69 42 720.2 0 42 70 43 720.2 0 43 71 31 720.2 0 31 72 31 720.2 0 31 73 50 720.2 0 50 74 41 720.2 0 41 75 25 720.2 0 25 76 41 720.2 0 41 77 34 720.2 0 34 78 43 720.2 0 43 79 42 720.2 0 42 80 41 720.2 0 41 81 32 720.2 0 32 82 29 720.2 0 29 83 41 720.2 0 41 84 42 720.2 0 42 85 33 720.2 0 33 86 36 720.2 0 36 87 37 720.2 0 37 88 24 720.2 0 24 89 39 720.2 0 39 90 48 720.2 0 48 91 70 720.2 0 70 92 82 720.2 0 82 93 53 720.2 0 53 94 67 720.2 0 67 95 55 720.2 0 55 96 28 720.2 0 28 97 38 720.2 0 38 98 49 720.2 0 49 99 54 720.2 0 54 100 51 720.2 0 51 101 48 720.2 0 48 102 44 720.2 0 44 103 40 720.2 0 40 104 163 720.2 0 163 105 256 720.2 0 256 106 326 720.2 0 326 107 56 720.2 0 56 108 105 720.2 0 105 109 64 720.2 0 64 110 105 720.2 0 105 111 77 720.2 0 77 112 108 720.2 0 108 113 76 720.2 0 76 114 90 720.2 0 90 115 75 720.2 0 75 116 79 720.2 0 79 117 58 720.2 0 58 118 48 720.2 0 48 119 51 720.2 0 51 120 63 720.2 0 63 121 50 720.2 0 50 122 58 720.2 0 58 123 61 720.2 0 61 124 302 720.2 0 302 125 336 720.2 0 336 126 520 720.2 0 520 127 654 720.2 0 654 128 411 720.2 0 411 129 243 720.2 0 243 130 73 720.2 0 73 131 42 720.2 0 42 132 49 720.2 0 49 133 83 720.2 0 83 134 65 720.2 0 65 135 103 720.2 0 103 136 76 720.2 0 76 137 150 720.2 0 150 138 448 720.2 0 448 139 442 720.2 0 442 140 550 720.2 0 550 141 416 720.2 0 416 142 270 720.2 0 270 143 63 720.2 0 63 144 51 720.2 0 51 145 68 720.2 0 68 146 103 720.2 0 103 147 85 720.2 0 85 148 72 720.2 0 72 149 47 720.2 0 47 150 43 720.2 0 43 151 182 720.2 0 182 152 154 720.2 0 154 153 279 720.2 0 279 154 87 720.2 0 87 155 113 720.2 0 113 156 81 720.2 0 81 157 64 720.2 0 64 158 343 720.2 0 343 159 451 720.2 0 451 160 588 720.2 0 588 161 449 720.2 0 449 162 98 720.2 0 98 163 104 720.2 0 104 164 73 720.2 0 73 165 59 720.2 0 59 166 33 720.2 0 33 167 85 720.2 0 85 168 61 720.2 0 61 169 230 720.2 0 230 170 56 720.2 0 56 171 332 720.2 0 332 172 55 720.2 0 55 173 48 720.2 0 48 174 70 720.2 0 70 175 58 720.2 0 58 176 70 720.2 0 70 177 71 720.2 0 71 178 34 720.2 0 34 179 58 720.2 0 58 180 47 720.2 0 47 181 113 720.2 0 113 182 120 720.2 0 120 183 158 720.2 0 158 184 86 720.2 0 86 185 61 720.2 0 61 186 76 720.2 0 76 187 70 720.2 0 70 188 235 720.2 0 235 189 124 720.2 0 124 190 355 720.2 0 355 191 42 720.2 0 42 192 59 720.2 0 59 193 48 720.2 0 48 194 56 720.2 0 56 195 59 720.2 0 59 196 42 720.2 0 42 197 52 720.2 0 52 198 45 720.2 0 45 199 54 720.2 0 54 200 48 720.2 0 48 201 69 720.2 0 69 202 92 720.2 0 92 203 44 720.2 0 44 204 44 720.2 0 44 205 58 720.2 0 58 206 46 720.2 0 46 207 76 720.2 0 76 208 93 720.2 0 93 209 85 720.2 0 85 210 49 720.2 0 49 211 68 720.2 0 68 212 71 720.2 0 71 213 72 720.2 0 72 214 148 720.2 0 148 215 100 720.2 0 100 216 197 720.2 0 197 217 53 720.2 0 53 218 90 720.2 0 90 219 108 720.2 0 108 220 436 720.2 0 436 221 404 720.2 0 404 222 425 720.2 0 425 223 255 720.2 0 255 224 1346 720.2 0 1346 225 971 720.2 0 971 226 3330 720.2 0 3330 227 115 720.2 0 115 228 63 720.2 0 63 229 62 720.2 0 62 230 69 720.2 0 69 231 115 720.2 0 115 232 169 720.2 0 169 233 343 720.2 0 343 234 200 720.2 0 200 235 178 720.2 0 178 236 90 720.2 0 90 237 71 720.2 0 71 238 52 720.2 0 52 239 62 720.2 0 62 240 63 720.2 0 63 241 83 720.2 0 83 242 134 720.2 0 134 243 88 720.2 0 88 244 107 720.2 0 107 245 61 720.2 0 61 246 46 720.2 0 46 247 42 720.2 0 42 248 43 720.2 0 43 249 36 720.2 0 36 250 51 720.2 0 51 251 86 720.2 0 86 252 83 720.2 0 83 253 105 720.2 0 105 254 73 720.2 0 73 255 55 720.2 0 55 256 71 720.2 0 71 257 107 720.2 0 107 258 78 720.2 0 78 259 49 720.2 0 49 260 65 720.2 0 65 261 83 720.2 0 83 262 97 720.2 0 97 263 77 720.2 0 77 264 53 720.2 0 53 265 57 720.2 0 57 266 60 720.2 0 60 267 184 720.2 0 184 268 209 720.2 0 209 269 320 720.2 0 320 270 105 720.2 0 105 271 93 720.2 0 93 272 79 720.2 0 79 273 73 720.2 0 73 274 75 720.2 0 75 275 86 720.2 0 86 276 134 720.2 0 134 277 350 720.2 0 350 278 314 720.2 0 314 279 395 720.2 0 395 280 419 720.2 0 419 281 1288 720.2 0 1288 282 1952 720.2 0 1952 283 2311 720.2 0 2311 284 754 720.2 0 754 285 83 720.2 0 83 286 75 720.2 0 75 287 86 720.2 0 86 288 235 720.2 0 235 289 445 720.2 0 445 290 351 720.2 0 351 291 102 720.2 0 102 292 92 720.2 0 92 293 71 720.2 0 71 294 93 720.2 0 93 295 193 720.2 0 193 296 122 720.2 0 122 297 62 720.2 0 62 298 52 720.2 0 52 299 45 720.2 0 45 300 60 720.2 0 60 301 62 720.2 0 62 Finished in 12.31 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 163,775 Reads with adapters: 8,395 (5.1%) Reads that were too short: 74 (0.0%) Reads written (passing filters): 8,321 (5.1%) Total basepairs processed: 45,139,001 bp Total written (filtered): 2,021,132 bp (4.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8395 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 3 0.0 3 0 0 0 3 19 27 0.0 3 0 22 4 1 20 5907 0.0 4 5678 191 26 9 3 21 16 0.0 4 3 10 0 0 3 22 16 0.0 4 6 0 4 1 5 23 73 0.0 4 67 1 0 3 2 24 2 0.0 4 0 0 0 0 2 26 1 0.0 4 0 0 0 1 30 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 78 3 0.0 4 0 0 0 0 3 81 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 109 5 0.0 4 0 0 0 0 5 113 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 120 5 0.0 4 0 0 0 0 5 121 29 0.0 4 0 0 0 0 29 122 2190 0.0 4 0 0 0 0 2190 123 4 0.0 4 0 0 0 0 4 124 3 0.0 4 0 0 0 0 3 125 4 0.0 4 0 0 0 0 4 126 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 130 3 0.0 4 0 0 0 0 3 135 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 148 15 0.0 4 0 0 0 0 15 155 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 163 1 0.0 4 1 165 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 209 4 0.0 4 0 0 0 0 4 213 1 0.0 4 0 0 0 0 1 215 2 0.0 4 0 0 1 0 1 218 1 0.0 4 0 0 0 1 219 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 221 2 0.0 4 0 0 1 0 1 222 1 0.0 4 0 0 0 0 1 223 7 0.0 4 0 0 0 0 7 224 7 0.0 4 0 0 0 0 7 225 2 0.0 4 0 0 0 0 2 227 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 0 6 229 3 0.0 4 0 0 0 0 3 233 1 0.0 4 0 0 0 0 1 249 1 0.0 4 0 0 0 0 1 250 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 257 2 0.0 4 0 0 0 0 2 258 3 0.0 4 0 0 0 0 3 261 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 267 1 0.0 4 0 0 0 1 268 1 0.0 4 0 0 0 0 1 276 2 0.0 4 0 0 0 0 2 279 1 0.0 4 0 0 0 0 1 280 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.48 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 8,321 Reads with adapters: 1,289 (15.5%) Reads written (passing filters): 8,321 (100.0%) Total basepairs processed: 2,021,132 bp Total written (filtered): 1,996,868 bp (98.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1289 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.9% C: 7.9% G: 82.2% T: 4.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 130.0 0 6 4 3 32.5 0 3 9 288 0.0 0 12 276 10 810 0.0 1 36 774 11 105 0.0 1 0 105 12 1 0.0 1 0 1 13 4 0.0 1 0 4 19 1 0.0 1 1 80 3 0.0 1 3 114 1 0.0 1 1 138 1 0.0 1 1 160 41 0.0 1 36 5 161 5 0.0 1 5 162 1 0.0 1 0 1 208 2 0.0 1 2 223 15 0.0 1 13 2 258 1 0.0 1 1 266 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.