This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 140.91 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 150,595 (5.1%) Reads that were too short: 17,846 (0.6%) Reads written (passing filters): 132,749 (4.5%) Total basepairs processed: 887,907,860 bp Total written (filtered): 36,804,884 bp (4.1%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 150595 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 588 720.2 0 588 7 6658 720.2 0 6658 8 270 720.2 0 270 9 7982 720.2 0 7982 10 661 720.2 0 661 11 753 720.2 0 753 12 8669 720.2 0 8669 13 50295 720.2 0 50295 14 44169 720.2 0 44169 15 63 720.2 0 63 16 21 720.2 0 21 17 26 720.2 0 26 18 28 720.2 0 28 19 20 720.2 0 20 20 23 720.2 0 23 21 22 720.2 0 22 22 29 720.2 0 29 23 24 720.2 0 24 24 27 720.2 0 27 25 21 720.2 0 21 26 21 720.2 0 21 27 20 720.2 0 20 28 24 720.2 0 24 29 24 720.2 0 24 30 20 720.2 0 20 31 19 720.2 0 19 32 21 720.2 0 21 33 18 720.2 0 18 34 23 720.2 0 23 35 29 720.2 0 29 36 19 720.2 0 19 37 19 720.2 0 19 38 24 720.2 0 24 39 20 720.2 0 20 40 32 720.2 0 32 41 16 720.2 0 16 42 19 720.2 0 19 43 53 720.2 0 53 44 64 720.2 0 64 45 90 720.2 0 90 46 27 720.2 0 27 47 19 720.2 0 19 48 24 720.2 0 24 49 28 720.2 0 28 50 33 720.2 0 33 51 25 720.2 0 25 52 21 720.2 0 21 53 48 720.2 0 48 54 25 720.2 0 25 55 24 720.2 0 24 56 23 720.2 0 23 57 24 720.2 0 24 58 29 720.2 0 29 59 34 720.2 0 34 60 22 720.2 0 22 61 32 720.2 0 32 62 26 720.2 0 26 63 42 720.2 0 42 64 26 720.2 0 26 65 27 720.2 0 27 66 36 720.2 0 36 67 29 720.2 0 29 68 30 720.2 0 30 69 22 720.2 0 22 70 31 720.2 0 31 71 24 720.2 0 24 72 18 720.2 0 18 73 45 720.2 0 45 74 26 720.2 0 26 75 28 720.2 0 28 76 24 720.2 0 24 77 38 720.2 0 38 78 31 720.2 0 31 79 41 720.2 0 41 80 34 720.2 0 34 81 29 720.2 0 29 82 27 720.2 0 27 83 35 720.2 0 35 84 43 720.2 0 43 85 45 720.2 0 45 86 41 720.2 0 41 87 46 720.2 0 46 88 35 720.2 0 35 89 43 720.2 0 43 90 55 720.2 0 55 91 76 720.2 0 76 92 85 720.2 0 85 93 44 720.2 0 44 94 55 720.2 0 55 95 44 720.2 0 44 96 40 720.2 0 40 97 38 720.2 0 38 98 59 720.2 0 59 99 33 720.2 0 33 100 44 720.2 0 44 101 39 720.2 0 39 102 39 720.2 0 39 103 43 720.2 0 43 104 103 720.2 0 103 105 165 720.2 0 165 106 197 720.2 0 197 107 53 720.2 0 53 108 64 720.2 0 64 109 47 720.2 0 47 110 68 720.2 0 68 111 61 720.2 0 61 112 57 720.2 0 57 113 56 720.2 0 56 114 72 720.2 0 72 115 52 720.2 0 52 116 71 720.2 0 71 117 33 720.2 0 33 118 38 720.2 0 38 119 41 720.2 0 41 120 40 720.2 0 40 121 47 720.2 0 47 122 49 720.2 0 49 123 40 720.2 0 40 124 97 720.2 0 97 125 127 720.2 0 127 126 279 720.2 0 279 127 624 720.2 0 624 128 334 720.2 0 334 129 223 720.2 0 223 130 46 720.2 0 46 131 37 720.2 0 37 132 33 720.2 0 33 133 62 720.2 0 62 134 57 720.2 0 57 135 85 720.2 0 85 136 53 720.2 0 53 137 117 720.2 0 117 138 186 720.2 0 186 139 207 720.2 0 207 140 267 720.2 0 267 141 295 720.2 0 295 142 212 720.2 0 212 143 36 720.2 0 36 144 30 720.2 0 30 145 56 720.2 0 56 146 66 720.2 0 66 147 62 720.2 0 62 148 50 720.2 0 50 149 25 720.2 0 25 150 34 720.2 0 34 151 170 720.2 0 170 152 128 720.2 0 128 153 260 720.2 0 260 154 54 720.2 0 54 155 80 720.2 0 80 156 49 720.2 0 49 157 62 720.2 0 62 158 250 720.2 0 250 159 254 720.2 0 254 160 380 720.2 0 380 161 217 720.2 0 217 162 62 720.2 0 62 163 72 720.2 0 72 164 50 720.2 0 50 165 47 720.2 0 47 166 27 720.2 0 27 167 47 720.2 0 47 168 53 720.2 0 53 169 280 720.2 0 280 170 41 720.2 0 41 171 473 720.2 0 473 172 35 720.2 0 35 173 26 720.2 0 26 174 67 720.2 0 67 175 53 720.2 0 53 176 51 720.2 0 51 177 35 720.2 0 35 178 32 720.2 0 32 179 62 720.2 0 62 180 49 720.2 0 49 181 62 720.2 0 62 182 61 720.2 0 61 183 54 720.2 0 54 184 37 720.2 0 37 185 43 720.2 0 43 186 48 720.2 0 48 187 40 720.2 0 40 188 126 720.2 0 126 189 98 720.2 0 98 190 210 720.2 0 210 191 51 720.2 0 51 192 40 720.2 0 40 193 38 720.2 0 38 194 42 720.2 0 42 195 43 720.2 0 43 196 38 720.2 0 38 197 46 720.2 0 46 198 41 720.2 0 41 199 51 720.2 0 51 200 43 720.2 0 43 201 39 720.2 0 39 202 45 720.2 0 45 203 32 720.2 0 32 204 39 720.2 0 39 205 37 720.2 0 37 206 43 720.2 0 43 207 69 720.2 0 69 208 64 720.2 0 64 209 42 720.2 0 42 210 36 720.2 0 36 211 44 720.2 0 44 212 38 720.2 0 38 213 33 720.2 0 33 214 112 720.2 0 112 215 103 720.2 0 103 216 135 720.2 0 135 217 51 720.2 0 51 218 52 720.2 0 52 219 57 720.2 0 57 220 257 720.2 0 257 221 294 720.2 0 294 222 311 720.2 0 311 223 264 720.2 0 264 224 1357 720.2 0 1357 225 974 720.2 0 974 226 3935 720.2 0 3935 227 90 720.2 0 90 228 34 720.2 0 34 229 36 720.2 0 36 230 47 720.2 0 47 231 53 720.2 0 53 232 97 720.2 0 97 233 273 720.2 0 273 234 226 720.2 0 226 235 211 720.2 0 211 236 89 720.2 0 89 237 65 720.2 0 65 238 38 720.2 0 38 239 34 720.2 0 34 240 44 720.2 0 44 241 58 720.2 0 58 242 84 720.2 0 84 243 44 720.2 0 44 244 59 720.2 0 59 245 43 720.2 0 43 246 38 720.2 0 38 247 39 720.2 0 39 248 28 720.2 0 28 249 36 720.2 0 36 250 40 720.2 0 40 251 57 720.2 0 57 252 47 720.2 0 47 253 78 720.2 0 78 254 57 720.2 0 57 255 54 720.2 0 54 256 48 720.2 0 48 257 70 720.2 0 70 258 52 720.2 0 52 259 49 720.2 0 49 260 51 720.2 0 51 261 71 720.2 0 71 262 75 720.2 0 75 263 58 720.2 0 58 264 52 720.2 0 52 265 41 720.2 0 41 266 41 720.2 0 41 267 84 720.2 0 84 268 105 720.2 0 105 269 203 720.2 0 203 270 92 720.2 0 92 271 58 720.2 0 58 272 62 720.2 0 62 273 59 720.2 0 59 274 72 720.2 0 72 275 54 720.2 0 54 276 129 720.2 0 129 277 380 720.2 0 380 278 349 720.2 0 349 279 453 720.2 0 453 280 196 720.2 0 196 281 569 720.2 0 569 282 835 720.2 0 835 283 1133 720.2 0 1133 284 447 720.2 0 447 285 42 720.2 0 42 286 50 720.2 0 50 287 64 720.2 0 64 288 137 720.2 0 137 289 216 720.2 0 216 290 201 720.2 0 201 291 68 720.2 0 68 292 77 720.2 0 77 293 65 720.2 0 65 294 69 720.2 0 69 295 123 720.2 0 123 296 80 720.2 0 80 297 55 720.2 0 55 298 34 720.2 0 34 299 40 720.2 0 40 300 53 720.2 0 53 301 61 720.2 0 61 Finished in 9.03 s (68 us/read; 0.88 M reads/minute). === Summary === Total reads processed: 132,749 Reads with adapters: 7,069 (5.3%) Reads that were too short: 131 (0.1%) Reads written (passing filters): 6,938 (5.2%) Total basepairs processed: 36,804,884 bp Total written (filtered): 1,804,451 bp (4.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7069 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 23 0.0 3 0 23 17 6015 0.0 3 5910 104 1 18 13 0.0 3 0 13 19 8 0.0 3 4 0 4 20 100 0.0 3 94 1 2 3 21 1 0.0 3 0 1 22 1 0.0 3 1 25 1 0.0 3 1 26 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 1 1 32 11 0.0 3 1 0 0 10 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 1 36 1 0.0 3 1 37 1 0.0 3 0 0 0 1 42 4 0.0 3 0 0 1 3 43 5 0.0 3 0 0 0 5 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 7 0.0 3 0 0 0 7 54 6 0.0 3 0 0 0 6 56 1 0.0 3 0 0 0 1 57 3 0.0 3 0 0 0 3 60 1 0.0 3 0 0 0 1 65 6 0.0 3 0 0 0 6 67 7 0.0 3 0 0 0 7 70 3 0.0 3 0 0 1 2 71 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 84 7 0.0 3 0 0 0 7 88 4 0.0 3 0 0 0 4 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 22 0.0 3 0 0 21 1 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 110 26 0.0 3 0 0 0 26 114 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 124 3 0.0 3 0 0 0 3 126 1 0.0 3 0 0 0 1 129 6 0.0 3 0 0 0 6 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 146 3 0.0 3 0 0 0 3 148 1 0.0 3 0 0 0 1 150 3 0.0 3 0 0 0 3 152 1 0.0 3 0 0 0 1 154 4 0.0 3 0 0 0 4 155 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 159 55 0.0 3 0 0 1 54 160 28 0.0 3 0 0 0 28 161 36 0.0 3 0 0 0 36 162 40 0.0 3 0 0 1 39 163 14 0.0 3 0 0 0 14 164 3 0.0 3 0 0 0 3 165 7 0.0 3 0 0 0 7 167 1 0.0 3 0 0 0 1 168 3 0.0 3 1 0 0 2 171 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 178 9 0.0 3 0 0 0 9 179 321 0.0 3 0 0 0 321 180 52 0.0 3 0 0 0 52 182 34 0.0 3 0 0 0 34 183 3 0.0 3 0 0 0 3 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 3 0.0 3 0 0 0 3 188 7 0.0 3 0 0 0 7 189 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 195 4 0.0 3 0 0 0 4 199 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 2 0.0 3 0 0 0 2 228 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 230 7 0.0 3 0 0 0 7 232 1 0.0 3 0 0 0 1 239 9 0.0 3 0 0 0 9 240 3 0.0 3 0 0 0 3 246 2 0.0 3 0 0 0 2 253 1 0.0 3 0 0 0 1 254 3 0.0 3 0 0 0 3 261 1 0.0 3 0 0 0 1 263 3 0.0 3 0 0 0 3 264 2 0.0 3 0 0 0 2 266 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 270 2 0.0 3 0 1 0 1 271 16 0.0 3 0 0 0 16 272 3 0.0 3 0 0 0 3 273 4 0.0 3 0 0 0 4 274 9 0.0 3 0 0 0 9 275 10 0.0 3 0 0 0 10 276 3 0.0 3 0 0 0 3 277 4 0.0 3 0 0 0 4 280 8 0.0 3 0 0 0 8 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 286 2 0.0 3 0 0 0 2 287 3 0.0 3 0 0 0 3 291 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.38 s (55 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 6,938 Reads with adapters: 37 (0.5%) Reads written (passing filters): 6,938 (100.0%) Total basepairs processed: 1,804,451 bp Total written (filtered): 1,804,337 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 37 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 70.3% C: 16.2% G: 5.4% T: 8.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 34 108.4 0 34 4 3 27.1 0 3