This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 140.50 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 74,722 (2.5%) Reads that were too short: 13,974 (0.5%) Reads written (passing filters): 60,748 (2.1%) Total basepairs processed: 887,907,860 bp Total written (filtered): 15,816,261 bp (1.8%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 74722 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 577 720.2 0 577 7 20077 720.2 0 20077 8 229 720.2 0 229 9 22595 720.2 0 22595 10 71 720.2 0 71 11 98 720.2 0 98 12 25 720.2 0 25 13 42 720.2 0 42 14 38 720.2 0 38 15 46 720.2 0 46 16 38 720.2 0 38 17 51 720.2 0 51 18 30 720.2 0 30 19 38 720.2 0 38 20 46 720.2 0 46 21 41 720.2 0 41 22 50 720.2 0 50 23 42 720.2 0 42 24 43 720.2 0 43 25 46 720.2 0 46 26 33 720.2 0 33 27 27 720.2 0 27 28 25 720.2 0 25 29 69 720.2 0 69 30 36 720.2 0 36 31 38 720.2 0 38 32 39 720.2 0 39 33 36 720.2 0 36 34 35 720.2 0 35 35 36 720.2 0 36 36 31 720.2 0 31 37 47 720.2 0 47 38 42 720.2 0 42 39 47 720.2 0 47 40 40 720.2 0 40 41 36 720.2 0 36 42 70 720.2 0 70 43 28 720.2 0 28 44 43 720.2 0 43 45 39 720.2 0 39 46 46 720.2 0 46 47 31 720.2 0 31 48 35 720.2 0 35 49 48 720.2 0 48 50 31 720.2 0 31 51 24 720.2 0 24 52 49 720.2 0 49 53 56 720.2 0 56 54 44 720.2 0 44 55 56 720.2 0 56 56 49 720.2 0 49 57 46 720.2 0 46 58 57 720.2 0 57 59 35 720.2 0 35 60 44 720.2 0 44 61 36 720.2 0 36 62 27 720.2 0 27 63 34 720.2 0 34 64 41 720.2 0 41 65 50 720.2 0 50 66 34 720.2 0 34 67 53 720.2 0 53 68 65 720.2 0 65 69 46 720.2 0 46 70 57 720.2 0 57 71 67 720.2 0 67 72 34 720.2 0 34 73 129 720.2 0 129 74 79 720.2 0 79 75 138 720.2 0 138 76 44 720.2 0 44 77 39 720.2 0 39 78 38 720.2 0 38 79 50 720.2 0 50 80 50 720.2 0 50 81 45 720.2 0 45 82 49 720.2 0 49 83 37 720.2 0 37 84 48 720.2 0 48 85 49 720.2 0 49 86 45 720.2 0 45 87 60 720.2 0 60 88 45 720.2 0 45 89 41 720.2 0 41 90 44 720.2 0 44 91 49 720.2 0 49 92 41 720.2 0 41 93 34 720.2 0 34 94 39 720.2 0 39 95 51 720.2 0 51 96 58 720.2 0 58 97 53 720.2 0 53 98 31 720.2 0 31 99 67 720.2 0 67 100 62 720.2 0 62 101 520 720.2 0 520 102 296 720.2 0 296 103 422 720.2 0 422 104 69 720.2 0 69 105 88 720.2 0 88 106 54 720.2 0 54 107 45 720.2 0 45 108 52 720.2 0 52 109 35 720.2 0 35 110 66 720.2 0 66 111 323 720.2 0 323 112 249 720.2 0 249 113 505 720.2 0 505 114 89 720.2 0 89 115 99 720.2 0 99 116 125 720.2 0 125 117 92 720.2 0 92 118 42 720.2 0 42 119 57 720.2 0 57 120 48 720.2 0 48 121 50 720.2 0 50 122 47 720.2 0 47 123 104 720.2 0 104 124 106 720.2 0 106 125 98 720.2 0 98 126 82 720.2 0 82 127 57 720.2 0 57 128 48 720.2 0 48 129 40 720.2 0 40 130 69 720.2 0 69 131 94 720.2 0 94 132 411 720.2 0 411 133 114 720.2 0 114 134 232 720.2 0 232 135 43 720.2 0 43 136 78 720.2 0 78 137 140 720.2 0 140 138 150 720.2 0 150 139 133 720.2 0 133 140 75 720.2 0 75 141 140 720.2 0 140 142 116 720.2 0 116 143 149 720.2 0 149 144 445 720.2 0 445 145 260 720.2 0 260 146 646 720.2 0 646 147 68 720.2 0 68 148 112 720.2 0 112 149 626 720.2 0 626 150 88 720.2 0 88 151 121 720.2 0 121 152 60 720.2 0 60 153 46 720.2 0 46 154 192 720.2 0 192 155 94 720.2 0 94 156 256 720.2 0 256 157 67 720.2 0 67 158 115 720.2 0 115 159 93 720.2 0 93 160 103 720.2 0 103 161 86 720.2 0 86 162 58 720.2 0 58 163 83 720.2 0 83 164 102 720.2 0 102 165 66 720.2 0 66 166 88 720.2 0 88 167 51 720.2 0 51 168 107 720.2 0 107 169 131 720.2 0 131 170 365 720.2 0 365 171 207 720.2 0 207 172 389 720.2 0 389 173 42 720.2 0 42 174 43 720.2 0 43 175 55 720.2 0 55 176 46 720.2 0 46 177 74 720.2 0 74 178 140 720.2 0 140 179 178 720.2 0 178 180 152 720.2 0 152 181 73 720.2 0 73 182 57 720.2 0 57 183 48 720.2 0 48 184 63 720.2 0 63 185 78 720.2 0 78 186 68 720.2 0 68 187 73 720.2 0 73 188 52 720.2 0 52 189 57 720.2 0 57 190 58 720.2 0 58 191 40 720.2 0 40 192 51 720.2 0 51 193 50 720.2 0 50 194 62 720.2 0 62 195 59 720.2 0 59 196 44 720.2 0 44 197 69 720.2 0 69 198 81 720.2 0 81 199 98 720.2 0 98 200 63 720.2 0 63 201 67 720.2 0 67 202 72 720.2 0 72 203 80 720.2 0 80 204 154 720.2 0 154 205 158 720.2 0 158 206 134 720.2 0 134 207 75 720.2 0 75 208 67 720.2 0 67 209 79 720.2 0 79 210 235 720.2 0 235 211 116 720.2 0 116 212 168 720.2 0 168 213 73 720.2 0 73 214 80 720.2 0 80 215 53 720.2 0 53 216 61 720.2 0 61 217 58 720.2 0 58 218 69 720.2 0 69 219 90 720.2 0 90 220 60 720.2 0 60 221 54 720.2 0 54 222 65 720.2 0 65 223 96 720.2 0 96 224 166 720.2 0 166 225 189 720.2 0 189 226 191 720.2 0 191 227 230 720.2 0 230 228 150 720.2 0 150 229 108 720.2 0 108 230 71 720.2 0 71 231 56 720.2 0 56 232 60 720.2 0 60 233 59 720.2 0 59 234 64 720.2 0 64 235 67 720.2 0 67 236 72 720.2 0 72 237 72 720.2 0 72 238 85 720.2 0 85 239 81 720.2 0 81 240 77 720.2 0 77 241 68 720.2 0 68 242 80 720.2 0 80 243 76 720.2 0 76 244 161 720.2 0 161 245 217 720.2 0 217 246 172 720.2 0 172 247 270 720.2 0 270 248 138 720.2 0 138 249 145 720.2 0 145 250 54 720.2 0 54 251 58 720.2 0 58 252 59 720.2 0 59 253 57 720.2 0 57 254 70 720.2 0 70 255 73 720.2 0 73 256 98 720.2 0 98 257 165 720.2 0 165 258 169 720.2 0 169 259 155 720.2 0 155 260 109 720.2 0 109 261 93 720.2 0 93 262 95 720.2 0 95 263 77 720.2 0 77 264 203 720.2 0 203 265 204 720.2 0 204 266 349 720.2 0 349 267 92 720.2 0 92 268 82 720.2 0 82 269 66 720.2 0 66 270 79 720.2 0 79 271 80 720.2 0 80 272 124 720.2 0 124 273 183 720.2 0 183 274 121 720.2 0 121 275 86 720.2 0 86 276 72 720.2 0 72 277 114 720.2 0 114 278 102 720.2 0 102 279 95 720.2 0 95 280 126 720.2 0 126 281 110 720.2 0 110 282 162 720.2 0 162 283 203 720.2 0 203 284 587 720.2 0 587 285 754 720.2 0 754 286 1294 720.2 0 1294 287 619 720.2 0 619 288 155 720.2 0 155 289 67 720.2 0 67 290 54 720.2 0 54 291 98 720.2 0 98 292 196 720.2 0 196 293 202 720.2 0 202 294 103 720.2 0 103 295 75 720.2 0 75 296 95 720.2 0 95 297 68 720.2 0 68 298 84 720.2 0 84 299 93 720.2 0 93 300 84 720.2 0 84 301 139 720.2 0 139 Finished in 4.66 s (77 us/read; 0.78 M reads/minute). === Summary === Total reads processed: 60,748 Reads with adapters: 20,693 (34.1%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 20,675 (34.0%) Total basepairs processed: 15,816,261 bp Total written (filtered): 5,616,327 bp (35.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 20693 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 4 0.0 3 0 0 2 0 2 19 121 0.0 3 0 88 14 13 6 20 19888 0.0 4 18876 830 130 42 10 21 51 0.0 4 10 38 1 1 1 22 7 0.0 4 2 0 4 0 1 23 157 0.0 4 127 8 0 15 7 44 1 0.0 4 1 54 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 105 2 0.0 4 0 0 0 0 2 122 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 5 0.0 4 0 0 0 0 5 127 432 0.0 4 0 0 0 0 432 130 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 3 0.0 4 0 0 0 0 3 235 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 260 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.17 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 20,675 Reads with adapters: 1,417 (6.9%) Reads written (passing filters): 20,675 (100.0%) Total basepairs processed: 5,616,327 bp Total written (filtered): 5,598,981 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1417 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.1% C: 9.4% G: 81.6% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 72 323.0 0 72 4 10 80.8 0 10 5 1 20.2 0 1 9 11 0.1 0 2 9 10 30 0.0 1 1 29 11 1045 0.0 1 22 1023 12 212 0.0 1 7 205 14 2 0.0 1 0 2 15 1 0.0 1 0 1 20 22 0.0 1 15 7 152 1 0.0 1 1 160 1 0.0 1 0 1 191 2 0.0 1 0 2 210 7 0.0 1 4 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.