This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 141.38 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 65,043 (2.2%) Reads that were too short: 8,052 (0.3%) Reads written (passing filters): 56,991 (1.9%) Total basepairs processed: 887,907,860 bp Total written (filtered): 15,369,244 bp (1.7%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 65043 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 579 720.2 0 579 7 26171 720.2 0 26171 8 177 720.2 0 177 9 18460 720.2 0 18460 10 36 720.2 0 36 11 41 720.2 0 41 12 28 720.2 0 28 13 29 720.2 0 29 14 22 720.2 0 22 15 19 720.2 0 19 16 25 720.2 0 25 17 27 720.2 0 27 18 22 720.2 0 22 19 22 720.2 0 22 20 29 720.2 0 29 21 43 720.2 0 43 22 30 720.2 0 30 23 42 720.2 0 42 24 32 720.2 0 32 25 22 720.2 0 22 26 29 720.2 0 29 27 21 720.2 0 21 28 17 720.2 0 17 29 32 720.2 0 32 30 22 720.2 0 22 31 23 720.2 0 23 32 18 720.2 0 18 33 33 720.2 0 33 34 14 720.2 0 14 35 27 720.2 0 27 36 23 720.2 0 23 37 18 720.2 0 18 38 25 720.2 0 25 39 21 720.2 0 21 40 23 720.2 0 23 41 20 720.2 0 20 42 32 720.2 0 32 43 17 720.2 0 17 44 21 720.2 0 21 45 28 720.2 0 28 46 29 720.2 0 29 47 36 720.2 0 36 48 27 720.2 0 27 49 40 720.2 0 40 50 21 720.2 0 21 51 27 720.2 0 27 52 127 720.2 0 127 53 45 720.2 0 45 54 38 720.2 0 38 55 40 720.2 0 40 56 32 720.2 0 32 57 19 720.2 0 19 58 30 720.2 0 30 59 14 720.2 0 14 60 26 720.2 0 26 61 24 720.2 0 24 62 22 720.2 0 22 63 21 720.2 0 21 64 34 720.2 0 34 65 35 720.2 0 35 66 23 720.2 0 23 67 33 720.2 0 33 68 42 720.2 0 42 69 28 720.2 0 28 70 60 720.2 0 60 71 75 720.2 0 75 72 32 720.2 0 32 73 130 720.2 0 130 74 52 720.2 0 52 75 138 720.2 0 138 76 26 720.2 0 26 77 21 720.2 0 21 78 23 720.2 0 23 79 23 720.2 0 23 80 28 720.2 0 28 81 28 720.2 0 28 82 75 720.2 0 75 83 20 720.2 0 20 84 31 720.2 0 31 85 23 720.2 0 23 86 28 720.2 0 28 87 34 720.2 0 34 88 40 720.2 0 40 89 31 720.2 0 31 90 28 720.2 0 28 91 29 720.2 0 29 92 28 720.2 0 28 93 30 720.2 0 30 94 22 720.2 0 22 95 31 720.2 0 31 96 34 720.2 0 34 97 34 720.2 0 34 98 31 720.2 0 31 99 34 720.2 0 34 100 28 720.2 0 28 101 284 720.2 0 284 102 150 720.2 0 150 103 195 720.2 0 195 104 53 720.2 0 53 105 38 720.2 0 38 106 47 720.2 0 47 107 27 720.2 0 27 108 27 720.2 0 27 109 37 720.2 0 37 110 48 720.2 0 48 111 166 720.2 0 166 112 92 720.2 0 92 113 259 720.2 0 259 114 57 720.2 0 57 115 53 720.2 0 53 116 83 720.2 0 83 117 51 720.2 0 51 118 41 720.2 0 41 119 31 720.2 0 31 120 21 720.2 0 21 121 24 720.2 0 24 122 31 720.2 0 31 123 56 720.2 0 56 124 78 720.2 0 78 125 67 720.2 0 67 126 57 720.2 0 57 127 48 720.2 0 48 128 11 720.2 0 11 129 27 720.2 0 27 130 46 720.2 0 46 131 58 720.2 0 58 132 308 720.2 0 308 133 76 720.2 0 76 134 200 720.2 0 200 135 30 720.2 0 30 136 36 720.2 0 36 137 78 720.2 0 78 138 98 720.2 0 98 139 61 720.2 0 61 140 56 720.2 0 56 141 69 720.2 0 69 142 65 720.2 0 65 143 74 720.2 0 74 144 315 720.2 0 315 145 177 720.2 0 177 146 482 720.2 0 482 147 32 720.2 0 32 148 120 720.2 0 120 149 725 720.2 0 725 150 63 720.2 0 63 151 95 720.2 0 95 152 45 720.2 0 45 153 43 720.2 0 43 154 148 720.2 0 148 155 57 720.2 0 57 156 185 720.2 0 185 157 48 720.2 0 48 158 56 720.2 0 56 159 44 720.2 0 44 160 61 720.2 0 61 161 42 720.2 0 42 162 25 720.2 0 25 163 48 720.2 0 48 164 53 720.2 0 53 165 27 720.2 0 27 166 59 720.2 0 59 167 41 720.2 0 41 168 60 720.2 0 60 169 85 720.2 0 85 170 326 720.2 0 326 171 175 720.2 0 175 172 324 720.2 0 324 173 38 720.2 0 38 174 23 720.2 0 23 175 34 720.2 0 34 176 30 720.2 0 30 177 68 720.2 0 68 178 78 720.2 0 78 179 122 720.2 0 122 180 73 720.2 0 73 181 36 720.2 0 36 182 27 720.2 0 27 183 33 720.2 0 33 184 31 720.2 0 31 185 42 720.2 0 42 186 42 720.2 0 42 187 33 720.2 0 33 188 47 720.2 0 47 189 41 720.2 0 41 190 37 720.2 0 37 191 29 720.2 0 29 192 32 720.2 0 32 193 29 720.2 0 29 194 23 720.2 0 23 195 42 720.2 0 42 196 30 720.2 0 30 197 47 720.2 0 47 198 39 720.2 0 39 199 53 720.2 0 53 200 42 720.2 0 42 201 35 720.2 0 35 202 34 720.2 0 34 203 44 720.2 0 44 204 58 720.2 0 58 205 68 720.2 0 68 206 25 720.2 0 25 207 28 720.2 0 28 208 24 720.2 0 24 209 63 720.2 0 63 210 299 720.2 0 299 211 71 720.2 0 71 212 174 720.2 0 174 213 51 720.2 0 51 214 50 720.2 0 50 215 35 720.2 0 35 216 32 720.2 0 32 217 41 720.2 0 41 218 40 720.2 0 40 219 42 720.2 0 42 220 35 720.2 0 35 221 29 720.2 0 29 222 22 720.2 0 22 223 68 720.2 0 68 224 63 720.2 0 63 225 102 720.2 0 102 226 100 720.2 0 100 227 140 720.2 0 140 228 91 720.2 0 91 229 66 720.2 0 66 230 35 720.2 0 35 231 30 720.2 0 30 232 42 720.2 0 42 233 37 720.2 0 37 234 59 720.2 0 59 235 39 720.2 0 39 236 40 720.2 0 40 237 47 720.2 0 47 238 66 720.2 0 66 239 44 720.2 0 44 240 38 720.2 0 38 241 38 720.2 0 38 242 43 720.2 0 43 243 41 720.2 0 41 244 62 720.2 0 62 245 99 720.2 0 99 246 84 720.2 0 84 247 178 720.2 0 178 248 84 720.2 0 84 249 77 720.2 0 77 250 42 720.2 0 42 251 35 720.2 0 35 252 32 720.2 0 32 253 31 720.2 0 31 254 24 720.2 0 24 255 50 720.2 0 50 256 48 720.2 0 48 257 70 720.2 0 70 258 106 720.2 0 106 259 92 720.2 0 92 260 49 720.2 0 49 261 47 720.2 0 47 262 64 720.2 0 64 263 68 720.2 0 68 264 115 720.2 0 115 265 112 720.2 0 112 266 173 720.2 0 173 267 47 720.2 0 47 268 40 720.2 0 40 269 38 720.2 0 38 270 51 720.2 0 51 271 49 720.2 0 49 272 81 720.2 0 81 273 91 720.2 0 91 274 62 720.2 0 62 275 46 720.2 0 46 276 47 720.2 0 47 277 112 720.2 0 112 278 90 720.2 0 90 279 78 720.2 0 78 280 60 720.2 0 60 281 64 720.2 0 64 282 98 720.2 0 98 283 88 720.2 0 88 284 335 720.2 0 335 285 458 720.2 0 458 286 723 720.2 0 723 287 316 720.2 0 316 288 50 720.2 0 50 289 57 720.2 0 57 290 42 720.2 0 42 291 54 720.2 0 54 292 103 720.2 0 103 293 83 720.2 0 83 294 38 720.2 0 38 295 45 720.2 0 45 296 39 720.2 0 39 297 44 720.2 0 44 298 65 720.2 0 65 299 59 720.2 0 59 300 67 720.2 0 67 301 96 720.2 0 96 Finished in 4.19 s (74 us/read; 0.82 M reads/minute). === Summary === Total reads processed: 56,991 Reads with adapters: 18,834 (33.0%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 18,816 (33.0%) Total basepairs processed: 15,369,244 bp Total written (filtered): 5,173,423 bp (33.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 18834 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 16 56 0.0 3 0 53 3 17 18704 0.0 3 18324 367 12 1 18 24 0.0 3 3 20 1 19 10 0.0 3 0 0 6 4 20 11 0.0 3 0 0 0 11 35 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 1 192 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 239 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.13 s (60 us/read; 1.00 M reads/minute). === Summary === Total reads processed: 18,816 Reads with adapters: 708 (3.8%) Reads written (passing filters): 18,816 (100.0%) Total basepairs processed: 5,173,423 bp Total written (filtered): 5,171,280 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 708 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 73.4% C: 3.1% G: 23.0% T: 0.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 689 294.0 0 689 4 19 73.5 0 19