This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 138.10 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 218,960 (7.4%) Reads that were too short: 151,665 (5.1%) Reads written (passing filters): 67,295 (2.3%) Total basepairs processed: 887,907,860 bp Total written (filtered): 16,645,882 bp (1.9%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 218960 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 336 720.2 0 336 7 711 720.2 0 711 8 20180 720.2 0 20180 9 18004 720.2 0 18004 10 34 720.2 0 34 11 23 720.2 0 23 12 117 720.2 0 117 13 24 720.2 0 24 14 7 720.2 0 7 15 22 720.2 0 22 16 18 720.2 0 18 17 16 720.2 0 16 18 15 720.2 0 15 19 16 720.2 0 16 20 21 720.2 0 21 21 13 720.2 0 13 22 15 720.2 0 15 23 21 720.2 0 21 24 29 720.2 0 29 25 21 720.2 0 21 26 20 720.2 0 20 27 28 720.2 0 28 28 36 720.2 0 36 29 16 720.2 0 16 30 16 720.2 0 16 31 17 720.2 0 17 32 28 720.2 0 28 33 30 720.2 0 30 34 18 720.2 0 18 35 17 720.2 0 17 36 25 720.2 0 25 37 18 720.2 0 18 38 27 720.2 0 27 39 28 720.2 0 28 40 22 720.2 0 22 41 14 720.2 0 14 42 37 720.2 0 37 43 101 720.2 0 101 44 444 720.2 0 444 45 59 720.2 0 59 46 34 720.2 0 34 47 15 720.2 0 15 48 25 720.2 0 25 49 25 720.2 0 25 50 47 720.2 0 47 51 28 720.2 0 28 52 20 720.2 0 20 53 17 720.2 0 17 54 29 720.2 0 29 55 34 720.2 0 34 56 104 720.2 0 104 57 270 720.2 0 270 58 141 720.2 0 141 59 26 720.2 0 26 60 39 720.2 0 39 61 59 720.2 0 59 62 40 720.2 0 40 63 21 720.2 0 21 64 46 720.2 0 46 65 35 720.2 0 35 66 19 720.2 0 19 67 48 720.2 0 48 68 64 720.2 0 64 69 496 720.2 0 496 70 247 720.2 0 247 71 892 720.2 0 892 72 44 720.2 0 44 73 49 720.2 0 49 74 50 720.2 0 50 75 63 720.2 0 63 76 102 720.2 0 102 77 41 720.2 0 41 78 78 720.2 0 78 79 63 720.2 0 63 80 32 720.2 0 32 81 37 720.2 0 37 82 42 720.2 0 42 83 44 720.2 0 44 84 60 720.2 0 60 85 47 720.2 0 47 86 57 720.2 0 57 87 75 720.2 0 75 88 117 720.2 0 117 89 1877 720.2 0 1877 90 4660 720.2 0 4660 91 2114 720.2 0 2114 92 34 720.2 0 34 93 28 720.2 0 28 94 31 720.2 0 31 95 32 720.2 0 32 96 44 720.2 0 44 97 145 720.2 0 145 98 122 720.2 0 122 99 116 720.2 0 116 100 70 720.2 0 70 101 32 720.2 0 32 102 65 720.2 0 65 103 28 720.2 0 28 104 35 720.2 0 35 105 42 720.2 0 42 106 26 720.2 0 26 107 37 720.2 0 37 108 45 720.2 0 45 109 48 720.2 0 48 110 34 720.2 0 34 111 77 720.2 0 77 112 55 720.2 0 55 113 41 720.2 0 41 114 23 720.2 0 23 115 17 720.2 0 17 116 23 720.2 0 23 117 27 720.2 0 27 118 23 720.2 0 23 119 19 720.2 0 19 120 34 720.2 0 34 121 22 720.2 0 22 122 37 720.2 0 37 123 27 720.2 0 27 124 48 720.2 0 48 125 29 720.2 0 29 126 23 720.2 0 23 127 96 720.2 0 96 128 25 720.2 0 25 129 28 720.2 0 28 130 23 720.2 0 23 131 40 720.2 0 40 132 100 720.2 0 100 133 51 720.2 0 51 134 82 720.2 0 82 135 72 720.2 0 72 136 115 720.2 0 115 137 1755 720.2 0 1755 138 355 720.2 0 355 139 1858 720.2 0 1858 140 32 720.2 0 32 141 23 720.2 0 23 142 34 720.2 0 34 143 34 720.2 0 34 144 24 720.2 0 24 145 39 720.2 0 39 146 70 720.2 0 70 147 28 720.2 0 28 148 92 720.2 0 92 149 137 720.2 0 137 150 155 720.2 0 155 151 33 720.2 0 33 152 31 720.2 0 31 153 51 720.2 0 51 154 48 720.2 0 48 155 56 720.2 0 56 156 35 720.2 0 35 157 65 720.2 0 65 158 67 720.2 0 67 159 58 720.2 0 58 160 65 720.2 0 65 161 96 720.2 0 96 162 67 720.2 0 67 163 40 720.2 0 40 164 33 720.2 0 33 165 25 720.2 0 25 166 29 720.2 0 29 167 25 720.2 0 25 168 177 720.2 0 177 169 77 720.2 0 77 170 183 720.2 0 183 171 28 720.2 0 28 172 55 720.2 0 55 173 62 720.2 0 62 174 84 720.2 0 84 175 115 720.2 0 115 176 1382 720.2 0 1382 177 509 720.2 0 509 178 3014 720.2 0 3014 179 67 720.2 0 67 180 37 720.2 0 37 181 20 720.2 0 20 182 44 720.2 0 44 183 63 720.2 0 63 184 50 720.2 0 50 185 31 720.2 0 31 186 43 720.2 0 43 187 32 720.2 0 32 188 57 720.2 0 57 189 36 720.2 0 36 190 20 720.2 0 20 191 22 720.2 0 22 192 27 720.2 0 27 193 34 720.2 0 34 194 37 720.2 0 37 195 29 720.2 0 29 196 45 720.2 0 45 197 36 720.2 0 36 198 36 720.2 0 36 199 30 720.2 0 30 200 23 720.2 0 23 201 42 720.2 0 42 202 90 720.2 0 90 203 137 720.2 0 137 204 115 720.2 0 115 205 98 720.2 0 98 206 62 720.2 0 62 207 108 720.2 0 108 208 138 720.2 0 138 209 101 720.2 0 101 210 137 720.2 0 137 211 116 720.2 0 116 212 100 720.2 0 100 213 86 720.2 0 86 214 84 720.2 0 84 215 88 720.2 0 88 216 108 720.2 0 108 217 110 720.2 0 110 218 168 720.2 0 168 219 323 720.2 0 323 220 2985 720.2 0 2985 221 1561 720.2 0 1561 222 3987 720.2 0 3987 223 163 720.2 0 163 224 54 720.2 0 54 225 96 720.2 0 96 226 54 720.2 0 54 227 88 720.2 0 88 228 33 720.2 0 33 229 25 720.2 0 25 230 94 720.2 0 94 231 154 720.2 0 154 232 209 720.2 0 209 233 113 720.2 0 113 234 55 720.2 0 55 235 80 720.2 0 80 236 65 720.2 0 65 237 62 720.2 0 62 238 54 720.2 0 54 239 81 720.2 0 81 240 174 720.2 0 174 241 222 720.2 0 222 242 182 720.2 0 182 243 130 720.2 0 130 244 154 720.2 0 154 245 186 720.2 0 186 246 99 720.2 0 99 247 51 720.2 0 51 248 81 720.2 0 81 249 134 720.2 0 134 250 89 720.2 0 89 251 92 720.2 0 92 252 69 720.2 0 69 253 100 720.2 0 100 254 135 720.2 0 135 255 169 720.2 0 169 256 219 720.2 0 219 257 164 720.2 0 164 258 118 720.2 0 118 259 86 720.2 0 86 260 129 720.2 0 129 261 165 720.2 0 165 262 310 720.2 0 310 263 436 720.2 0 436 264 555 720.2 0 555 265 519 720.2 0 519 266 266 720.2 0 266 267 163 720.2 0 163 268 242 720.2 0 242 269 714 720.2 0 714 270 1473 720.2 0 1473 271 1318 720.2 0 1318 272 424 720.2 0 424 273 289 720.2 0 289 274 2949 720.2 0 2949 275 4418 720.2 0 4418 276 2320 720.2 0 2320 277 246 720.2 0 246 278 429 720.2 0 429 279 625 720.2 0 625 280 831 720.2 0 831 281 1908 720.2 0 1908 282 5895 720.2 0 5895 283 28406 720.2 0 28406 284 30291 720.2 0 30291 285 45722 720.2 0 45722 286 3515 720.2 0 3515 287 65 720.2 0 65 288 260 720.2 0 260 289 393 720.2 0 393 290 680 720.2 0 680 291 337 720.2 0 337 292 103 720.2 0 103 293 36 720.2 0 36 294 29 720.2 0 29 295 43 720.2 0 43 296 51 720.2 0 51 297 75 720.2 0 75 298 83 720.2 0 83 299 49 720.2 0 49 300 39 720.2 0 39 301 28 720.2 0 28 Finished in 4.94 s (73 us/read; 0.82 M reads/minute). === Summary === Total reads processed: 67,295 Reads with adapters: 19,068 (28.3%) Reads that were too short: 64 (0.1%) Reads written (passing filters): 19,004 (28.2%) Total basepairs processed: 16,645,882 bp Total written (filtered): 5,122,228 bp (30.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 19068 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 4 0.0 3 0 0 3 1 19 73 0.0 3 0 53 11 8 1 20 18393 0.0 4 17531 702 111 30 19 21 42 0.0 4 9 33 22 6 0.0 4 1 0 5 23 38 0.0 4 0 0 0 30 8 24 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 126 9 0.0 4 0 0 0 0 9 127 428 0.0 4 0 0 0 0 428 128 1 0.0 4 0 0 0 0 1 130 2 0.0 4 1 0 0 0 1 131 3 0.0 4 0 0 0 0 3 135 1 0.0 4 0 0 0 0 1 146 23 0.0 4 0 0 0 2 21 147 9 0.0 4 0 0 0 1 8 148 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 206 5 0.0 4 0 0 0 1 4 210 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 233 2 0.0 4 0 0 0 0 2 234 2 0.0 4 0 0 0 0 2 235 2 0.0 4 0 0 0 0 2 236 1 0.0 4 0 0 0 0 1 240 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.21 s (64 us/read; 0.94 M reads/minute). === Summary === Total reads processed: 19,004 Reads with adapters: 5,238 (27.6%) Reads written (passing filters): 19,004 (100.0%) Total basepairs processed: 5,122,228 bp Total written (filtered): 5,069,544 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5238 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 6.2% G: 91.2% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 296.9 0 5 4 1 74.2 0 1 7 1 1.2 0 1 8 2 0.3 0 2 9 2798 0.1 0 169 2629 10 1941 0.0 1 110 1831 11 456 0.0 1 12 444 12 13 0.0 1 0 13 13 6 0.0 1 0 6 14 1 0.0 1 0 1 34 1 0.0 1 1 150 1 0.0 1 1 208 4 0.0 1 2 2 209 1 0.0 1 1 223 7 0.0 1 7 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.