This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 139.39 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 217,939 (7.4%) Reads that were too short: 151,626 (5.1%) Reads written (passing filters): 66,313 (2.2%) Total basepairs processed: 887,907,860 bp Total written (filtered): 16,530,346 bp (1.9%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 217939 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 341 720.2 0 341 7 535 720.2 0 535 8 18475 720.2 0 18475 9 21732 720.2 0 21732 10 29 720.2 0 29 11 7 720.2 0 7 12 24 720.2 0 24 13 11 720.2 0 11 14 7 720.2 0 7 15 6 720.2 0 6 16 5 720.2 0 5 17 3 720.2 0 3 18 9 720.2 0 9 19 3 720.2 0 3 20 14 720.2 0 14 21 29 720.2 0 29 22 13 720.2 0 13 23 7 720.2 0 7 24 6 720.2 0 6 25 11 720.2 0 11 26 14 720.2 0 14 27 7 720.2 0 7 28 14 720.2 0 14 29 17 720.2 0 17 30 11 720.2 0 11 31 12 720.2 0 12 32 14 720.2 0 14 33 9 720.2 0 9 34 10 720.2 0 10 35 6 720.2 0 6 36 8 720.2 0 8 37 16 720.2 0 16 38 13 720.2 0 13 39 20 720.2 0 20 40 15 720.2 0 15 41 13 720.2 0 13 42 27 720.2 0 27 43 105 720.2 0 105 44 504 720.2 0 504 45 76 720.2 0 76 46 22 720.2 0 22 47 11 720.2 0 11 48 13 720.2 0 13 49 15 720.2 0 15 50 33 720.2 0 33 51 20 720.2 0 20 52 14 720.2 0 14 53 8 720.2 0 8 54 16 720.2 0 16 55 22 720.2 0 22 56 40 720.2 0 40 57 143 720.2 0 143 58 619 720.2 0 619 59 78 720.2 0 78 60 9 720.2 0 9 61 29 720.2 0 29 62 21 720.2 0 21 63 9 720.2 0 9 64 21 720.2 0 21 65 18 720.2 0 18 66 12 720.2 0 12 67 22 720.2 0 22 68 30 720.2 0 30 69 164 720.2 0 164 70 121 720.2 0 121 71 320 720.2 0 320 72 16 720.2 0 16 73 34 720.2 0 34 74 28 720.2 0 28 75 40 720.2 0 40 76 91 720.2 0 91 77 17 720.2 0 17 78 38 720.2 0 38 79 54 720.2 0 54 80 27 720.2 0 27 81 19 720.2 0 19 82 42 720.2 0 42 83 26 720.2 0 26 84 36 720.2 0 36 85 50 720.2 0 50 86 47 720.2 0 47 87 59 720.2 0 59 88 75 720.2 0 75 89 1556 720.2 0 1556 90 4515 720.2 0 4515 91 2045 720.2 0 2045 92 22 720.2 0 22 93 22 720.2 0 22 94 20 720.2 0 20 95 10 720.2 0 10 96 10 720.2 0 10 97 59 720.2 0 59 98 42 720.2 0 42 99 48 720.2 0 48 100 37 720.2 0 37 101 28 720.2 0 28 102 42 720.2 0 42 103 10 720.2 0 10 104 23 720.2 0 23 105 21 720.2 0 21 106 10 720.2 0 10 107 24 720.2 0 24 108 23 720.2 0 23 109 22 720.2 0 22 110 23 720.2 0 23 111 43 720.2 0 43 112 35 720.2 0 35 113 13 720.2 0 13 114 8 720.2 0 8 115 11 720.2 0 11 116 8 720.2 0 8 117 12 720.2 0 12 118 14 720.2 0 14 119 28 720.2 0 28 120 27 720.2 0 27 121 16 720.2 0 16 122 25 720.2 0 25 123 16 720.2 0 16 124 34 720.2 0 34 125 23 720.2 0 23 126 15 720.2 0 15 127 65 720.2 0 65 128 26 720.2 0 26 129 17 720.2 0 17 130 10 720.2 0 10 131 18 720.2 0 18 132 56 720.2 0 56 133 47 720.2 0 47 134 37 720.2 0 37 135 80 720.2 0 80 136 92 720.2 0 92 137 1835 720.2 0 1835 138 355 720.2 0 355 139 1871 720.2 0 1871 140 22 720.2 0 22 141 9 720.2 0 9 142 15 720.2 0 15 143 18 720.2 0 18 144 25 720.2 0 25 145 11 720.2 0 11 146 10 720.2 0 10 147 9 720.2 0 9 148 37 720.2 0 37 149 59 720.2 0 59 150 66 720.2 0 66 151 15 720.2 0 15 152 5 720.2 0 5 153 22 720.2 0 22 154 21 720.2 0 21 155 28 720.2 0 28 156 22 720.2 0 22 157 25 720.2 0 25 158 35 720.2 0 35 159 42 720.2 0 42 160 39 720.2 0 39 161 64 720.2 0 64 162 40 720.2 0 40 163 34 720.2 0 34 164 16 720.2 0 16 165 13 720.2 0 13 166 17 720.2 0 17 167 34 720.2 0 34 168 194 720.2 0 194 169 64 720.2 0 64 170 211 720.2 0 211 171 23 720.2 0 23 172 57 720.2 0 57 173 55 720.2 0 55 174 74 720.2 0 74 175 100 720.2 0 100 176 1740 720.2 0 1740 177 574 720.2 0 574 178 3835 720.2 0 3835 179 33 720.2 0 33 180 16 720.2 0 16 181 16 720.2 0 16 182 18 720.2 0 18 183 18 720.2 0 18 184 19 720.2 0 19 185 19 720.2 0 19 186 30 720.2 0 30 187 13 720.2 0 13 188 28 720.2 0 28 189 27 720.2 0 27 190 21 720.2 0 21 191 13 720.2 0 13 192 24 720.2 0 24 193 33 720.2 0 33 194 14 720.2 0 14 195 23 720.2 0 23 196 26 720.2 0 26 197 13 720.2 0 13 198 23 720.2 0 23 199 14 720.2 0 14 200 17 720.2 0 17 201 19 720.2 0 19 202 58 720.2 0 58 203 80 720.2 0 80 204 45 720.2 0 45 205 38 720.2 0 38 206 44 720.2 0 44 207 71 720.2 0 71 208 111 720.2 0 111 209 63 720.2 0 63 210 61 720.2 0 61 211 44 720.2 0 44 212 37 720.2 0 37 213 40 720.2 0 40 214 30 720.2 0 30 215 71 720.2 0 71 216 85 720.2 0 85 217 113 720.2 0 113 218 132 720.2 0 132 219 263 720.2 0 263 220 3478 720.2 0 3478 221 1384 720.2 0 1384 222 3953 720.2 0 3953 223 120 720.2 0 120 224 26 720.2 0 26 225 79 720.2 0 79 226 31 720.2 0 31 227 57 720.2 0 57 228 16 720.2 0 16 229 23 720.2 0 23 230 46 720.2 0 46 231 75 720.2 0 75 232 77 720.2 0 77 233 41 720.2 0 41 234 39 720.2 0 39 235 41 720.2 0 41 236 35 720.2 0 35 237 33 720.2 0 33 238 31 720.2 0 31 239 65 720.2 0 65 240 97 720.2 0 97 241 105 720.2 0 105 242 89 720.2 0 89 243 67 720.2 0 67 244 120 720.2 0 120 245 105 720.2 0 105 246 85 720.2 0 85 247 38 720.2 0 38 248 54 720.2 0 54 249 87 720.2 0 87 250 73 720.2 0 73 251 88 720.2 0 88 252 35 720.2 0 35 253 81 720.2 0 81 254 109 720.2 0 109 255 110 720.2 0 110 256 184 720.2 0 184 257 118 720.2 0 118 258 107 720.2 0 107 259 49 720.2 0 49 260 118 720.2 0 118 261 163 720.2 0 163 262 137 720.2 0 137 263 167 720.2 0 167 264 219 720.2 0 219 265 206 720.2 0 206 266 217 720.2 0 217 267 124 720.2 0 124 268 147 720.2 0 147 269 470 720.2 0 470 270 818 720.2 0 818 271 837 720.2 0 837 272 287 720.2 0 287 273 255 720.2 0 255 274 3937 720.2 0 3937 275 5894 720.2 0 5894 276 3117 720.2 0 3117 277 200 720.2 0 200 278 343 720.2 0 343 279 648 720.2 0 648 280 690 720.2 0 690 281 1984 720.2 0 1984 282 4401 720.2 0 4401 283 26586 720.2 0 26586 284 31679 720.2 0 31679 285 51336 720.2 0 51336 286 2480 720.2 0 2480 287 64 720.2 0 64 288 128 720.2 0 128 289 190 720.2 0 190 290 338 720.2 0 338 291 173 720.2 0 173 292 112 720.2 0 112 293 34 720.2 0 34 294 35 720.2 0 35 295 98 720.2 0 98 296 20 720.2 0 20 297 43 720.2 0 43 298 32 720.2 0 32 299 32 720.2 0 32 300 24 720.2 0 24 301 6 720.2 0 6 Finished in 4.58 s (69 us/read; 0.87 M reads/minute). === Summary === Total reads processed: 66,313 Reads with adapters: 18,830 (28.4%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 18,816 (28.4%) Total basepairs processed: 16,530,346 bp Total written (filtered): 5,171,852 bp (31.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 18830 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 2 0.0 3 0 0 2 16 82 0.0 3 0 81 1 17 18530 0.0 3 18174 346 9 1 18 42 0.0 3 0 40 2 19 13 0.0 3 0 0 12 1 20 11 0.0 3 0 0 0 11 32 4 0.0 3 0 0 1 3 42 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 46 2 0.0 3 0 0 1 1 78 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 101 104 0.0 3 0 0 1 103 102 16 0.0 3 0 0 1 15 103 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 1 113 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 170 2 0.0 3 0 0 0 2 179 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 204 1 0.0 3 0 1 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 1 279 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.07 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 18,816 Reads with adapters: 234 (1.2%) Reads written (passing filters): 18,816 (100.0%) Total basepairs processed: 5,171,852 bp Total written (filtered): 5,171,016 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 234 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 44.9% C: 1.7% G: 50.0% T: 3.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 101 294.0 0 101 4 132 73.5 0 132 5 1 18.4 0 1