This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 139.77 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 272,933 (9.3%) Reads that were too short: 19,039 (0.6%) Reads written (passing filters): 253,894 (8.6%) Total basepairs processed: 887,907,860 bp Total written (filtered): 57,064,484 bp (6.4%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 272933 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 667 720.2 0 667 7 51920 720.2 0 51920 8 59735 720.2 0 59735 9 70 720.2 0 70 10 51 720.2 0 51 11 45 720.2 0 45 12 31 720.2 0 31 13 30 720.2 0 30 14 36 720.2 0 36 15 38 720.2 0 38 16 29 720.2 0 29 17 33 720.2 0 33 18 37 720.2 0 37 19 59 720.2 0 59 20 47 720.2 0 47 21 46 720.2 0 46 22 33 720.2 0 33 23 33 720.2 0 33 24 38 720.2 0 38 25 31 720.2 0 31 26 26 720.2 0 26 27 42 720.2 0 42 28 42 720.2 0 42 29 67 720.2 0 67 30 47 720.2 0 47 31 33 720.2 0 33 32 36 720.2 0 36 33 59 720.2 0 59 34 45 720.2 0 45 35 43 720.2 0 43 36 62 720.2 0 62 37 57 720.2 0 57 38 58 720.2 0 58 39 45 720.2 0 45 40 58 720.2 0 58 41 47 720.2 0 47 42 54 720.2 0 54 43 53 720.2 0 53 44 33 720.2 0 33 45 64 720.2 0 64 46 59 720.2 0 59 47 47 720.2 0 47 48 59 720.2 0 59 49 280 720.2 0 280 50 107 720.2 0 107 51 57 720.2 0 57 52 58 720.2 0 58 53 58 720.2 0 58 54 55 720.2 0 55 55 60 720.2 0 60 56 75 720.2 0 75 57 56 720.2 0 56 58 43 720.2 0 43 59 59 720.2 0 59 60 81 720.2 0 81 61 53 720.2 0 53 62 65 720.2 0 65 63 67 720.2 0 67 64 112 720.2 0 112 65 153 720.2 0 153 66 226 720.2 0 226 67 582 720.2 0 582 68 234 720.2 0 234 69 177 720.2 0 177 70 296 720.2 0 296 71 131 720.2 0 131 72 136 720.2 0 136 73 218 720.2 0 218 74 188 720.2 0 188 75 265 720.2 0 265 76 301 720.2 0 301 77 399 720.2 0 399 78 549 720.2 0 549 79 1305 720.2 0 1305 80 13037 720.2 0 13037 81 26309 720.2 0 26309 82 22084 720.2 0 22084 83 116 720.2 0 116 84 81 720.2 0 81 85 39 720.2 0 39 86 46 720.2 0 46 87 53 720.2 0 53 88 90 720.2 0 90 89 53 720.2 0 53 90 36 720.2 0 36 91 42 720.2 0 42 92 30 720.2 0 30 93 50 720.2 0 50 94 73 720.2 0 73 95 77 720.2 0 77 96 1501 720.2 0 1501 97 79 720.2 0 79 98 41 720.2 0 41 99 63 720.2 0 63 100 28 720.2 0 28 101 31 720.2 0 31 102 29 720.2 0 29 103 52 720.2 0 52 104 40 720.2 0 40 105 39 720.2 0 39 106 36 720.2 0 36 107 40 720.2 0 40 108 34 720.2 0 34 109 41 720.2 0 41 110 38 720.2 0 38 111 79 720.2 0 79 112 45 720.2 0 45 113 34 720.2 0 34 114 31 720.2 0 31 115 35 720.2 0 35 116 34 720.2 0 34 117 60 720.2 0 60 118 66 720.2 0 66 119 95 720.2 0 95 120 105 720.2 0 105 121 273 720.2 0 273 122 194 720.2 0 194 123 54 720.2 0 54 124 92 720.2 0 92 125 86 720.2 0 86 126 75 720.2 0 75 127 171 720.2 0 171 128 317 720.2 0 317 129 351 720.2 0 351 130 83 720.2 0 83 131 134 720.2 0 134 132 48 720.2 0 48 133 66 720.2 0 66 134 52 720.2 0 52 135 70 720.2 0 70 136 47 720.2 0 47 137 55 720.2 0 55 138 59 720.2 0 59 139 65 720.2 0 65 140 59 720.2 0 59 141 59 720.2 0 59 142 57 720.2 0 57 143 82 720.2 0 82 144 212 720.2 0 212 145 162 720.2 0 162 146 429 720.2 0 429 147 63 720.2 0 63 148 95 720.2 0 95 149 144 720.2 0 144 150 90 720.2 0 90 151 37 720.2 0 37 152 38 720.2 0 38 153 48 720.2 0 48 154 58 720.2 0 58 155 43 720.2 0 43 156 37 720.2 0 37 157 34 720.2 0 34 158 37 720.2 0 37 159 42 720.2 0 42 160 38 720.2 0 38 161 47 720.2 0 47 162 82 720.2 0 82 163 55 720.2 0 55 164 79 720.2 0 79 165 71 720.2 0 71 166 64 720.2 0 64 167 182 720.2 0 182 168 83 720.2 0 83 169 71 720.2 0 71 170 68 720.2 0 68 171 107 720.2 0 107 172 79 720.2 0 79 173 111 720.2 0 111 174 195 720.2 0 195 175 349 720.2 0 349 176 116 720.2 0 116 177 142 720.2 0 142 178 199 720.2 0 199 179 105 720.2 0 105 180 118 720.2 0 118 181 194 720.2 0 194 182 266 720.2 0 266 183 266 720.2 0 266 184 431 720.2 0 431 185 407 720.2 0 407 186 461 720.2 0 461 187 461 720.2 0 461 188 5448 720.2 0 5448 189 40210 720.2 0 40210 190 11735 720.2 0 11735 191 740 720.2 0 740 192 361 720.2 0 361 193 146 720.2 0 146 194 206 720.2 0 206 195 72 720.2 0 72 196 182 720.2 0 182 197 79 720.2 0 79 198 42 720.2 0 42 199 51 720.2 0 51 200 36 720.2 0 36 201 138 720.2 0 138 202 393 720.2 0 393 203 56 720.2 0 56 204 97 720.2 0 97 205 73 720.2 0 73 206 1449 720.2 0 1449 207 809 720.2 0 809 208 1153 720.2 0 1153 209 49 720.2 0 49 210 35 720.2 0 35 211 107 720.2 0 107 212 284 720.2 0 284 213 289 720.2 0 289 214 123 720.2 0 123 215 88 720.2 0 88 216 41 720.2 0 41 217 35 720.2 0 35 218 29 720.2 0 29 219 33 720.2 0 33 220 22 720.2 0 22 221 37 720.2 0 37 222 89 720.2 0 89 223 113 720.2 0 113 224 25 720.2 0 25 225 92 720.2 0 92 226 67 720.2 0 67 227 123 720.2 0 123 228 45 720.2 0 45 229 74 720.2 0 74 230 98 720.2 0 98 231 87 720.2 0 87 232 33 720.2 0 33 233 35 720.2 0 35 234 36 720.2 0 36 235 54 720.2 0 54 236 44 720.2 0 44 237 35 720.2 0 35 238 37 720.2 0 37 239 24 720.2 0 24 240 34 720.2 0 34 241 52 720.2 0 52 242 50 720.2 0 50 243 37 720.2 0 37 244 39 720.2 0 39 245 44 720.2 0 44 246 32 720.2 0 32 247 49 720.2 0 49 248 47 720.2 0 47 249 40 720.2 0 40 250 46 720.2 0 46 251 56 720.2 0 56 252 66 720.2 0 66 253 55 720.2 0 55 254 51 720.2 0 51 255 77 720.2 0 77 256 79 720.2 0 79 257 100 720.2 0 100 258 89 720.2 0 89 259 77 720.2 0 77 260 81 720.2 0 81 261 61 720.2 0 61 262 73 720.2 0 73 263 65 720.2 0 65 264 54 720.2 0 54 265 46 720.2 0 46 266 70 720.2 0 70 267 79 720.2 0 79 268 143 720.2 0 143 269 264 720.2 0 264 270 268 720.2 0 268 271 670 720.2 0 670 272 1824 720.2 0 1824 273 1272 720.2 0 1272 274 678 720.2 0 678 275 81 720.2 0 81 276 43 720.2 0 43 277 48 720.2 0 48 278 64 720.2 0 64 279 82 720.2 0 82 280 75 720.2 0 75 281 86 720.2 0 86 282 602 720.2 0 602 283 1746 720.2 0 1746 284 887 720.2 0 887 285 54 720.2 0 54 286 42 720.2 0 42 287 61 720.2 0 61 288 70 720.2 0 70 289 60 720.2 0 60 290 64 720.2 0 64 291 117 720.2 0 117 292 303 720.2 0 303 293 310 720.2 0 310 294 517 720.2 0 517 295 133 720.2 0 133 296 68 720.2 0 68 297 69 720.2 0 69 298 51 720.2 0 51 299 65 720.2 0 65 300 93 720.2 0 93 301 167 720.2 0 167 Finished in 15.98 s (63 us/read; 0.95 M reads/minute). === Summary === Total reads processed: 253,894 Reads with adapters: 52,581 (20.7%) Reads that were too short: 1,216 (0.5%) Reads written (passing filters): 51,365 (20.2%) Total basepairs processed: 57,064,484 bp Total written (filtered): 14,208,223 bp (24.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 52581 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 5 0.0 3 0 0 5 16 269 0.0 3 0 258 11 17 50776 0.0 3 49819 927 23 7 18 95 0.0 3 16 76 3 19 29 0.0 3 5 0 22 2 20 54 0.0 3 0 0 0 54 22 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 44 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 1 52 15 0.0 3 13 2 53 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 62 14 0.0 3 0 0 0 14 64 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 67 4 0.0 3 0 0 0 4 72 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 86 11 0.0 3 0 0 0 11 87 2 0.0 3 0 0 0 2 88 2 0.0 3 1 0 0 1 89 6 0.0 3 0 0 0 6 90 2 0.0 3 2 91 1 0.0 3 0 0 0 1 92 14 0.0 3 0 0 0 14 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 106 11 0.0 3 0 0 0 11 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 25 0.0 3 0 0 0 25 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 129 5 0.0 3 0 0 0 5 130 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 1 146 3 0.0 3 0 0 0 3 150 1 0.0 3 0 0 0 1 151 1151 0.0 3 0 0 5 1146 156 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 171 2 0.0 3 1 0 0 1 172 2 0.0 3 0 0 0 2 174 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 181 1 0.0 3 0 1 190 1 0.0 3 0 0 0 1 191 4 0.0 3 0 0 0 4 192 1 0.0 3 0 0 0 1 193 3 0.0 3 0 0 0 3 194 3 0.0 3 0 0 0 3 196 1 0.0 3 0 0 0 1 201 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 211 2 0.0 3 0 0 0 2 212 3 0.0 3 0 0 0 3 213 3 0.0 3 0 0 0 3 215 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 1 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.98 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 51,365 Reads with adapters: 39 (0.1%) Reads written (passing filters): 51,365 (100.0%) Total basepairs processed: 14,208,223 bp Total written (filtered): 14,208,095 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 39 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 30.8% C: 25.6% G: 23.1% T: 20.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 32 802.6 0 32 4 3 200.6 0 3 5 4 50.2 0 4