This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 135.81 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 152,779 (5.2%) Reads that were too short: 91,408 (3.1%) Reads written (passing filters): 61,371 (2.1%) Total basepairs processed: 887,907,860 bp Total written (filtered): 10,859,006 bp (1.2%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 152779 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 129 720.2 0 129 7 4065 720.2 0 4065 8 89 720.2 0 89 9 83 720.2 0 83 10 87 720.2 0 87 11 93 720.2 0 93 12 81 720.2 0 81 13 121 720.2 0 121 14 91 720.2 0 91 15 74 720.2 0 74 16 68 720.2 0 68 17 63 720.2 0 63 18 62 720.2 0 62 19 84 720.2 0 84 20 70 720.2 0 70 21 64 720.2 0 64 22 68 720.2 0 68 23 86 720.2 0 86 24 72 720.2 0 72 25 80 720.2 0 80 26 91 720.2 0 91 27 76 720.2 0 76 28 75 720.2 0 75 29 60 720.2 0 60 30 77 720.2 0 77 31 70 720.2 0 70 32 66 720.2 0 66 33 74 720.2 0 74 34 83 720.2 0 83 35 76 720.2 0 76 36 59 720.2 0 59 37 65 720.2 0 65 38 74 720.2 0 74 39 74 720.2 0 74 40 76 720.2 0 76 41 67 720.2 0 67 42 73 720.2 0 73 43 78 720.2 0 78 44 114 720.2 0 114 45 85 720.2 0 85 46 82 720.2 0 82 47 72 720.2 0 72 48 64 720.2 0 64 49 54 720.2 0 54 50 67 720.2 0 67 51 60 720.2 0 60 52 70 720.2 0 70 53 55 720.2 0 55 54 86 720.2 0 86 55 72 720.2 0 72 56 88 720.2 0 88 57 96 720.2 0 96 58 80 720.2 0 80 59 83 720.2 0 83 60 74 720.2 0 74 61 64 720.2 0 64 62 69 720.2 0 69 63 86 720.2 0 86 64 82 720.2 0 82 65 65 720.2 0 65 66 82 720.2 0 82 67 75 720.2 0 75 68 81 720.2 0 81 69 96 720.2 0 96 70 85 720.2 0 85 71 78 720.2 0 78 72 90 720.2 0 90 73 109 720.2 0 109 74 210 720.2 0 210 75 107 720.2 0 107 76 826 720.2 0 826 77 271 720.2 0 271 78 621 720.2 0 621 79 87 720.2 0 87 80 71 720.2 0 71 81 73 720.2 0 73 82 81 720.2 0 81 83 81 720.2 0 81 84 61 720.2 0 61 85 76 720.2 0 76 86 51 720.2 0 51 87 69 720.2 0 69 88 70 720.2 0 70 89 60 720.2 0 60 90 45 720.2 0 45 91 84 720.2 0 84 92 62 720.2 0 62 93 76 720.2 0 76 94 73 720.2 0 73 95 63 720.2 0 63 96 77 720.2 0 77 97 75 720.2 0 75 98 90 720.2 0 90 99 87 720.2 0 87 100 87 720.2 0 87 101 87 720.2 0 87 102 78 720.2 0 78 103 90 720.2 0 90 104 104 720.2 0 104 105 95 720.2 0 95 106 108 720.2 0 108 107 305 720.2 0 305 108 197 720.2 0 197 109 393 720.2 0 393 110 116 720.2 0 116 111 97 720.2 0 97 112 83 720.2 0 83 113 122 720.2 0 122 114 96 720.2 0 96 115 117 720.2 0 117 116 99 720.2 0 99 117 109 720.2 0 109 118 100 720.2 0 100 119 139 720.2 0 139 120 213 720.2 0 213 121 241 720.2 0 241 122 111 720.2 0 111 123 235 720.2 0 235 124 356 720.2 0 356 125 331 720.2 0 331 126 156 720.2 0 156 127 161 720.2 0 161 128 168 720.2 0 168 129 231 720.2 0 231 130 214 720.2 0 214 131 206 720.2 0 206 132 275 720.2 0 275 133 940 720.2 0 940 134 5533 720.2 0 5533 135 6122 720.2 0 6122 136 4290 720.2 0 4290 137 212 720.2 0 212 138 122 720.2 0 122 139 220 720.2 0 220 140 166 720.2 0 166 141 231 720.2 0 231 142 195 720.2 0 195 143 114 720.2 0 114 144 111 720.2 0 111 145 183 720.2 0 183 146 150 720.2 0 150 147 670 720.2 0 670 148 272 720.2 0 272 149 468 720.2 0 468 150 121 720.2 0 121 151 141 720.2 0 141 152 165 720.2 0 165 153 196 720.2 0 196 154 1224 720.2 0 1224 155 2432 720.2 0 2432 156 1153 720.2 0 1153 157 6135 720.2 0 6135 158 1905 720.2 0 1905 159 4538 720.2 0 4538 160 209 720.2 0 209 161 260 720.2 0 260 162 221 720.2 0 221 163 120 720.2 0 120 164 248 720.2 0 248 165 171 720.2 0 171 166 338 720.2 0 338 167 182 720.2 0 182 168 96 720.2 0 96 169 90 720.2 0 90 170 63 720.2 0 63 171 73 720.2 0 73 172 95 720.2 0 95 173 109 720.2 0 109 174 114 720.2 0 114 175 100 720.2 0 100 176 100 720.2 0 100 177 105 720.2 0 105 178 84 720.2 0 84 179 69 720.2 0 69 180 94 720.2 0 94 181 99 720.2 0 99 182 90 720.2 0 90 183 98 720.2 0 98 184 127 720.2 0 127 185 102 720.2 0 102 186 158 720.2 0 158 187 92 720.2 0 92 188 115 720.2 0 115 189 110 720.2 0 110 190 91 720.2 0 91 191 126 720.2 0 126 192 100 720.2 0 100 193 101 720.2 0 101 194 81 720.2 0 81 195 58 720.2 0 58 196 94 720.2 0 94 197 72 720.2 0 72 198 58 720.2 0 58 199 71 720.2 0 71 200 111 720.2 0 111 201 92 720.2 0 92 202 106 720.2 0 106 203 95 720.2 0 95 204 98 720.2 0 98 205 105 720.2 0 105 206 118 720.2 0 118 207 127 720.2 0 127 208 114 720.2 0 114 209 85 720.2 0 85 210 84 720.2 0 84 211 93 720.2 0 93 212 79 720.2 0 79 213 137 720.2 0 137 214 143 720.2 0 143 215 171 720.2 0 171 216 232 720.2 0 232 217 100 720.2 0 100 218 135 720.2 0 135 219 111 720.2 0 111 220 124 720.2 0 124 221 126 720.2 0 126 222 109 720.2 0 109 223 106 720.2 0 106 224 80 720.2 0 80 225 164 720.2 0 164 226 136 720.2 0 136 227 141 720.2 0 141 228 128 720.2 0 128 229 126 720.2 0 126 230 105 720.2 0 105 231 110 720.2 0 110 232 145 720.2 0 145 233 157 720.2 0 157 234 159 720.2 0 159 235 97 720.2 0 97 236 81 720.2 0 81 237 76 720.2 0 76 238 108 720.2 0 108 239 102 720.2 0 102 240 89 720.2 0 89 241 102 720.2 0 102 242 103 720.2 0 103 243 135 720.2 0 135 244 98 720.2 0 98 245 98 720.2 0 98 246 83 720.2 0 83 247 89 720.2 0 89 248 71 720.2 0 71 249 85 720.2 0 85 250 103 720.2 0 103 251 147 720.2 0 147 252 339 720.2 0 339 253 271 720.2 0 271 254 365 720.2 0 365 255 140 720.2 0 140 256 88 720.2 0 88 257 126 720.2 0 126 258 93 720.2 0 93 259 116 720.2 0 116 260 116 720.2 0 116 261 111 720.2 0 111 262 130 720.2 0 130 263 128 720.2 0 128 264 109 720.2 0 109 265 91 720.2 0 91 266 136 720.2 0 136 267 103 720.2 0 103 268 266 720.2 0 266 269 280 720.2 0 280 270 679 720.2 0 679 271 726 720.2 0 726 272 472 720.2 0 472 273 539 720.2 0 539 274 340 720.2 0 340 275 351 720.2 0 351 276 883 720.2 0 883 277 1123 720.2 0 1123 278 1732 720.2 0 1732 279 1086 720.2 0 1086 280 236 720.2 0 236 281 295 720.2 0 295 282 492 720.2 0 492 283 244 720.2 0 244 284 254 720.2 0 254 285 356 720.2 0 356 286 198 720.2 0 198 287 188 720.2 0 188 288 311 720.2 0 311 289 448 720.2 0 448 290 787 720.2 0 787 291 608 720.2 0 608 292 532 720.2 0 532 293 706 720.2 0 706 294 1091 720.2 0 1091 295 16622 720.2 0 16622 296 33609 720.2 0 33609 297 17265 720.2 0 17265 298 165 720.2 0 165 299 107 720.2 0 107 300 99 720.2 0 99 301 140 720.2 0 140 Finished in 3.62 s (59 us/read; 1.02 M reads/minute). === Summary === Total reads processed: 61,371 Reads with adapters: 1,956 (3.2%) Reads that were too short: 36 (0.1%) Reads written (passing filters): 1,920 (3.1%) Total basepairs processed: 10,859,006 bp Total written (filtered): 514,430 bp (4.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1956 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 12 0.0 3 0 5 5 0 2 20 1788 0.0 4 1547 209 21 9 2 21 8 0.0 4 1 5 1 0 1 22 2 0.0 4 2 23 1 0.0 4 0 0 0 1 32 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 68 2 0.0 4 0 0 0 0 2 69 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 81 9 0.0 4 0 0 0 0 9 82 3 0.0 4 0 0 0 0 3 83 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 0 2 94 1 0.0 4 0 0 0 0 1 102 3 0.0 4 0 0 0 0 3 106 2 0.0 4 0 0 0 0 2 109 1 0.0 4 0 1 110 1 0.0 4 0 0 0 0 1 117 2 0.0 4 0 0 0 0 2 123 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 97 0.0 4 0 0 0 0 97 136 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 1 216 1 0.0 4 1 229 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.10 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 1,920 Reads with adapters: 145 (7.6%) Reads written (passing filters): 1,920 (100.0%) Total basepairs processed: 514,430 bp Total written (filtered): 512,597 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 145 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.5% C: 10.3% G: 82.8% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 30.0 0 6 5 3 1.9 0 3 10 2 0.0 1 1 1 11 108 0.0 1 0 108 12 24 0.0 1 0 24 152 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.