This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 138.49 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 181,697 (6.2%) Reads that were too short: 116,277 (3.9%) Reads written (passing filters): 65,420 (2.2%) Total basepairs processed: 887,907,860 bp Total written (filtered): 11,629,586 bp (1.3%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 181697 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 144 720.2 0 144 7 4147 720.2 0 4147 8 119 720.2 0 119 9 92 720.2 0 92 10 118 720.2 0 118 11 110 720.2 0 110 12 82 720.2 0 82 13 126 720.2 0 126 14 121 720.2 0 121 15 95 720.2 0 95 16 109 720.2 0 109 17 98 720.2 0 98 18 97 720.2 0 97 19 105 720.2 0 105 20 99 720.2 0 99 21 100 720.2 0 100 22 88 720.2 0 88 23 105 720.2 0 105 24 101 720.2 0 101 25 113 720.2 0 113 26 93 720.2 0 93 27 80 720.2 0 80 28 97 720.2 0 97 29 78 720.2 0 78 30 94 720.2 0 94 31 98 720.2 0 98 32 100 720.2 0 100 33 81 720.2 0 81 34 99 720.2 0 99 35 94 720.2 0 94 36 81 720.2 0 81 37 89 720.2 0 89 38 95 720.2 0 95 39 81 720.2 0 81 40 90 720.2 0 90 41 88 720.2 0 88 42 106 720.2 0 106 43 99 720.2 0 99 44 104 720.2 0 104 45 84 720.2 0 84 46 116 720.2 0 116 47 94 720.2 0 94 48 88 720.2 0 88 49 86 720.2 0 86 50 98 720.2 0 98 51 85 720.2 0 85 52 75 720.2 0 75 53 73 720.2 0 73 54 101 720.2 0 101 55 72 720.2 0 72 56 103 720.2 0 103 57 93 720.2 0 93 58 101 720.2 0 101 59 98 720.2 0 98 60 115 720.2 0 115 61 113 720.2 0 113 62 99 720.2 0 99 63 95 720.2 0 95 64 105 720.2 0 105 65 118 720.2 0 118 66 96 720.2 0 96 67 94 720.2 0 94 68 72 720.2 0 72 69 125 720.2 0 125 70 118 720.2 0 118 71 85 720.2 0 85 72 93 720.2 0 93 73 119 720.2 0 119 74 212 720.2 0 212 75 122 720.2 0 122 76 702 720.2 0 702 77 191 720.2 0 191 78 286 720.2 0 286 79 118 720.2 0 118 80 132 720.2 0 132 81 122 720.2 0 122 82 107 720.2 0 107 83 132 720.2 0 132 84 122 720.2 0 122 85 133 720.2 0 133 86 102 720.2 0 102 87 138 720.2 0 138 88 127 720.2 0 127 89 132 720.2 0 132 90 95 720.2 0 95 91 122 720.2 0 122 92 93 720.2 0 93 93 117 720.2 0 117 94 106 720.2 0 106 95 116 720.2 0 116 96 102 720.2 0 102 97 105 720.2 0 105 98 88 720.2 0 88 99 137 720.2 0 137 100 127 720.2 0 127 101 131 720.2 0 131 102 88 720.2 0 88 103 149 720.2 0 149 104 127 720.2 0 127 105 121 720.2 0 121 106 151 720.2 0 151 107 203 720.2 0 203 108 165 720.2 0 165 109 254 720.2 0 254 110 143 720.2 0 143 111 157 720.2 0 157 112 125 720.2 0 125 113 139 720.2 0 139 114 145 720.2 0 145 115 129 720.2 0 129 116 115 720.2 0 115 117 150 720.2 0 150 118 150 720.2 0 150 119 162 720.2 0 162 120 196 720.2 0 196 121 258 720.2 0 258 122 174 720.2 0 174 123 201 720.2 0 201 124 240 720.2 0 240 125 200 720.2 0 200 126 175 720.2 0 175 127 206 720.2 0 206 128 192 720.2 0 192 129 245 720.2 0 245 130 210 720.2 0 210 131 226 720.2 0 226 132 259 720.2 0 259 133 898 720.2 0 898 134 5513 720.2 0 5513 135 5993 720.2 0 5993 136 4074 720.2 0 4074 137 253 720.2 0 253 138 153 720.2 0 153 139 228 720.2 0 228 140 189 720.2 0 189 141 277 720.2 0 277 142 199 720.2 0 199 143 143 720.2 0 143 144 117 720.2 0 117 145 156 720.2 0 156 146 162 720.2 0 162 147 517 720.2 0 517 148 234 720.2 0 234 149 373 720.2 0 373 150 151 720.2 0 151 151 148 720.2 0 148 152 200 720.2 0 200 153 198 720.2 0 198 154 1257 720.2 0 1257 155 2637 720.2 0 2637 156 1130 720.2 0 1130 157 7527 720.2 0 7527 158 2187 720.2 0 2187 159 5287 720.2 0 5287 160 173 720.2 0 173 161 179 720.2 0 179 162 130 720.2 0 130 163 105 720.2 0 105 164 150 720.2 0 150 165 123 720.2 0 123 166 171 720.2 0 171 167 148 720.2 0 148 168 104 720.2 0 104 169 113 720.2 0 113 170 96 720.2 0 96 171 99 720.2 0 99 172 126 720.2 0 126 173 95 720.2 0 95 174 128 720.2 0 128 175 109 720.2 0 109 176 119 720.2 0 119 177 96 720.2 0 96 178 97 720.2 0 97 179 88 720.2 0 88 180 98 720.2 0 98 181 101 720.2 0 101 182 119 720.2 0 119 183 95 720.2 0 95 184 116 720.2 0 116 185 112 720.2 0 112 186 138 720.2 0 138 187 107 720.2 0 107 188 126 720.2 0 126 189 111 720.2 0 111 190 92 720.2 0 92 191 125 720.2 0 125 192 104 720.2 0 104 193 146 720.2 0 146 194 118 720.2 0 118 195 85 720.2 0 85 196 93 720.2 0 93 197 95 720.2 0 95 198 76 720.2 0 76 199 118 720.2 0 118 200 97 720.2 0 97 201 96 720.2 0 96 202 144 720.2 0 144 203 95 720.2 0 95 204 105 720.2 0 105 205 118 720.2 0 118 206 120 720.2 0 120 207 126 720.2 0 126 208 116 720.2 0 116 209 95 720.2 0 95 210 86 720.2 0 86 211 108 720.2 0 108 212 122 720.2 0 122 213 123 720.2 0 123 214 129 720.2 0 129 215 110 720.2 0 110 216 273 720.2 0 273 217 113 720.2 0 113 218 97 720.2 0 97 219 86 720.2 0 86 220 103 720.2 0 103 221 124 720.2 0 124 222 110 720.2 0 110 223 110 720.2 0 110 224 97 720.2 0 97 225 277 720.2 0 277 226 129 720.2 0 129 227 145 720.2 0 145 228 138 720.2 0 138 229 117 720.2 0 117 230 104 720.2 0 104 231 111 720.2 0 111 232 114 720.2 0 114 233 108 720.2 0 108 234 143 720.2 0 143 235 105 720.2 0 105 236 102 720.2 0 102 237 81 720.2 0 81 238 119 720.2 0 119 239 101 720.2 0 101 240 94 720.2 0 94 241 106 720.2 0 106 242 98 720.2 0 98 243 89 720.2 0 89 244 97 720.2 0 97 245 99 720.2 0 99 246 93 720.2 0 93 247 94 720.2 0 94 248 103 720.2 0 103 249 87 720.2 0 87 250 114 720.2 0 114 251 173 720.2 0 173 252 259 720.2 0 259 253 224 720.2 0 224 254 299 720.2 0 299 255 108 720.2 0 108 256 107 720.2 0 107 257 111 720.2 0 111 258 80 720.2 0 80 259 101 720.2 0 101 260 107 720.2 0 107 261 121 720.2 0 121 262 128 720.2 0 128 263 100 720.2 0 100 264 114 720.2 0 114 265 110 720.2 0 110 266 117 720.2 0 117 267 110 720.2 0 110 268 171 720.2 0 171 269 183 720.2 0 183 270 543 720.2 0 543 271 707 720.2 0 707 272 479 720.2 0 479 273 504 720.2 0 504 274 301 720.2 0 301 275 316 720.2 0 316 276 1005 720.2 0 1005 277 962 720.2 0 962 278 1777 720.2 0 1777 279 1165 720.2 0 1165 280 239 720.2 0 239 281 341 720.2 0 341 282 628 720.2 0 628 283 285 720.2 0 285 284 330 720.2 0 330 285 440 720.2 0 440 286 216 720.2 0 216 287 203 720.2 0 203 288 421 720.2 0 421 289 498 720.2 0 498 290 773 720.2 0 773 291 703 720.2 0 703 292 596 720.2 0 596 293 780 720.2 0 780 294 1114 720.2 0 1114 295 21469 720.2 0 21469 296 46686 720.2 0 46686 297 23962 720.2 0 23962 298 162 720.2 0 162 299 78 720.2 0 78 300 95 720.2 0 95 301 98 720.2 0 98 Finished in 3.60 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 65,420 Reads with adapters: 1,957 (3.0%) Reads that were too short: 29 (0.0%) Reads written (passing filters): 1,928 (2.9%) Total basepairs processed: 11,629,586 bp Total written (filtered): 508,023 bp (4.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1957 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 7 0.0 3 0 7 17 1755 0.0 3 1476 256 23 18 3 0.0 3 0 1 2 19 2 0.0 3 1 0 1 20 5 0.0 3 0 0 0 5 27 2 0.0 3 0 0 0 2 32 10 0.0 3 0 0 0 10 35 2 0.0 3 0 0 0 2 44 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 131 0.0 3 0 0 0 131 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 68 4 0.0 3 0 0 0 4 71 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 1 92 11 0.0 3 0 0 0 11 97 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 130 1 0.0 3 0 1 133 1 0.0 3 0 0 0 1 134 4 0.0 3 0 0 0 4 135 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 1 261 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.12 s (62 us/read; 0.96 M reads/minute). === Summary === Total reads processed: 1,928 Reads with adapters: 3 (0.2%) Reads written (passing filters): 1,928 (100.0%) Total basepairs processed: 508,023 bp Total written (filtered): 508,012 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 33.3% G: 33.3% T: 33.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 30.1 0 1 4 2 7.5 0 2