This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 137.84 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 160,455 (5.4%) Reads that were too short: 18,876 (0.6%) Reads written (passing filters): 141,579 (4.8%) Total basepairs processed: 887,907,860 bp Total written (filtered): 37,287,408 bp (4.2%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 160455 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1301 720.2 0 1301 7 1594 720.2 0 1594 8 57313 720.2 0 57313 9 43697 720.2 0 43697 10 41 720.2 0 41 11 5 720.2 0 5 12 5 720.2 0 5 13 11 720.2 0 11 14 101 720.2 0 101 15 140 720.2 0 140 16 21 720.2 0 21 17 7 720.2 0 7 18 59 720.2 0 59 19 40 720.2 0 40 20 49 720.2 0 49 21 16 720.2 0 16 22 3 720.2 0 3 23 5 720.2 0 5 24 1 720.2 0 1 25 5 720.2 0 5 26 19 720.2 0 19 27 29 720.2 0 29 28 11 720.2 0 11 29 5 720.2 0 5 30 6 720.2 0 6 31 7 720.2 0 7 32 9 720.2 0 9 33 7 720.2 0 7 34 2 720.2 0 2 35 4 720.2 0 4 36 5 720.2 0 5 38 4 720.2 0 4 39 11 720.2 0 11 40 14 720.2 0 14 41 21 720.2 0 21 42 10 720.2 0 10 43 50 720.2 0 50 44 175 720.2 0 175 45 299 720.2 0 299 46 136 720.2 0 136 47 10 720.2 0 10 48 9 720.2 0 9 49 9 720.2 0 9 50 35 720.2 0 35 51 17 720.2 0 17 52 14 720.2 0 14 53 13 720.2 0 13 54 8 720.2 0 8 55 42 720.2 0 42 56 13 720.2 0 13 57 9 720.2 0 9 58 13 720.2 0 13 59 28 720.2 0 28 60 40 720.2 0 40 61 15 720.2 0 15 62 19 720.2 0 19 63 20 720.2 0 20 64 26 720.2 0 26 65 28 720.2 0 28 66 25 720.2 0 25 67 57 720.2 0 57 68 42 720.2 0 42 69 18 720.2 0 18 70 27 720.2 0 27 71 45 720.2 0 45 72 66 720.2 0 66 73 77 720.2 0 77 74 130 720.2 0 130 75 205 720.2 0 205 76 255 720.2 0 255 77 2516 720.2 0 2516 78 10040 720.2 0 10040 79 5075 720.2 0 5075 80 22 720.2 0 22 81 45 720.2 0 45 82 19 720.2 0 19 83 8 720.2 0 8 84 11 720.2 0 11 85 31 720.2 0 31 86 23 720.2 0 23 87 6 720.2 0 6 88 3 720.2 0 3 89 5 720.2 0 5 90 9 720.2 0 9 91 33 720.2 0 33 92 59 720.2 0 59 93 67 720.2 0 67 94 29 720.2 0 29 95 17 720.2 0 17 96 47 720.2 0 47 97 4 720.2 0 4 98 6 720.2 0 6 99 20 720.2 0 20 100 40 720.2 0 40 101 74 720.2 0 74 102 1157 720.2 0 1157 103 40 720.2 0 40 104 9 720.2 0 9 105 12 720.2 0 12 106 5 720.2 0 5 107 5 720.2 0 5 108 11 720.2 0 11 109 6 720.2 0 6 110 3 720.2 0 3 111 6 720.2 0 6 112 12 720.2 0 12 113 7 720.2 0 7 114 32 720.2 0 32 115 72 720.2 0 72 116 118 720.2 0 118 117 24 720.2 0 24 118 12 720.2 0 12 119 13 720.2 0 13 120 32 720.2 0 32 121 20 720.2 0 20 122 51 720.2 0 51 123 33 720.2 0 33 124 9 720.2 0 9 125 9 720.2 0 9 126 21 720.2 0 21 127 18 720.2 0 18 128 18 720.2 0 18 129 58 720.2 0 58 130 21 720.2 0 21 131 17 720.2 0 17 132 16 720.2 0 16 133 13 720.2 0 13 134 39 720.2 0 39 135 74 720.2 0 74 136 182 720.2 0 182 137 491 720.2 0 491 138 132 720.2 0 132 139 61 720.2 0 61 140 54 720.2 0 54 141 28 720.2 0 28 142 21 720.2 0 21 143 64 720.2 0 64 144 51 720.2 0 51 145 14 720.2 0 14 146 45 720.2 0 45 147 11 720.2 0 11 148 13 720.2 0 13 149 23 720.2 0 23 150 40 720.2 0 40 151 113 720.2 0 113 152 26 720.2 0 26 153 14 720.2 0 14 154 12 720.2 0 12 155 13 720.2 0 13 156 18 720.2 0 18 157 32 720.2 0 32 158 121 720.2 0 121 159 25 720.2 0 25 160 34 720.2 0 34 161 27 720.2 0 27 162 75 720.2 0 75 163 93 720.2 0 93 164 71 720.2 0 71 165 79 720.2 0 79 166 95 720.2 0 95 167 17 720.2 0 17 168 29 720.2 0 29 169 26 720.2 0 26 170 43 720.2 0 43 171 44 720.2 0 44 172 79 720.2 0 79 173 735 720.2 0 735 174 1187 720.2 0 1187 175 903 720.2 0 903 176 73 720.2 0 73 177 21 720.2 0 21 178 46 720.2 0 46 179 63 720.2 0 63 180 699 720.2 0 699 181 332 720.2 0 332 182 995 720.2 0 995 183 60 720.2 0 60 184 43 720.2 0 43 185 59 720.2 0 59 186 57 720.2 0 57 187 54 720.2 0 54 188 56 720.2 0 56 189 108 720.2 0 108 190 179 720.2 0 179 191 1956 720.2 0 1956 192 1093 720.2 0 1093 193 3401 720.2 0 3401 194 206 720.2 0 206 195 20 720.2 0 20 196 18 720.2 0 18 197 16 720.2 0 16 198 29 720.2 0 29 199 42 720.2 0 42 200 29 720.2 0 29 201 28 720.2 0 28 202 330 720.2 0 330 203 269 720.2 0 269 204 580 720.2 0 580 205 215 720.2 0 215 206 199 720.2 0 199 207 80 720.2 0 80 208 85 720.2 0 85 209 204 720.2 0 204 210 143 720.2 0 143 211 86 720.2 0 86 212 18 720.2 0 18 213 11 720.2 0 11 214 10 720.2 0 10 215 25 720.2 0 25 216 38 720.2 0 38 217 62 720.2 0 62 218 48 720.2 0 48 219 30 720.2 0 30 220 17 720.2 0 17 221 15 720.2 0 15 222 6 720.2 0 6 223 10 720.2 0 10 224 10 720.2 0 10 225 19 720.2 0 19 226 25 720.2 0 25 227 29 720.2 0 29 228 34 720.2 0 34 229 118 720.2 0 118 230 21 720.2 0 21 231 14 720.2 0 14 232 15 720.2 0 15 233 33 720.2 0 33 234 39 720.2 0 39 235 37 720.2 0 37 236 37 720.2 0 37 237 28 720.2 0 28 238 20 720.2 0 20 239 20 720.2 0 20 240 17 720.2 0 17 241 15 720.2 0 15 242 22 720.2 0 22 243 28 720.2 0 28 244 21 720.2 0 21 245 24 720.2 0 24 246 27 720.2 0 27 247 28 720.2 0 28 248 24 720.2 0 24 249 34 720.2 0 34 250 24 720.2 0 24 251 66 720.2 0 66 252 202 720.2 0 202 253 391 720.2 0 391 254 473 720.2 0 473 255 108 720.2 0 108 256 29 720.2 0 29 257 36 720.2 0 36 258 93 720.2 0 93 259 268 720.2 0 268 260 722 720.2 0 722 261 1497 720.2 0 1497 262 612 720.2 0 612 263 139 720.2 0 139 264 141 720.2 0 141 265 126 720.2 0 126 266 198 720.2 0 198 267 42 720.2 0 42 268 31 720.2 0 31 269 22 720.2 0 22 270 53 720.2 0 53 271 104 720.2 0 104 272 93 720.2 0 93 273 107 720.2 0 107 274 71 720.2 0 71 275 32 720.2 0 32 276 35 720.2 0 35 277 69 720.2 0 69 278 78 720.2 0 78 279 124 720.2 0 124 280 116 720.2 0 116 281 104 720.2 0 104 282 66 720.2 0 66 283 194 720.2 0 194 284 1865 720.2 0 1865 285 3257 720.2 0 3257 286 2303 720.2 0 2303 287 282 720.2 0 282 288 59 720.2 0 59 289 36 720.2 0 36 290 48 720.2 0 48 291 71 720.2 0 71 292 41 720.2 0 41 293 136 720.2 0 136 294 40 720.2 0 40 295 231 720.2 0 231 296 350 720.2 0 350 297 207 720.2 0 207 298 114 720.2 0 114 299 35 720.2 0 35 300 26 720.2 0 26 301 89 720.2 0 89 Finished in 10.65 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 141,579 Reads with adapters: 50,352 (35.6%) Reads that were too short: 50 (0.0%) Reads written (passing filters): 50,302 (35.5%) Total basepairs processed: 37,287,408 bp Total written (filtered): 13,384,851 bp (35.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 50352 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 3 1 18 30 0.0 3 0 0 23 5 2 19 798 0.0 3 0 727 44 21 6 20 46357 0.0 4 44222 1734 297 79 25 21 146 0.0 4 50 82 9 1 4 22 19 0.0 4 3 0 12 0 4 23 95 0.0 4 0 0 1 73 21 24 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 1 42 1 0.0 4 0 0 0 0 1 52 1 0.0 4 1 67 3 0.0 4 0 0 0 0 3 70 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 90 4 0.0 4 0 0 0 0 4 91 2 0.0 4 0 0 0 0 2 93 1 0.0 4 0 0 0 0 1 96 2 0.0 4 1 0 0 0 1 108 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 24 0.0 4 0 0 0 0 24 127 2777 0.0 4 0 0 0 0 2777 128 6 0.0 4 0 0 0 0 6 129 1 0.0 4 0 0 0 0 1 130 17 0.0 4 0 0 0 0 17 133 1 0.0 4 0 0 0 0 1 136 2 0.0 4 0 0 0 0 2 145 1 0.0 4 1 146 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 168 2 0.0 4 0 1 0 0 1 171 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 202 2 0.0 4 0 0 0 0 2 209 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 228 9 0.0 4 0 0 0 1 8 229 6 0.0 4 0 0 0 0 6 230 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 235 4 0.0 4 0 0 0 0 4 236 1 0.0 4 0 0 0 0 1 239 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 0 2 264 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.09 s (61 us/read; 0.98 M reads/minute). === Summary === Total reads processed: 50,302 Reads with adapters: 10,770 (21.4%) Reads written (passing filters): 50,302 (100.0%) Total basepairs processed: 13,384,851 bp Total written (filtered): 13,274,611 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 10770 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 5.4% G: 91.6% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 17 786.0 0 17 4 7 196.5 0 7 5 2 49.1 0 2 6 1 12.3 0 1 7 2 3.1 0 2 8 1 0.8 0 1 9 3251 0.2 0 136 3115 10 6041 0.0 1 351 5690 11 1381 0.0 1 24 1357 12 16 0.0 1 1 15 13 21 0.0 1 0 21 14 4 0.0 1 0 4 79 1 0.0 1 0 1 150 8 0.0 1 6 2 152 1 0.0 1 0 1 169 2 0.0 1 2 208 9 0.0 1 9 209 1 0.0 1 1 223 4 0.0 1 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.