This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 136.26 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 152,207 (5.2%) Reads that were too short: 9,692 (0.3%) Reads written (passing filters): 142,515 (4.8%) Total basepairs processed: 887,907,860 bp Total written (filtered): 37,886,251 bp (4.3%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 152207 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1153 720.2 0 1153 7 1371 720.2 0 1371 8 50729 720.2 0 50729 9 53832 720.2 0 53832 10 41 720.2 0 41 11 5 720.2 0 5 12 3 720.2 0 3 13 5 720.2 0 5 14 2 720.2 0 2 15 1 720.2 0 1 16 4 720.2 0 4 18 14 720.2 0 14 19 10 720.2 0 10 20 24 720.2 0 24 21 3 720.2 0 3 22 1 720.2 0 1 23 2 720.2 0 2 24 1 720.2 0 1 25 2 720.2 0 2 27 4 720.2 0 4 28 5 720.2 0 5 29 3 720.2 0 3 30 6 720.2 0 6 31 6 720.2 0 6 32 8 720.2 0 8 33 7 720.2 0 7 34 2 720.2 0 2 35 4 720.2 0 4 36 7 720.2 0 7 37 7 720.2 0 7 38 7 720.2 0 7 39 18 720.2 0 18 40 11 720.2 0 11 41 5 720.2 0 5 42 12 720.2 0 12 43 22 720.2 0 22 44 43 720.2 0 43 45 44 720.2 0 44 46 41 720.2 0 41 47 12 720.2 0 12 48 11 720.2 0 11 49 11 720.2 0 11 50 35 720.2 0 35 51 11 720.2 0 11 52 10 720.2 0 10 53 7 720.2 0 7 54 10 720.2 0 10 55 37 720.2 0 37 56 16 720.2 0 16 57 15 720.2 0 15 58 14 720.2 0 14 59 33 720.2 0 33 60 24 720.2 0 24 61 14 720.2 0 14 62 16 720.2 0 16 63 10 720.2 0 10 64 25 720.2 0 25 65 42 720.2 0 42 66 15 720.2 0 15 67 24 720.2 0 24 68 27 720.2 0 27 69 13 720.2 0 13 70 17 720.2 0 17 71 23 720.2 0 23 72 54 720.2 0 54 73 104 720.2 0 104 74 149 720.2 0 149 75 218 720.2 0 218 76 234 720.2 0 234 77 2604 720.2 0 2604 78 10062 720.2 0 10062 79 6189 720.2 0 6189 80 37 720.2 0 37 81 49 720.2 0 49 82 9 720.2 0 9 83 3 720.2 0 3 84 9 720.2 0 9 85 5 720.2 0 5 86 5 720.2 0 5 87 6 720.2 0 6 88 4 720.2 0 4 89 8 720.2 0 8 90 7 720.2 0 7 91 35 720.2 0 35 92 61 720.2 0 61 93 85 720.2 0 85 94 37 720.2 0 37 95 17 720.2 0 17 96 47 720.2 0 47 97 7 720.2 0 7 98 8 720.2 0 8 99 25 720.2 0 25 100 58 720.2 0 58 101 32 720.2 0 32 102 1422 720.2 0 1422 103 59 720.2 0 59 104 20 720.2 0 20 105 7 720.2 0 7 106 7 720.2 0 7 107 7 720.2 0 7 108 10 720.2 0 10 109 5 720.2 0 5 110 2 720.2 0 2 112 5 720.2 0 5 113 6 720.2 0 6 114 13 720.2 0 13 115 37 720.2 0 37 116 115 720.2 0 115 117 12 720.2 0 12 118 18 720.2 0 18 119 10 720.2 0 10 120 23 720.2 0 23 121 12 720.2 0 12 122 31 720.2 0 31 123 18 720.2 0 18 124 5 720.2 0 5 125 9 720.2 0 9 126 10 720.2 0 10 127 7 720.2 0 7 128 13 720.2 0 13 129 32 720.2 0 32 130 3 720.2 0 3 131 21 720.2 0 21 132 6 720.2 0 6 133 14 720.2 0 14 134 23 720.2 0 23 135 32 720.2 0 32 136 164 720.2 0 164 137 479 720.2 0 479 138 119 720.2 0 119 139 56 720.2 0 56 140 29 720.2 0 29 141 18 720.2 0 18 142 16 720.2 0 16 143 44 720.2 0 44 144 28 720.2 0 28 145 15 720.2 0 15 146 44 720.2 0 44 147 7 720.2 0 7 148 14 720.2 0 14 149 10 720.2 0 10 150 24 720.2 0 24 151 106 720.2 0 106 152 22 720.2 0 22 153 13 720.2 0 13 154 11 720.2 0 11 155 11 720.2 0 11 156 10 720.2 0 10 157 26 720.2 0 26 158 81 720.2 0 81 159 8 720.2 0 8 160 18 720.2 0 18 161 11 720.2 0 11 162 51 720.2 0 51 163 42 720.2 0 42 164 37 720.2 0 37 165 33 720.2 0 33 166 29 720.2 0 29 167 18 720.2 0 18 168 16 720.2 0 16 169 13 720.2 0 13 170 13 720.2 0 13 171 11 720.2 0 11 172 25 720.2 0 25 173 305 720.2 0 305 174 603 720.2 0 603 175 427 720.2 0 427 176 63 720.2 0 63 177 26 720.2 0 26 178 31 720.2 0 31 179 56 720.2 0 56 180 438 720.2 0 438 181 201 720.2 0 201 182 528 720.2 0 528 183 21 720.2 0 21 184 11 720.2 0 11 185 23 720.2 0 23 186 37 720.2 0 37 187 41 720.2 0 41 188 43 720.2 0 43 189 104 720.2 0 104 190 130 720.2 0 130 191 2317 720.2 0 2317 192 1102 720.2 0 1102 193 3926 720.2 0 3926 194 189 720.2 0 189 195 10 720.2 0 10 196 13 720.2 0 13 197 12 720.2 0 12 198 18 720.2 0 18 199 15 720.2 0 15 200 15 720.2 0 15 201 19 720.2 0 19 202 327 720.2 0 327 203 210 720.2 0 210 204 487 720.2 0 487 205 169 720.2 0 169 206 257 720.2 0 257 207 64 720.2 0 64 208 26 720.2 0 26 209 43 720.2 0 43 210 35 720.2 0 35 211 27 720.2 0 27 212 7 720.2 0 7 213 12 720.2 0 12 214 5 720.2 0 5 215 10 720.2 0 10 216 13 720.2 0 13 217 19 720.2 0 19 218 13 720.2 0 13 219 7 720.2 0 7 220 5 720.2 0 5 221 5 720.2 0 5 222 10 720.2 0 10 223 8 720.2 0 8 224 6 720.2 0 6 225 3 720.2 0 3 226 13 720.2 0 13 227 6 720.2 0 6 228 15 720.2 0 15 229 177 720.2 0 177 230 23 720.2 0 23 231 13 720.2 0 13 232 6 720.2 0 6 233 5 720.2 0 5 234 20 720.2 0 20 235 14 720.2 0 14 236 16 720.2 0 16 237 24 720.2 0 24 238 9 720.2 0 9 239 9 720.2 0 9 240 14 720.2 0 14 241 13 720.2 0 13 242 12 720.2 0 12 243 6 720.2 0 6 244 12 720.2 0 12 245 18 720.2 0 18 246 4 720.2 0 4 247 20 720.2 0 20 248 11 720.2 0 11 249 6 720.2 0 6 250 15 720.2 0 15 251 48 720.2 0 48 252 77 720.2 0 77 253 172 720.2 0 172 254 149 720.2 0 149 255 35 720.2 0 35 256 15 720.2 0 15 257 13 720.2 0 13 258 46 720.2 0 46 259 191 720.2 0 191 260 367 720.2 0 367 261 831 720.2 0 831 262 312 720.2 0 312 263 56 720.2 0 56 264 77 720.2 0 77 265 52 720.2 0 52 266 133 720.2 0 133 267 20 720.2 0 20 268 20 720.2 0 20 269 6 720.2 0 6 270 16 720.2 0 16 271 27 720.2 0 27 272 45 720.2 0 45 273 35 720.2 0 35 274 32 720.2 0 32 275 21 720.2 0 21 276 21 720.2 0 21 277 26 720.2 0 26 278 41 720.2 0 41 279 52 720.2 0 52 280 51 720.2 0 51 281 37 720.2 0 37 282 36 720.2 0 36 283 77 720.2 0 77 284 801 720.2 0 801 285 1350 720.2 0 1350 286 1038 720.2 0 1038 287 362 720.2 0 362 288 45 720.2 0 45 289 25 720.2 0 25 290 23 720.2 0 23 291 42 720.2 0 42 292 22 720.2 0 22 293 124 720.2 0 124 294 9 720.2 0 9 295 159 720.2 0 159 296 168 720.2 0 168 297 60 720.2 0 60 298 27 720.2 0 27 299 12 720.2 0 12 300 7 720.2 0 7 301 32 720.2 0 32 Finished in 9.80 s (69 us/read; 0.87 M reads/minute). === Summary === Total reads processed: 142,515 Reads with adapters: 49,340 (34.6%) Reads that were too short: 42 (0.0%) Reads written (passing filters): 49,298 (34.6%) Total basepairs processed: 37,886,251 bp Total written (filtered): 13,597,207 bp (35.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 49340 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 8 0.0 3 0 0 8 16 292 0.0 3 0 283 8 1 17 48319 0.0 3 47299 988 30 2 18 130 0.0 3 17 83 8 22 19 30 0.0 3 0 3 20 7 20 484 0.0 3 451 11 0 22 21 9 0.0 3 0 0 0 9 22 1 0.0 3 1 29 1 0.0 3 1 30 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 81 14 0.0 3 11 2 0 1 82 1 0.0 3 0 0 0 1 97 3 0.0 3 0 0 0 3 98 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 110 1 0.0 3 1 113 2 0.0 3 0 0 0 2 119 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 4 0.0 3 0 0 0 4 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.84 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 49,298 Reads with adapters: 443 (0.9%) Reads written (passing filters): 49,298 (100.0%) Total basepairs processed: 13,597,207 bp Total written (filtered): 13,595,501 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 443 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.7% C: 2.0% G: 79.5% T: 1.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 80 770.3 0 80 4 355 192.6 0 355 5 6 48.1 0 6 6 1 12.0 0 1 10 1 0.0 1 0 1