This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 136.10 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 172,358 (5.8%) Reads that were too short: 10,726 (0.4%) Reads written (passing filters): 161,632 (5.5%) Total basepairs processed: 887,907,860 bp Total written (filtered): 46,329,747 bp (5.2%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 172358 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1259 720.2 0 1259 7 83195 720.2 0 83195 8 66225 720.2 0 66225 9 54 720.2 0 54 10 8 720.2 0 8 11 14 720.2 0 14 12 13 720.2 0 13 13 8 720.2 0 8 14 13 720.2 0 13 15 18 720.2 0 18 16 13 720.2 0 13 17 9 720.2 0 9 18 39 720.2 0 39 19 19 720.2 0 19 20 49 720.2 0 49 21 16 720.2 0 16 22 10 720.2 0 10 23 12 720.2 0 12 24 3 720.2 0 3 25 6 720.2 0 6 26 12 720.2 0 12 27 35 720.2 0 35 28 20 720.2 0 20 29 27 720.2 0 27 30 15 720.2 0 15 31 30 720.2 0 30 32 21 720.2 0 21 33 17 720.2 0 17 34 21 720.2 0 21 35 40 720.2 0 40 36 109 720.2 0 109 37 374 720.2 0 374 38 289 720.2 0 289 39 28 720.2 0 28 40 15 720.2 0 15 41 18 720.2 0 18 42 17 720.2 0 17 43 70 720.2 0 70 44 682 720.2 0 682 45 879 720.2 0 879 46 14 720.2 0 14 47 11 720.2 0 11 48 6 720.2 0 6 49 24 720.2 0 24 50 12 720.2 0 12 51 15 720.2 0 15 52 20 720.2 0 20 53 11 720.2 0 11 54 14 720.2 0 14 55 21 720.2 0 21 56 26 720.2 0 26 57 32 720.2 0 32 58 18 720.2 0 18 59 24 720.2 0 24 60 8 720.2 0 8 61 17 720.2 0 17 62 28 720.2 0 28 63 11 720.2 0 11 64 4 720.2 0 4 65 12 720.2 0 12 66 15 720.2 0 15 67 12 720.2 0 12 68 20 720.2 0 20 69 13 720.2 0 13 70 17 720.2 0 17 71 35 720.2 0 35 72 24 720.2 0 24 73 70 720.2 0 70 74 13 720.2 0 13 75 23 720.2 0 23 76 11 720.2 0 11 77 13 720.2 0 13 78 25 720.2 0 25 79 26 720.2 0 26 80 21 720.2 0 21 81 21 720.2 0 21 82 21 720.2 0 21 83 25 720.2 0 25 84 35 720.2 0 35 85 35 720.2 0 35 86 8 720.2 0 8 87 19 720.2 0 19 88 19 720.2 0 19 89 23 720.2 0 23 90 4 720.2 0 4 91 22 720.2 0 22 92 14 720.2 0 14 93 20 720.2 0 20 94 28 720.2 0 28 95 17 720.2 0 17 96 52 720.2 0 52 97 17 720.2 0 17 98 8 720.2 0 8 99 16 720.2 0 16 100 11 720.2 0 11 101 17 720.2 0 17 102 13 720.2 0 13 103 18 720.2 0 18 104 13 720.2 0 13 105 16 720.2 0 16 106 15 720.2 0 15 107 25 720.2 0 25 108 19 720.2 0 19 109 22 720.2 0 22 110 14 720.2 0 14 111 13 720.2 0 13 112 26 720.2 0 26 113 24 720.2 0 24 114 36 720.2 0 36 115 28 720.2 0 28 116 18 720.2 0 18 117 15 720.2 0 15 118 23 720.2 0 23 119 27 720.2 0 27 120 31 720.2 0 31 121 36 720.2 0 36 122 73 720.2 0 73 123 27 720.2 0 27 124 29 720.2 0 29 125 36 720.2 0 36 126 114 720.2 0 114 127 187 720.2 0 187 128 284 720.2 0 284 129 108 720.2 0 108 130 79 720.2 0 79 131 93 720.2 0 93 132 213 720.2 0 213 133 82 720.2 0 82 134 66 720.2 0 66 135 467 720.2 0 467 136 187 720.2 0 187 137 552 720.2 0 552 138 31 720.2 0 31 139 89 720.2 0 89 140 110 720.2 0 110 141 120 720.2 0 120 142 125 720.2 0 125 143 104 720.2 0 104 144 150 720.2 0 150 145 102 720.2 0 102 146 34 720.2 0 34 147 67 720.2 0 67 148 441 720.2 0 441 149 336 720.2 0 336 150 279 720.2 0 279 151 35 720.2 0 35 152 22 720.2 0 22 153 23 720.2 0 23 154 13 720.2 0 13 155 22 720.2 0 22 156 30 720.2 0 30 157 33 720.2 0 33 158 26 720.2 0 26 159 28 720.2 0 28 160 44 720.2 0 44 161 58 720.2 0 58 162 28 720.2 0 28 163 30 720.2 0 30 164 26 720.2 0 26 165 37 720.2 0 37 166 38 720.2 0 38 167 59 720.2 0 59 168 35 720.2 0 35 169 94 720.2 0 94 170 24 720.2 0 24 171 18 720.2 0 18 172 27 720.2 0 27 173 20 720.2 0 20 174 29 720.2 0 29 175 51 720.2 0 51 176 17 720.2 0 17 177 29 720.2 0 29 178 20 720.2 0 20 179 34 720.2 0 34 180 38 720.2 0 38 181 30 720.2 0 30 182 64 720.2 0 64 183 42 720.2 0 42 184 40 720.2 0 40 185 50 720.2 0 50 186 41 720.2 0 41 187 28 720.2 0 28 188 32 720.2 0 32 189 32 720.2 0 32 190 47 720.2 0 47 191 108 720.2 0 108 192 38 720.2 0 38 193 26 720.2 0 26 194 45 720.2 0 45 195 30 720.2 0 30 196 45 720.2 0 45 197 28 720.2 0 28 198 15 720.2 0 15 199 23 720.2 0 23 200 29 720.2 0 29 201 34 720.2 0 34 202 28 720.2 0 28 203 30 720.2 0 30 204 30 720.2 0 30 205 19 720.2 0 19 206 25 720.2 0 25 207 73 720.2 0 73 208 100 720.2 0 100 209 172 720.2 0 172 210 102 720.2 0 102 211 294 720.2 0 294 212 57 720.2 0 57 213 53 720.2 0 53 214 184 720.2 0 184 215 122 720.2 0 122 216 196 720.2 0 196 217 45 720.2 0 45 218 29 720.2 0 29 219 44 720.2 0 44 220 42 720.2 0 42 221 56 720.2 0 56 222 58 720.2 0 58 223 37 720.2 0 37 224 54 720.2 0 54 225 117 720.2 0 117 226 68 720.2 0 68 227 51 720.2 0 51 228 59 720.2 0 59 229 56 720.2 0 56 230 123 720.2 0 123 231 49 720.2 0 49 232 53 720.2 0 53 233 86 720.2 0 86 234 115 720.2 0 115 235 152 720.2 0 152 236 67 720.2 0 67 237 38 720.2 0 38 238 38 720.2 0 38 239 28 720.2 0 28 240 40 720.2 0 40 241 36 720.2 0 36 242 51 720.2 0 51 243 60 720.2 0 60 244 56 720.2 0 56 245 50 720.2 0 50 246 47 720.2 0 47 247 27 720.2 0 27 248 38 720.2 0 38 249 34 720.2 0 34 250 24 720.2 0 24 251 32 720.2 0 32 252 41 720.2 0 41 253 48 720.2 0 48 254 65 720.2 0 65 255 47 720.2 0 47 256 51 720.2 0 51 257 43 720.2 0 43 258 60 720.2 0 60 259 54 720.2 0 54 260 90 720.2 0 90 261 81 720.2 0 81 262 56 720.2 0 56 263 41 720.2 0 41 264 59 720.2 0 59 265 49 720.2 0 49 266 63 720.2 0 63 267 59 720.2 0 59 268 93 720.2 0 93 269 285 720.2 0 285 270 246 720.2 0 246 271 331 720.2 0 331 272 98 720.2 0 98 273 125 720.2 0 125 274 107 720.2 0 107 275 104 720.2 0 104 276 105 720.2 0 105 277 66 720.2 0 66 278 50 720.2 0 50 279 75 720.2 0 75 280 96 720.2 0 96 281 131 720.2 0 131 282 101 720.2 0 101 283 78 720.2 0 78 284 63 720.2 0 63 285 88 720.2 0 88 286 65 720.2 0 65 287 45 720.2 0 45 288 74 720.2 0 74 289 81 720.2 0 81 290 104 720.2 0 104 291 102 720.2 0 102 292 99 720.2 0 99 293 148 720.2 0 148 294 465 720.2 0 465 295 864 720.2 0 864 296 1002 720.2 0 1002 297 338 720.2 0 338 298 126 720.2 0 126 299 87 720.2 0 87 300 138 720.2 0 138 301 394 720.2 0 394 Finished in 12.53 s (78 us/read; 0.77 M reads/minute). === Summary === Total reads processed: 161,632 Reads with adapters: 70,528 (43.6%) Reads that were too short: 47 (0.0%) Reads written (passing filters): 70,481 (43.6%) Total basepairs processed: 46,329,747 bp Total written (filtered): 18,991,547 bp (41.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 70528 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 8 0.0 3 0 0 0 5 3 18 24 0.0 3 0 0 17 7 19 908 0.0 3 0 744 82 59 23 20 66777 0.0 4 63172 2913 520 117 55 21 149 0.0 4 31 99 13 4 2 22 15 0.0 4 2 0 10 1 2 23 219 0.0 4 1 1 0 153 64 24 3 0.0 4 0 0 0 1 2 25 1 0.0 4 1 32 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 35 1 0.0 4 1 36 1 0.0 4 1 38 1 0.0 4 0 1 48 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 91 45 0.0 4 0 0 0 0 45 92 3 0.0 4 0 0 0 0 3 94 1 0.0 4 0 0 0 0 1 97 2 0.0 4 0 0 0 0 2 98 12 0.0 4 0 0 0 0 12 104 1 0.0 4 0 0 0 0 1 125 6 0.0 4 0 0 0 0 6 126 25 0.0 4 0 0 0 0 25 127 2262 0.0 4 0 0 0 0 2262 128 5 0.0 4 0 0 0 0 5 130 3 0.0 4 0 0 0 0 3 152 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 166 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 202 2 0.0 4 0 0 0 0 2 208 1 0.0 4 0 0 0 0 1 216 2 0.0 4 0 0 0 0 2 217 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 3 0.0 4 0 0 0 0 3 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 4 0.0 4 0 0 0 1 3 237 1 0.0 4 0 0 0 0 1 240 3 0.0 4 0 0 0 0 3 256 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 260 2 0.0 4 0 0 0 0 2 261 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 287 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 294 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.23 s (60 us/read; 1.00 M reads/minute). === Summary === Total reads processed: 70,481 Reads with adapters: 13,535 (19.2%) Reads written (passing filters): 70,481 (100.0%) Total basepairs processed: 18,991,547 bp Total written (filtered): 18,838,517 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 13535 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.8% C: 5.6% G: 90.7% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 109 1101.3 0 109 4 16 275.3 0 16 5 2 68.8 0 2 7 1 4.3 0 1 8 1 1.1 0 1 9 160 0.3 0 9 151 10 2521 0.1 1 131 2390 11 10571 0.0 1 196 10375 12 95 0.0 1 2 93 13 7 0.0 1 0 7 14 9 0.0 1 0 9 15 1 0.0 1 0 1 135 2 0.0 1 2 151 7 0.0 1 7 170 1 0.0 1 0 1 190 1 0.0 1 0 1 209 25 0.0 1 22 3 211 1 0.0 1 0 1 223 1 0.0 1 1 224 2 0.0 1 1 1 248 1 0.0 1 0 1 269 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.