This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 133.40 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 168,582 (5.7%) Reads that were too short: 5,907 (0.2%) Reads written (passing filters): 162,675 (5.5%) Total basepairs processed: 887,907,860 bp Total written (filtered): 46,961,747 bp (5.3%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 168582 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1019 720.2 0 1019 7 70414 720.2 0 70414 8 83980 720.2 0 83980 9 58 720.2 0 58 10 10 720.2 0 10 11 8 720.2 0 8 12 11 720.2 0 11 13 9 720.2 0 9 14 12 720.2 0 12 15 17 720.2 0 17 16 14 720.2 0 14 17 14 720.2 0 14 18 32 720.2 0 32 19 15 720.2 0 15 20 47 720.2 0 47 21 10 720.2 0 10 22 9 720.2 0 9 23 14 720.2 0 14 24 11 720.2 0 11 25 13 720.2 0 13 26 14 720.2 0 14 27 16 720.2 0 16 28 9 720.2 0 9 29 13 720.2 0 13 30 17 720.2 0 17 31 14 720.2 0 14 32 19 720.2 0 19 33 16 720.2 0 16 34 16 720.2 0 16 35 22 720.2 0 22 36 73 720.2 0 73 37 130 720.2 0 130 38 155 720.2 0 155 39 19 720.2 0 19 40 16 720.2 0 16 41 12 720.2 0 12 42 17 720.2 0 17 43 27 720.2 0 27 44 166 720.2 0 166 45 192 720.2 0 192 46 18 720.2 0 18 47 26 720.2 0 26 48 15 720.2 0 15 49 32 720.2 0 32 50 15 720.2 0 15 51 14 720.2 0 14 52 29 720.2 0 29 53 20 720.2 0 20 54 30 720.2 0 30 55 27 720.2 0 27 56 53 720.2 0 53 57 24 720.2 0 24 58 32 720.2 0 32 59 31 720.2 0 31 60 9 720.2 0 9 61 17 720.2 0 17 62 11 720.2 0 11 63 16 720.2 0 16 64 17 720.2 0 17 65 22 720.2 0 22 66 15 720.2 0 15 67 10 720.2 0 10 68 14 720.2 0 14 69 8 720.2 0 8 70 11 720.2 0 11 71 29 720.2 0 29 72 18 720.2 0 18 73 49 720.2 0 49 74 15 720.2 0 15 75 15 720.2 0 15 76 18 720.2 0 18 77 20 720.2 0 20 78 19 720.2 0 19 79 19 720.2 0 19 80 20 720.2 0 20 81 12 720.2 0 12 82 12 720.2 0 12 83 12 720.2 0 12 84 22 720.2 0 22 85 20 720.2 0 20 86 12 720.2 0 12 87 23 720.2 0 23 88 28 720.2 0 28 89 23 720.2 0 23 90 21 720.2 0 21 91 21 720.2 0 21 92 18 720.2 0 18 93 21 720.2 0 21 94 28 720.2 0 28 95 25 720.2 0 25 96 40 720.2 0 40 97 19 720.2 0 19 98 10 720.2 0 10 99 16 720.2 0 16 100 7 720.2 0 7 101 17 720.2 0 17 102 16 720.2 0 16 103 14 720.2 0 14 104 23 720.2 0 23 105 25 720.2 0 25 106 14 720.2 0 14 107 17 720.2 0 17 108 25 720.2 0 25 109 11 720.2 0 11 110 8 720.2 0 8 111 22 720.2 0 22 112 14 720.2 0 14 113 21 720.2 0 21 114 19 720.2 0 19 115 34 720.2 0 34 116 21 720.2 0 21 117 16 720.2 0 16 118 24 720.2 0 24 119 11 720.2 0 11 120 30 720.2 0 30 121 17 720.2 0 17 122 36 720.2 0 36 123 21 720.2 0 21 124 25 720.2 0 25 125 26 720.2 0 26 126 66 720.2 0 66 127 51 720.2 0 51 128 147 720.2 0 147 129 53 720.2 0 53 130 44 720.2 0 44 131 94 720.2 0 94 132 250 720.2 0 250 133 107 720.2 0 107 134 64 720.2 0 64 135 409 720.2 0 409 136 166 720.2 0 166 137 272 720.2 0 272 138 26 720.2 0 26 139 88 720.2 0 88 140 80 720.2 0 80 141 80 720.2 0 80 142 93 720.2 0 93 143 100 720.2 0 100 144 118 720.2 0 118 145 108 720.2 0 108 146 22 720.2 0 22 147 55 720.2 0 55 148 237 720.2 0 237 149 207 720.2 0 207 150 230 720.2 0 230 151 24 720.2 0 24 152 19 720.2 0 19 153 23 720.2 0 23 154 22 720.2 0 22 155 28 720.2 0 28 156 26 720.2 0 26 157 13 720.2 0 13 158 16 720.2 0 16 159 20 720.2 0 20 160 33 720.2 0 33 161 24 720.2 0 24 162 27 720.2 0 27 163 20 720.2 0 20 164 17 720.2 0 17 165 23 720.2 0 23 166 25 720.2 0 25 167 24 720.2 0 24 168 22 720.2 0 22 169 28 720.2 0 28 170 22 720.2 0 22 171 18 720.2 0 18 172 14 720.2 0 14 173 17 720.2 0 17 174 22 720.2 0 22 175 23 720.2 0 23 176 18 720.2 0 18 177 17 720.2 0 17 178 20 720.2 0 20 179 25 720.2 0 25 180 19 720.2 0 19 181 18 720.2 0 18 182 29 720.2 0 29 183 19 720.2 0 19 184 18 720.2 0 18 185 19 720.2 0 19 186 30 720.2 0 30 187 16 720.2 0 16 188 15 720.2 0 15 189 16 720.2 0 16 190 35 720.2 0 35 191 108 720.2 0 108 192 18 720.2 0 18 193 21 720.2 0 21 194 19 720.2 0 19 195 16 720.2 0 16 196 31 720.2 0 31 197 23 720.2 0 23 198 18 720.2 0 18 199 21 720.2 0 21 200 20 720.2 0 20 201 34 720.2 0 34 202 23 720.2 0 23 203 18 720.2 0 18 204 21 720.2 0 21 205 13 720.2 0 13 206 23 720.2 0 23 207 33 720.2 0 33 208 57 720.2 0 57 209 66 720.2 0 66 210 47 720.2 0 47 211 203 720.2 0 203 212 48 720.2 0 48 213 37 720.2 0 37 214 88 720.2 0 88 215 41 720.2 0 41 216 99 720.2 0 99 217 20 720.2 0 20 218 10 720.2 0 10 219 11 720.2 0 11 220 21 720.2 0 21 221 24 720.2 0 24 222 40 720.2 0 40 223 12 720.2 0 12 224 25 720.2 0 25 225 97 720.2 0 97 226 34 720.2 0 34 227 28 720.2 0 28 228 26 720.2 0 26 229 51 720.2 0 51 230 57 720.2 0 57 231 29 720.2 0 29 232 22 720.2 0 22 233 37 720.2 0 37 234 66 720.2 0 66 235 67 720.2 0 67 236 33 720.2 0 33 237 24 720.2 0 24 238 28 720.2 0 28 239 17 720.2 0 17 240 28 720.2 0 28 241 20 720.2 0 20 242 22 720.2 0 22 243 29 720.2 0 29 244 23 720.2 0 23 245 31 720.2 0 31 246 23 720.2 0 23 247 19 720.2 0 19 248 21 720.2 0 21 249 19 720.2 0 19 250 19 720.2 0 19 251 19 720.2 0 19 252 30 720.2 0 30 253 28 720.2 0 28 254 44 720.2 0 44 255 27 720.2 0 27 256 33 720.2 0 33 257 29 720.2 0 29 258 41 720.2 0 41 259 30 720.2 0 30 260 34 720.2 0 34 261 50 720.2 0 50 262 28 720.2 0 28 263 29 720.2 0 29 264 30 720.2 0 30 265 26 720.2 0 26 266 60 720.2 0 60 267 41 720.2 0 41 268 44 720.2 0 44 269 116 720.2 0 116 270 129 720.2 0 129 271 137 720.2 0 137 272 40 720.2 0 40 273 43 720.2 0 43 274 44 720.2 0 44 275 38 720.2 0 38 276 48 720.2 0 48 277 35 720.2 0 35 278 34 720.2 0 34 279 73 720.2 0 73 280 63 720.2 0 63 281 76 720.2 0 76 282 44 720.2 0 44 283 53 720.2 0 53 284 41 720.2 0 41 285 42 720.2 0 42 286 59 720.2 0 59 287 42 720.2 0 42 288 41 720.2 0 41 289 27 720.2 0 27 290 46 720.2 0 46 291 58 720.2 0 58 292 58 720.2 0 58 293 74 720.2 0 74 294 243 720.2 0 243 295 512 720.2 0 512 296 540 720.2 0 540 297 187 720.2 0 187 298 91 720.2 0 91 299 45 720.2 0 45 300 78 720.2 0 78 301 277 720.2 0 277 Finished in 11.62 s (71 us/read; 0.84 M reads/minute). === Summary === Total reads processed: 162,675 Reads with adapters: 69,284 (42.6%) Reads that were too short: 30 (0.0%) Reads written (passing filters): 69,254 (42.6%) Total basepairs processed: 46,961,747 bp Total written (filtered): 19,178,691 bp (40.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 69284 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 11 0.0 3 0 0 11 16 402 0.0 3 0 389 10 3 17 68560 0.0 3 67114 1392 47 7 18 161 0.0 3 13 142 6 19 32 0.0 3 4 0 24 4 20 50 0.0 3 0 0 1 49 27 1 0.0 3 1 32 2 0.0 3 0 0 0 2 36 1 0.0 3 1 43 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 0 2 48 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 3 0.0 3 0 0 0 3 60 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 1 79 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 99 1 0.0 3 1 106 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 125 3 0.0 3 0 0 0 3 127 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 152 1 0.0 3 1 153 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 182 1 0.0 3 0 0 0 1 184 3 0.0 3 0 0 0 3 192 1 0.0 3 0 0 0 1 193 1 0.0 3 1 204 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 3 0.0 3 0 0 0 3 281 4 0.0 3 0 0 1 3 282 4 0.0 3 0 0 0 4 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.07 s (59 us/read; 1.02 M reads/minute). === Summary === Total reads processed: 69,254 Reads with adapters: 113 (0.2%) Reads written (passing filters): 69,254 (100.0%) Total basepairs processed: 19,178,691 bp Total written (filtered): 19,178,338 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 113 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 45.1% C: 26.5% G: 20.4% T: 8.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 99 1082.1 0 99 4 14 270.5 0 14