This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 143.83 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 594,807 (20.2%) Reads that were too short: 74,517 (2.5%) Reads written (passing filters): 520,290 (17.6%) Total basepairs processed: 887,907,860 bp Total written (filtered): 146,247,120 bp (16.5%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 594807 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3810 720.2 0 3810 7 59386 720.2 0 59386 8 3158 720.2 0 3158 9 66559 720.2 0 66559 10 6249 720.2 0 6249 11 334435 720.2 0 334435 12 434 720.2 0 434 13 87 720.2 0 87 14 66 720.2 0 66 15 59 720.2 0 59 16 47 720.2 0 47 17 39 720.2 0 39 18 46 720.2 0 46 19 54 720.2 0 54 20 68 720.2 0 68 21 37 720.2 0 37 22 60 720.2 0 60 23 54 720.2 0 54 24 49 720.2 0 49 25 46 720.2 0 46 26 70 720.2 0 70 27 33 720.2 0 33 28 73 720.2 0 73 29 175 720.2 0 175 30 63 720.2 0 63 31 48 720.2 0 48 32 58 720.2 0 58 33 43 720.2 0 43 34 71 720.2 0 71 35 56 720.2 0 56 36 78 720.2 0 78 37 59 720.2 0 59 38 64 720.2 0 64 39 101 720.2 0 101 40 169 720.2 0 169 41 103 720.2 0 103 42 62 720.2 0 62 43 57 720.2 0 57 44 82 720.2 0 82 45 73 720.2 0 73 46 52 720.2 0 52 47 64 720.2 0 64 48 99 720.2 0 99 49 205 720.2 0 205 50 90 720.2 0 90 51 58 720.2 0 58 52 62 720.2 0 62 53 74 720.2 0 74 54 83 720.2 0 83 55 56 720.2 0 56 56 72 720.2 0 72 57 86 720.2 0 86 58 69 720.2 0 69 59 73 720.2 0 73 60 62 720.2 0 62 61 85 720.2 0 85 62 73 720.2 0 73 63 57 720.2 0 57 64 68 720.2 0 68 65 77 720.2 0 77 66 68 720.2 0 68 67 70 720.2 0 70 68 42 720.2 0 42 69 61 720.2 0 61 70 156 720.2 0 156 71 157 720.2 0 157 72 92 720.2 0 92 73 60 720.2 0 60 74 56 720.2 0 56 75 67 720.2 0 67 76 74 720.2 0 74 77 61 720.2 0 61 78 97 720.2 0 97 79 293 720.2 0 293 80 159 720.2 0 159 81 230 720.2 0 230 82 132 720.2 0 132 83 1254 720.2 0 1254 84 1498 720.2 0 1498 85 288 720.2 0 288 86 63 720.2 0 63 87 73 720.2 0 73 88 70 720.2 0 70 89 67 720.2 0 67 90 76 720.2 0 76 91 67 720.2 0 67 92 65 720.2 0 65 93 70 720.2 0 70 94 61 720.2 0 61 95 54 720.2 0 54 96 72 720.2 0 72 97 143 720.2 0 143 98 570 720.2 0 570 99 136 720.2 0 136 100 181 720.2 0 181 101 176 720.2 0 176 102 104 720.2 0 104 103 114 720.2 0 114 104 84 720.2 0 84 105 115 720.2 0 115 106 119 720.2 0 119 107 138 720.2 0 138 108 116 720.2 0 116 109 98 720.2 0 98 110 110 720.2 0 110 111 156 720.2 0 156 112 305 720.2 0 305 113 1853 720.2 0 1853 114 5701 720.2 0 5701 115 2996 720.2 0 2996 116 127 720.2 0 127 117 81 720.2 0 81 118 79 720.2 0 79 119 104 720.2 0 104 120 71 720.2 0 71 121 73 720.2 0 73 122 114 720.2 0 114 123 89 720.2 0 89 124 132 720.2 0 132 125 102 720.2 0 102 126 114 720.2 0 114 127 131 720.2 0 131 128 134 720.2 0 134 129 1730 720.2 0 1730 130 2634 720.2 0 2634 131 4911 720.2 0 4911 132 132 720.2 0 132 133 127 720.2 0 127 134 241 720.2 0 241 135 83 720.2 0 83 136 75 720.2 0 75 137 178 720.2 0 178 138 100 720.2 0 100 139 123 720.2 0 123 140 94 720.2 0 94 141 129 720.2 0 129 142 86 720.2 0 86 143 227 720.2 0 227 144 160 720.2 0 160 145 127 720.2 0 127 146 79 720.2 0 79 147 61 720.2 0 61 148 70 720.2 0 70 149 93 720.2 0 93 150 93 720.2 0 93 151 103 720.2 0 103 152 114 720.2 0 114 153 133 720.2 0 133 154 540 720.2 0 540 155 2557 720.2 0 2557 156 1413 720.2 0 1413 157 981 720.2 0 981 158 848 720.2 0 848 159 72 720.2 0 72 160 64 720.2 0 64 161 73 720.2 0 73 162 55 720.2 0 55 163 132 720.2 0 132 164 76 720.2 0 76 165 59 720.2 0 59 166 78 720.2 0 78 167 382 720.2 0 382 168 104 720.2 0 104 169 136 720.2 0 136 170 51 720.2 0 51 171 260 720.2 0 260 172 84 720.2 0 84 173 70 720.2 0 70 174 76 720.2 0 76 175 58 720.2 0 58 176 58 720.2 0 58 177 54 720.2 0 54 178 85 720.2 0 85 179 63 720.2 0 63 180 33 720.2 0 33 181 67 720.2 0 67 182 63 720.2 0 63 183 66 720.2 0 66 184 45 720.2 0 45 185 42 720.2 0 42 186 63 720.2 0 63 187 51 720.2 0 51 188 72 720.2 0 72 189 77 720.2 0 77 190 118 720.2 0 118 191 81 720.2 0 81 192 69 720.2 0 69 193 63 720.2 0 63 194 55 720.2 0 55 195 64 720.2 0 64 196 85 720.2 0 85 197 68 720.2 0 68 198 53 720.2 0 53 199 68 720.2 0 68 200 497 720.2 0 497 201 93 720.2 0 93 202 159 720.2 0 159 203 97 720.2 0 97 204 92 720.2 0 92 205 60 720.2 0 60 206 71 720.2 0 71 207 57 720.2 0 57 208 80 720.2 0 80 209 74 720.2 0 74 210 78 720.2 0 78 211 76 720.2 0 76 212 119 720.2 0 119 213 117 720.2 0 117 214 56 720.2 0 56 215 86 720.2 0 86 216 87 720.2 0 87 217 88 720.2 0 88 218 72 720.2 0 72 219 67 720.2 0 67 220 59 720.2 0 59 221 71 720.2 0 71 222 64 720.2 0 64 223 77 720.2 0 77 224 83 720.2 0 83 225 76 720.2 0 76 226 76 720.2 0 76 227 70 720.2 0 70 228 118 720.2 0 118 229 186 720.2 0 186 230 214 720.2 0 214 231 133 720.2 0 133 232 114 720.2 0 114 233 113 720.2 0 113 234 105 720.2 0 105 235 90 720.2 0 90 236 101 720.2 0 101 237 107 720.2 0 107 238 91 720.2 0 91 239 106 720.2 0 106 240 101 720.2 0 101 241 89 720.2 0 89 242 87 720.2 0 87 243 67 720.2 0 67 244 84 720.2 0 84 245 76 720.2 0 76 246 80 720.2 0 80 247 144 720.2 0 144 248 1058 720.2 0 1058 249 136 720.2 0 136 250 71 720.2 0 71 251 71 720.2 0 71 252 61 720.2 0 61 253 86 720.2 0 86 254 118 720.2 0 118 255 108 720.2 0 108 256 105 720.2 0 105 257 155 720.2 0 155 258 128 720.2 0 128 259 102 720.2 0 102 260 55 720.2 0 55 261 65 720.2 0 65 262 76 720.2 0 76 263 77 720.2 0 77 264 87 720.2 0 87 265 177 720.2 0 177 266 56 720.2 0 56 267 79 720.2 0 79 268 139 720.2 0 139 269 234 720.2 0 234 270 246 720.2 0 246 271 235 720.2 0 235 272 111 720.2 0 111 273 88 720.2 0 88 274 140 720.2 0 140 275 79 720.2 0 79 276 109 720.2 0 109 277 109 720.2 0 109 278 86 720.2 0 86 279 82 720.2 0 82 280 78 720.2 0 78 281 149 720.2 0 149 282 132 720.2 0 132 283 167 720.2 0 167 284 233 720.2 0 233 285 358 720.2 0 358 286 148 720.2 0 148 287 133 720.2 0 133 288 218 720.2 0 218 289 154 720.2 0 154 290 165 720.2 0 165 291 215 720.2 0 215 292 260 720.2 0 260 293 284 720.2 0 284 294 594 720.2 0 594 295 958 720.2 0 958 296 2237 720.2 0 2237 297 1544 720.2 0 1544 298 14667 720.2 0 14667 299 34131 720.2 0 34131 300 8723 720.2 0 8723 301 122 720.2 0 122 Finished in 33.94 s (65 us/read; 0.92 M reads/minute). === Summary === Total reads processed: 520,290 Reads with adapters: 56,865 (10.9%) Reads that were too short: 327 (0.1%) Reads written (passing filters): 56,538 (10.9%) Total basepairs processed: 146,247,120 bp Total written (filtered): 15,643,024 bp (10.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 56865 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 7 0.0 3 0 0 7 16 290 0.0 3 0 285 5 17 55912 0.0 3 54790 1085 33 4 18 103 0.0 3 11 89 3 19 23 0.0 3 2 0 20 1 20 71 0.0 3 0 0 0 71 22 1 0.0 3 0 0 0 1 23 4 0.0 3 0 0 0 4 25 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 32 37 0.0 3 0 0 0 37 34 4 0.0 3 0 0 0 4 35 6 0.0 3 0 0 0 6 37 1 0.0 3 0 0 1 38 7 0.0 3 0 0 0 7 40 1 0.0 3 0 0 0 1 46 4 0.0 3 0 0 1 3 47 1 0.0 3 1 50 3 0.0 3 0 0 0 3 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 62 5 0.0 3 0 0 0 5 65 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 11 0.0 3 0 0 11 76 2 0.0 3 0 0 0 2 77 4 0.0 3 0 0 0 4 88 1 0.0 3 0 0 0 1 89 1 0.0 3 1 94 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 1 119 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 147 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 1 154 4 0.0 3 0 0 0 4 157 1 0.0 3 0 0 0 1 162 6 0.0 3 0 0 0 6 163 2 0.0 3 0 0 0 2 164 4 0.0 3 0 0 1 3 168 2 0.0 3 0 0 0 2 178 2 0.0 3 0 0 0 2 180 1 0.0 3 0 0 0 1 182 1 0.0 3 1 184 2 0.0 3 0 0 0 2 186 2 0.0 3 0 0 0 2 190 12 0.0 3 0 0 0 12 192 5 0.0 3 0 0 1 4 194 1 0.0 3 0 0 0 1 200 29 0.0 3 0 0 0 29 203 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 214 5 0.0 3 0 0 0 5 215 176 0.0 3 0 0 4 172 216 2 0.0 3 0 0 0 2 217 5 0.0 3 0 0 0 5 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 220 2 0.0 3 0 0 0 2 221 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 230 1 0.0 3 1 235 1 0.0 3 0 0 1 236 10 0.0 3 0 0 0 10 238 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 268 2 0.0 3 0 0 0 2 269 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 277 3 0.0 3 0 0 0 3 278 11 0.0 3 0 0 0 11 279 14 0.0 3 0 0 0 14 280 8 0.0 3 0 0 0 8 281 2 0.0 3 0 0 0 2 282 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 286 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.09 s (55 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 56,538 Reads with adapters: 69 (0.1%) Reads written (passing filters): 56,538 (100.0%) Total basepairs processed: 15,643,024 bp Total written (filtered): 15,642,781 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 69 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 47.8% C: 15.9% G: 23.2% T: 13.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 56 883.4 0 56 4 4 220.9 0 4 5 7 55.2 0 7 12 2 0.0 1 0 2