This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GATATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 132.74 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 210,653 (7.1%) Reads that were too short: 13,205 (0.4%) Reads written (passing filters): 197,448 (6.7%) Total basepairs processed: 887,907,860 bp Total written (filtered): 55,439,160 bp (6.2%) === Adapter 1 === Sequence: GATATT; Type: regular 5'; Length: 6; Trimmed: 210653 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1286 720.2 0 1286 7 80769 720.2 0 80769 8 3190 720.2 0 3190 9 85477 720.2 0 85477 10 197 720.2 0 197 11 122 720.2 0 122 12 123 720.2 0 123 13 120 720.2 0 120 14 128 720.2 0 128 15 99 720.2 0 99 16 136 720.2 0 136 17 128 720.2 0 128 18 103 720.2 0 103 19 132 720.2 0 132 20 127 720.2 0 127 21 140 720.2 0 140 22 158 720.2 0 158 23 117 720.2 0 117 24 106 720.2 0 106 25 156 720.2 0 156 26 133 720.2 0 133 27 132 720.2 0 132 28 119 720.2 0 119 29 117 720.2 0 117 30 134 720.2 0 134 31 139 720.2 0 139 32 128 720.2 0 128 33 157 720.2 0 157 34 249 720.2 0 249 35 1332 720.2 0 1332 36 964 720.2 0 964 37 741 720.2 0 741 38 84 720.2 0 84 39 101 720.2 0 101 40 97 720.2 0 97 41 89 720.2 0 89 42 86 720.2 0 86 43 107 720.2 0 107 44 107 720.2 0 107 45 123 720.2 0 123 46 101 720.2 0 101 47 90 720.2 0 90 48 88 720.2 0 88 49 97 720.2 0 97 50 124 720.2 0 124 51 100 720.2 0 100 52 123 720.2 0 123 53 94 720.2 0 94 54 98 720.2 0 98 55 96 720.2 0 96 56 112 720.2 0 112 57 80 720.2 0 80 58 88 720.2 0 88 59 92 720.2 0 92 60 145 720.2 0 145 61 81 720.2 0 81 62 90 720.2 0 90 63 111 720.2 0 111 64 112 720.2 0 112 65 78 720.2 0 78 66 87 720.2 0 87 67 94 720.2 0 94 68 126 720.2 0 126 69 96 720.2 0 96 70 161 720.2 0 161 71 143 720.2 0 143 72 108 720.2 0 108 73 99 720.2 0 99 74 87 720.2 0 87 75 80 720.2 0 80 76 90 720.2 0 90 77 95 720.2 0 95 78 97 720.2 0 97 79 107 720.2 0 107 80 144 720.2 0 144 81 113 720.2 0 113 82 107 720.2 0 107 83 102 720.2 0 102 84 95 720.2 0 95 85 109 720.2 0 109 86 105 720.2 0 105 87 115 720.2 0 115 88 98 720.2 0 98 89 104 720.2 0 104 90 130 720.2 0 130 91 96 720.2 0 96 92 95 720.2 0 95 93 95 720.2 0 95 94 110 720.2 0 110 95 114 720.2 0 114 96 144 720.2 0 144 97 144 720.2 0 144 98 140 720.2 0 140 99 193 720.2 0 193 100 143 720.2 0 143 101 369 720.2 0 369 102 115 720.2 0 115 103 148 720.2 0 148 104 124 720.2 0 124 105 103 720.2 0 103 106 104 720.2 0 104 107 116 720.2 0 116 108 112 720.2 0 112 109 137 720.2 0 137 110 223 720.2 0 223 111 626 720.2 0 626 112 435 720.2 0 435 113 314 720.2 0 314 114 113 720.2 0 113 115 93 720.2 0 93 116 102 720.2 0 102 117 118 720.2 0 118 118 114 720.2 0 114 119 112 720.2 0 112 120 95 720.2 0 95 121 179 720.2 0 179 122 197 720.2 0 197 123 236 720.2 0 236 124 129 720.2 0 129 125 100 720.2 0 100 126 121 720.2 0 121 127 127 720.2 0 127 128 116 720.2 0 116 129 99 720.2 0 99 130 89 720.2 0 89 131 124 720.2 0 124 132 226 720.2 0 226 133 136 720.2 0 136 134 133 720.2 0 133 135 85 720.2 0 85 136 94 720.2 0 94 137 113 720.2 0 113 138 91 720.2 0 91 139 164 720.2 0 164 140 143 720.2 0 143 141 231 720.2 0 231 142 110 720.2 0 110 143 90 720.2 0 90 144 72 720.2 0 72 145 89 720.2 0 89 146 87 720.2 0 87 147 230 720.2 0 230 148 276 720.2 0 276 149 222 720.2 0 222 150 81 720.2 0 81 151 96 720.2 0 96 152 89 720.2 0 89 153 88 720.2 0 88 154 96 720.2 0 96 155 91 720.2 0 91 156 105 720.2 0 105 157 108 720.2 0 108 158 106 720.2 0 106 159 240 720.2 0 240 160 208 720.2 0 208 161 434 720.2 0 434 162 73 720.2 0 73 163 76 720.2 0 76 164 103 720.2 0 103 165 83 720.2 0 83 166 81 720.2 0 81 167 78 720.2 0 78 168 85 720.2 0 85 169 96 720.2 0 96 170 158 720.2 0 158 171 92 720.2 0 92 172 87 720.2 0 87 173 91 720.2 0 91 174 100 720.2 0 100 175 122 720.2 0 122 176 94 720.2 0 94 177 87 720.2 0 87 178 95 720.2 0 95 179 98 720.2 0 98 180 67 720.2 0 67 181 98 720.2 0 98 182 100 720.2 0 100 183 89 720.2 0 89 184 116 720.2 0 116 185 121 720.2 0 121 186 113 720.2 0 113 187 113 720.2 0 113 188 129 720.2 0 129 189 127 720.2 0 127 190 144 720.2 0 144 191 86 720.2 0 86 192 85 720.2 0 85 193 99 720.2 0 99 194 88 720.2 0 88 195 116 720.2 0 116 196 97 720.2 0 97 197 96 720.2 0 96 198 93 720.2 0 93 199 106 720.2 0 106 200 101 720.2 0 101 201 122 720.2 0 122 202 100 720.2 0 100 203 106 720.2 0 106 204 114 720.2 0 114 205 118 720.2 0 118 206 186 720.2 0 186 207 203 720.2 0 203 208 390 720.2 0 390 209 166 720.2 0 166 210 138 720.2 0 138 211 95 720.2 0 95 212 86 720.2 0 86 213 107 720.2 0 107 214 81 720.2 0 81 215 87 720.2 0 87 216 87 720.2 0 87 217 79 720.2 0 79 218 73 720.2 0 73 219 85 720.2 0 85 220 107 720.2 0 107 221 120 720.2 0 120 222 160 720.2 0 160 223 122 720.2 0 122 224 92 720.2 0 92 225 102 720.2 0 102 226 94 720.2 0 94 227 74 720.2 0 74 228 112 720.2 0 112 229 97 720.2 0 97 230 109 720.2 0 109 231 128 720.2 0 128 232 164 720.2 0 164 233 138 720.2 0 138 234 153 720.2 0 153 235 412 720.2 0 412 236 655 720.2 0 655 237 567 720.2 0 567 238 107 720.2 0 107 239 80 720.2 0 80 240 86 720.2 0 86 241 198 720.2 0 198 242 128 720.2 0 128 243 130 720.2 0 130 244 108 720.2 0 108 245 86 720.2 0 86 246 96 720.2 0 96 247 74 720.2 0 74 248 199 720.2 0 199 249 152 720.2 0 152 250 129 720.2 0 129 251 82 720.2 0 82 252 69 720.2 0 69 253 71 720.2 0 71 254 97 720.2 0 97 255 132 720.2 0 132 256 199 720.2 0 199 257 218 720.2 0 218 258 162 720.2 0 162 259 245 720.2 0 245 260 583 720.2 0 583 261 375 720.2 0 375 262 147 720.2 0 147 263 102 720.2 0 102 264 102 720.2 0 102 265 75 720.2 0 75 266 91 720.2 0 91 267 75 720.2 0 75 268 82 720.2 0 82 269 65 720.2 0 65 270 77 720.2 0 77 271 71 720.2 0 71 272 72 720.2 0 72 273 86 720.2 0 86 274 102 720.2 0 102 275 119 720.2 0 119 276 73 720.2 0 73 277 76 720.2 0 76 278 86 720.2 0 86 279 88 720.2 0 88 280 119 720.2 0 119 281 117 720.2 0 117 282 125 720.2 0 125 283 77 720.2 0 77 284 120 720.2 0 120 285 73 720.2 0 73 286 77 720.2 0 77 287 72 720.2 0 72 288 93 720.2 0 93 289 90 720.2 0 90 290 98 720.2 0 98 291 87 720.2 0 87 292 98 720.2 0 98 293 90 720.2 0 90 294 83 720.2 0 83 295 126 720.2 0 126 296 81 720.2 0 81 297 99 720.2 0 99 298 80 720.2 0 80 299 65 720.2 0 65 300 104 720.2 0 104 301 129 720.2 0 129 Finished in 14.60 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 197,448 Reads with adapters: 83,436 (42.3%) Reads that were too short: 60 (0.0%) Reads written (passing filters): 83,376 (42.2%) Total basepairs processed: 55,439,160 bp Total written (filtered): 22,562,299 bp (40.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 83436 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 3 18 36 0.0 3 0 0 29 4 3 19 857 0.0 3 0 723 86 31 17 20 79532 0.0 4 75634 3226 523 115 34 21 207 0.0 4 48 147 5 3 4 22 14 0.0 4 1 0 11 2 23 117 0.0 4 1 0 0 90 26 24 5 0.0 4 0 0 0 0 5 27 1 0.0 4 1 28 2 0.0 4 1 0 0 0 1 29 2 0.0 4 2 36 1 0.0 4 1 46 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 1 63 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 98 2 0.0 4 0 0 0 0 2 99 70 0.0 4 0 0 0 0 70 100 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 125 8 0.0 4 0 0 0 0 8 126 35 0.0 4 0 0 0 0 35 127 2459 0.0 4 0 0 0 0 2459 128 7 0.0 4 0 0 0 0 7 129 2 0.0 4 0 0 0 0 2 130 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 165 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 2 0.0 4 0 0 0 0 2 221 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 11 0.0 4 0 0 0 0 11 229 3 0.0 4 0 0 0 0 3 230 1 0.0 4 0 0 0 1 233 3 0.0 4 0 0 0 0 3 234 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 0 2 236 2 0.0 4 0 0 0 1 1 237 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 239 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 259 2 0.0 4 0 0 0 0 2 262 2 0.0 4 0 0 0 0 2 264 2 0.0 4 0 0 0 0 2 266 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 284 2 0.0 4 0 0 0 0 2 285 1 0.0 4 0 0 0 0 1 292 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.39 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 83,376 Reads with adapters: 3,367 (4.0%) Reads written (passing filters): 83,376 (100.0%) Total basepairs processed: 22,562,299 bp Total written (filtered): 22,494,880 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3367 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 12.1% C: 10.1% G: 75.9% T: 2.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 335 1302.8 0 335 4 37 325.7 0 37 5 26 81.4 0 26 6 1 20.4 0 1 7 1 5.1 0 1 8 3 1.3 0 3 9 23 0.3 0 1 22 10 61 0.1 1 3 58 11 1445 0.0 1 19 1426 12 1226 0.0 1 11 1215 13 30 0.0 1 1 29 15 4 0.0 1 0 4 16 2 0.0 1 0 2 25 1 0.0 1 1 44 1 0.0 1 1 60 1 0.0 1 1 149 1 0.0 1 1 152 15 0.0 1 10 5 158 1 0.0 1 0 1 160 1 0.0 1 0 1 171 1 0.0 1 1 179 16 0.0 1 7 9 180 1 0.0 1 1 191 2 0.0 1 0 2 199 1 0.0 1 1 201 1 0.0 1 1 210 124 0.0 1 109 15 211 5 0.0 1 5 225 1 0.0 1 1