This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 138.70 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 694,786 (23.6%) Reads that were too short: 10,317 (0.3%) Reads written (passing filters): 684,469 (23.2%) Total basepairs processed: 887,907,860 bp Total written (filtered): 191,632,687 bp (21.6%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 694786 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4475 720.2 0 4475 7 14252 720.2 0 14252 8 316116 720.2 0 316116 9 265413 720.2 0 265413 10 339 720.2 0 339 11 59 720.2 0 59 12 54 720.2 0 54 13 28 720.2 0 28 14 28 720.2 0 28 15 25 720.2 0 25 16 34 720.2 0 34 17 31 720.2 0 31 18 36 720.2 0 36 19 27 720.2 0 27 20 58 720.2 0 58 21 52 720.2 0 52 22 36 720.2 0 36 23 33 720.2 0 33 24 44 720.2 0 44 25 46 720.2 0 46 26 55 720.2 0 55 27 34 720.2 0 34 28 45 720.2 0 45 29 30 720.2 0 30 30 71 720.2 0 71 31 40 720.2 0 40 32 55 720.2 0 55 33 43 720.2 0 43 34 112 720.2 0 112 35 261 720.2 0 261 36 69 720.2 0 69 37 88 720.2 0 88 38 50 720.2 0 50 39 59 720.2 0 59 40 31 720.2 0 31 41 37 720.2 0 37 42 42 720.2 0 42 43 49 720.2 0 49 44 33 720.2 0 33 45 36 720.2 0 36 46 58 720.2 0 58 47 174 720.2 0 174 48 56 720.2 0 56 49 35 720.2 0 35 50 40 720.2 0 40 51 59 720.2 0 59 52 61 720.2 0 61 53 55 720.2 0 55 54 137 720.2 0 137 55 116 720.2 0 116 56 42 720.2 0 42 57 42 720.2 0 42 58 27 720.2 0 27 59 28 720.2 0 28 60 47 720.2 0 47 61 61 720.2 0 61 62 38 720.2 0 38 63 44 720.2 0 44 64 101 720.2 0 101 65 32 720.2 0 32 66 62 720.2 0 62 67 65 720.2 0 65 68 61 720.2 0 61 69 109 720.2 0 109 70 57 720.2 0 57 71 64 720.2 0 64 72 64 720.2 0 64 73 73 720.2 0 73 74 61 720.2 0 61 75 75 720.2 0 75 76 104 720.2 0 104 77 167 720.2 0 167 78 179 720.2 0 179 79 405 720.2 0 405 80 566 720.2 0 566 81 10830 720.2 0 10830 82 4978 720.2 0 4978 83 9537 720.2 0 9537 84 103 720.2 0 103 85 226 720.2 0 226 86 449 720.2 0 449 87 517 720.2 0 517 88 56 720.2 0 56 89 40 720.2 0 40 90 38 720.2 0 38 91 24 720.2 0 24 92 70 720.2 0 70 93 68 720.2 0 68 94 31 720.2 0 31 95 42 720.2 0 42 96 69 720.2 0 69 97 47 720.2 0 47 98 96 720.2 0 96 99 51 720.2 0 51 100 46 720.2 0 46 101 60 720.2 0 60 102 178 720.2 0 178 103 122 720.2 0 122 104 147 720.2 0 147 105 82 720.2 0 82 106 161 720.2 0 161 107 132 720.2 0 132 108 201 720.2 0 201 109 386 720.2 0 386 110 104 720.2 0 104 111 148 720.2 0 148 112 230 720.2 0 230 113 112 720.2 0 112 114 118 720.2 0 118 115 153 720.2 0 153 116 124 720.2 0 124 117 201 720.2 0 201 118 255 720.2 0 255 119 482 720.2 0 482 120 519 720.2 0 519 121 685 720.2 0 685 122 13077 720.2 0 13077 123 6641 720.2 0 6641 124 14549 720.2 0 14549 125 119 720.2 0 119 126 67 720.2 0 67 127 49 720.2 0 49 128 86 720.2 0 86 129 69 720.2 0 69 130 47 720.2 0 47 131 54 720.2 0 54 132 39 720.2 0 39 133 46 720.2 0 46 134 51 720.2 0 51 135 66 720.2 0 66 136 44 720.2 0 44 137 43 720.2 0 43 138 57 720.2 0 57 139 69 720.2 0 69 140 62 720.2 0 62 141 71 720.2 0 71 142 596 720.2 0 596 143 708 720.2 0 708 144 1065 720.2 0 1065 145 242 720.2 0 242 146 364 720.2 0 364 147 156 720.2 0 156 148 90 720.2 0 90 149 151 720.2 0 151 150 117 720.2 0 117 151 208 720.2 0 208 152 85 720.2 0 85 153 86 720.2 0 86 154 114 720.2 0 114 155 82 720.2 0 82 156 42 720.2 0 42 157 29 720.2 0 29 158 68 720.2 0 68 159 82 720.2 0 82 160 76 720.2 0 76 161 31 720.2 0 31 162 35 720.2 0 35 163 39 720.2 0 39 164 45 720.2 0 45 165 44 720.2 0 44 166 162 720.2 0 162 167 64 720.2 0 64 168 44 720.2 0 44 169 88 720.2 0 88 170 56 720.2 0 56 171 121 720.2 0 121 172 40 720.2 0 40 173 56 720.2 0 56 174 37 720.2 0 37 175 54 720.2 0 54 176 39 720.2 0 39 177 44 720.2 0 44 178 71 720.2 0 71 179 47 720.2 0 47 180 115 720.2 0 115 181 156 720.2 0 156 182 2109 720.2 0 2109 183 1081 720.2 0 1081 184 1495 720.2 0 1495 185 66 720.2 0 66 186 61 720.2 0 61 187 51 720.2 0 51 188 27 720.2 0 27 189 44 720.2 0 44 190 45 720.2 0 45 191 22 720.2 0 22 192 39 720.2 0 39 193 55 720.2 0 55 194 126 720.2 0 126 195 79 720.2 0 79 196 102 720.2 0 102 197 31 720.2 0 31 198 93 720.2 0 93 199 63 720.2 0 63 200 75 720.2 0 75 201 41 720.2 0 41 202 32 720.2 0 32 203 29 720.2 0 29 204 22 720.2 0 22 205 37 720.2 0 37 206 47 720.2 0 47 207 57 720.2 0 57 208 30 720.2 0 30 209 32 720.2 0 32 210 34 720.2 0 34 211 43 720.2 0 43 212 43 720.2 0 43 213 45 720.2 0 45 214 30 720.2 0 30 215 36 720.2 0 36 216 20 720.2 0 20 217 17 720.2 0 17 218 34 720.2 0 34 219 54 720.2 0 54 220 61 720.2 0 61 221 40 720.2 0 40 222 38 720.2 0 38 223 32 720.2 0 32 224 27 720.2 0 27 225 32 720.2 0 32 226 41 720.2 0 41 227 72 720.2 0 72 228 68 720.2 0 68 229 60 720.2 0 60 230 49 720.2 0 49 231 57 720.2 0 57 232 36 720.2 0 36 233 32 720.2 0 32 234 36 720.2 0 36 235 33 720.2 0 33 236 46 720.2 0 46 237 42 720.2 0 42 238 44 720.2 0 44 239 51 720.2 0 51 240 266 720.2 0 266 241 113 720.2 0 113 242 129 720.2 0 129 243 38 720.2 0 38 244 50 720.2 0 50 245 54 720.2 0 54 246 55 720.2 0 55 247 52 720.2 0 52 248 30 720.2 0 30 249 41 720.2 0 41 250 113 720.2 0 113 251 28 720.2 0 28 252 67 720.2 0 67 253 66 720.2 0 66 254 58 720.2 0 58 255 69 720.2 0 69 256 55 720.2 0 55 257 47 720.2 0 47 258 58 720.2 0 58 259 56 720.2 0 56 260 52 720.2 0 52 261 88 720.2 0 88 262 72 720.2 0 72 263 150 720.2 0 150 264 126 720.2 0 126 265 69 720.2 0 69 266 761 720.2 0 761 267 478 720.2 0 478 268 879 720.2 0 879 269 156 720.2 0 156 270 129 720.2 0 129 271 54 720.2 0 54 272 43 720.2 0 43 273 45 720.2 0 45 274 354 720.2 0 354 275 236 720.2 0 236 276 284 720.2 0 284 277 99 720.2 0 99 278 56 720.2 0 56 279 115 720.2 0 115 280 105 720.2 0 105 281 141 720.2 0 141 282 85 720.2 0 85 283 97 720.2 0 97 284 33 720.2 0 33 285 46 720.2 0 46 286 97 720.2 0 97 287 179 720.2 0 179 288 383 720.2 0 383 289 222 720.2 0 222 290 183 720.2 0 183 291 75 720.2 0 75 292 126 720.2 0 126 293 118 720.2 0 118 294 311 720.2 0 311 295 135 720.2 0 135 296 281 720.2 0 281 297 108 720.2 0 108 298 112 720.2 0 112 299 77 720.2 0 77 300 85 720.2 0 85 301 88 720.2 0 88 Finished in 47.91 s (70 us/read; 0.86 M reads/minute). === Summary === Total reads processed: 684,469 Reads with adapters: 280,535 (41.0%) Reads that were too short: 188 (0.0%) Reads written (passing filters): 280,347 (41.0%) Total basepairs processed: 191,632,687 bp Total written (filtered): 76,117,119 bp (39.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 280535 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 0 0 0 2 17 24 0.0 3 0 0 0 18 6 18 168 0.0 3 0 0 127 24 17 19 3939 0.0 3 0 3541 225 130 43 20 270061 0.0 4 257686 10133 1685 428 129 21 521 0.0 4 69 398 31 13 10 22 3672 0.0 4 3457 149 43 13 10 23 466 0.0 4 2 7 0 325 132 24 8 0.0 4 2 0 0 1 5 25 12 0.0 4 0 0 0 7 5 28 3 0.0 4 0 0 0 0 3 29 1 0.0 4 1 30 1 0.0 4 0 0 0 0 1 31 1 0.0 4 1 32 1 0.0 4 0 1 34 2 0.0 4 1 0 0 0 1 35 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 37 1 0.0 4 1 47 4 0.0 4 3 1 49 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 64 2 0.0 4 0 0 0 0 2 75 1 0.0 4 0 0 0 0 1 81 1 0.0 4 1 87 2 0.0 4 0 0 0 0 2 88 1 0.0 4 0 0 0 0 1 90 2 0.0 4 0 0 0 1 1 91 2 0.0 4 0 0 0 0 2 92 2 0.0 4 0 0 0 0 2 93 3 0.0 4 0 0 0 0 3 94 1 0.0 4 0 0 0 0 1 97 1 0.0 4 1 99 4 0.0 4 0 0 0 0 4 100 1 0.0 4 0 0 1 101 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 125 6 0.0 4 0 0 0 0 6 126 17 0.0 4 0 0 0 0 17 127 1367 0.0 4 0 0 0 0 1367 128 10 0.0 4 0 0 0 0 10 129 4 0.0 4 0 0 0 0 4 130 5 0.0 4 0 0 0 0 5 135 1 0.0 4 0 0 0 0 1 136 7 0.0 4 0 0 0 0 7 137 2 0.0 4 0 0 0 1 1 140 3 0.0 4 0 0 0 0 3 142 1 0.0 4 0 0 0 0 1 148 2 0.0 4 0 0 0 0 2 149 2 0.0 4 0 0 0 0 2 150 7 0.0 4 0 0 0 0 7 152 1 0.0 4 0 0 0 0 1 153 9 0.0 4 0 0 0 0 9 154 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 1 178 1 0.0 4 0 0 0 0 1 179 4 0.0 4 0 0 0 0 4 182 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 189 2 0.0 4 0 0 0 0 2 190 2 0.0 4 0 1 0 0 1 194 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 4 0.0 4 0 0 0 0 4 213 3 0.0 4 0 0 0 0 3 215 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 221 5 0.0 4 0 0 0 0 5 222 4 0.0 4 0 0 0 0 4 223 1 0.0 4 0 0 0 0 1 225 5 0.0 4 0 0 0 0 5 227 2 0.0 4 0 0 0 0 2 228 43 0.0 4 0 0 0 2 41 229 18 0.0 4 0 0 0 0 18 230 3 0.0 4 0 0 0 0 3 231 1 0.0 4 0 0 0 0 1 233 4 0.0 4 0 0 0 0 4 234 1 0.0 4 0 0 0 0 1 235 10 0.0 4 0 0 0 2 8 236 3 0.0 4 0 0 0 0 3 237 1 0.0 4 0 0 0 0 1 238 3 0.0 4 0 0 0 0 3 239 1 0.0 4 0 0 0 0 1 243 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 248 1 0.0 4 0 1 252 1 0.0 4 0 0 0 0 1 257 2 0.0 4 0 0 0 0 2 258 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 1 265 2 0.0 4 0 0 0 0 2 266 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 269 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 282 3 0.0 4 0 0 0 0 3 283 2 0.0 4 0 0 0 0 2 284 2 0.0 4 0 0 0 0 2 288 3 0.0 4 0 0 0 0 3 290 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 293 4 0.0 4 0 0 0 0 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 15.86 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 280,347 Reads with adapters: 56,426 (20.1%) Reads written (passing filters): 280,347 (100.0%) Total basepairs processed: 76,117,119 bp Total written (filtered): 75,497,666 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 56426 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.8% C: 6.1% G: 90.3% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 206 4380.4 0 206 4 73 1095.1 0 73 5 7 273.8 0 7 6 27 68.4 0 27 7 22 17.1 0 22 8 21 4.3 0 21 9 11030 1.1 0 683 10347 10 33152 0.3 1 1712 31440 11 11364 0.1 1 228 11136 12 111 0.0 1 2 109 13 86 0.0 1 1 85 14 14 0.0 1 0 14 15 1 0.0 1 0 1 22 2 0.0 1 2 26 2 0.0 1 1 1 28 1 0.0 1 1 32 1 0.0 1 1 40 2 0.0 1 1 1 50 1 0.0 1 1 55 1 0.0 1 1 68 2 0.0 1 2 74 1 0.0 1 0 1 88 1 0.0 1 0 1 91 2 0.0 1 2 107 1 0.0 1 1 114 1 0.0 1 1 121 3 0.0 1 3 122 1 0.0 1 1 147 3 0.0 1 3 149 1 0.0 1 1 150 14 0.0 1 7 7 152 2 0.0 1 0 2 153 1 0.0 1 1 155 3 0.0 1 3 160 1 0.0 1 1 161 2 0.0 1 2 167 1 0.0 1 1 169 18 0.0 1 16 2 175 1 0.0 1 1 189 4 0.0 1 1 3 191 1 0.0 1 1 199 1 0.0 1 1 205 1 0.0 1 1 207 3 0.0 1 2 1 208 215 0.0 1 175 40 209 6 0.0 1 6 210 4 0.0 1 4 211 5 0.0 1 4 1 220 1 0.0 1 0 1 232 1 0.0 1 1 271 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.