This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 140.77 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 733,293 (24.9%) Reads that were too short: 9,528 (0.3%) Reads written (passing filters): 723,765 (24.5%) Total basepairs processed: 887,907,860 bp Total written (filtered): 202,656,851 bp (22.8%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 733293 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3360 720.2 0 3360 7 9969 720.2 0 9969 8 286265 720.2 0 286265 9 334583 720.2 0 334583 10 359 720.2 0 359 11 60 720.2 0 60 12 44 720.2 0 44 13 53 720.2 0 53 14 30 720.2 0 30 15 27 720.2 0 27 16 37 720.2 0 37 17 28 720.2 0 28 18 43 720.2 0 43 19 37 720.2 0 37 20 28 720.2 0 28 21 42 720.2 0 42 22 47 720.2 0 47 23 44 720.2 0 44 24 34 720.2 0 34 25 29 720.2 0 29 26 32 720.2 0 32 27 43 720.2 0 43 28 35 720.2 0 35 29 35 720.2 0 35 30 58 720.2 0 58 31 27 720.2 0 27 32 34 720.2 0 34 33 46 720.2 0 46 34 63 720.2 0 63 35 57 720.2 0 57 36 54 720.2 0 54 37 87 720.2 0 87 38 54 720.2 0 54 39 66 720.2 0 66 40 39 720.2 0 39 41 41 720.2 0 41 42 49 720.2 0 49 43 44 720.2 0 44 44 53 720.2 0 53 45 34 720.2 0 34 46 49 720.2 0 49 47 197 720.2 0 197 48 77 720.2 0 77 49 64 720.2 0 64 50 48 720.2 0 48 51 40 720.2 0 40 52 50 720.2 0 50 53 78 720.2 0 78 54 128 720.2 0 128 55 79 720.2 0 79 56 47 720.2 0 47 57 35 720.2 0 35 58 38 720.2 0 38 59 35 720.2 0 35 60 46 720.2 0 46 61 57 720.2 0 57 62 40 720.2 0 40 63 70 720.2 0 70 64 124 720.2 0 124 65 47 720.2 0 47 66 72 720.2 0 72 67 79 720.2 0 79 68 85 720.2 0 85 69 147 720.2 0 147 70 57 720.2 0 57 71 77 720.2 0 77 72 74 720.2 0 74 73 53 720.2 0 53 74 93 720.2 0 93 75 108 720.2 0 108 76 123 720.2 0 123 77 177 720.2 0 177 78 214 720.2 0 214 79 419 720.2 0 419 80 594 720.2 0 594 81 13568 720.2 0 13568 82 6155 720.2 0 6155 83 12256 720.2 0 12256 84 101 720.2 0 101 85 139 720.2 0 139 86 129 720.2 0 129 87 160 720.2 0 160 88 53 720.2 0 53 89 52 720.2 0 52 90 44 720.2 0 44 91 48 720.2 0 48 92 81 720.2 0 81 93 76 720.2 0 76 94 59 720.2 0 59 95 49 720.2 0 49 96 67 720.2 0 67 97 48 720.2 0 48 98 106 720.2 0 106 99 53 720.2 0 53 100 47 720.2 0 47 101 65 720.2 0 65 102 116 720.2 0 116 103 103 720.2 0 103 104 135 720.2 0 135 105 116 720.2 0 116 106 142 720.2 0 142 107 155 720.2 0 155 108 209 720.2 0 209 109 379 720.2 0 379 110 111 720.2 0 111 111 144 720.2 0 144 112 202 720.2 0 202 113 109 720.2 0 109 114 121 720.2 0 121 115 213 720.2 0 213 116 164 720.2 0 164 117 181 720.2 0 181 118 189 720.2 0 189 119 339 720.2 0 339 120 391 720.2 0 391 121 496 720.2 0 496 122 13245 720.2 0 13245 123 6133 720.2 0 6133 124 14316 720.2 0 14316 125 106 720.2 0 106 126 61 720.2 0 61 127 56 720.2 0 56 128 83 720.2 0 83 129 63 720.2 0 63 130 63 720.2 0 63 131 66 720.2 0 66 132 41 720.2 0 41 133 53 720.2 0 53 134 56 720.2 0 56 135 56 720.2 0 56 136 41 720.2 0 41 137 51 720.2 0 51 138 56 720.2 0 56 139 63 720.2 0 63 140 64 720.2 0 64 141 70 720.2 0 70 142 592 720.2 0 592 143 775 720.2 0 775 144 1123 720.2 0 1123 145 185 720.2 0 185 146 261 720.2 0 261 147 123 720.2 0 123 148 73 720.2 0 73 149 102 720.2 0 102 150 63 720.2 0 63 151 107 720.2 0 107 152 57 720.2 0 57 153 52 720.2 0 52 154 62 720.2 0 62 155 68 720.2 0 68 156 45 720.2 0 45 157 36 720.2 0 36 158 51 720.2 0 51 159 60 720.2 0 60 160 84 720.2 0 84 161 38 720.2 0 38 162 51 720.2 0 51 163 52 720.2 0 52 164 57 720.2 0 57 165 51 720.2 0 51 166 121 720.2 0 121 167 60 720.2 0 60 168 75 720.2 0 75 169 99 720.2 0 99 170 57 720.2 0 57 171 123 720.2 0 123 172 64 720.2 0 64 173 54 720.2 0 54 174 49 720.2 0 49 175 51 720.2 0 51 176 49 720.2 0 49 177 49 720.2 0 49 178 79 720.2 0 79 179 74 720.2 0 74 180 119 720.2 0 119 181 142 720.2 0 142 182 2545 720.2 0 2545 183 1246 720.2 0 1246 184 1705 720.2 0 1705 185 58 720.2 0 58 186 61 720.2 0 61 187 57 720.2 0 57 188 46 720.2 0 46 189 45 720.2 0 45 190 47 720.2 0 47 191 29 720.2 0 29 192 42 720.2 0 42 193 44 720.2 0 44 194 62 720.2 0 62 195 67 720.2 0 67 196 73 720.2 0 73 197 30 720.2 0 30 198 75 720.2 0 75 199 70 720.2 0 70 200 64 720.2 0 64 201 61 720.2 0 61 202 46 720.2 0 46 203 44 720.2 0 44 204 39 720.2 0 39 205 43 720.2 0 43 206 44 720.2 0 44 207 85 720.2 0 85 208 32 720.2 0 32 209 51 720.2 0 51 210 42 720.2 0 42 211 33 720.2 0 33 212 56 720.2 0 56 213 51 720.2 0 51 214 42 720.2 0 42 215 43 720.2 0 43 216 26 720.2 0 26 217 32 720.2 0 32 218 40 720.2 0 40 219 68 720.2 0 68 220 53 720.2 0 53 221 48 720.2 0 48 222 46 720.2 0 46 223 38 720.2 0 38 224 34 720.2 0 34 225 41 720.2 0 41 226 51 720.2 0 51 227 55 720.2 0 55 228 46 720.2 0 46 229 68 720.2 0 68 230 57 720.2 0 57 231 26 720.2 0 26 232 31 720.2 0 31 233 48 720.2 0 48 234 44 720.2 0 44 235 37 720.2 0 37 236 68 720.2 0 68 237 64 720.2 0 64 238 53 720.2 0 53 239 63 720.2 0 63 240 212 720.2 0 212 241 114 720.2 0 114 242 107 720.2 0 107 243 45 720.2 0 45 244 38 720.2 0 38 245 62 720.2 0 62 246 35 720.2 0 35 247 52 720.2 0 52 248 44 720.2 0 44 249 45 720.2 0 45 250 103 720.2 0 103 251 38 720.2 0 38 252 73 720.2 0 73 253 80 720.2 0 80 254 52 720.2 0 52 255 61 720.2 0 61 256 74 720.2 0 74 257 57 720.2 0 57 258 71 720.2 0 71 259 54 720.2 0 54 260 50 720.2 0 50 261 61 720.2 0 61 262 74 720.2 0 74 263 125 720.2 0 125 264 135 720.2 0 135 265 70 720.2 0 70 266 796 720.2 0 796 267 507 720.2 0 507 268 907 720.2 0 907 269 113 720.2 0 113 270 93 720.2 0 93 271 48 720.2 0 48 272 53 720.2 0 53 273 48 720.2 0 48 274 269 720.2 0 269 275 205 720.2 0 205 276 240 720.2 0 240 277 58 720.2 0 58 278 53 720.2 0 53 279 100 720.2 0 100 280 63 720.2 0 63 281 106 720.2 0 106 282 74 720.2 0 74 283 98 720.2 0 98 284 64 720.2 0 64 285 49 720.2 0 49 286 94 720.2 0 94 287 134 720.2 0 134 288 177 720.2 0 177 289 117 720.2 0 117 290 111 720.2 0 111 291 52 720.2 0 52 292 97 720.2 0 97 293 110 720.2 0 110 294 290 720.2 0 290 295 97 720.2 0 97 296 191 720.2 0 191 297 92 720.2 0 92 298 108 720.2 0 108 299 60 720.2 0 60 300 64 720.2 0 64 301 70 720.2 0 70 Finished in 47.49 s (66 us/read; 0.91 M reads/minute). === Summary === Total reads processed: 723,765 Reads with adapters: 287,076 (39.7%) Reads that were too short: 384 (0.1%) Reads written (passing filters): 286,692 (39.6%) Total basepairs processed: 202,656,851 bp Total written (filtered): 79,058,717 bp (39.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 287076 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 6 0.0 2 0 0 0 6 15 48 0.0 3 0 0 45 3 16 1313 0.0 3 0 1279 33 1 17 283901 0.0 3 278447 5292 141 21 18 656 0.0 3 30 606 18 2 19 82 0.0 3 2 0 71 9 20 196 0.0 3 3 0 0 193 21 2 0.0 3 2 22 9 0.0 3 1 0 1 7 24 2 0.0 3 0 0 0 2 25 15 0.0 3 2 0 11 2 26 1 0.0 3 1 27 1 0.0 3 1 28 12 0.0 3 1 0 0 11 30 2 0.0 3 1 1 32 1 0.0 3 1 34 135 0.0 3 1 0 1 133 36 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 49 26 0.0 3 0 0 0 26 50 197 0.0 3 0 0 2 195 51 8 0.0 3 0 0 0 8 52 6 0.0 3 0 0 0 6 53 31 0.0 3 0 0 0 31 54 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 5 0.0 3 0 0 0 5 77 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 101 3 0.0 3 0 0 0 3 102 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 113 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 1 124 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 156 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 161 3 0.0 3 0 0 0 3 162 29 0.0 3 0 0 0 29 163 16 0.0 3 0 0 0 16 164 10 0.0 3 0 0 0 10 165 46 0.0 3 0 0 0 46 166 5 0.0 3 0 0 0 5 170 1 0.0 3 0 0 0 1 172 1 0.0 3 1 179 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 0 2 192 12 0.0 3 0 0 0 12 194 4 0.0 3 0 0 1 3 195 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 5 0.0 3 0 0 0 5 217 175 0.0 3 0 0 4 171 218 2 0.0 3 0 0 0 2 219 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 2 0.0 3 0 0 0 2 227 1 0.0 3 0 0 0 1 232 1 0.0 3 1 237 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 271 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 11 0.0 3 0 0 0 11 281 12 0.0 3 0 0 0 12 282 6 0.0 3 0 0 0 6 283 1 0.0 3 0 0 0 1 284 3 0.0 3 0 0 0 3 288 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 292 4 0.0 3 0 0 0 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 15.41 s (54 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 286,692 Reads with adapters: 1,309 (0.5%) Reads written (passing filters): 286,692 (100.0%) Total basepairs processed: 79,058,717 bp Total written (filtered): 79,054,355 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1309 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 65.2% C: 4.8% G: 26.5% T: 3.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 891 4479.6 0 891 4 402 1119.9 0 402 5 15 280.0 0 15 6 1 70.0 0 1