This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.41 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 470,291 (21.0%) Reads that were too short: 302,213 (13.5%) Reads written (passing filters): 168,078 (7.5%) Total basepairs processed: 673,916,425 bp Total written (filtered): 43,914,233 bp (6.5%) === Adapter 1 === Sequence: GTCCGG; Type: regular 5'; Length: 6; Trimmed: 470291 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 6713 546.6 0 6713 7 64916 546.6 0 64916 8 1389 546.6 0 1389 9 61020 546.6 0 61020 10 100 546.6 0 100 11 68 546.6 0 68 12 32 546.6 0 32 13 38 546.6 0 38 14 12 546.6 0 12 15 18 546.6 0 18 16 27 546.6 0 27 17 18 546.6 0 18 18 39 546.6 0 39 19 27 546.6 0 27 20 24 546.6 0 24 21 23 546.6 0 23 22 23 546.6 0 23 23 17 546.6 0 17 24 15 546.6 0 15 25 17 546.6 0 17 26 22 546.6 0 22 27 16 546.6 0 16 28 9 546.6 0 9 29 29 546.6 0 29 30 25 546.6 0 25 31 58 546.6 0 58 32 94 546.6 0 94 33 38 546.6 0 38 34 67 546.6 0 67 35 79 546.6 0 79 36 23 546.6 0 23 37 30 546.6 0 30 38 25 546.6 0 25 39 37 546.6 0 37 40 20 546.6 0 20 41 39 546.6 0 39 42 25 546.6 0 25 43 40 546.6 0 40 44 27 546.6 0 27 45 28 546.6 0 28 46 20 546.6 0 20 47 27 546.6 0 27 48 24 546.6 0 24 49 24 546.6 0 24 50 30 546.6 0 30 51 17 546.6 0 17 52 24 546.6 0 24 53 35 546.6 0 35 54 36 546.6 0 36 55 31 546.6 0 31 56 21 546.6 0 21 57 38 546.6 0 38 58 21 546.6 0 21 59 32 546.6 0 32 60 308 546.6 0 308 61 22 546.6 0 22 62 20 546.6 0 20 63 31 546.6 0 31 64 19 546.6 0 19 65 48 546.6 0 48 66 22 546.6 0 22 67 24 546.6 0 24 68 21 546.6 0 21 69 20 546.6 0 20 70 24 546.6 0 24 71 28 546.6 0 28 72 28 546.6 0 28 73 40 546.6 0 40 74 20 546.6 0 20 75 49 546.6 0 49 76 36 546.6 0 36 77 90 546.6 0 90 78 33 546.6 0 33 79 25 546.6 0 25 80 19 546.6 0 19 81 23 546.6 0 23 82 20 546.6 0 20 83 17 546.6 0 17 84 20 546.6 0 20 85 27 546.6 0 27 86 28 546.6 0 28 87 20 546.6 0 20 88 22 546.6 0 22 89 33 546.6 0 33 90 26 546.6 0 26 91 29 546.6 0 29 92 37 546.6 0 37 93 28 546.6 0 28 94 30 546.6 0 30 95 34 546.6 0 34 96 28 546.6 0 28 97 33 546.6 0 33 98 23 546.6 0 23 99 27 546.6 0 27 100 46 546.6 0 46 101 38 546.6 0 38 102 38 546.6 0 38 103 60 546.6 0 60 104 41 546.6 0 41 105 32 546.6 0 32 106 47 546.6 0 47 107 37 546.6 0 37 108 36 546.6 0 36 109 31 546.6 0 31 110 36 546.6 0 36 111 41 546.6 0 41 112 64 546.6 0 64 113 455 546.6 0 455 114 810 546.6 0 810 115 568 546.6 0 568 116 46 546.6 0 46 117 30 546.6 0 30 118 32 546.6 0 32 119 28 546.6 0 28 120 36 546.6 0 36 121 39 546.6 0 39 122 53 546.6 0 53 123 50 546.6 0 50 124 40 546.6 0 40 125 50 546.6 0 50 126 44 546.6 0 44 127 37 546.6 0 37 128 47 546.6 0 47 129 101 546.6 0 101 130 46 546.6 0 46 131 24 546.6 0 24 132 50 546.6 0 50 133 61 546.6 0 61 134 87 546.6 0 87 135 68 546.6 0 68 136 71 546.6 0 71 137 82 546.6 0 82 138 57 546.6 0 57 139 43 546.6 0 43 140 64 546.6 0 64 141 57 546.6 0 57 142 58 546.6 0 58 143 83 546.6 0 83 144 88 546.6 0 88 145 103 546.6 0 103 146 89 546.6 0 89 147 903 546.6 0 903 148 1512 546.6 0 1512 149 2521 546.6 0 2521 150 384 546.6 0 384 151 34 546.6 0 34 152 46 546.6 0 46 153 45 546.6 0 45 154 48 546.6 0 48 155 54 546.6 0 54 156 80 546.6 0 80 157 75 546.6 0 75 158 54 546.6 0 54 159 69 546.6 0 69 160 65 546.6 0 65 161 89 546.6 0 89 162 62 546.6 0 62 163 57 546.6 0 57 164 47 546.6 0 47 165 60 546.6 0 60 166 43 546.6 0 43 167 33 546.6 0 33 168 27 546.6 0 27 169 149 546.6 0 149 170 143 546.6 0 143 171 29 546.6 0 29 172 41 546.6 0 41 173 57 546.6 0 57 174 66 546.6 0 66 175 60 546.6 0 60 176 51 546.6 0 51 177 42 546.6 0 42 178 67 546.6 0 67 179 106 546.6 0 106 180 62 546.6 0 62 181 129 546.6 0 129 182 143 546.6 0 143 183 128 546.6 0 128 184 102 546.6 0 102 185 135 546.6 0 135 186 184 546.6 0 184 187 233 546.6 0 233 188 211 546.6 0 211 189 1127 546.6 0 1127 190 563 546.6 0 563 191 2489 546.6 0 2489 192 806 546.6 0 806 193 511 546.6 0 511 194 444 546.6 0 444 195 624 546.6 0 624 196 537 546.6 0 537 197 415 546.6 0 415 198 616 546.6 0 616 199 1485 546.6 0 1485 200 3356 546.6 0 3356 201 5436 546.6 0 5436 202 8047 546.6 0 8047 203 1573 546.6 0 1573 204 1356 546.6 0 1356 205 17186 546.6 0 17186 206 22827 546.6 0 22827 207 47587 546.6 0 47587 208 19005 546.6 0 19005 209 17191 546.6 0 17191 210 13070 546.6 0 13070 211 2239 546.6 0 2239 212 961 546.6 0 961 213 714 546.6 0 714 214 606 546.6 0 606 215 1268 546.6 0 1268 216 748 546.6 0 748 217 552 546.6 0 552 218 1237 546.6 0 1237 219 1614 546.6 0 1614 220 2663 546.6 0 2663 221 2172 546.6 0 2172 222 1989 546.6 0 1989 223 1630 546.6 0 1630 224 2310 546.6 0 2310 225 6486 546.6 0 6486 226 21782 546.6 0 21782 227 43629 546.6 0 43629 228 24656 546.6 0 24656 229 86 546.6 0 86 230 33 546.6 0 33 231 19 546.6 0 19 232 26 546.6 0 26 233 18 546.6 0 18 234 28 546.6 0 28 235 18 546.6 0 18 236 29 546.6 0 29 237 28 546.6 0 28 238 39 546.6 0 39 239 15 546.6 0 15 240 22 546.6 0 22 241 30 546.6 0 30 242 35 546.6 0 35 243 34 546.6 0 34 244 94 546.6 0 94 245 131 546.6 0 131 246 169 546.6 0 169 247 67 546.6 0 67 248 149 546.6 0 149 249 201 546.6 0 201 250 105 546.6 0 105 251 122 546.6 0 122 252 160 546.6 0 160 253 151 546.6 0 151 254 151 546.6 0 151 255 246 546.6 0 246 256 146 546.6 0 146 257 210 546.6 0 210 258 236 546.6 0 236 259 572 546.6 0 572 260 540 546.6 0 540 261 710 546.6 0 710 262 6587 546.6 0 6587 263 8239 546.6 0 8239 264 15653 546.6 0 15653 265 132 546.6 0 132 266 43 546.6 0 43 267 12 546.6 0 12 268 27 546.6 0 27 269 43 546.6 0 43 270 48 546.6 0 48 271 93 546.6 0 93 272 47 546.6 0 47 273 29 546.6 0 29 274 110 546.6 0 110 275 92 546.6 0 92 276 53 546.6 0 53 277 28 546.6 0 28 278 32 546.6 0 32 279 25 546.6 0 25 280 15 546.6 0 15 281 14 546.6 0 14 282 35 546.6 0 35 283 108 546.6 0 108 284 82 546.6 0 82 285 35 546.6 0 35 286 23 546.6 0 23 287 22 546.6 0 22 288 15 546.6 0 15 289 24 546.6 0 24 290 15 546.6 0 15 291 20 546.6 0 20 292 101 546.6 0 101 293 122 546.6 0 122 294 273 546.6 0 273 295 55 546.6 0 55 296 59 546.6 0 59 297 60 546.6 0 60 298 24 546.6 0 24 299 25 546.6 0 25 300 33 546.6 0 33 301 42 546.6 0 42 Finished in 7.18 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 168,078 Reads with adapters: 63,199 (37.6%) Reads that were too short: 107 (0.1%) Reads written (passing filters): 63,092 (37.5%) Total basepairs processed: 43,914,233 bp Total written (filtered): 17,347,967 bp (39.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 63199 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 21 0.0 3 0 0 18 3 16 517 0.0 3 0 497 15 5 17 62221 0.0 3 60795 1381 42 3 18 136 0.0 3 26 101 4 5 19 47 0.0 3 7 0 34 6 20 110 0.0 3 51 0 0 59 21 3 0.0 3 2 0 0 1 26 5 0.0 3 0 0 0 5 44 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 75 30 0.0 3 0 0 0 30 78 28 0.0 3 0 0 1 27 80 1 0.0 3 0 0 0 1 81 7 0.0 3 0 0 0 7 84 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 1 135 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 170 2 0.0 3 0 0 0 2 180 1 0.0 3 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 217 32 0.0 3 0 0 1 31 218 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 2 0.0 3 0 0 0 2 224 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 5 0.0 3 0 0 0 5 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 3 0.0 3 0 0 0 3 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.18 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 63,092 Reads with adapters: 1,115 (1.8%) Reads written (passing filters): 63,092 (100.0%) Total basepairs processed: 17,347,967 bp Total written (filtered): 17,344,560 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1115 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 57.0% C: 5.9% G: 35.4% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1072 985.8 0 1072 4 39 246.5 0 39 5 1 61.6 0 1 7 1 3.9 0 1 11 1 0.0 1 0 1 12 1 0.0 1 0 1