This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 60.27 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 434,872 (19.4%) Reads that were too short: 20,635 (0.9%) Reads written (passing filters): 414,237 (18.5%) Total basepairs processed: 673,916,425 bp Total written (filtered): 117,296,305 bp (17.4%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 434872 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3047 546.6 0 3047 7 14988 546.6 0 14988 8 190246 546.6 0 190246 9 174092 546.6 0 174092 10 228 546.6 0 228 11 29 546.6 0 29 12 34 546.6 0 34 13 14 546.6 0 14 14 23 546.6 0 23 15 12 546.6 0 12 16 13 546.6 0 13 17 29 546.6 0 29 18 23 546.6 0 23 19 12 546.6 0 12 20 24 546.6 0 24 21 15 546.6 0 15 22 23 546.6 0 23 23 23 546.6 0 23 24 25 546.6 0 25 25 37 546.6 0 37 26 7 546.6 0 7 27 38 546.6 0 38 28 13 546.6 0 13 29 26 546.6 0 26 30 22 546.6 0 22 31 18 546.6 0 18 32 47 546.6 0 47 33 52 546.6 0 52 34 142 546.6 0 142 35 46 546.6 0 46 36 51 546.6 0 51 37 222 546.6 0 222 38 76 546.6 0 76 39 98 546.6 0 98 40 53 546.6 0 53 41 37 546.6 0 37 42 322 546.6 0 322 43 11 546.6 0 11 44 26 546.6 0 26 45 19 546.6 0 19 46 30 546.6 0 30 47 23 546.6 0 23 48 29 546.6 0 29 49 42 546.6 0 42 50 19 546.6 0 19 51 29 546.6 0 29 52 467 546.6 0 467 53 107 546.6 0 107 54 94 546.6 0 94 55 16 546.6 0 16 56 18 546.6 0 18 57 22 546.6 0 22 58 19 546.6 0 19 59 19 546.6 0 19 60 41 546.6 0 41 61 44 546.6 0 44 62 39 546.6 0 39 63 26 546.6 0 26 64 12 546.6 0 12 65 33 546.6 0 33 66 63 546.6 0 63 67 30 546.6 0 30 68 52 546.6 0 52 69 18 546.6 0 18 70 22 546.6 0 22 71 43 546.6 0 43 72 38 546.6 0 38 73 68 546.6 0 68 74 12 546.6 0 12 75 21 546.6 0 21 76 21 546.6 0 21 77 20 546.6 0 20 78 20 546.6 0 20 79 17 546.6 0 17 80 23 546.6 0 23 81 45 546.6 0 45 82 54 546.6 0 54 83 44 546.6 0 44 84 297 546.6 0 297 85 65 546.6 0 65 86 86 546.6 0 86 87 22 546.6 0 22 88 25 546.6 0 25 89 21 546.6 0 21 90 95 546.6 0 95 91 45 546.6 0 45 92 73 546.6 0 73 93 117 546.6 0 117 94 65 546.6 0 65 95 107 546.6 0 107 96 28 546.6 0 28 97 142 546.6 0 142 98 15 546.6 0 15 99 16 546.6 0 16 100 20 546.6 0 20 101 14 546.6 0 14 102 11 546.6 0 11 103 29 546.6 0 29 104 64 546.6 0 64 105 62 546.6 0 62 106 74 546.6 0 74 107 134 546.6 0 134 108 811 546.6 0 811 109 84 546.6 0 84 110 160 546.6 0 160 111 63 546.6 0 63 112 209 546.6 0 209 113 116 546.6 0 116 114 34 546.6 0 34 115 23 546.6 0 23 116 31 546.6 0 31 117 88 546.6 0 88 118 50 546.6 0 50 119 53 546.6 0 53 120 21 546.6 0 21 121 21 546.6 0 21 122 14 546.6 0 14 123 18 546.6 0 18 124 44 546.6 0 44 125 49 546.6 0 49 126 76 546.6 0 76 127 450 546.6 0 450 128 86 546.6 0 86 129 148 546.6 0 148 130 46 546.6 0 46 131 45 546.6 0 45 132 92 546.6 0 92 133 46 546.6 0 46 134 33 546.6 0 33 135 27 546.6 0 27 136 39 546.6 0 39 137 58 546.6 0 58 138 78 546.6 0 78 139 102 546.6 0 102 140 217 546.6 0 217 141 296 546.6 0 296 142 483 546.6 0 483 143 1167 546.6 0 1167 144 12790 546.6 0 12790 145 2305 546.6 0 2305 146 2008 546.6 0 2008 147 31 546.6 0 31 148 27 546.6 0 27 149 62 546.6 0 62 150 36 546.6 0 36 151 348 546.6 0 348 152 240 546.6 0 240 153 202 546.6 0 202 154 333 546.6 0 333 155 107 546.6 0 107 156 220 546.6 0 220 157 93 546.6 0 93 158 123 546.6 0 123 159 107 546.6 0 107 160 28 546.6 0 28 161 34 546.6 0 34 162 16 546.6 0 16 163 27 546.6 0 27 164 21 546.6 0 21 165 38 546.6 0 38 166 25 546.6 0 25 167 26 546.6 0 26 168 18 546.6 0 18 169 19 546.6 0 19 170 33 546.6 0 33 171 53 546.6 0 53 172 72 546.6 0 72 173 50 546.6 0 50 174 33 546.6 0 33 175 33 546.6 0 33 176 20 546.6 0 20 177 57 546.6 0 57 178 40 546.6 0 40 179 278 546.6 0 278 180 117 546.6 0 117 181 79 546.6 0 79 182 18 546.6 0 18 183 21 546.6 0 21 184 29 546.6 0 29 185 42 546.6 0 42 186 45 546.6 0 45 187 29 546.6 0 29 188 35 546.6 0 35 189 29 546.6 0 29 190 185 546.6 0 185 191 27 546.6 0 27 192 109 546.6 0 109 193 31 546.6 0 31 194 30 546.6 0 30 195 27 546.6 0 27 196 48 546.6 0 48 197 35 546.6 0 35 198 157 546.6 0 157 199 88 546.6 0 88 200 52 546.6 0 52 201 43 546.6 0 43 202 27 546.6 0 27 203 30 546.6 0 30 204 28 546.6 0 28 205 59 546.6 0 59 206 52 546.6 0 52 207 31 546.6 0 31 208 28 546.6 0 28 209 23 546.6 0 23 210 15 546.6 0 15 211 25 546.6 0 25 212 29 546.6 0 29 213 26 546.6 0 26 214 32 546.6 0 32 215 14 546.6 0 14 216 28 546.6 0 28 217 32 546.6 0 32 218 32 546.6 0 32 219 52 546.6 0 52 220 26 546.6 0 26 221 29 546.6 0 29 222 17 546.6 0 17 223 28 546.6 0 28 224 25 546.6 0 25 225 78 546.6 0 78 226 52 546.6 0 52 227 43 546.6 0 43 228 75 546.6 0 75 229 83 546.6 0 83 230 126 546.6 0 126 231 63 546.6 0 63 232 59 546.6 0 59 233 67 546.6 0 67 234 67 546.6 0 67 235 382 546.6 0 382 236 676 546.6 0 676 237 306 546.6 0 306 238 199 546.6 0 199 239 136 546.6 0 136 240 377 546.6 0 377 241 123 546.6 0 123 242 114 546.6 0 114 243 40 546.6 0 40 244 42 546.6 0 42 245 71 546.6 0 71 246 88 546.6 0 88 247 222 546.6 0 222 248 113 546.6 0 113 249 198 546.6 0 198 250 160 546.6 0 160 251 131 546.6 0 131 252 329 546.6 0 329 253 89 546.6 0 89 254 47 546.6 0 47 255 59 546.6 0 59 256 68 546.6 0 68 257 69 546.6 0 69 258 153 546.6 0 153 259 347 546.6 0 347 260 3956 546.6 0 3956 261 1997 546.6 0 1997 262 2111 546.6 0 2111 263 263 546.6 0 263 264 40 546.6 0 40 265 53 546.6 0 53 266 110 546.6 0 110 267 118 546.6 0 118 268 136 546.6 0 136 269 78 546.6 0 78 270 250 546.6 0 250 271 124 546.6 0 124 272 467 546.6 0 467 273 54 546.6 0 54 274 35 546.6 0 35 275 35 546.6 0 35 276 46 546.6 0 46 277 235 546.6 0 235 278 68 546.6 0 68 279 142 546.6 0 142 280 241 546.6 0 241 281 253 546.6 0 253 282 151 546.6 0 151 283 113 546.6 0 113 284 111 546.6 0 111 285 306 546.6 0 306 286 101 546.6 0 101 287 98 546.6 0 98 288 295 546.6 0 295 289 150 546.6 0 150 290 212 546.6 0 212 291 448 546.6 0 448 292 386 546.6 0 386 293 406 546.6 0 406 294 418 546.6 0 418 295 302 546.6 0 302 296 163 546.6 0 163 297 49 546.6 0 49 298 37 546.6 0 37 299 46 546.6 0 46 300 52 546.6 0 52 301 39 546.6 0 39 Finished in 19.39 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 414,237 Reads with adapters: 182,393 (44.0%) Reads that were too short: 193 (0.0%) Reads written (passing filters): 182,200 (44.0%) Total basepairs processed: 117,296,305 bp Total written (filtered): 49,473,131 bp (42.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 182393 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 0 0 0 2 17 23 0.0 3 0 0 0 12 11 18 125 0.0 3 0 0 76 28 21 19 2351 0.0 3 0 1998 169 118 66 20 177388 0.0 4 166181 8858 1725 447 177 21 398 0.0 4 49 300 29 14 6 22 56 0.0 4 2 0 32 8 14 23 959 0.0 4 0 1 0 764 194 24 8 0.0 4 0 0 0 0 8 25 3 0.0 4 2 0 0 0 1 27 2 0.0 4 0 0 1 0 1 28 2 0.0 4 0 0 0 0 2 29 4 0.0 4 3 0 0 0 1 38 2 0.0 4 2 39 1 0.0 4 0 0 0 0 1 44 1 0.0 4 1 45 1 0.0 4 1 47 2 0.0 4 0 0 0 1 1 48 1 0.0 4 1 87 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 104 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 9 0.0 4 0 0 0 0 9 127 805 0.0 4 0 0 0 1 804 128 14 0.0 4 0 0 0 0 14 129 1 0.0 4 0 0 0 0 1 130 3 0.0 4 0 0 0 0 3 131 1 0.0 4 0 0 0 1 134 1 0.0 4 0 0 0 0 1 135 7 0.0 4 0 0 0 0 7 136 2 0.0 4 0 0 0 0 2 137 5 0.0 4 0 0 0 0 5 147 1 0.0 4 0 0 0 0 1 152 4 0.0 4 0 0 0 0 4 153 6 0.0 4 0 0 0 0 6 154 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 1 186 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 1 202 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 207 5 0.0 4 0 0 0 0 5 208 6 0.0 4 0 0 0 0 6 209 4 0.0 4 0 0 0 0 4 210 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 213 2 0.0 4 0 0 0 0 2 214 2 0.0 4 0 0 0 0 2 215 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 221 3 0.0 4 0 0 0 0 3 224 1 0.0 4 0 0 0 0 1 225 8 0.0 4 0 0 0 0 8 226 4 0.0 4 0 0 0 0 4 227 4 0.0 4 0 0 0 0 4 228 48 0.0 4 0 0 0 2 46 229 21 0.0 4 0 0 0 0 21 230 7 0.0 4 0 0 0 0 7 232 2 0.0 4 0 0 0 0 2 233 4 0.0 4 0 0 0 0 4 234 6 0.0 4 0 0 0 0 6 235 3 0.0 4 0 0 0 0 3 236 5 0.0 4 0 0 0 0 5 237 2 0.0 4 0 0 0 0 2 238 1 0.0 4 0 0 0 0 1 252 1 0.0 4 0 0 0 1 254 1 0.0 4 0 0 0 0 1 257 3 0.0 4 0 0 0 0 3 258 1 0.0 4 0 0 0 1 259 1 0.0 4 0 0 0 0 1 260 2 0.0 4 0 0 0 0 2 262 2 0.0 4 0 0 0 0 2 264 3 0.0 4 0 0 0 0 3 265 2 0.0 4 0 0 0 0 2 268 2 0.0 4 0 0 0 0 2 274 1 0.0 4 0 0 0 0 1 280 1 0.0 4 0 0 0 0 1 281 3 0.0 4 0 0 0 0 3 282 5 0.0 4 0 0 0 0 5 283 3 0.0 4 0 0 0 0 3 284 1 0.0 4 0 0 0 0 1 285 2 0.0 4 0 0 0 0 2 288 1 0.0 4 0 0 0 0 1 289 3 0.0 4 0 0 0 0 3 290 2 0.0 4 0 0 0 0 2 292 3 0.0 4 0 0 1 0 2 293 4 0.0 4 0 0 0 0 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.61 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 182,200 Reads with adapters: 41,453 (22.8%) Reads written (passing filters): 182,200 (100.0%) Total basepairs processed: 49,473,131 bp Total written (filtered): 49,020,641 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 41453 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.6% C: 8.4% G: 86.6% T: 2.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 134 2846.9 0 134 4 36 711.7 0 36 5 8 177.9 0 8 6 6 44.5 0 6 7 8 11.1 0 8 8 18 2.8 0 18 9 5149 0.7 0 589 4560 10 27914 0.2 1 2208 25706 11 7732 0.0 1 372 7360 12 130 0.0 1 2 128 13 104 0.0 1 1 103 14 5 0.0 1 0 5 16 1 0.0 1 1 22 1 0.0 1 0 1 27 2 0.0 1 0 2 29 1 0.0 1 1 40 5 0.0 1 4 1 41 1 0.0 1 1 47 1 0.0 1 1 50 2 0.0 1 1 1 83 1 0.0 1 1 88 1 0.0 1 1 112 1 0.0 1 1 138 2 0.0 1 1 1 147 3 0.0 1 2 1 148 1 0.0 1 1 149 1 0.0 1 1 150 34 0.0 1 26 8 151 2 0.0 1 1 1 155 1 0.0 1 1 157 1 0.0 1 1 161 1 0.0 1 1 169 2 0.0 1 2 170 3 0.0 1 3 177 3 0.0 1 3 189 2 0.0 1 0 2 193 1 0.0 1 1 203 1 0.0 1 1 207 1 0.0 1 1 208 115 0.0 1 87 28 209 3 0.0 1 2 1 210 2 0.0 1 2 223 3 0.0 1 3 224 2 0.0 1 2 228 1 0.0 1 1 244 1 0.0 1 1 257 5 0.0 1 5 271 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.