This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 59.25 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 436,209 (19.5%) Reads that were too short: 9,497 (0.4%) Reads written (passing filters): 426,712 (19.1%) Total basepairs processed: 673,916,425 bp Total written (filtered): 121,053,546 bp (18.0%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 436209 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1912 546.6 0 1912 7 9785 546.6 0 9785 8 179408 546.6 0 179408 9 206135 546.6 0 206135 10 145 546.6 0 145 11 14 546.6 0 14 12 20 546.6 0 20 13 11 546.6 0 11 14 8 546.6 0 8 15 6 546.6 0 6 16 6 546.6 0 6 17 16 546.6 0 16 18 5 546.6 0 5 19 14 546.6 0 14 20 13 546.6 0 13 21 9 546.6 0 9 22 13 546.6 0 13 23 11 546.6 0 11 24 12 546.6 0 12 25 9 546.6 0 9 26 9 546.6 0 9 27 16 546.6 0 16 28 7 546.6 0 7 29 22 546.6 0 22 30 6 546.6 0 6 31 8 546.6 0 8 32 29 546.6 0 29 33 20 546.6 0 20 34 144 546.6 0 144 35 20 546.6 0 20 36 25 546.6 0 25 37 190 546.6 0 190 38 41 546.6 0 41 39 29 546.6 0 29 40 20 546.6 0 20 41 10 546.6 0 10 42 69 546.6 0 69 43 7 546.6 0 7 44 9 546.6 0 9 45 13 546.6 0 13 46 9 546.6 0 9 47 12 546.6 0 12 48 6 546.6 0 6 49 20 546.6 0 20 50 11 546.6 0 11 51 17 546.6 0 17 52 163 546.6 0 163 53 48 546.6 0 48 54 39 546.6 0 39 55 4 546.6 0 4 56 18 546.6 0 18 57 14 546.6 0 14 58 14 546.6 0 14 59 10 546.6 0 10 60 217 546.6 0 217 61 78 546.6 0 78 62 28 546.6 0 28 63 11 546.6 0 11 64 7 546.6 0 7 65 12 546.6 0 12 66 13 546.6 0 13 67 8 546.6 0 8 68 13 546.6 0 13 69 8 546.6 0 8 70 9 546.6 0 9 71 10 546.6 0 10 72 14 546.6 0 14 73 36 546.6 0 36 74 9 546.6 0 9 75 7 546.6 0 7 76 13 546.6 0 13 77 8 546.6 0 8 78 17 546.6 0 17 79 10 546.6 0 10 80 14 546.6 0 14 81 28 546.6 0 28 82 46 546.6 0 46 83 36 546.6 0 36 84 259 546.6 0 259 85 38 546.6 0 38 86 29 546.6 0 29 87 10 546.6 0 10 88 16 546.6 0 16 89 7 546.6 0 7 90 40 546.6 0 40 91 26 546.6 0 26 92 28 546.6 0 28 93 28 546.6 0 28 94 24 546.6 0 24 95 32 546.6 0 32 96 23 546.6 0 23 97 119 546.6 0 119 98 12 546.6 0 12 99 14 546.6 0 14 100 17 546.6 0 17 101 11 546.6 0 11 102 10 546.6 0 10 103 11 546.6 0 11 104 40 546.6 0 40 105 30 546.6 0 30 106 33 546.6 0 33 107 47 546.6 0 47 108 244 546.6 0 244 109 34 546.6 0 34 110 35 546.6 0 35 111 22 546.6 0 22 112 66 546.6 0 66 113 45 546.6 0 45 114 27 546.6 0 27 115 26 546.6 0 26 116 17 546.6 0 17 117 89 546.6 0 89 118 30 546.6 0 30 119 12 546.6 0 12 120 10 546.6 0 10 121 10 546.6 0 10 122 9 546.6 0 9 123 16 546.6 0 16 124 28 546.6 0 28 125 35 546.6 0 35 126 46 546.6 0 46 127 403 546.6 0 403 128 92 546.6 0 92 129 120 546.6 0 120 130 42 546.6 0 42 131 48 546.6 0 48 132 95 546.6 0 95 133 33 546.6 0 33 134 25 546.6 0 25 135 12 546.6 0 12 136 30 546.6 0 30 137 68 546.6 0 68 138 49 546.6 0 49 139 102 546.6 0 102 140 239 546.6 0 239 141 302 546.6 0 302 142 485 546.6 0 485 143 1363 546.6 0 1363 144 15436 546.6 0 15436 145 2823 546.6 0 2823 146 2103 546.6 0 2103 147 18 546.6 0 18 148 18 546.6 0 18 149 18 546.6 0 18 150 11 546.6 0 11 151 119 546.6 0 119 152 65 546.6 0 65 153 51 546.6 0 51 154 60 546.6 0 60 155 20 546.6 0 20 156 80 546.6 0 80 157 39 546.6 0 39 158 74 546.6 0 74 159 85 546.6 0 85 160 10 546.6 0 10 161 14 546.6 0 14 162 8 546.6 0 8 163 8 546.6 0 8 164 18 546.6 0 18 165 15 546.6 0 15 166 6 546.6 0 6 167 9 546.6 0 9 168 5 546.6 0 5 169 8 546.6 0 8 170 14 546.6 0 14 171 16 546.6 0 16 172 24 546.6 0 24 173 25 546.6 0 25 174 18 546.6 0 18 175 13 546.6 0 13 176 13 546.6 0 13 177 19 546.6 0 19 178 37 546.6 0 37 179 132 546.6 0 132 180 113 546.6 0 113 181 34 546.6 0 34 182 19 546.6 0 19 183 9 546.6 0 9 184 10 546.6 0 10 185 19 546.6 0 19 186 26 546.6 0 26 187 12 546.6 0 12 188 16 546.6 0 16 189 29 546.6 0 29 190 197 546.6 0 197 191 15 546.6 0 15 192 73 546.6 0 73 193 16 546.6 0 16 194 15 546.6 0 15 195 12 546.6 0 12 196 21 546.6 0 21 197 14 546.6 0 14 198 54 546.6 0 54 199 42 546.6 0 42 200 25 546.6 0 25 201 13 546.6 0 13 202 13 546.6 0 13 203 8 546.6 0 8 204 8 546.6 0 8 205 13 546.6 0 13 206 14 546.6 0 14 207 8 546.6 0 8 208 7 546.6 0 7 209 6 546.6 0 6 210 10 546.6 0 10 211 13 546.6 0 13 212 7 546.6 0 7 213 14 546.6 0 14 214 9 546.6 0 9 215 12 546.6 0 12 216 19 546.6 0 19 217 36 546.6 0 36 218 15 546.6 0 15 219 23 546.6 0 23 220 10 546.6 0 10 221 9 546.6 0 9 222 11 546.6 0 11 223 13 546.6 0 13 224 16 546.6 0 16 225 37 546.6 0 37 226 23 546.6 0 23 227 17 546.6 0 17 228 28 546.6 0 28 229 34 546.6 0 34 230 64 546.6 0 64 231 27 546.6 0 27 232 35 546.6 0 35 233 34 546.6 0 34 234 29 546.6 0 29 235 180 546.6 0 180 236 291 546.6 0 291 237 103 546.6 0 103 238 60 546.6 0 60 239 37 546.6 0 37 240 155 546.6 0 155 241 54 546.6 0 54 242 62 546.6 0 62 243 16 546.6 0 16 244 18 546.6 0 18 245 37 546.6 0 37 246 24 546.6 0 24 247 74 546.6 0 74 248 36 546.6 0 36 249 108 546.6 0 108 250 40 546.6 0 40 251 44 546.6 0 44 252 120 546.6 0 120 253 18 546.6 0 18 254 15 546.6 0 15 255 12 546.6 0 12 256 23 546.6 0 23 257 30 546.6 0 30 258 71 546.6 0 71 259 162 546.6 0 162 260 1946 546.6 0 1946 261 957 546.6 0 957 262 1011 546.6 0 1011 263 166 546.6 0 166 264 15 546.6 0 15 265 21 546.6 0 21 266 59 546.6 0 59 267 51 546.6 0 51 268 62 546.6 0 62 269 52 546.6 0 52 270 105 546.6 0 105 271 78 546.6 0 78 272 298 546.6 0 298 273 22 546.6 0 22 274 15 546.6 0 15 275 15 546.6 0 15 276 23 546.6 0 23 277 141 546.6 0 141 278 30 546.6 0 30 279 57 546.6 0 57 280 70 546.6 0 70 281 58 546.6 0 58 282 47 546.6 0 47 283 112 546.6 0 112 284 117 546.6 0 117 285 516 546.6 0 516 286 42 546.6 0 42 287 48 546.6 0 48 288 160 546.6 0 160 289 53 546.6 0 53 290 94 546.6 0 94 291 122 546.6 0 122 292 83 546.6 0 83 293 119 546.6 0 119 294 77 546.6 0 77 295 78 546.6 0 78 296 45 546.6 0 45 297 25 546.6 0 25 298 26 546.6 0 26 299 21 546.6 0 21 300 21 546.6 0 21 301 27 546.6 0 27 Finished in 18.01 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 426,712 Reads with adapters: 183,939 (43.1%) Reads that were too short: 124 (0.0%) Reads written (passing filters): 183,815 (43.1%) Total basepairs processed: 121,053,546 bp Total written (filtered): 50,737,951 bp (41.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 183939 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 44 0.0 3 0 0 41 3 16 1038 0.0 3 0 1012 24 2 17 182098 0.0 3 178614 3386 90 8 18 374 0.0 3 18 349 7 19 91 0.0 3 0 0 80 11 20 140 0.0 3 0 0 1 139 21 1 0.0 3 0 0 1 22 1 0.0 3 0 0 0 1 23 1 0.0 3 1 33 1 0.0 3 1 34 1 0.0 3 1 38 2 0.0 3 2 44 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 1 84 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 137 1 0.0 3 1 142 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 4 0.0 3 0 0 0 4 169 1 0.0 3 1 178 2 0.0 3 0 0 0 2 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 0 4 194 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 208 1 0.0 3 1 216 1 0.0 3 0 0 0 1 217 70 0.0 3 0 1 2 67 219 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 224 1 0.0 3 0 1 225 1 0.0 3 0 0 0 1 270 3 0.0 3 0 0 0 3 278 1 0.0 3 0 0 0 1 279 5 0.0 3 0 0 0 5 280 10 0.0 3 0 0 0 10 281 6 0.0 3 0 0 1 5 282 7 0.0 3 0 0 1 6 283 2 0.0 3 0 0 0 2 284 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.39 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 183,815 Reads with adapters: 1,114 (0.6%) Reads written (passing filters): 183,815 (100.0%) Total basepairs processed: 50,737,951 bp Total written (filtered): 50,734,231 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1114 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 69.3% C: 8.4% G: 18.3% T: 3.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 865 2872.1 0 865 4 231 718.0 0 231 5 11 179.5 0 11 6 3 44.9 0 3 7 2 11.2 0 2 13 1 0.0 1 0 1 101 1 0.0 2 0 0 1